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Apa TNF alpha?

Human Tumor Necrosis Factor alpha (TNF , TNF alpha or TNFa) exists as a homotrimer, consisting of three 17 kDa subunits, with a molecular mass of ~51 kDa (refs 1,2). Tumor Nekrosis Faktor Manusia alfa (TNF TNF alfa atau TNFa) ada sebagai homotrimer, yang terdiri dari tiga subunit 17 kDa, dengan massa molekul ~ 51 kDa (ref 1,2). It is a pro-inflammatory, pro-apoptotic cytokine that regulates the activity of neutrophils, eosinophils, and T & B lymphocytes and modulates the properties of the vascular endothelium (ref 3). Ini adalah pro-inflamasi, sitokin pro-apoptosis yang mengatur aktivitas neutrofil, eosinofil, dan limfosit T & B dan memodulasi properti dari endotelium vaskular (ref 3). TNF alpha plays a major role in neuroinflammation-mediated cell death in Alzheimer's disease, Parkinson's disease, and stroke. TNF alpha memainkan peran utama dalam peradangan saraf-dimediasi kematian sel pada penyakit Alzheimer, penyakit Parkinson, dan stroke. Small-molecule anti-TNFa drugs are being investigated as potential therapeutics (ref 4). Obat molekul kecil anti-TNFa sedang diselidiki sebagai terapi potensial (ref 4). TNF alpha antagonists, including antibodies and soluble receptors, are being used to treat Crohn's disease, rheumatoid arthritis, and asthma (ref 5). TNF alpha antagonis, termasuk antibodi dan reseptor larut, yang digunakan untuk mengobati penyakit Crohn, rheumatoid arthritis, dan asma (ref 5). TNF alpha has also been shown to play a pathogenic role in heart failure (ref 6). TNF alpha juga telah ditunjukkan untuk memainkan peran patogenik pada gagal jantung (ref 6). TNF alpha has been implicated in both cancer development and progression in some pre-clinical models, but also possesses anti-tumor activity (ref 7,8). TNF alpha telah terlibat dalam perkembangan kanker baik dan kemajuan dalam beberapa model pra-klinis, tetapi juga memiliki aktivitas anti-tumor (ref 7,8). Because of its systemic toxicity, TNF alpha administration is limited to cases involving solid tumors confined to one extremity or organ. Karena toksisitas sistemik, TNF alfa administrasi terbatas pada kasus yang melibatkan tumor padat terbatas pada satu ekstremitas atau organ. Investigation of strategies to sensitize malignant cells to TNF alpha and maximize the selectivity of TNFa toxicity to cancer cells is ongoing (ref 8). Investigasi strategi untuk menyadarkan sel-sel ganas untuk TNF alpha dan memaksimalkan selektivitas toksisitas TNFa sel-sel kanker sedang berlangsung (ref 8).

TNFa ELISA Kits ELISA Kit TNFa Thermo Scientific TNFa Colorimetric ELISA Kits are complete, ready-to-use kits for the quantification of TNF alpha in serum, plasma, and culture supernatant samples. Thermo Scientific TNFa Colorimetric ELISA Kit yang lengkap, siap untuk menggunakan kit untuk kuantifikasi TNF alfa dalam serum, plasma, dan sampel kultur supernatan. The ELISA kits feature a simple procedure, small sample volume requirement (typically 50l per well), and fast, accurate results. Kit ELISA fitur prosedur sederhana, sampel kebutuhan volume kecil (biasanya 50l per sumur), dan cepat, hasil yang akurat. Human, mouse,

and rat ELISA kits are available in 1-plate, 2-plate, and 5-plate kit sizes. Manusia, mouse, dan ELISA kit tikus yang tersedia dalam 1-piring, 2-piring, dan 5-piring ukuran kit. The 5-plate kits offer the spacesaving convenience of bulk packaging. The 5-piring kit menawarkan kenyamanan hemat-ruang kemasan massal. Human TNFa ELISA Kits Manusia TNFa ELISA Kit Mouse TNFa ELISA Kits Mouse TNFa ELISA Kit Rat TNFa ELISA Kits Tikus TNFa ELISA Kit Porcine TNFa ELISA Kits Babi TNFa ELISA Kit

TNFa Screening Sets and ELISA Reagents Skrining TNFa Set dan ELISA Reagen We also offer a full line of ELISA development sets and reagents. Kami juga menawarkan garis penuh set pengembangan ELISA dan reagen. Thermo Scientific TNFa ELISA Screening Sets contain the core components (pre-titered coating antibody, detecting antibody, SA-HRP, TMB substrate, stop solution and calibrated standards) to conduct five 96-well colorimetric ELISAs. Thermo Scientific TNFa ELISA Set Skrining mengandung komponen inti (pra-pelapisan dianalisa antibodi, antibodi mendeteksi, SA-HRP, substrat TMB, stop solusi dan standar dikalibrasi) untuk melakukan lima 96-ELISA kolorimetri dengan baik. TNF alpha screening sets have been validated for the quantification of TNFa in 100l per well of culture supernatant. TNF alpha set skrining telah divalidasi untuk kuantifikasi TNFa di 100l per sumur dari supernatan budaya. Human TNFa ELISA Set Manusia TNFa ELISA Set Primate TNFa ELISA Set Primata TNFa ELISA Set Porcine TNFa ELISA Set Babi TNFa ELISA Set Equine TNFa ELISA Set Equine TNFa ELISA Set

TNFa ELISA MiniKits are a flexible, cost-effective solution for assaying large numbers of cell culture supernatant samples. TNFa ELISA MiniKits adalah, fleksibel solusi biaya-efektif untuk pengujian sejumlah besar sampel supernatan kultur sel. Each kit contains enough pre-titered coating and detection antibodies and calibrated standard to conduct forty (40) 96-well colorimetric ELISAs. Setiap kit mengandung cukup pra-coating dan dianalisa antibodi deteksi dan dikalibrasi standar untuk melakukan empat puluh (40) 96-ELISA kolorimetri. Human TNFa MiniKit Manusia TNFa MiniKit Mouse TNFa MiniKit Mouse TNFa MiniKit

TNF alpha ELISPOT Set TNF alpha ELISPOT Set The Thermo Scientific Human TNF alpha ELISPOT Set contains the basic components (pre-titered coating and detection antibodies and streptavidin-AP) and protocol required to develop ten colorimetric ELISPOT assays for quantitation of the number of cells secreting human TNF alpha. Thermo Scientific TNF alfa Mengatur Manusia ELISPOT berisi komponen dasar (pra-coating dan dianalisa antibodi deteksi dan streptavidin-AP) dan protokol yang diperlukan untuk mengembangkan sepuluh tes ELISPOT kolorimetri untuk kuantisasi jumlah mensekresi TNF alfa sel manusia. The ELISPOT set offers quality ELISPOT assays at a significant cost savings vs. complete kits. Set ELISPOT menawarkan tes kualitas ELISPOT pada penghematan biaya yang signifikan vs kit lengkap. Human TNF alpha Manusia TNF alfa ELISPOT Set ELISPOT Set

TNF alpha Recombinant Proteins TNF alfa rekombinan Protein

TNFa Recombinant Proteins are low-endotoxin recombinants that have been quality-tested for activity in bioassays. Protein rekombinan TNFa rendah endotoksin rekombinan yang telah kualitas diuji untuk kegiatan di bioassay. Recombinants can be used in cell culture or value-assigned to make standards for quantitative assays. Rekombinan dapat digunakan dalam kultur sel atau nilai-ditugaskan untuk membuat standar untuk tes kuantitatif. Several of our recombinant TNF alpha proteins are produced in a Pichia pastoris yeast system, which yields highly active proteins that are properly folded and glycosylated. Beberapa rekombinan protein TNF alpha kami diproduksi dalam sistem Pichia pastoris ragi, yang menghasilkan protein yang sangat aktif yang benar dilipat dan glikosilasi. Human TNFa Protein Manusia TNFa Protein Mouse TNFa Protein Mouse TNFa Protein Rat TNFa Protein Tikus TNFa Protein Bovine TNFa Protein Bovine TNFa Protein Porcine TNFa Protein Protein babi TNFa Canine TNFa Protein Canine TNFa Protein Equine TNFa Protein Equine TNFa Protein Feline TNFa Protein Feline TNFa Protein

TNF alpha Antibodies TNF alpha Antibodi TNFa Monoclonal Antibodies have been validated for ELISA. Antibodi monoklonal TNFa telah divalidasi untuk ELISA. Some have also been validated for Western blot, ELISPOT, and other applications. Beberapa juga telah divalidasi untuk Western blot, ELISPOT, dan aplikasi lainnya. Purified (unconjugated) and biotin-labeled formats are available. Dimurnikan (konjugasi) dan biotin berlabel format yang tersedia. Human TNFa MAbs Manusia TNFa mAbs Mouse TNFa MAbs Mouse TNFa mAbs Bovine TNFa MAb Bovine TNFa MAb Porcine TNFa MAbs Babi TNFa mAbs

Each TNF alpha Polyclonal Antibody is available in purified form, and several have been validated for Western blotting, ELISA, neutralization and other applications. Setiap TNF alfa Antibodi Poliklonal tersedia dalam bentuk dimurnikan, dan beberapa telah divalidasi untuk Western blotting, ELISA, netralisasi dan aplikasi lainnya. Human TNFa PAb Manusia TNFa PAB Mouse TNFa PAb Mouse TNFa PAB Rat TNFa PAb Tikus TNFa PAB Bovine TNFa PAb Bovine TNFa PAB Equine TNFa PAbs Equine TNFa PAbs

Related Products Produk Terkait Endogen ELISA Kits and Reagents for other cytokines, chemokines and growth factors, neuroproteins and T-cell receptors are also available. ELISA Kit endogen dan Reagen untuk sitokin lainnya, kemokin dan faktor pertumbuhan, neuroproteins dan T-sel reseptor juga tersedia. ExcelArray Antibody Sandwich Microarrays are glass slide arrays for fluorescent measurement of 12 different analytes in 10 samples per slide. Antibodi mikroarray Sandwich ExcelArray adalah array slide kaca untuk pengukuran neon 12 analit yang berbeda dalam 10 sampel per slide. Convenient kits of popular combinations of analytes are available, as well as a slide-reading service. Kit nyaman kombinasi populer analit yang tersedia, serta layanan slide-membaca.

Interleukin-6 (IL-6) Assay Kits and Reagents for detection and quantitation of IL-6 in serum, plasma, and culture supernatant samples. Interleukin-6 (IL-6) Assay Kit dan Reagen untuk deteksi dan kuantisasi IL-6 dalam serum, plasma, dan sampel kultur supernatan. Interferon gamma (IFNg) Assay Kits and Reagents for detection and quantitation of interferon gamma. Interferon gamma (IFNg) Assay Kit dan Reagen untuk deteksi dan kuantisasi gamma interferon. PHF-tau Antibodies - monoclonal antibodies detect differentially phosphorylated tau protein. PHFtau Antibodi - antibodi monoklonal mendeteksi protein tau diferensial terfosforilasi.

References Referensi

1. Utsumi, T., et al. Utsumi, T., dkk. (1992). (1992). The role of amino functions in recombinant
human tumor necrosis factor in expression of biological activity. Peran fungsi amino dalam rekombinan faktor nekrosis tumor manusia dalam ekspresi aktivitas biologis. Mol. Mol. Immunol. Immunol. 29(1), 77-81. 29 (1), 77-81. Arakawa, T., et al. Arakawa, T., dkk. (1987). (1987). Molecular weight of recombinant human tumor necrosis factor-alpha. Berat molekul rekombinan tumor necrosis factor-alpha manusia. J. Biol. J. Biol. Chem. Chem. 262(16), 7484-7485. 262 (16), 7484-7485. Balkwill, FR, (1989). Balkwill, FR, (1989). Tumour necrosis factor. Tumor necrosis factor. Br. Br. Med. Med. Bull. Bull. 45(2), 389-400. 45 (2), 389-400. Tweedie, D., et al. Tweedie, D., dkk. (2007). (2007). TNF-alpha inhibition as a treatment strategy for neurodegenerative disorders: new drug candidates and targets. TNF-alfa hambatan sebagai strategi pengobatan untuk gangguan neurodegenerative: kandidat obat baru dan target. Curr. Curr. Alzheimer Res. Alzheimer Res. 4(4), 378-385. 4 (4), 378-385. Lin, J., et al., (2008). Lin, J., et al, (2008).. TNFalpha blockade in human disease: an overview of efficacy and safety. TNFalpha blokade dalam penyakit manusia: gambaran efikasi dan keamanan. Clin. Clin. Immunol. Immunol. 126(1), 13-30. 126 (1), 13-30. Chen, D. et al. Chen, D. et al. (2008). (2008). Cytokines and acute heart failure. Sitokin dan gagal jantung akut. Crit. Crit. Care Med. Perawatan Med. 36(1 Suppl), S9-16. 36 (1 Suppl), S9-16. Mocellin, S., et al. Mocellin, S., dkk. (2008). (2008). TNF and cancer: the two sides of the coin. TNF dan kanker: dua sisi mata uang. Front. Depan. Biosci. Biosci. 13, 2774-2783. 13, 27742783. Pilati, P., et al. Pilati, P., et al. (2008). (2008). Strategies to enhance the anticancer potential of TNF. Strategi untuk meningkatkan potensi antikanker TNF. Front. Depan. Biosci. Biosci. 13, 3181-3193. 13, 3181-3193.

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