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Absorbance540

(A540)
0
0.212
0.158
0.196
0.169
0.115
0.128

Acid-hydrolized sucrose
0.25
0.2
Absorbance540

Amount of AcidHydrolyzed Sucrose


(mg/mL)
0
0.0033
0.0067
0.001
0.0133
0.0167
0.02

0.15
0.1
0.05
0
0

0.005
Amount of Acid

hydrolized sucrose (mg/mL) vs. A540

Acid-hydrolized sucrose (mg/mL


0.25

Absorbance540

0.2

0.005

0.01

0.015

0.02

Amount of Acid-Hydrolyzed Sucrose (mg/mL)

0.025

0.15
0.1
0.05
0
0

0.005
Amount of Acid

intercept=0.1179

hydrolized sucrose (mg/mL) vs. A540


y = 9.5143x

0.01

0.015

0.02

Amount of Acid-Hydrolyzed Sucrose (mg/mL)

0.025

Amount of Acid-Hydrolyzed
Sucrose (mg/mL)

A540

1
3
5
7
9
11

0.286
0.518
0.39
0.481
0.502
-0.011

0.33
0.344
0.288
0.328
0.337
0.133

pH

Amount of Acid-Hydrolyzed
Sucrose (mg/mL)

A540

1
3
5
7
9
11

0.014
0.0303
0.0347
0.0345
0.0354
0.0362

0.133
0.288
0.33
0.328
0.337
0.344

pH vs. Acid-Hydrolyzed
Amount of Acid-Hydrolyzed Sucrose
(mg/mL)

pH

0.0347

0.6
0.5
0.4
0.3
0.2
0.1
0
-0.1

0.33
Data is not accurate

0.014

0.133

y-a
x=

Hydrolyzed Sucrose (mg/mL)

10

Amount of Acid-Hydrolyzed Sucrose


(mg/mL)

pH vs. Acid

12

pH

Data is not accurate

pH 1 =
0.330/9.
5143 =
0.0347
mg/mL
pH 3 =
0.288/9.
5143 =
0.0303
mg/mL
pH 5 =
0.133/9.
5143 =
0.0140
mg/mL

x=

b-y
m

x=

y-a
m

0.04
0.035
0.03
0.025
0.02
0.015
0.01
0.005
0
0

pH 7 =
0.328/9.
5143 =
0.0345
mg/mL
pH 9 =
0.337/9.
5143 =
0.0354
mg/mL
pH 11 =
0.344/9.
5143 =
0.0362
mg/mL

pH vs. Acid-Hydrolyzed Sucrose (mg/mL)

6
pH

10

12