Sheet1 ANALISIS KADAR PROTEIN 1. Data bahan uji Berat sampel = 1.5012 2.

Standarisasi Larutan HCL Volume HCL pekat = Volume larutan HCL = volume lar boraks = 3. Standarisasi larutan NaOH Massa NaOH = Volume NaOH = NO 2.1 250 25 NO 1.047 250 1 2 Massa boraks Vol. Hcl 1 202.5 11.1 2 200.9 11

Vol. NaOH 10 10

Vol. Hcl 9 9.2

4. Titrasi Asam Penangkap Volum Asam Penangkap No Mula-mula akhir Vol. NaOH 1 75 150 56.2 2 75 150 56 1. Standarisasi HCL N1 = 2 W/ V1.mr N1= N2 = N rata-rata =

0.095672146 0.095779093 0.09572562

2. Standarisasi Larutan NaOH mgrek NaOH = mgrek Hcl N2 = N1.V1/V2 N2-1 = N2-2 = N2 rata-rata= 0.086153058 0.08806757 0.087110314

3. Penentuan kadar protein mgrek Hcl penangkap mula-mula = V. Hcl mula x N Hcl 7.179421482

sisa larutan hasil distilasi = V NaOH x NaOH untuk titrasi 4.895599646 jumlah mgrek NH3 hasil distilasi = (V Hcl mula-mula x N Hcl) (V NaOH x N NaOH) Jadi = 2.283821836 jumlah mgrek NH3 hasil distilasi = jumlah N total

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Sheet1 Berat N total dalam bahan = jumlah N total x berat atom N dimana berat atom N =14 mgram/mmol Perhitungan = 31.97350571 mgram Faktor konversi protein : F = gram protein/ gram N rata-rata protein Perhitungan = 6.25

Berat protein dalam bahan (Wp) = berat N total dalam bahan F Perhitungan = 199.8344107 kadar protein dalam bahan P dicari dengan: P = berat protein dalam bahan/ berat bahan Perhitungan = 13.31164473 % Penentuan berat protein teoritis = 1cc =0,1 Ngram = 0.008755 grek H2SO4 yang bereaksi = grek NH3 yang terbentuk Perhitungan = 0.022838218 massa protein yang didapat = grek H2SO4 yang beraksi x (0.00875 x 1000) Perhitungan == 0.199834411 gram 199.8344107 mgram Perhitungan kesalahan relatif kesalahan relatif = (Wp mref)/mref x 100% kesalahan relatif = (Wp mref)/mref 0 % x 100% Kadar protein referensi =( kadar protein percobaan-kadar protein referensi)/kadar protein referensi x 100% kadar protein referensi =( kadar protein percobaan-kadar protein referensi)/kadar protein referensi x 100% kadar protein refernsi =8.3% (www.ubb.ac.id) kesalahan relatif percobaan = 60.38126179 %

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Sheet1

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Sheet1

otein referensi x 100%

tein referensi x 100%

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