Perhitungan Faktor Retensi

Rf senyawa murni parasetamol

jarak elusi (Dm)
7
Dr
2.3
Rf
Dr/Dm
0.328571
hRf
32.85714
Rf senyawa murni kafein
jarak elusi (dM)
7
Dr
5.2
Rf
0.742857
hRf
74.28571

Data kurva baku parasetamol

konsentrasi (ppm)

luas area kromatogram

60
80
100

71149.1
101922.7
135599.3

SUMMARY OUTPUT
Regression Statistics
Multiple R
R Square
Adjusted R Square
Standard Error
Observations

0.999662033
0.999324181
0.998648361
1185.144787
3

ANOVA
df
Regression
Residual
Total

SS
MS
1 2076914140.0200 2076914140.0200
1
1404568.167
1404568.167
2
2078318708

Intercept
konsentrasi (ppm)

Coefficients
-26010.03333
1611.255

SD INTERSEPT
YLOD
LOD

5925.723936
-8232.861525
11.03312127

Standard Error
3421.21831
41.90119579

t Stat
-7.602564636
38.45367584

F
1478.685185

P-value
0.08325953
0.016551768

Significance F
0.016551768

Lower 95%
Upper 95% Lower 99.0% Upper 99.0%
-69480.73362 17460.66695 -243793.642
191773.575
1078.849828 2143.660172 -1056.03857
4278.548574

Data kuva baku parasetamol

konsentrasiluas
(ppm)
area kromatogram
60 71149.1
80 101922.7
100 135599.3

kurva baku parasetamol

160000

y = 1,611.2550x - 26,010.0333
R = 0.9993

140000
120000

kurva baku parasetamol

100000
80000

Linear (kurva baku

parasetamol)

60000
40000

Linear (kurva baku

parasetamol)

20000
0
-20000 0
-40000

20

40

60

80

100

120

Data kurva kafein

konsentrasi (ppm)

luas area kromatogram

60
276722.9
80
411854.2
100
540927.4

SUMMARY OUTPUT
Regression Statistics
Multiple R
R Square
Adjusted R Square
Standard Error
Observations

0.999912384
0.999824775
0.999649551
2473.208968
3

ANOVA
df
Regression
Residual
Total

Intercept
konsentrasi (ppm)

SD INTERSEPT
YLOD
LOD

SS
MS
1 34902008910.1250 34902008910.1250
1
6116762.602
6116762.602
2
34908125673

Coefficients
-118574.1667
6605.1125

12366.04484
-81476.03214
5.616578753

Standard Error
7139.539319
87.44114164

t Stat
-16.60809772
75.53781179

F
5705.96101

Significance F
0.008427337

P-value
0.038285667
0.008427337

Lower 95%
-209290.615
5494.067452

Upper 95%
Lower 99.0%
-27857.71838
-573053.973
7716.157548
1038.89438

Upper 99.0%
335905.6397
12171.33062

Data kurva kafein

konsentrasiluas
(ppm)
area kromatogram
60 276722.9
80 411854.2
100 540927.4

kurva baku kafein

600000

y = 6,605.1125x - 118,574.1667
R = 0.9998

500000
400000

kurva baku kafein

300000
200000

Linear (kurva baku

kafein)

100000
0
-100000
-200000

20

40

60

80

100

120

Interpolasi kuva baku parasetamol

konsentrasi (ppm) luas area kromatogram luas area x 1/B
60
71149.1
44.15756662
80
101922.7
63.25671604
100
135599.3
84.15756662

B
1611.255
1611.255
1611.255

SUMMARY OUTPUT
Regression Statistics
Multiple R
R Square
Adjusted R Square
Standard Error
Observations

0.999662033
0.999324181
0.998648361
0.735541418
3

ANOVA
df
Regression
Residual
Total

Intercept
konsentrasi (ppm)

SS

1
1
2

MS

F
Significance F
800
800 1478.685 0.016552
0.541021177 0.541021
800.5410212

Coefficients
Standard Error
t Stat
P-value Lower 95% Upper 95%
-16.14271691
2.123325178 -7.60256 0.08326 -43.1221 10.83669
1
0.026005316 38.45368 0.016552 0.669571 1.330429

Lower 99.0%
Upper 99.0%
-151.307 119.0212
-0.65541 2.655414

Interpolasi kuva baku parasetamol

konsentrasi (ppm)
luas area kromatogram
luas area x 1/B
60
71149.1
44.15756662
80
101922.7
63.25671604
100
135599.3
84.15756662

B
1611.255
1611.255
1611.255

konsentrasi parasetamol vs luas area/B

y = x - 16.1427
R = 0.9993

100
80
60

konsentrasi parasetamol
vs luas area/B

40

Linear (konsentrasi
parasetamol vs luas
area/B)

20
0
-20
-40

20

40

60

80

100

120

Interpolasi kuva baku kafein

konsentrasi (ppm)
luas area kromatogram luas area x 1/B
B
60
276722.9
41.89525916 6605.113
80
411854.2
62.3538509 6605.113
100
540927.4
81.89525916 6605.113
SUMMARY OUTPUT
Regression Statistics
Multiple R
R Square
Adjusted R Square
Standard Error
Observations

0.999912384
0.999824775
0.999649551
0.374438584
3

ANOVA
df
Regression
Residual
Total

Intercept
konsentrasi (ppm)

SS

1
1
2

MS

F
Significance F
800
800 5705.961 0.008427
0.140204253 0.140204
800.1402043

Coefficients
Standard Error
t Stat
P-value Lower 95% Upper 95%
-17.95187692
1.080911085 -16.6081 0.038286 -31.6862
-4.2176
1
0.013238403 75.53781 0.008427 0.83179 1.16821

Lower 99.0%
Upper 99.0%
-86.7592 50.8554
0.157286 1.842714

Interpolasi kuva baku kafein

konsentrasi (ppm) luas area kromatogram luas area x 1/B B
60
276722.9
41.89525916 6605.113
80
411854.2
62.3538509 6605.113
100
540927.4
81.89525916 6605.113

konsentrasi kafein vs luas area/B

100
y = x - 17.9519
R = 0.9998

80
60
40

konsentrasi kafein vs luas

area/B

20

Linear (konsentrasi
kafein vs luas area/B)

0
-20
-40

50

100

150

Perhitungan kadar sampel

kafein
sampel
luas area kromatogram
luas area terkoreksi
B
kadar
1
77755.8 11.7721
6605.1125
29.724
2
83739 12.6779
6605.1125 30.6298
3
93274.1 14.1215
6605.1125 32.0734

17.9519
17.9519
17.9519

y = x - 17,9519
x = y + 17,9519
rata-rata
SD
SE
LE
Rentang kadar
CV

30.80905674
1.1849
0.6841
2.9417
27.8673 <X<
0.03845947

33.7508

Perhitungan kadar sampel

parasetamol
sampel
luas area kromatogram
luas area terkoreksi
B
kadar
1
114972.3 71.3557
1611.255 87.4984
2
117848.6 73.1409
1611.255 89.2836
3
111052.8 68.9232
1611.255 85.0659
y = x - 16,1427
x = y + 16,427
rata-rata
SD
SE
LE
Rentang kadar
CV

87.28262923
2.1171
1.2223
5.256
82.0266 <X<
0.024255685

92.5386

16.1427
16.1427
16.1427

Parameter Kinetika Pemisahan

Faktor Kapasitas
k'
(dm-dr)/dm
Parasetamol
jarak elusi (Dm) 7
Dr
2.3
k'
0.6714

kafein
jarak elusi (dM) 7
Dr
5.2
k'
0.2571

Faktor Selektivitas ()

k' parasetamol/k'kafein

2.6111
Faktor Resolusi (Rs)
Rs
(dr kafein-dr parasetamol)/0.5(W kafein-W parasetamol)
Sampel I