I.

Data pengamatan dan perhitungan

Kromatografi Lapis Tipis a. Bercak 1 = 7.5 cm Rf =

Spektrometeri UV Visible a. Pembuatan Rodhamin Baku BPFI = b. Pembuatan Variasi Konsentrasi 1. 6 ppm V1 x 500 ppm = 25 ml x 6 ppm V1 = 0.3 ml 2. 8 ppm V1 x 500 ppm = 25 ml x 8 ppm V1 = 0,4 ml 3. 10 ppm V1 x 500 ppm = 25 ml x 10 ppm V1 = 0.5 ml 4. 12 ppm V1 x 500 ppm = 25 ml x 12 ppm V1 = 0.6 ml 5. 14 ppm V1 x 500 ppm = 25 ml x 6 ppm V1 = 0.7 ml
Tabel hasil absorbansi pada spektrofotometer uv - visible Baku C(X) 6 PPM 8 PPM 10 PPM 12 PPM 14 PPM 10.000PPM A(Y) 0.2026 0.2482 0.3101 0.3778 0.4659 0.6165

Sampel

Tabel absorbansi
0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 6 PPM 8 PPM 10 PPM 12 PPM 14 PPM 10000 PPM Baku Sampel

Y = ax + b

b = -7.18 . 10-3 -7.18 . 10-3

a = 0.6562