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Activity of Protein from Lumbricus terrestris as An Antimicrobial Toward Staphylococcus aureus Abstract

BACKGROUND AND AIM There evidence that protein from Lumbricus terrestris had an activity toward bacteria Salmonella typhii. This research were done as a preminilary research toward Staphylococcus aureus which were more weaker and nonpathogenic than Salmonella typhii METHOD Protein were extracted from Lumbricus terrestris. Then the protein were fractionated with Gel Filtration Chromatography. The each of 30 fraction obtained from fractionation were examined by using UV-Vis spectrophotometry detector. Three fraction with the highest absorbance were taken and tested toward bacteria Staphylococcus aureus with disc diffusion method in two different petri dish. RESULTS Fraction 2, 3 and 6 with the highest absorbance were tested toward bacteria Staphylococcus aureus. Fraction number 2 gave lysis zone with diameter 1,03 cm. CONCLUSIONS Protein from Lumbricus terrestris only had a little effect as an antimicrobial toward Staphylococcus aureus.