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ABSTRAK

Mahendra Karda, I Wayan Arya. 2012. Efek Ekstrak Etanol Daun Tekelan (Chromolaena odorata) Terhadap Agregasi Platelet pada Tikus Putih (Rattus norvergicus L.) Strain Wistar. Tugas Akhir, Fakultas Kedokteran, Universitas Brawijaya. Pembimbing: (1) dr. Maimun Z.A, M.Kes, SpPK (2) Dr. drg. Nur Permatasari, MS. Trombosis adalah suatu keadaan patologis dimana terjadi pembentukan bekuan darah (trombus) pada pembuluh darah yang sehat, atau terjadi setelah pembuluh darah mengalami cedera yang cukup ringan. Ekstrak etanol daun tekelan (Chromolaena odorata) mengandung senyawa flavonoid jenis quercetin yang mampu menurunkan insiden kejadian penyakit kardiovaskuler. Penelitian ini bertujuan untuk memperoleh bukti bahwa pemberian ekstrak etanol daun tekelan mampu menghambat agregasi platelet pada tikus putih (Rattus norvergicus L.) strain Wistar. Daun tekelan diperoleh dari UPT Materia Medika Batu dan diekstraksi menggunakan larutan etanol 80%. Penelitian ini menggunakan desain Randomized Posttest Only Controlled Group untuk mengamati penurunan agregasi platelet selama 30 hari yang dilaksanakan di Laboratorium Farmakologi Fakultas Kedokteran Universitas Brawijaya dan Laboratorium Sentral RSUD Dr. Saiful Anwar Malang. Dua puluh ekor tikus dibagi secara random menjadi empat kelompok perlakuan masing masing lima ekor. Perlakuan penelitian tersebut adalah 0, 100, 250, dan 500 mg/kgBB ekstrak etanol daun tekelan yang diberikan peroral. Sampel darah diambil sebelum dan sesudah perlakuan melalui vena ekor tikus untuk dibuat hapusan darah tepi. Analisa data menggunakan uji One Way ANOVA. Dari hasil penelitian diperoleh bahwa pemberian ekstrak etanol daun tekelan tidak memberikan perbedaan yang signifikan antar perlakuan (P>0.05). Dapat disimpulkan hipotesis bahwa pemberian ekstrak etanol daun tekelan (Chromolaena odorata) mampu menghambat agregasi platelet pada tikus Wistar tidak terbukti. Kata kunci: Ekstrak daun tekelan, agregasi platelet, Tikus Wistar, Trombosis

ABSTRACT

Mahendra Karda, I Wayan Arya. 2012. Effects of Ethanol Extract of Tekelan Leaf (Chromolaena odorata) on Platelet Aggregation of White Rat (Rattus norvergicus L.) Strain Wistar. Final Assignment, Faculty of Medicine, Universitas Brawijaya. Supervisors: (1) dr. Maimun ZA, M.Kes, SpPK (2) Dr. drg. Nur Permatasari, MS. Thrombosis is a pathological condition in which blood clotting occurs in healthy blood vessels or occurs after a relatively minor blood vessel injury. Ethanol extract of tekelan leaf (Chromolaena odorata) contain flavonoid substances quercetin which has the ability to reduce cardiovascular incidence. This research was conducted to study effects of ethanol extract of tekelan leaf for its capacity to inhibit platelet aggregation in white rat. The leaf extract was prepared by macerating leaf powder in 80% ethanol solution for overnight and then evaporated sufficiently until the extract was obtained. At the commencement of the research, 20 rats were grouped randomly into four of five animals each and were drenched with 0, 100, 250, and 500 mg/kg BW leaf extract orally according to treatment imposed. The experimental period lasted for 30 days which was held in The Laboratory of Pharmacology Faculty of Medicine, Universitas Brawijaya. Blood samples were taken from each rat before and after treatment to study the platelet aggregation by blood smear preparation which was done in Central Laboratory of Saiful Anwar Hospital Malang. Randomized Posttest Only Control Group was used as design of this research and data obtained were analyzed using One Way ANOVA test. The result showed that there was no significant diference between groups (P>0.05). It could be concluded that the hypothesis of anti aggregation effect of ethanol extract of tekelan leaf has not been proved. Key words: Tekelan leaf extract, platelet aggregation, white Rat Wistar thrombosis