ANTIBIOTIC SUSCEPTIBILITY TEST

Principle: Zones of growth inhibition surrounding

each type of disc were correlated with the minimum inhibitory concentrations of each antimicrobial agent.

Significant: antibiotic susceptibility testing is often

essential in order to determine which antibiotics to use against a specific strain of bacterium. A culture and susceptibility test is the growth of a known pathogen in pure culture and its exposure to known antibiotics of different concentration to determine which drug will kill or inhibit the growth of the microorganism.

PROCEDURE:

1. Prepare one agar plate for each specimen provided. Label properly. 2. Inoculate the agar plates. Streak the microorganism (e.g.S. aureus and E. coli) on the plate , overlapping the streaks to assure complete coverage of the entire agar surface with inoculum. 3. Using flamed forceps, place the antibiotic discs on the agar surface . Gently press down the discs to ensure contact with the agar. 4. Incubate the plates in an inverted manner at 37 degrees for 48 hours. 5. Measure the clearing zone or zone of inhibition with your rulers. 6. Record and tabulate your results in millimeters.

Susceptibility Testing Methods

Innoculate MH plate

Place disks on agar plate

Incubate plate 18-24 hr, 35 C Measure and record zone of inhibition around each disk

Disk Diffusion Susceptibility Testing

Improper agar & disk placement Mueller Hinton agar & good disk placement

Use Mueller Hinton agar

Microbial Antibiotic Susceptibility Test The agar diffusion test

Zones of growth inhibition

Measuring Zones of Inhibition After overnight incubation, examine the plates (keep them covered at all times). Measure the diameter of the zone of inhibition around each disk. Keeping the lid of the plate in place, use a ruler to measure the diameter of the clear area in millimetres.
Diameter of zone of inhibition (mm)
Resistant 10 or less

Intermediate Susceptible

1115 16 or more

Diameter of zone of inhibition (mm)

Resistant
Intermediate Susceptible

10 or less
1115 16 or more

GOODLUCK ON YOUR MICBIO LAB EXAM LATER

ASEPTIC TECHNIQUE GRAM STAINING ACID FAST STAINING STERILIZATION STREAKING TECHNIQUE ANTIBIOTIC SUSCEPTIBILITY