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EFEK SITOTOKSIK DAN ANTIPROLIFERATIF EKSTRAK ETANOL UMBI UBI JALAR UNGU (Ipomoea batatas L) TERHADAP SEL LINE KANKER PAYUDARA T47D
1 1 1 2 3 I Wa y a nS u ma r d i k a , Ag u n gW i wi e kI n d r a y a n i , IM a d eJ a wi , De waNg u r a hS u p r a p t a , Los e nAd n y a n a 1 2 Ba g i a nF a r ma k ol og i F K Un u d ,F a k u l t a sP e r t a n i a nUn u d , 3 Di v i s i He r ma t ol og i On k ol og i Me d i kI l muP e n y a k i t Da l a mF K Un u d Ema i l : s d i k a r ox z @g ma i l .c om

ABS TRACT Ca n c e ri ss t i l lon e oft h el e a d i n gd e a t hc a u s e swor l d wi d e . Con s u mi n ga n t i ox i d a n t sc a nr e d u c et h ec a n c e ri n c i d e n c e . An t h oc y a n i nt h a tc on t a i n e di np u r p l es we e tp ot a t oe sc a ni n h i b i tDNA d a ma g e s .I nor d e rt op r ov et h ea c t i v i t yofp u r p l es we e t p ot a t oe sonc a n c e rc e l l , i t i sn e c e s s a r yt oh a v es omed i r e c t r e s e a r c honc a n c e rc e l l l i n e . Th i ss t u d yi sas i mp l ee x p e r i me n t a lr e s e a r c hme t h od . Th ea c t i v i t i e soft h ea n t i c a n c e ra r ee v a l u a t e df r om c y t ot ox i ca n d a n t i p r ol i f e r a t i v ee f f e c t sonc e l ll i n eb r e a s tc a n c e rT47 D. Cy t ot ox i ce f f e c t si se x a mi n e dwi t hc u l t u r e dc e l ls t a i n e dwi t ht r y p h a n b l u ee x l u s i on . Ea c hwe l l a r ec on t a i nof 36 , 000 c e l l s , wh i c hi sg i v e ne t h a n ol e x t r a c t p u r p l es we e t p ot a t oe swi t h10 d os ev a r i a t i on s t a r t i n gf r om 500 g / mLu pt o 10, 000 g / mLr e p l i c a t e dt h r e et i me s , t h e nt h ei n h i b i t or yp e r c e n t a g ei sc a l c u l a t e d . An t i p r ol i f e r a t i v e a c t i v i t yi se v a l u a t e db yi n c u b a t i n gc a n c e rc e l l st h a t h a v eb e e ng i v e ne t h a n ol e x t r a c t p u r p l es we e t p ot a t oe si n3 d os e sv a r i a t i on ; 500, 1, 000 a n d2, 000 g / ml i n24, 48 a n d7 2h ou r s . Re s u l toft h i ss t u d ys h owe dt h a te t h a n ole x t r a c tp u r p l es we e tp ot a t oe sh a dc y t ot ox i ca c t i v i t i e son T47 Db r e a s tc a n c e r c e l ll i n ei nt h e s ed os ev a r i a t i on s ,500;1, 000;2, 000;3, 000;4, 000;5, 000;6 , 000;7 , 000;8, 000;9, 000 a n d 10, 000 g / ml . Th e c y t ot ox i ca c t i v i t yone a c hofd os ev a r i a t i on sa b ov ea r e27 .56 ; 42.6 7 ; 57 .7 8; 6 6 .6 7 ; 7 2.44; 7 9.56 ; 85.33; 87 .56 ; 92.44; 100; a n d 100 %. Et h a n ol e x t r a c t p u r p l es we e t p ot a t oe ss h owe da n t i p r ol i f e r a t i v ea c t i v i t i e sonT47 Db r e a s t c a n c e rc e l l si n24, 48, 7 2h ou r s i n c u b a t i on i nc on c e n t r a t i on s500;1, 000;a n d 2, 000 g / ml ;t h os ea r e35, 7 00;29, 800;25, 500 c e l l s(24 h ou r s );7 2, 500;6 0, 300; 52, 6 00 c e l l s(48 h ou r s );149, 500; 122, 6 00; 107 , 300 c e l l s(7 2h ou r s ). I nc on c l u s i on ,e t h a n ole x t r a c tp u r p l es we e tp ot a t oe sh a d c y t ot ox i ca n da n t i p r ol i f e r a t i v ea c t i v i t i e sonc e l l l i n eb r e a s t c a n c e r T47 D. Ke y wor d s : e t h a n ol , e x t r a c t p u r p l es we e t p ot a t oe s , b r e a s t c a n c e r

P ENDAHULUAN Ka n k e rd i t a n d a id e n g a np e r t u mb u h a ns e ly a n g t i d a k t e r k e n d a l i ,y a n g d a p a t me n g i n v a s ij a r i n g a n s e k i t a r n y ad a nb e r me t a s t a s i sk eb a g i a nt u b u hy a n gl a i n . 1 Ke ma t i a nme r u p a k a na k i b a t p a l i n gu t a mad a r i k a n k e r . Ka n k e r me r u p a k a np e n y a k i t p e n y e b a bk e ma t i a nk e d u a d i d u n i as e t e l a hp e n y a k i t j a n t u n gd a np e mb u l u hd a r a h .2 I n s i d e nk a n k e rd iI n d on e s i ad i p e r k i r a k a n 100 or a n g

p e r s e r i b up e n d u d u kp e r t a h u na t a us e k i t a r 200.000 p e n d u d u kp e rt a h u n . P a d as u r v e ik e s e h a t a nr u ma h t a n g g ay a n gd i s e l e n g g a r a k a n Ba d a nP e n e l i t i a nd a n P e n g e mb a n g a n Ke s e h a t a n (Li t b a n g k e s ), d i t e mu k a n 1, 4% d a r is e l u r u hk e ma t i a nd i s e b a b k a n ol e hk a n k e r . An g k ai n ime n i n g k a tme n j a d i3, 4%p a d at a h u n 1980 a n t a r as e p u l u hk a n k e r d a n4, 3%p a d at a h u n1986 .3 Di p r i me rp a d a wa n i t ad iI n d on e s i a ,k a n k e rl e h e rr a h i m me n e mp a t i p os i s i p e r t a ma(28, 6 6 %), k e mu d i a nd i i k u t i 25

Ef e kS i t ot ok s i kd a nAn t r ol i f e r a t i fEk s t r a kEt a n ol Umb i Ub i J a l a rUn g u(Ipomoea batatas L) T e r h a d a pS e l Li n eKa n k e rP a y u d a r aT47 D I Wayan Sumardika, Agung Wiwiek Indrayani, I Made Jawi, Dewa Ngurah Suprapta, Losen Adnyana

ol e hk a n k e r p a y u d a r a(17 , 7 7 %).4 T i g ap u l u hp e r s e nd a r i s e l u r u hk a n k e rp a d awa n i t aa d a l a hk a n k e rp a y u d a r a .5 Ka n k e rp a y u d a r a me r u p a k a n k a n k e ry a n g p a l i n g s e r i n gd i d e r i t awa n i t aAme r i k aS e r i k a t d a nme r u p a k a n p e n y e b a bk e ma t i a na n t a r au mu r20 s a mp a id e n g a n59 7 t a h u n .6, An g k ah a r a p a nh i d u pa k a nme n i n g k a t s e b e s a r 80% j i k ak a n k e ri n i d i t e r a p i s e c a r ad i n i .8 P e n e l i t i a ne p i d e mi ol og i me n u n j u k k a na d a n y a h u b u n g a na n t a r ak e j a d i a nk a n k e rd e n g a n ma k a n a n . Da t ai n i me n y e b a b k a nt i mb u l n y ap e mi k i r a nb a h wa d a l a m ma k a n a n t e r t e n t u t e r k a n d u n g b a h a n y a n g d a p a tme n c e g a ht e r j a d i n y ak a n k e r . S a l a hs a t ub a h a n y a n gb a n y a kd i t e l i t ia d a l a hb a h a na n t i ok s i d a nd a l a m ma k a n a n .8 An t i ok s i d a na d a l a hb a h a ny a n gd a p a t me n a n g k a p e l e k t r on b e b a sy a n gd i l e p a s k a n ol e hr a d i k a lb e b a s t a n p a me n y e b a b k a n i n s t a b i l i t a sp a d a mol e k u li t u s e n d i r i , s e h i n g g ad a p a t me n g u r a n g i k e r u s a k a nDNA.10 De n g a nd e mi k i a nd i a s u ms i k a nb a h wa on k og e n e s i s p a d af a s es a n g a t a wa l d a p a t d i c e g a hd e n g a np e mb e r i a n 9 a n t i ok s i d a n . F l a v on oi d me r u p a k a n s a l a h s a t u b e n t u k a n t i ok s i d a n .S a l a hs a t u a v on oi dy a n gp e n t i n ga d a l a h a n t os i a n i n . Umb iu b ij a l a ru n g u (Ipomoea batatas) me r u p a k a ns a l a hs a t ub a h a n ma k a n a ny a n g me mi l i k i k a n d u n g a ns e n y a wa a n t i ok s i d a ny a n gs a n g a tt i n g g i , y a i t ui s oa v on oi d . Umb iu b ij a l a ru n g ut e l a hb a n y a k d i g u n a k a nu n t u kk oms u ms i s e h a r i h a r i . Ad a n y ak e n y a t a a nb a h wa k a n k e rb i s ad i c e g a h d e n g a na n t i ok s i d a n ,ma k a me mb e r i k a nk e mu n g k i n a n p e n g g u n a a nu mb iu b ij a l a ru n g uy a n g me n g a n d u n g a n t i ok s i d a n u n t u k me n c e g a h k a n k e r . Un t u k me mb u k t i k a ns e c a r ai l mi a ha k t i v i t a su mb iu b ij a l a r u n g u ma k as e l a n j u t n y ap e r l ud i l a k u k a np e n g u j i a n s e c a r al a n g s u n gt e r h a d a ps e l k a n k e r . BAHAN DAN CARA P e n e l i t i a n i n i me r u p a k a n p e n e l i t i a n e k s p e r i me n t a l mu r n id e n g a nr a n c a n g a n post test with c ontrolgroup design. P e n e l i t i a nd i l a k u k a nd i La b or a t or i u mP e n e l i t i a n 26

d a nP e n g u j i a nT e r p a d u (LP P T) Un i v e r s i t a s Ga d j a h Ma d ad e n g a ne k s t r a ke t a n ol u mb i u b i j a l a r u n g us e b a g a i b a h a nu j iu t a ma d a nd ox or u b i c i n es e b a g a ik on t r ol p os i t i f . Awa l n y ad i l a k u k a nd e t e r mi n a s it a n a ma nu mb i u b i j a l a r u n g u , k e mu d i a nd i l a k u k a np e mb u a t a ne k s t r a k e t a n ol u mb i u b i j a l a ru n g u . P e r s i a p a ny a n gd i l a k u k a ns e b e l u mp e me r i k s a a n u j i s i t ot ok s i s i t a sd a na n t i p r ol i f e r a t i fa d a l a hp e mb u a t a n l a r u t a ns t ok b a h a nu j ie k s t r a ke t a n olu mb iu b ij a l a r u n g u ,p e mb u a t a n me d i a RP M I16 40,p e n g a k t i f a ns e l k a n k e rp a y u d a r a T47 D,d a np e mb i a k a ns e lk a n k e r p a y u d a r a T47 D. P e me r i k s a a nu j is i t ot ok s i kp a d as e l k a n k e rp a y u d a r a T47 D me n g g u n a k a n me t od e trypan bl ue ex c l usion assay. Uj ip e n g h a mb a t a n p r ol i f e r a s ie k s t r a k e t a n ol u mb iu b ij a l a ru n g up a d as e lk a n k e rp a y u d a r a T47 D d i l a k u k a nd e n g a n me n g i n k u b a s i k a ns e lk a n k e ry a n g a k a nd i b e r ip e r l a k u a ns e l a ma24 j a mp a d ami c r op l a t e o 96s u mu r a nd i d a l a mi n k u b a t orCO2 5% 37C, d e n g a n j u ml a h s e l 20.000 t i a p t i a p s u mu r a n . Di l a k u k a n p e n g h i t u n g a nj u ml a hs e ls e b e l u m d i b e r ip e r l a k u k a n a t a up a d aj a m k e 0. S e l a n j u t n y ad i b e r i k a nb a h a nu j i d e n g a nv a r i a s i t i g ak on s e n t r a s i .S e b a g a i k on t r ol n e g a t i f d i g u n a k a ns e lk a n k e ry a n gd i b e r ime d i a RP M I16 40 t a n p ap e r l a k u a n . Un t u kt i a pk on s e n t r a s id i l a k u k a n t i g au l a n g a n (t r i p l i k a t ). S e lk a n k e ry a n gt e l a hd i b e r i p e r l a k u a ni n id i i n k u b a s is e l a ma 24,48,d a n7 2 j a m k e mu d i a nd i h i t u n gp e r t u mb u h a ns e ls e c a r av i s u a lt i a p 24, 48, d a n7 2j a m. P e r s e n t a s e p e n g h a mb a t a n d i h i t u n g d e n g a n p e r s a ma a nb e r i k u t[ (j u ml a hs e lh i d u pp a d ak e l omp ok k on t r ol -j u ml a hs e l h i d u pp a d ak e l omp okp e r l a k u a n )/ j u ml a hs e lh i d u pp a d ak e l omp ok k on t r ol ] x 100%. P e r b e d a a n p e r s e n t a s e p e n g h a mb a t a n s e ld i a n t a r a ma s i n g ma s i n gk e l omp okp e r l a k u a nd i u j i s e c a r a s t a t i s t i k d e n g a n me n g g u n a k a nu j i t . J u ml a hp e r t u mb u h a ns e l u n t u kma s i n g ma s i n gk on s e n t r a s i s e t i a pb a h a nu j i d a n k on t r old i t e n t u k a n ,k e mu d i a nd i t e n t u k a np e r s a ma a n l i n i e rs e h i n g g ad i d a p a tn i l a i doubl ing time. Ni l a i doubl ing time d i u j i s e c a r a s t a t i s t i kd e n g a nme n g g u n a k a n u j i An ov ad e n g a nCondenc e Interv al 95%, k e mu d i a n d i l a n j u t k a nd e n g a nT ukeyHS D.
J Peny Dal am, V ol ume 1 1Nomor 1Januari 2 0 1 0

HAS I L 1 . Ek s t r a k Et a n o lUmb iUb iJa l a r Un g u Be r s i f a t Si t o t o k s i kt e r h a d a p Se l Ka n k e rPa y u d a r aT47D d e n g a nme t o d etr y panbl u e ex c l u si onassay . P e n g h i t u n g a ns e ly a n g me n g a l a mi k e ma t i a n d i a ma t is e c a r av i s u a ld e n g a n me t od e trypan bl ue ex c l usion assay. M e t od ei n i me mu n g k i n k a nu n t u k me mb e d a k a na n t a r as e ly a n gh i d u pd e n g a ns e ly a n g

s e ly a n gh i d u pp a d ap e r l a k u a nd e n g a nb a h a nu j id a n s e lh i d u pp a d ak e l omp ok t a n p ap e r l a k u a n . Da t ah a s i l p e n g u k u r a na k t i v i t a ss i t ot ok s i ke k s t r a ke t a n ol u r b i u b i j a l a ru n g up a d as e l k a n k e rp a y u d a r aT47 Dd e n g a n me t od etrypan bl ue ex c l usion assay d a p a t d i l i h a t p a d a T a b e l1 d a n 2. T a b e l1 me n g g a mb a r k a nj u ml a hs e l y a n g me n g a l a mik e ma t i a ns e t e l a hd i b e r i k a ne k s t r a k e t a n ol u mb iu b ij a l a ru n g ud i b a n d i n g k a nd e n g a n k on t r ol n e g a t i f .

Ga mb a r 1. M or f ol og i s e l k a n k e r p a y u d a r aT47 Dd e n g a np e wa r n a a nme n g g u n a k a nb i r ut r i p a n . Mi k r os k opb i n ok u l e r p e mb e s a r a n 10X. S e l h i d u pt a mp a kb e r s i n a rd a nt r a n s p a r a n(t a n d ap a n a hb i r u )s e d a n g k a ns e l ma t i b e r wa r n ah i t a m (t a n d ap a n a hh i t a m).

ma t ik a r e n atrypan bl ue a d a l a hz a twa r n ay a n gh a n y a b i s a me n e mb u s me mb r a ns e ly a n g ma t i . S e ly a n g h i d u p(v iabel )a k a nt a mp a kme mb u l a t , b e r wa r n ap u t i h me n g k i l a t ,d a nb e r s i n a r . S e ly a n g ma t i(non v iabel ) a k a nb e r wa r n ab i r ut u ad a nu k u r a n n y al e b i hb e s a r d a r i p a d as e l h i d u p . Ga mb a r a ns e l y a n gh i d u pd a nma t i s e t e l a h me n d a p a te k s t r a ke t a n olu mb iu b ij a l a ru n g u d a p a t d i l i h a t p a d aGa mb a r1. Ga mb a r 1 me r u p a k a ng a mb a r s e l k a n k e r p a y u d a r a T47 Ds e t e l a hd i b e r i k a nb a h a nu j i y a k n i e k s t r a ke t a n ol u mb iu b ij a l a ru n g ud a ny a n gt i d a kd i b e r i k a ne k s t r a k e t a n ol u mb i u b i j a l a r u n g us e t e l a hd i b e r i k a nb i r ut r i p a n . P a d ap e n e l i t i a ni n ima s i n g ma s i n gs u b y e kd i l a k u k a n r e p l i k a s i t i g a k a l i d a nk e mu d i a nd i l a k u k a np e n g h i t u n g a n j u ml a hs e lk a n k e rs e r v i ky a n gh i d u pu n t u kt i a p t i a p k e l omp ok .P e r s e n t a s ep e n g h a mb a t a np e r t u mb u h a ns e l k a n k e rs e r v i kS i Ha d i d a p a td e n g a n me mb a n d i n g k a n

T a b e l1. Ak t i v i t a ss i t ot ok s i ke k s t r a ke t a n olu b ij a l a ru n g u p a d as e l k a n k e rp a y u d a r aT47 D

J u ml a hs e l y a n gme n g a l a mi k e ma t i a n Dos i s S e n y a wa Re p l i k a s i I Re p l i k a s i I I Re p l i k a s i I I I Ra t a r a t a Uj i s e l y a n g (g / mL) ma t i 500 5000 5.500 5.000 5.16 6 , 6 6 1.000 2.000 3.000 Ek s t r a k 4.000 Ub i J a l a r 5.000 Un g u 6 .000 7 .000 8.000 9.000 10.000 Kon t r ol n e g a t i f 8.000 10.500 12.500 13.500 15.000 16 .000 16 .500 17 .500 20.500 20.000 37 .000 8.500 10.500 12.500 13.500 15.000 16 .000 16 .500 17 .500 20.000 20.000 37 .000 8.000 10.500 12.500 13.500 15.500 16 .000 16 .500 17 .500 20.500 20.000 37 .000 8.16 6 , 6 7 10.500, 00 12.500, 00 13.500, 00 15.16 6 , 6 6 16 .000, 00 16 .500, 00 17 .500, 00 20.333, 33 20.000, 00 37 .000, 00

Ef e kS i t ot ok s i kd a nAn t r ol i f e r a t i fEk s t r a kEt a n ol Umb i Ub i J a l a rUn g u(Ipomoea batatas L) T e r h a d a pS e l Li n eKa n k e rP a y u d a r aT47 D I Wayan Sumardika, Agung Wiwiek Indrayani, I Made Jawi, Dewa Ngurah Suprapta, Losen Adnyana

27

T a b e l2 me n g g a mb a r k a nj u ml a hs e ly a n g me n g a l a mi k e ma t i a ns e t e l a hd i b e r i k a nd ox or u b i c i n e .
T a b e l2. Re r a t aj u ml a hs e lk a n k e rp a y u d a r a T47 D y a n g h i d u pd a np e r s e n t a s ep e n g h a mb a t a np e r t u mb u h a ns e l k a n k e r p a y u d a r a T47 D s e t e l a hp e r l a k u a nd e n g a ne k s t r a ke t a n ol u mb i u b i j a l a ru n g u
Ba h a nUj i Ko n s e n t r a s i Ju ml a hSe l Ha mb a t a n (g / mL) Ka n k e rHi d u p (% SD) 10.000 9.000 8.000 7 .000 6 .000 5.000 4.000 3.000 2.000 1.000 500 120 6 0 30 15 7 , 5 3, 7 5 1, 87 5 0, 9357 5 0, 46 87 5 0 0 0 2.833, 33 4.6 6 6 , 6 7 5.500, 00 7 .6 6 6 , 6 7 10.333, 33 12.500, 00 15.833, 33 21.500, 00 27 .16 6 , 6 7 0 0 1.333 3.333 5.6 6 7 6 .900 10.000 13.950 17 .833 37 .500 100 100 92, 44 87 , 56 85, 33 7 9, 56 7 2, 44 6 6 , 6 7 57 , 7 8 42, 6 7 27 , 56 100 100 96 , 44 91, 11 84, 89 81, 6 0 7 3, 33 6 2, 80 52, 45 0

e t a n ol u mb i u b i j a l a ru n g u . Ha l s e r u p ad i t e mu k a np u l a p a d ap e r s e n t a s ep e n g h a mb a t a np e r t u mb u h a ns e l k a n k e r y a n g me n u n j u k k a np e r b e d a a ny a n gb e r ma k n ad e n g a n me n g g u n a k a nu j i An ov a(p< 0, 05). Dib a wa hi n ime r u p a k a ng a mb a r a ns e lk on t r ol d a ns e ly a n gt e l a hd i b e r i k a ne k s t r a ke t a n olu mb iu b i j a l a ru n g u . T e r l i h a ts e ld e n g a nk on s e n t r a s ie k s t r a k e t a n ol u mb i u b i j a l a r u n g uk on s e n t r a s i t e r e n d a hs a mp a i t e r t i n g g i d a p a t d i l i h a t p a d aGa mb a r2 d a n3.

Ek s t r a kEt a n ol Umb i Ub i J a l a r Un g u

Dok s or u b i s i n

Ga mb a r2. S e l T47 D Kon t r ol

Kon t r oln e g a t i f

P a d aT a b e l1 d ia t a st e r l i h a ts e c a r au mu m p e n i n g k a t a nk on s e n t r a s ib a h a nu j ia k a n me n y e b a b k a n p e n i n g k a t a n p e r s e n t a s e k e ma t i a n s e lk a n k e r . P a d a a n a l i s i ss t a t i s t i k me n g g u n a k a nu j iAn ov a (p < 0, 05) u n t u kme n g e t a h u i j u ml a hs e l y a n gme n g a l a mi k e ma t i a n , t e r n y a t ad i t e mu k a np e r b e d a a ny a n gb e r ma k n ap a d a ma s i n g ma s i n gk on s e n t r a s i e k s t r a ke t a n ol u mb i u b i j a l a r u n g u . P a d aT a b e l2 t e r l i h a tb a h wa me n i n g k a t n y a k on s e n t r a s i e k s t r a ke t a n ol u mb i u b i j a l a ru n g u , j u ml a h s e lk a n k e rp a y u d a r a T47 D y a n g h i d u p me n u r u n y a n gd i s e r t a ip e n i n g k a t a np e r s e n t a s ep e n g h a mb a t a n p e r t u mb u h a n s e l k a n k e r . P a d a a n a l i s i s s t a t i s t i k me n g g u n a k a nu j i An ov a(p < 0, 05) u n t u k me n g e t a h u i j u ml a hs e ly a n gh i d u pd i t e mu k a np e r b e d a a ny a n g b e r ma k n ap a d a ma s i n g ma s i n gk on s e n t r a s ie k s t r a k

Ga mb a r 3. S e l T47 D +500 g / mLEk s t r a kEt a n ol Umb i Ub i J a l a rUn g u

Ga mb a rb e r i k u t i n i me n u n j u k k a nmor f ol og i s e l k a n k e r p a y u d a r aT47 Dd i l i h a td i b a wa h inv erted mic rosc ope. T e r l i h a t s e l d e n g a nk on s e n t r a s i e k s t r a ke t a n ol u mb i u b i j a l a ru n g uk on s e n t r a s i t e r e n d a hs a mp a i t e r t i n g g i d a p a t d i l i h a t p a d ag a mb a r4 s a mp a i 13.

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Ga mb a r4. S e lT47 D + 1.000 g / mL Ek s t r a k Et a n olUmb i Ub i J a l a rUn g u

Ga mb a r7 .S e lT47 D + 4.000 g / mL Ek s t r a k Et a n olUmb i Ub i J a l a rUn g u

Ga mb a r5. S e lT47 D + 2.000 g / mL Ek s t r a k Et a n olUmb i Ub i J a l a rUn g u

Ga mb a r8. S e lT47 D + 5.000 g / mL Ek s t r a k Et a n olUmb i Ub i J a l a rUn g u

Ga mb a r6 .S e lT47 D + 3.000 g / mL Ek s t r a k Et a n olUmb i Ub i J a l a rUn g u

Ga mb a r9. S e lT47 D +6 .000 g / mL Ek s t r a k Et a n olUmb i Ub i J a l a rUn g u

Ef e kS i t ot ok s i kd a nAn t r ol i f e r a t i fEk s t r a kEt a n ol Umb i Ub i J a l a rUn g u(Ipomoea batatas L) T e r h a d a pS e l Li n eKa n k e rP a y u d a r aT47 D I Wayan Sumardika, Agung Wiwiek Indrayani, I Made Jawi, Dewa Ngurah Suprapta, Losen Adnyana

29

Ga mb a r10. S e l T47 D +7 .000 g / mL Ek s t r a kEt a n ol Umb i Ub i J a l a rUn g u

Ga mb a r 13. S e l T47 D +10.000 g / mL Ek s t r a kEt a n ol Umb i Ub i J a l a rUn g u

Ga mb a r11. S e l T47 D + 8.000 g / mL Ek s t r a kEt a n ol Umb i Ub i J a l a rUn g u

2 . Ek s t r a k e t a n o lu b ij a l a ru n g u me n g h a mb a t p r o l i f e r a s i s e l k a n k e rp a y u d a r aT47D Un t u k me n i l a ia p a k a he k s t r a ke t a n olu mb iu b i j a l a r u n g ume mi l i k i a k t i v i t a sp e n g h a mb a t a np r ol i f e r a s i s e lma k ad i b e r i k a np e r l a k u a ny a n gs a ma d e n g a nu j i s i t ot ok s i k . Ba h a nu j id i g u n a k a nt i g ak on s e n t r a s iy a i t u 500, 1.000 d a n 2.000 g / ml .P e n g h i t u n g a nj u ml a hs e l k a n k e rp a y u d a r aT47 Dj u g ame n g g u n a k a ntripan bl ue s e p e r t i h a l n y ap a d au j i s i t ot ok s i k .J u ml a hs e l p a d ak u l t u r s e lk a n k e rd i h i t u n gs e c a r av i s u a ls e t e l a hd i i n k u b a s i d e n g a nb a h a nu j is e l a ma0,24,48,d a n7 2j a m. Da t a l e n g k a pp e r t u mb u h a ns e lk a n k e r s e t e l a hp e r l a k u a n d e n g a nb a h a nu j i u n t u kma s i n g ma s i n gma s ai n k u b a s i d i t a mp i l k a np a d at a b e l 3s a mp a i 5.
T a b e l3. Ha s i lp e r t u mb u h a ns e lk a n k e rp a y u d a r a T47 D s e t e l a hp e mb e r i a ne k s t r a ke t a n olu mb iu b ij a l a ru n g u ma s a i n k u b a s i 24 j a m
Kon s e n t r a s i J u ml a hS e l Ka n k e rP a y u d a r aT47 D Hi d u p(104) (g / ml ) Kon t r ol n e g a t i f 500 1.000 2.000 Re p l i k a s i 1 4, 2 3, 6 3, 00 2, 55 Re p l i k a s i 2 4, 25 3, 55 2, 95 2, 55 Re p l i k a s i 3 4, 2 3, 55 3, 0 2.55

Ga mb a r12. S e l T47 D + 9.000 g / mL Ek s t r a kEt a n ol Umb i Ub i J a l a rUn g u

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T a b e l 4. Ha s i l p e r t u mb u h a ns e l k a n k e r p a y u d a r aT47 Ds e t e l a h p e mb e r i a ne k s t r a ke t a n ol u mb i u b i j a l a ru n g uma s ai n k u b a s i 48 j a m
Kon s e n t r a s i J u ml a hS e l Ka n k e rP a y u d a r aT47 D Hi d u p(104) (g / ml ) Kon t r ol n e g a t i f 500 1.000 2.000 Re p l i k a s i 1 8, 50 7 , 25 6 , 00 5, 30 Re p l i k a s i 2 8, 0 7 , 25 6 , 00 5, 25 Re p l i k a s i 3 8, 50 7 , 25 6 , 10 5, 25

T a b e l 5. Ha s i l p e r t u mb u h a ns e l k a n k e r p a y u d a r aT47 Ds e t e l a h p e mb e r i a ne k s t r a ke t a n ol u mb i u b i j a l a ru n g uma s ai n k u b a s i 7 2j a m
Kon s e n t r a s i J u ml a hS e l Ka n k e rP a y u d a r aT47 D Hi d u p(104) (g / ml ) Kon t r ol n e g a t i f 500 1.000 2.000 Re p l i k a s i 1 17 , 1 14, 9 12, 25 10, 7 5 Re p l i k a s i 2 17 , 15 15, 00 12, 30 10, 7 0 Re p l i k a s i 3 17 , 1 14, 95 12, 25 ] 10, 7 5

P EM BAHAS AN P a d ap e n e l i t i a ni n it e l a hd i l a k u k a nu j ie f e k a n t i k a n k e r(s i t ot ok s i kd a na n t i p r ol i f e r a t i f )d a r i e k s t r a k e t a n ol u mb i u b i j a l a r u n g ut e r h a d a ps e l k a n k e r p a y u d a r a T47 D. Umb iu b ij a l a ru n g u me n g a n d u n ga n t os i a n i n y a n gt i n g g i y a k n i 110 mg / 100 g r a ms a mp a i 210 mg / 100 g r a m u mb is e g a r .11 An t os i a n i n me r u p a k a ns a l a hs a t u a n t i ok s i d a ny a n gd i p e r k i r a k a nme mp u n y a i me k a n i s me a n t i k a n k e r . Ha s i lp e n e l i t i a ni n ime n u n j u k k a nb a h wa e k s t r a ke t a n olu mb iu b ij a l a ru n g u me mi l i k ia k t i v i t a s s i t ot ok s i kt e r h a d a ps e lk a n k e rp a y u d a r a T47 D. Ef e k s i t ot ok s i ki n id a p a td i s e b a b k a nk a r e n ak a n d u n g a n s e n y a waa k t i f p a d au mb i u b i j a l a r u n g uy a i t ua n t os i a n i n . P a d ap e n e l i t i a ni n ij u g at e r l i h a tb a h wae k s t r a ke t a n ol u mb i u b i j a l a r u n g ume n g h a mb a t p r ol i f e r a s i s e l k a n k e r p a y u d a r aT47 D. P a d ap r ol i f e r a s is e l ma ma l i a ,r e p l i k a s ig e n d a p a td i b a g ime n j a d i4 f a s es i k l u ss e l .F a s ep e r t a ma d i s e b u tG1 (g a p 1) d i ma n as e lme n g a l a mip e r u b a h a n b i ok i mi au n t u kme n y i a p k a nd i r i me ma s u k i f a s eS(f a s e d i ma n a DNA b a r ua k a nd i s i n t e s a ). P a d af a s eS s e l a k a n me mb u a ts a l i n a n ma t e r i a lg e n e t i ky a n gl e n g k a p

d a nk e mu d i a n me n y i a p k a nf a s ep e r s i a p a nb e r i k u t n y a y a i t u G2 s e b e l u m me ma s u k if a s eM (mi t os i s ). S e l a ma mi t os i ss u a t us e ly a n gb e r p r ol i f e r a s ia k a nb e r s i a pd i r i ma s u kf a s eG1 u n t u kme n y i a p k a nr e p l i k a s i l e b i hj a u h . Ol e hk a r e n ar e p l i k a s is e li n ime r u p a k a np r os e sy a n g r u mi td a ni r e v e r s i b e l ,ma k as e le u k a r i otme l i b a t k a n b e b e r a p ac hec kpoints. M e l a l u il a n g k a hi n i ma k as e l n or ma l d a p a t me ma s t i k a ns i n t e s i s DNA t i d a kt e r i n i s i a s i a t a ut i d a kd i l a n j u t k a nb i l ab e r a d ap a d ak on d i s iy a n g t i d a kd i i n g i n k a ns e r t ak r omos om t i d a kb e r k on d e n s a s i d a n me mi s a h k a nd i r is a mp a ig e ns i a pu n t u kr e p l i k a s i s e l a n j u t n y a d a n me l a k u k a n mi t os i s . Ke h i l a n g a n k on t r ol c hec kpoints i n i a k a nme mb a wae f e ky a n gt i d a k d i i n g i n k a ny a i t ua d a n y av a r i a s i a b n or ma l i t a sp a d ag e n 12 t e r u t a may a n gt e r j a d i p a d as e l k a n k e r . S e ly a n g me n g a l a mi p r ol i f e r a s i me mi l i k i p i l i h a nf a s ey a i t uG0. M a s u kk ef a s eG0 d a r i G1 a d a l a h p i l i h a np e n t i n gp a d ap e r k e mb a n g a ns e l .S e c a r ain v itro d i p e r l u k a n growth f ac tor y a n ga d e k u a tu n t u kd a p a t ma s u kd a r i f a s eG1 k eS .T i t i ki n i d i s e b u t j u g arestric tion point. Bi l al i n g k u n g a ns e l u l e rt i d a kme n c u k u p iu n t u k me l e wa t i restric tion point ma k aa k a nk e mb a l ik e 13 f a s eG0.12, S a l a hs a t ua b n or ma l i t a sphenotypicy a n gs e r i n g p a t og n omon i sp a d as e mu as e l k a n k e r a d a l a ht e r j a d i n y a d i s r e g u l a s ip a d ak on t r ols i k l u ss e ld i ma n as e lk a n k e r 14 a k a nb e r e p l i k a s il e b i hc e p a td i b a n d i n gs e ln or ma l . T i mb u l n y ap e r t u mb u h a n y a n g t i d a k n or ma lp a d a k a n k e rd i s e b a b k a nk a r e n ad u af a k t ory a i t uk u r a n g n y a r e s p on sk on t r olt e r h a d a ps i g n a ly a n gb i a s a n y as e c a r a n or ma lme n y e b a b k a n s e la k a nb e r h e n t iu n t u kt i d a k me l a n j u t k a n s i k l u s s e ld a n k u r a n g n y a p r og r a m k e ma t i a ns e ly a n gb e r e s p on st e r h a d a ps t i mu l u sy a n g 16 T r a n s f or ma s id a r is e b u a hs e l s e s u a ia t a us t r e s .15, n or ma lme n j a d is e lt u mort a mp a k n y at e r g a n t u n gp a d a mu t a s ip a d ap r od u kg e ny a n gp e n t i n gd i d a l a ms i g n a l i n t e g r a s ie k s t r a s e l u l a rd a ni n t r a s e l u l a rt e r h a d a ps i k l u s s e ld a n me s i nk e ma t i a ns e ld a np a d as e mu ap r od u k g e ny a n gt e r l i b a t s e c a r al a n g s u n gme n g on t r ol p r og r e s i s i k l u ss e l . Ke h i l a n g a nb e b e r a p af u n g s it e r s e b u ta k a n me n y e b a b k a nk e h i l a n g a ns i s t e m p e n g a t u r a ns i g n a l 13 p e r t u mb u h a ns e l . 31

Ef e kS i t ot ok s i kd a nAn t r ol i f e r a t i fEk s t r a kEt a n ol Umb i Ub i J a l a rUn g u(Ipomoea batatas L) T e r h a d a pS e l Li n eKa n k e rP a y u d a r aT47 D I Wayan Sumardika, Agung Wiwiek Indrayani, I Made Jawi, Dewa Ngurah Suprapta, Losen Adnyana

Umb iu b ij a l a ru n g ut e l a hd i g u n a k a ns e c a r a e mp i r i s ol e h ma s y a r a k a tu n t u k me n g a t a s ib e r b a g a i p e n y a k i t ,t e r ma s u kk a n k e r .P a d ap e n e l i t i a ni n i ,d a p a t d i b u k t i k a ns e c a r ain v itro b a h wae k s t r a ke t a n olu mb i u b i j a l a ru n g ud a p a t me n i mb u l k a ne f e ks i t ot ok s i kd a n a n t i p r ol i f e r a s i p a d as e l k a n k e r p a y u d a r aT47 D. Na mu n p e r l up e n e l i t i a nl e b i hl a n j u t b a i ks e c a r ain v iv od a nu j i k l i n i ks e b a g a il a n d a s a np e n g g u n a a nu mb iu b ij a l a r u n g us e b a g a i a n t i k a n k e r . KES I MP ULAN Be r d a s a r k a nh a s i l p e n e l i t i a n , d a p a t d i s i mp u l k a n e k s t r a ke t a n ol u mb iu b ij a l a ru n g u me mi l i k ie f e k s i t ot ok s i kt e r h a d a ps e lk a n k e rp a y u d a r aT47 D. Ek s t r a k e t a n olu mb iu b ij a l a ru n g uj u g ad a p a tme n g h a mb a t p r ol i f e r a s i s e l k a n k e rp a y u d a r aT47 Ds e c a r ain v itro. P e r l ud i l a k u k a np e n e l i t i a nl e b i hl a n j u t me n g e n a i p e n g a r u hp e mb e r i a ne k s t r a ke t a n ol u mb i u b i j a l a r u n g u t e r h a d a ps e lk a n k e rs e c a r ain v iv od a nu j ik l i n i ka g a r d a p a td i g u n a k a ns e b a g a il a n d a s a np e n g g u n a a nu mb i u b ij a l a ru n g us e b a g a ia n t i k a n k e r .P a d ap e n e l i t i a ni n i d i g u n a k a ne k s t r a ke t a n olu mb iu b ij a l a ru n g uy a n g me n g a n d u n gb e r b a g a is e n y a waa k t i f . Ol e hk a r e n ai t u p e r l ud i l a k u k a ni s ol a s is e n y a wa a k t i fy a n gb e r p e r a n s e b a g a i a n t i k a n k e r . DAF T AR RUJ UKAN 1. 2. 3. Ki n g RJ B. Ca n c e rb i ol og y . 2nd e d . En g l a n d : P e a r s onEd u c a t i onLi mi t e d ; 2002. h Tj a yTH d a nRa h a r d j aK. Ob a t ob a t p e n t i n g . 5t e d .J a k a r t a : P T . El e xM e d i aKomp u t i n d o; 2002. Da l i ma r t h a S . Ra mu a n t r a d i s i on a l u n t u k p e n g ob a t a nk a n k e r .J a k a r t a :P e n e b a rS wa d a y a ; 2002. Tj i n d a r b u mi Da n dM a n g u n k u s omo R. Ca n c e r i n I n d on e s i a , p r e s e n t , a n df u t u r e .J p nJCl i nOn c ol 2002; 32(1); S 17 S 21. Ca n t o MT , An d e r s on W E, a n d Br a wl e y O.

6 .

7 .

8.

9. 10. 11.

12.

13. 14.

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4.

16 .

5.

Ge og r a p h i cv a r i a t i on i nb r e a s tc a n c e rmor t a l i t y f or wh i t ea n db l a c k wome n . Ca n c e r J Cl i n 2001; 51(8); 36 7 7 0. Kr a me rR a n d Br own P . S h ou l dt a mox i f e nb e u s e di nb r e a s tc a n c e rp r e v e n t i on ? Dr u gS a f e t y 2004; 27 (13); 97 989. Wi l c k e n H, Hor n b oc k l e J ,a n d Gh e r s i D. Ch e mot h e r a p ya l on ev e r s u se n d oc r i n et h e r a p y a l on ef or me t a s t a t i cb r e a s t c a n c e r . Th eCoc h r a n e Col l a b or a t i on2005; 16 . An on i m. Ef f e c t i v eh e a l t hc a r e , t h ema n a g e me n t ofp r i ma r yb r e a s t c a n c e r .I S S N 1996 ; 2(6 ); 0288096 5. Ba k t aI M . An t i ok s i d a nd a nk a n k e r .S i mp os i u m An t i ok s i d a nI DI . Ba l i : I DIBa l i ; 6M a r e t 2004. You n g LS a n d Wood s i d eJ V. An t i ox i d a n t si n h e a l t ha n dd i s e a s e . JCl i nP a t h ol 2001; 54; 17 6 86 . S u p r a p t a DN,d k k . Ka j i a na s p e kp e mb i b i t a n , b u d i d a y a d a n p e ma n f a a t a n u mb i u mb i a n s e b a g a i s u mb e rp a n g a na l t e r n a t i f . La p or a nh a s i l p e n e l i t i a n . Ke r j a s a ma BAP EDA P r op i n s iBa l i d e n g a nF a k u l t a sP e r t a n i a nUNUD; 2004. Ka s t a nM B a n dS k a p e kS X. M ol e c u l a rb i ol og y ofc a n c e r .I n : De Vi t aVT , He l l ma nS , Ros e n b e r g S A,e d i t or s . Th ec e l lc y c l ei nc a n c e rp r i n c i p l e t h & p r a c t i c e of on c ol og y . 6 e d . P h i l a d e l p h i a : Li p p i c ot t Wi l l i a ms&W i l k i n s ; 2001.p .91102. S u k a r d j a I DG. On k ol og ik l i n i k . S u r a b a y a : Ai r l a n g g aUn i v e r s i t yP r e s s ; 2000. S i s mi n d a r i . Cy t ot ox i c e f f e c t s of me t h a n ol e x t r a c t i s ol a t e df r om e r y t h r i n af u s c al ou rl e a v e s onc a n c e rc e l l l i n e s . BIKe d2003; 35(2); 58. As wi n S . Bi ol og i mol e k u l e rk e ma t i a n s e l t e r p r og r a m. P e r t e mu a nI l mi a hT a h u n a nI l mu P e n y a k i tDa l a m. Yog y a k a r t a :F K UGM ;1314 S e p t e mb e r2002. Ut or o T . Ke ma t i a ns e lt e r p r og r a m (a p op t os i s ). P e r t e mu a n I l mi a h T a h u n a n I l mu P e n y a k i t Da l a m. Yog y a k a r t a : F K UGM ; 1314 S e p t e mb e r 2002.

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