INDIAN REFERENCES OF GENETIC TRANSFORMATION IN ORNAMENTALS: Crop Method Tissue used Trait gene Selection marker nptII Vector

used Reference

Gerbera hybrida

Agrobacterium Petiole, leaf -mediated and shoot tip

-glucuronidase (uidA)

Agrobacterium tumefaciens strain LBA4404.

Nagarajun et al.

Carnation

-do-

leaf tissue protocorm

cry1Ab gus A reported gene

kanamycin -do-

LBA4404,EHA105

Kumar et al. Swarnapiria et al. Lakshman et al.

Dendrobium -do-

Gladiolus

-do-

root

hygromycin B enhanced phosphotransferase green fluorescence protein gene (EGFP)

Agrobacterium tumefaciens strain AGL-1

Other worldwide references of genetic transformation in ornamentals:

Abbreviation: Ace-AMP1 = allium antimicrobial protein 1 ACO = ACC oxidase Act1 = rice actin antisense CHS = chalcone synthase att = atticin boers = ethylene receptor from Brassica CER6 = eceriferum promoter CHR = chalcone reductase chs = petunia chalcone synthase chs-A = chalcone synthase promoter EPF2-5 = zinc finger transcription factor etr1-1 = insensitivity to ethylene F3,5H = flavonoid 35-hydroxylase from Vinca major f3h = flavanone 3-hydroxylase fbp1 = flower specific promoter FBP1 = flower-specific petunia promoter GFP = green fluorescent protein ipt = isopentenyl transferase ipt = isopentenyl transferase Lc = maize flavonoid regulation Lhca3.St.1 = promoter from potato MAS = mannopine synthase promoter pin-2 = wound inducible PMC = multicystatin RCC2 = rice chitinase RCH10 = rice chitinase rolA B C = from Agrobacterium rhizogenes SAG12 = senescens-associated transcriptional promoter TSWV = nucleoprotein of tomato spotted wilt tospovirus ubi1 = ubiquitin 1 UBQ10 = ubiquitin from arabidopsis UBQ3 = arabidopsis ubiquitin UBQ3 = ubiquitin from arabidopsis UEP1 = ubiquitin extension protein promoter

Reference: Brand, Mark H., Ornamental Plant Transformation., Journal of Crop Improvement Vol. 17, No. 1/2 (#33/34), 2006, pp. 27-50