DAFTAR ISI

Halaman
LEMBAR PENGESAHAN ........................................................................... ii
KATA PENGANTAR.................................................................................... iii
DAFTAR ISI................................................................................................... iv
DAFTAR TABEL........................................................................................... v
DAFTAR GAMBAR...................................................................................... vi
DAFTAR LAMPIRAN................................................................................... vii
BAB I PENDAHULUAN
1.1

Latar Belakang.......................................................................... 1

1.2

Tujuan Penelitian...................................................................... 2

1.3

Hipotesis................................................................................... 2

BAB II TINJAUAN PUSTAKA

2.1

Deskripsi Tanaman (Melastoma malabathricum Auct. non L) 3

2.1.1 Sistematika Tanaman ...................................................... 4
2.1.2 Sinonim Tanaman ........................................................... 4
2.1.3 Nama Daerah .................................................................. 4
2.1.4 Penyebaran....................................................................... 4
2.1.5 Kandungan Kimia............................................................ 5
2.1.6 Kegunaan Tanaman......................................................... 5

2.2

Radikal Bebas........................................................................... 5
2.2.1 Sifat-sifat Radikal bebas.................................................. 5

2.3

Antioksidan............................................................................... 6
2.3.1 Mekanisme Antioksidan.................................................. 7
2.3.2 Penggolongan Antioksidan ............................................. 8

2.4

Metode Pengujian Aktivitas Antioksidan................................. 9

2.4.1 Metode 1,1-difenil-2-pikrilhidrazil (DPPH).................... 9
2.4.2 Metode Ferri Tiosianat-Linoleat (FTC)........................... 10
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2.5. Asam Askorbat.......................................................................... 12

2.6

Metode Ekstraksi......................................................................12

2.7

Spektrofotometri UV-Vis..........................................................13

BAB IIIBAHAN DAN METODE

3.1

Waktu dan Tempat Penelitian...................................................

3.2 Bahan dan Alat Penelitian............................................................

3.3 Metode Penelitian........................................................................
3.3.1 Determinasi Tanaman .....................................................
3.3.2...........................................................................................
3.3.3...........................................................................................
3.3.4 Pembuatan Ekstrak...........................................................
3.3.5 Rendemen Ekstrak...........................................................
3.3.6...........................................................................................
3.3.7...........................................................................................
3.3.8 Rancangan Percobaan......................................................
DAFTAR PUSTAKA .....................................................................................
LAMPIRAN ...................................................................................................

DAFTAR TABEL

Tabel

Halaman

1.

Nilai IC50 dan sifat keaktifan senyawa antioksidan..............................6

2.

Penggolongan antioksidan....................................................................8

DAFTAR GAMBAR

Gambar

Halaman

1. Daun Harendong (Melastoma malabathricum Auct.nonL).................. 4

2. Mekanisme Inisiasi............................................................................... 7
3. Mekanisme Propagasi........................................................................... 7
4. Mekanisme Terminasi........................................................................... 8
5. Struktur Kimia DPPH........................................................................... 9
6. Mekanisme Penghambatan Radikal DPPH........................................... 10
7. Reaksi Oksidasi asam lemak................................................................. 11
8. Reaksi pembentukan kompleks Feri sianida......................................... 11
9. Struktur Vitamin C................................................................................ 12

10. Grafik Hubungan antara absorbansi dengan konsentrasi...................... 14

11. Bagan Spektrofotometri........................................................................ 15

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