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Sieve through 300 micron sieve

100 gms+100 ml water

Shake flask in a flask shaker for about an hour

Filter through no.1 whatman filter paper

25 ml of the extract in a conical flask

Add one drop of phenolphthelein indicator

Sieve through 300 micron sieve

25 ml of sample

Shake flask in a flask shaker for about an hour

Filter through no.1 whatman filter paper

25 ml of the extract in a conical flask

Add one drop of phenolphthelein indicator

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