QP422 Lista2(MtodosdeIonizao)

1) Considerando o espectro apresentado e o mtodo de ionizao

informado,forneaamassadocompostoanalisado(neutro).

ESI(+)

765.95

100

766.45

766.95
767.46

0
765

766

767.96

767

mass
769

768

EI(+)

170.01

100

172.01
171.01
0
168

169

170

171

172

173

mass
175

ESI()

556.47

100

174

556.22

556.72
556.97
557.22
0

556

557

557.47
mass
558

MALDI(+)

1315.44

100

1316.44
1317.45
1318.45
0
1314

1315

1316

1317

1318

mass
1320

1319

CI(+)

609.28

100

610.28
611.29

0
608

609

610

611

mass
613

612

ESI()

473.85

100

474.18
474.51
474.85
0
473

474

mass
476

475

336.09

EI(+)

338.09

100

475.18

337.09
0
333

334

335

336

337

339.09
338

339

340

341

342

mass
343

APCI(+)

597.51

100

598.51

599.52
0
595

596

597

598

599

600

APCI()

873.50

100

mass
602

601

874.50

875.51
876.51
0
872

873

874

875

876

mass
878

877

2) Calcule a massa das protenas que fornecem os seguintes espectros de

ESI(+)MS.Considereapenasossinaismaisintensosdecadaestadodecarga.

771.4 808.1 848.5

100

893.1

942.7

998.1

707.2

1060.4

1131.0
1211.8
679.0
977.0

700

800

900

1000

1100

1200

m/z

100

727.9

773.4 824.9
883.7
951.6

687.5

1030.8

1236.9

1124.5
651.4

0
600

700

800

900

1000

1432.2

100

1100

1200

m/z

1595.4

1302.1
1193.6

1804.1
1102.0

0
1000

1100

1200

1300

1400

1500

1600

1700

1800

1900

m/z

QP422GabaritodaLista2

Questo1

A)1529,88Da
B)170,01Da
C)2228,93Da
D)1314,43Da
E)608,27Da
F)1424,53Da
G)336,09Da
H)596,50Da
I)874,51Da

Questo2

A)16970Da
B)12370Da
C)14322Da