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Spectrophotometric Determination of the Acid Dissociation Constant of Methyl Red

J.P. Loja Institute of Chemistry, University of the Philippines, Diliman, Quezon City September 3, 2013 September 11, 2013 I. Methodology Volume of Standard Methyl Red (mL) 6.00 6.00 6.00 6.00 Volume of 0.0200 M HOAc (mL) 1.20 2.40 4.80 7.20 Volume of 0.040 M NaOAc (mL) 12.80 11.60 9.20 6.80

The materials used in the experiment were volumetric flasks, beakers, pH meter and UV-Vis Spectrophotometer. The first part of the experiment was the preparation of solutions. In a 50 mL volumetric flask, methyl red stock solution was prepared by dissolving 0.05 g methyl red in 35 mL 95% ethanol and was diluted to mark. Methyl red standard solution was made in a 50 mL volumetric flask using 2.50 mL of stock mixed with 25 mL 95 % ethanol. Corresponding masses were weighed to make the reagents namely: 100 mL 0.040 M NaOAc through NaOAc3H2O crystals, 50 mL 0.010 M NaOAc from 0.040 M NaOAc solution, 50 mL 1.0 M HOAc, 50 mL 0.020 M HOAc from 1.0 M HOAc , 50 mL 0.10 M HCl, and 50 mL 0.010 M HCl from 0.10 M HCl. These were dissolved in a volumetric flask and diluted to mark. The acidic methyl red (HMR) was prepared in a 50 mL volumetric flask by adding 5.00 mL methyl red standard solution in 5.00 mL 0.100 M HCl solution. The solution was dissolved to mark and the pH measured if it was 2. The alkaline methyl red (MR-) was made in a 50 mL volumetric flask by adding 5.00 mL methyl red standard solution in 12.40 mL 0.040 M NaOAc solution. This was then diluted to mark and the pH measured should be 8. The solutions were prepared as indicated in Table I. Table I. Sample Solution Preparation Sample Volume 0.010 M HCl Vol. HMR Soln Soln (mL) (mL) Number 1 5.0 mL 15.0 mL 2 10.0 mL 10.0 mL 3 15.0 mL 5.0 mL Volume 0.010 M Vol. MR- Soln NaOc (mL) (mL) 4 5.0 mL 15.0 mL 5 10.0 mL 10.0 mL 6 15.0 mL 5.0 mL

7 8 9 10

A 50 mL or 100 mL volumetric flask was used in solutions 1-6 and 100 mL or 150 mL volumetric flask for 1-7. The sample were prepared but was not diluted to mark. The spectra of HMR and MR- solution was obtain between 350 and 600 nm using distilled water for a reference cell. A matched cell was used for all the absorbance measurement. Maximum absorption (max) of wavelengths was determined from the obtained spectra of acidic, HMR, and basic, MR-, forms of methyl red. The absorbance of sample 1-6 both acidic, HMR, and basic, MR-, using distilled water as reference cell was determined. II. Results and Discussion

One of the methods known to determine the absorption of light was spectrophotometry. A complimentary color of the solution was commonly used since different compounds absorb different amount of light. Spectrophotometer is a machine that can determine the absorbance of a solution. It would pass a series of monochromatic light on the substance and a part of it would be absorbed and the rest transmitted. The amount of light absorbed by a solution can be used to compute an unknown concentration of an analyte by getting the absorbance through BeerLamberts Law. The Beer-Lamberts law shows the linear relationship of absorbance and concentration as shown in Equation. 1.[2] A = kbc Equation 1. Beer-Lamberts Law A = absorbance k = proportionality constant

b = path length c = concentration of absorbing species In the experiment, the unknown concentration of a solution was determined by taking readings on each twice in different wavelengths as shown in Table II. Table II. Measured pH and Absorbance for HMR and MRSamples 1 2 3 4 5 6 7 8 9 10 HMR 0.554 0.278 0.185 0.026 0.021 0.011 0.235 0.394 0.775 1.107 MR 0.044 0.023 0.017 0.221 0.149 0.073 0.857 0.802 0.699 0.583 pH 6.5 6.3 5.9 5.6

These wavelengths were made to calibration curves where the absorbance was graphed against concentration to take the linear regression. In the mixture of HMR and MR-, the absorbance at a particular


Conclusion References Appendices