Student Page 1.

1: First Griffith Experiment Griffith observed that the bacteria that caused pneumonia came in two different forms: the R strain formed rough-looking colonies when grown on agar plates. the S strain formed smooth-looking colonies when grown on agar plates. The S strain cells were enclosed by a capsule made of polysaccharide (sugar) while the R strain lacked this capsule. Griffith injected mice with each strain and observed the results.

Observations:

Claim: (Why you think this happens) Evidence and Reasoning: (What makes you think this is the reason)

Student Page 1.2: Second Griffith Experiment Griffith wondered what property of the S strain bacteria was responsible for causing the disease. He killed some S-strain bacteria by heating them and then injected them into mice. The mice lived.

Observations:

Claim: Evidence and Reasoning:

Student Page 1.3A: Third Griffith Experiment In this third and final experiment, Griffith decided to co-inject mice with living R strain bacteria and heat-killed S strain bacteria.

Type of cells injected Living R strain Heat-killed S strain

Experimental outcome

Predicted Living R strain + heat-killed S strain

Actual

Student Page 1.3B: Third Griffith Experiment

Mice that were co-injected with living R strain and heat-killed S strain bacteria died from pneumonia.

Griffith was surprised to see that the co-injection killed the mice. To conclude his experiment, Griffith isolated bacteria from the dead mice and grew them on agar plates. What type of bacteria grew on the agar plates, R strain or S strain? Why do you think so?