GLYCOLYSIS & TCA CYCLE -

ENZYMES & EC REFERENCE NUMBERS

GLUCOSE
ATP
2.7.1.1 Hexokinase ADP
Glucose-6-P
5.3.1.9 Glucose-6-phosphate isomerase
Fructose-6- P ATP
2.7.1.11 6-Phosphofructokinase
ADP
Fructose-1,6-bis-P
4.1.2.13 Fructose-bis-phosphate aldolase
3-P-Triose
2NAD+
1.2.1.12 Glyceraldehyde 3-P-dehydrogenase
NADH+H +
1,3-bis-P-Glycerate
2ADP
2.7.2.3 Phosphoglycerate kinase IUBMB
ATP
3-P-Glycerate ENZYME
5.4.2.1 Phosphoglycerate mutase
2-P-Glycerate
COMMISSION
4.2.1.11 Phosphopyruvate hydratase (EC)
H2 O
P-enolpyruvate ADP
CLASSIFICATION
2.7.1.40 Pyruvate kinase
ATP
NUMBERS
PYRUVATE
1... OXIDOREDUCTASES
2... TRANSFERASES
PYRUVATE 3... HYDROLASES
1.2.4.1 Pyruvate dehydrogenase (lipoamide)
NADH+H+ 4... LYASES
ACETYL-CoA 5... ISOMERASES
Succinyl-CoA hydrolase 4.1.3.7 Citrate synthase
6... LIGASES
Oxaloacetate Citrate
4.2.1.3 Aconitate hydratase
NADH+H+
1.1.1.37 Malate dehydrogenase
Malate Isocitrate
Fumarate 4.2.1.2 1.1.1.41 Isoocitrate dehydrogenase
hydratase H 2O
NADH+H+
Fumarate 2-Oxoglutarate
UQH2
1.3.5.1 Succinate dehydrogenase (ubiquinone)
1.2.4.2 Oxoglutarate dehydrogenase (lipoamide)
Succinate NADH+H+
Succinyl-CoA
GDP
6.2.1.4 Succinate-CoA ligase (GTP-forming)
GTP

ENZYMES
1.1.1.37 Malate dehydrogenase 3.1.2.3 Succinyl-CoA hydrolase
1.1.1.41 Isocitrate dehydrogenase 4.1.2.13 Fructose-bis-phosphate aldolase
1.2.1.12 Glyceraldehyde-3-P dehydrogenase 4.1.3.7 Citrate synthase
1.2.4.1 Pyruvate dehydrogenase (lipoamide) 4.2.1.2 Fumarate hydratase
1.2.4.2 Oxoglutarate dehydrogenase (lipoamide) 4.2.1.3 Aconitate hydratase
1.3.5.1 Succinate dehydrogenase (ubiquinone) 4.2.1.11 Phosphopyruvate hydratase
2.7.1.1 Hexokinase 5.3.1.9 Glucose-6-phosphate isomerase
2.7.1.11 6-Phosphofructokinase 5.4.2.1 Phosphoglycerate mutase
2.7.1.40 Pyruvate kinase 6.2.1.4 Succinate-CoA ligase
2.7.2.3 Phosphoglycerate kinase
The IUBMB Enzyme Commission (EC) Numbers are all 4-figure numbers, the
first of which denotes one of ONLY 6 CLASSES of enzyme as shown above.
These identify the biochemical nature of every reaction and are worth memorising