Synthesis and Characterization of Peptidyl Fluorescent Probe

Dansyl - Glycine -Serine - Glycine - Serine - Methyl Ester

( Dns - Gly - Ser - Gly - Ser - OMe ),Application For Metal Ions Detection
Mohd Rashidi Abdull Manap, Nor Azah Yusof *,
Siti Mariam Mohd Nor, Faujan Hj Ahmad

Department of Chemistry, Faculty of Science

Universiti Putra Malaysia,43400 Serdang,Malaysia
Corresponding email*: azah@science.upm.edu.my

Certain heavy metals can pose potential health risks when consumed by humans due to their high toxicity. Heavy metals such as
mercury, lead and cadmium can be released into the environment through industrial use and erosion of natural deposits and once
released can contaminate water, soil and air(Jarup.,2003).It is important that we have a sensor that can detect those metals.
Some of the peptides that have been applied in metal detection systems were Cys-Pro-Gly-His-Glu (Joshi and Lee., 2008) and
GGDGGDGGDGGDGG (White and Holcombe., 2007) where G is Glycine and D is Aspartic Acid. New peptide for sensor
development that will be synthesized is a combination of Glycine and Serine and combined with fluorophore Dansyl (Dns) at N-
terminal of Glycine. The expected Dns-GSGS will be used to detect metals by monitoring the fluorescence properties.

SYNTHESIS APPLICATION
Boc-Amino Acid
+ Amino Acid-OMe Boc-Dipeptide-OMe

DCC,TEA 4 M HCl in Dioxane Selectivity

Crude
Limit of
Dipeptide-OMe

Column
detection
Chromatography
Dipeptide-OMe
+ Amino Acid-OMe
Boc-Dipeptide-OMe
pH study
Boc-Tripeptide-OMe

A new biosensor Dns-Gly-Ser-Gly-Ser-OMe will be synthesized by

solution phase technique .Then,it will be applied as a biosensor to
detect metal (cation/anion) at optimum parameters such as pH and
ligand concentration .Selectivity,detection limit and interference
towards certain metal also will determin

Jarup,L. (2003) .Hazards of heavy metal contamination. Br Med Bull. 68: pp 167-182.

Joshi,B.P.,Lee,K.-H.(2008).Synthesis of highly selective fluoresecent peptide probes for metal ions:tuning

selective metal monitoring with secondary structure.Bioorganic & Medicinal Chemistry.16:pp8501-8509. Thank you to Ministry of Science, Technology & Innovation,
Malaysia (MOSTI) for financial
White,B.R.,Holcombe,J.A.(2007).Fluorescent peptide sensor for the selective detection of Cu2+.Talanta.71 support under Science Fund 03-01-04-SF0134 (5450218)
:pp 2015–2020.