[S]

(umol/L)
0
8.33
10
12.5
16.7
20
25
33.3
40
50
60
80
100
200

1/[S]

(nmol/L/min) .
0
13.8
16
19
23.6
26.7
30.8
36.3
40
44.4
48
53.4
57.1
66.7

1/V
.

#DIV/0!
0.120048
0.1
0.08
0.05988
0.05
0.04
0.03003
0.025
0.02
0.016667
0.0125
0.01
0.005

1/V = (1/Vmax) + (Km/Vmax) * (1/[S])

#DIV/0!
0.072464
0.0625
0.052632
0.042373
0.037453
0.032468
0.027548
0.025
0.022523
0.020833
0.018727
0.017513
0.014993

0.08
0.07
0.06
0.05
0.04
0.03
0.02
0.01
0
0

intersep
slope

0.05

0.012501
0.499907

Km/Vmax) * (1/[S])

Series1

0.1

Vmax
Km

79.99231
39.98872

0.15

Tentukan Vmax dan Km

V = Vmax * ( [S] / ([S} + Km )
Vmax=
Km=
Actual Data:
[S]
V
(mmol/L) (mmol/L/min)
0.00
0.0
8.33
13.8
10.00
16.0
12.50
19.0
16.70
23.6
20.00
26.7
25.00
30.8
33.30
36.3
40.00
40.0
50.00
44.4
60.00
48.0
80.00
53.4
100.00
57.1
200.00
66.7

mmole/L/min
umole/L