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RESULT From the experiment of washing helminth protein till the activity test against Klebsiella sp.

,we got lysate or helminth protein extract.Then,the result of fractination are twenty fraction that will be measured its absorbance.Three fractions with the highest absorbance,fraction two,six and seven,will be taken to be tested.Next,those three fractions will be diluted until 0,517.The activity test on the second,sixth,and seventh fraction of protein sampel shows no inhibition zone against Klebsiella sp.Below is table of absorbance and diameters of inhibition zone that experimenters got from the experiment : Table of Absorbance No 1 2* 3 4 5 6* 7* 8 9 10 11 12 13 14 15 16 17 18 19 20 Table of diameters inhibition zone Petri Disk Chloramphenicol Extract protein helmint 2,37 cm 260 280

Fraction 2

Fraction 6

Fraction 7

Etanol and water -


2,47 cm

CONCLUSION Based on the test that have been done,we can conclude that Klebsiella sp. susceptibility could be discovered by using disk difusion method and the result shows that only Chloramphenicol gave inhibition zone against Klebsiella sp.Meanwhile,the other substances thats tested didnt give any inhibition zone against Klebsiella sp.