Acta Ichthyologica et Piscatoria

Title: DNA barcoding of freshwater fishes from lake Laut Tawar, Indonesia Authors Zainal A. MUCHLISIN, Nur FADLI, Mohd N. SITI-AZIZAH

MS# 1175

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Does it represent a topic that is widely cited? Do the authors define directions for future research (resulting from their study)? Is the manuscript well organized?

Are length and content in agreement? Is the paper internally consistent? Are there any unnecessary repetitions? Are there any contradictions? Are the conclusions and interpretations based upon the described data? Is there any aspect that might become a source of misunderstanding? Can you suggest any reduction in the paper or deletions of parts? Can you suggest brief additions that will enhance the value of this paper?

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Is the terminology used consistent with widely accepted norms? Do names of new taxa follow the International Code of Zool. Nomenclature.? (where applicable) Does the title adequately represent the content of the paper? required for individual chapters see instructions for authors)? Are all the illustrations and tables necessary and acceptable?

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12. Is the references list complete and coordinated with the text? 13. Is the language of the text correct, clear and unambiguous? Acceptability for publication: - as it is - with minor revision X - after substantial revision - rejected

Comments to author:
1. 2. 3. 4. 5. Use only one shortcut for cytochrome oxidase gene, COX1 or COI. Remove names of geographical regions from key words but add genetic distance word. Do not apply space before units, except for %. Rewrite scientific names where indicated. Please rethink using only one sample of A. marmorata in analysis. Applied at least 5 specimens and then try to indicate haplotypes again.

6. Two missing references in the text (Matoso i inni, 2004, and Felsenstein J., 1985). 7. List of references without Hebert et al., 2003. Cited in the line 188. 8. Please rethink discussion and explain 8 haplotypypes for Rasbora tawarensis species (remark what has caused such high genetic diversity?) - line 146 9. Please improve discussion. Missing comments according to C. carpio and X. helleri that were used in the analysis. In this paragraph add papers published by K. Kohlmann listed below:
Memis, D. and Kohlmann, K. (2006): Genetic characterization of wild common carp (Cyprinus carpio L.) from Turkey. Aquaculture 258: 257-262. Kohlmann, K., Kersten, P. and Flaj!hans, M. (2005): Microsatellite-based genetic variability and differentiation of domesticated, wild and feral common carp (Cyprinus carpio L.) populations. Aquaculture 247: 253-266. Lehoczky, I., Jeney, Z., Magyary, I., Hancz, C. and Kohlmann, K. (2005): Preliminary data on genetic variability and purity of common carp (Cyprinus carpio L.) strains kept at the live gene bank at Research Institute for Fisheries, Aquaculture and Irrigation (HAKI) Szarvas, Hungary. Aquaculture 247: 45-49. Kohlmann, K., Gross, R., Murakaeva, A. and Kersten, P. (2003): Genetic variability and structure of common carp (Cyprinus carpio) populations throughout the distribution range inferred from allozyme, microsatellite and mitochondrial DNA markers. Aquatic Living Resources 16: 421-431. Murakaeva, A., Kohlmann, K., Kersten, P., Kamilov, B. and Khabibullin, D. (2003): Genetic characterization of wild and domesticated common carp (Cyprinus carpio L.) populations from Uzbekistan. Aquaculture 218: 153-166.

Confidential comments to editor