Microscope Notes 1. The first microscope was invented in 1590 by Zacharias Janssen. 2. Robert oo!

e in 1""5 was the first person to #se the word ce$$s after viewin% the bar! of an oa! tree #nder the microscope. &. 'sin% the microscope a. to carry the microscope %rasp the arm with one hand and the other hand #nder the base. b. ($ace the microscope on the tab$e with the arm toward yo#. c. T#rn the coarse ad)#stment !nob to raise the body t#be d. Revo$ve the nosepiece #nti$ the $ow power ob)ectives $ens c$ic!s into p$ace e. *d)#st the diaphra%m whi$e $oo!in% thro#%h the eyepiece a$so ad)#st the mirror #nti$ yo# see a bri%ht $i%ht f. ($ace a s$ide on the sta%e. +enter the specimen over the openin% on the sta%e #se the sta%e c$ip to ho$d the s$ide in p$ace. %. ,oo! at the side and caref#$$y t#rn the coarse ad)#stment !nob #nti$ the ob)ectives a$most to#ches the s$ide

h. ,oo!in% thro#%h the eyepiece t#rn the fine ad)#stment !nob #nti$ the specimen comes into view. -. +are of a microscope never to#ch the $enses with fin%ers 5. Types of microscopes ,i%ht or compo#nd .tereocopes two eyes .cannin% e$ectron microscope #ses e$ectron to ma%nify 2 mi$$ion times $ar%er Transmission e$ectron microscopes e$ectrons pass thro#%h very thin specimes ". Two ways to ma%nify the eyepiece or oc#$ar $ens and the ob)ective /. There are three ma%nification scannin% $ow power ob)ectives hi%h power ob)ectives 0. 1b)ective Ma%nification 1c#$ar Tota$ $ens ma%nification .cannin% -2 102 -02 ,ow 102 1023 1002 power i%h -02 102 -002 power 9. foc#s specimen a$ways start with scannin%

 once foc#sed switch to $ow power ob)ectives refoc#s switch hi%h power if yo# have a cover s$ip if specimen is to dar! or to $i%ht then ad)#st the diaphra%m the $ine in eyepiece is the pointer 10. drawin% a specimen #se penci$ draw c$ear$y and $abe$ write ma%nification 11 . ma!in% a wet mo#nt %ather a thin piece of the specimen p$ace one drop of water p$ace cover s$ip at a -5 de%ree an%$e 12. stain a s$ide repeat above proced#res e2cept add one drop of stain iodine or methy$ene b$#e wipe #p e2cess stain with a paper towe$.