145

Lampiran 7. Data Kematian Larva dan Perhitungan LC50 Uji Toksisitas

Masing-masing Ekstrak Biomassa Chlorella sp.
Tabel transformasi persen ke probit
%
0
1
2
3
4

2.67

2.95

3.12

3.25

3.36

3.45

3.52

3.59

3.66

10

3.72

3.77

3.82

3.87

3.92

3.96

4.01

4.05

4.08

4.12

20

4.16

4.19

4.23

4.26

4.29

4.33

4.36

4.39

4.42

4.45

30

4.48

4.50

4.53

4.56

4.59

4.61

4.64

4.67

4.69

4.72

40

4.75

4.77

4.80

4.82

4.85

4.87

4.90

4.92

4.95

4.97

50

5.00

5.03

5.05

5.08

5.10

5.13

5.15

5.18

5.20

5.23

60

5.25

5.28

5.31

5.33

5.36

5.39

5.41

5.44

5.47

5.50

70

5.52

5.55

5.58

5.61

5.64

5.67

5.71

5.74

5.77

5.81

80

5.84

5.88

5.92

5.95

5.99

6.04

6.08

6.13

6.18

6.23

90

6.28

6.34

6.41

6.48

6.55

6.64

6.75

6.88

7.05

7.33

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

99

7.33

7.37

7.41

7.46

7.51

7.58

7.65

7.75

7.88

8.09

Sumber: Finey (1952)

1. Ekstrak Metanol
Konsentrasi
(ppm)
0*
0**
5
10
25
50
100
150
200
500
1000

Jumlah A. salina yang mati (ekor)

I
II
III
modus
0
0
0
0
0
0
0
0
2
1
2
2
4
4
5
4
5
5
2
5
7
8
7
7
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10

Keterangan: * : Kontrol pelarut

** : Kontrol media

% Mortalitas
0
0
20
40
50
70
100
100
100
100
100

146

Konsentrasi
(ppm)
0
0
5
10
25
50
100
150
200
500
1000

% Mortalitas

Log Konsentrasi (x)

0
0
20
40
50
70
100
100
100
100
100

0
0
0,699
1
1,3979
1,699
2
2,176
2,301
2,699
3

Probit %
Mortalitas (y)
4,16
4,75
5
5,52
-

Probit % Mortalitas

Grafik Hubungan Log Konsentrasi (x) dengan Probit %

Mortalitas (y) Ekstrak Metanol Biomassa Chlorella sp.

8
7
6
5
4
3
2
1
0

y = 1,26x + 3,3468
R = 0,9605

0,5

1,5

Log Konsentrasi

Penentuan nilai LC50 ekstrak metanol:

y
5
5 - 3,3468
x
Antilog x
LC50

= 1,26x + 3,3468
= 1,26x + 3,3468
= 1,26x
= 1,3121
= 20,516
= 20,516 ppm

2,5

3,5

147

Penentuan rentang nilai LC50 (Upper Limit dan Lower Limit) (Reish dan Oshida,
1987):
Menghitung nilai S (standard error)
LC50 = 20,516
y

LC16

84

S=

50

= 1,26x + 3,3468

y = 4,01
4,01 = 1,26x + 3,3468
4,01-3,3468 = 1,26x
x = 0,526
antilog x = 3,357
LC16 = 3,357
+

50
16

125 ,314 20,516

+
20,516
3,357

LC84

y = 5,99
5,99 = 1,26x + 3,3468
5,99-3,3468 = 1,26x
x = 2,098
antilog x = 125,314
LC84 = 125,314

= 6,110

Log S = 0,786
Menghitung nilai N (total jumlah hewan uji yang mati diantara nilai LC16
smpai LC84)
N= 6+12+15+21+30 = 84
Menghitung nilai f
Log f =

2,77

x log S =

2,77
84

x 0,786 = 0,273
f

= 1,728

Menghitung Upper dan Lower Limit

Upper Limit =LC50 x f = 20,516 x 1,728 = 35,452
Lower Limit =

LC 50

20,516
1,728

= 11,873

Rentang nilai LC50 ekstrak metanol= 11,873 35,452 ppm

148

2. Ekstrak Etil Asetat

Jumlah A. salina yang mati (ekor)
Konsentrasi
(ppm)
I
II
III
modus
0*
0
0
0
0
0**
0
1
0
0
5
0
0
3
0
10
0
0
0
0
25
0
1
0
0
50
0
0
2
0
100
2
0
0
0
150
3
3
0
3
200
10
8
8
8
500
10
10
7
10
1000
10
10
10
10

% Mortalitas
0
0
0
0
0
0
0
30
80
100
100

Keterangan: * : Kontrol pelarut

** : Kontrol media

Konsentrasi
(ppm)
0
0
5
10
25
50
100
150
200
500
1000

% Mortalitas

Log Konsentrasi (x)

0
0
0
0
0
0
0
30
80
100
100

0
0
0,699
1
1,3979
1,699
2
2,176
2,301
2,699
3

Probit %
Mortalitas (y)
4,48
5,84
-

149

Grafik Hubungan Log Konsentrasi (x) dengan Probit %

Mortalitas (y) Ekstrak Etil Asetat Biomassa Chlorella sp.

Probit % Mortalitas

15
y = 10,88x - 19,195
R = 1

10
5
0
-5

0,5

1,5

2,5

3,5

-10
-15

Log Konsentrasi

Penentuan LC50 ekstrak etil asetat:

y
= 10,88x - 19,195
5
= 10,88x - 19,195
5 + 19,195 = 10,88x
x
= 2,2238
Antilog x = 167,417
LC50
=167, 417 ppm
Penentuan rentang nilai LC50 (Upper Limit dan Lower Limit) (Reish dan Oshida,
1987):
Menghitung nilai S (standard error)
LC50 = 167,417
y

LC84
y = 4,01
4,01 = 10,88x - 19,195
4,01+19,195 =10,88x
x = 2,133
antilog x = 135,831
LC16 = 135,831

LC16

84

S=

50

= 10,88x - 19,195

50
16

Log S = 0,091

206 ,538 167 ,417

+
167 ,417 135 ,831

= 1,233

y = 5,99
5,99 = 10,88x - 19,195
5,99+19,195= 10,88x
x = 2,315
antilog x = 206,538
LC84 = 206,538

150

Menghitung nilai N (total jumlah hewan uji yang mati diantara nilai LC16
smpai LC84)
N= 9+24+30 = 63
Menghitung nilai f
Log f =

2,77

x log S =

2,77
63

x 0,091= 0,032
f

= 1,076

Menghitung Upper dan Lower Limit

Upper Limit =LC50 x f = 167,417 x 1,076= 180,141
Lower Limit =

LC 50

167,417
1,076

= 155,592

Rentang nilai LC50 = 155,592 180,141 ppm