ISSN 1678-0493

Dilogos & Cincia

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#iologia "$ Sist$%as "$ &$ctinas$s "o '(ngo Moniliophthora perniciosa

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Centro de Biotecnologia e Genetica - DCB, Universidade Estadual de Santa Cruz (UESC), Ilh us, B!, Brazil Centre de Coop ration Internationale en #echerche !grono$i%ue pour le D veloppe$ent (CI#!D), UM# !G!&, Montpellier, 'rance doi( )*+,--,.dc+/*)0+**1

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?)SL*< +s 1$ctinas$s 71$ctina-%$til$st$ as$s N&*)sO- 1oligalact( onas$s N&CsO $ 1 oto1$ctinas$s8 s2o $nPi%as 5($ "$g a"a% s(bstQncias 13cticas- 1 inci1ais constit(int$s "a la%$la %3"ia "os ,$g$tais. 'ito1atMg$nos 1 o"(P$% tais $nPi%as- 5($ s2o "$ f(n"a%$ntal i%1o tQncia 1a a a s(a ,i (lncia. + ,asso( a "$ b (4a"o$nAa ca(sa"a 1$lo basi"io%ic$to 0$%ibiot Mfico- Moniliophthora perniciosa- t$% co%1 o%$ti"o a caca(ic(lt( a no s(l "a #a0ia. Da"os "o s$5($ncia%$nto "o g$no%a "$ss$ 1atMg$no $,$la a% a 1 $s$nAa "$ g$n$s 0o%Mlogos a 1$ctinas$s. < obR$ti,o "$ss$ t abal0o foi analisa (%a $"$ "$ int$ aA2o 1 ot$Gna-1 ot$Gna obti"a 1o biologia "$ sist$%as- g$ a"a a 1a ti "$ (%a 1oligalact( onas$ "$ M+ perniciosa 7*1&C=8. &a a tal- foi f$ita (% b(sca "o o tMlogo $% 8eurospora crassa (tiliPan"o o #last $,$ so 7reciprocal Blast8. + $"$ foi %onta"a no 0tt1B!!st ing-"b.o g! co% o o tMlogo NCL0=369- $ a1 $s$nto( 683 nMs. &a a o $st("o "a $"$- foi (tiliPa"o o 1 og a%a CDtosca1$- no 5(al fo a% $aliPa"as anlis$s i8 "$ c$nt ali"a"$- 5($ $,$lo( 30 ga galos "$nt $ os 5(ais a *1&C= s$ $ncont o(- 149 0(bs $ 14= 0(bs-ga galos> $ ii8 "$ %o"(la i"a"$- on"$ foi 1ossG,$l i"$ntifica s$is clusters> $ iii8 "$ ontologia gnica. + NCL10049 3 (%a 1$ctin$st$ as$ 5($ a1a $c$( na $"$ int$ agin"o co% a NCL0=369-*1&C=- $la $laciona-s$ co% o(t as 1 ot$Gnas co%o a NCL09063 5($ 3 (%a glicosil 0i" olas$ "a 1a $"$ c$l(la - al3% "$ int$ agi co% a NCL063=6.1- 5($ 3 (%a 1$ctato lias$-1. +ssi%- foi 1ossG,$l inf$ i 5($ 1$ctinas$s $ alg(%as 0i" olas$s $ncont a"as na $"$- int$ ag$% "i $ta o( in"i $ta%$nt$ co% a NCL0=369-*1&C=$(nin"o assi% (% conR(nto "$ ati,i"a"$s $nPi%ticas 5($ 1o"$% s$ "$t$ %inant$s 1a a a in,as2o "o 0os1$"$i o. +#SI?+CI TITLE: Systems biology of Moniliophthora perniciosa e!tin"ses &$ctinas$s 71$ctin %$t0Dl$st$ as$s N&*)sO- 1olDgalact( onas$s N&CsO an" 1 oto1$ctinas$s8 a $ $nPD%$s in,ol,$" in t0$ "$g a"ation of t0$ 1$ctic %ol$c(l$- w0ic0 is t0$ %ain co%1on$nt of t0$ 1lant %i""l$ la%$lla. &0Dto1at0og$ns 1 o"(c$ s(c0 $nPD%$s- w0ic0 a $ ,$ D i%1o tant fo t0$i ,i (l$nc$. I0$ witc0$sU b oo% "is$as$- ca(s$" bD t0$ 0$%ibiot o10ic basi"io%Dc$t$ Moniliophthora perniciosa- 0as co%%itt$" t0$ cacao 1 o"(ction in So(t0 #a0ia- # aPil. Moniliophthora perniciosa g$no%$ s$5($ncing $,$al$" t0$ 1 $s$nc$ of 0o%ologo(s of 1$ctinas$ g$n$s. I0$ obR$cti,$ of t0is wo V was to obtain a 10Dsical 1 ot$in-1 ot$in int$ action n$two V bD sDst$%s biologD- g$n$ at$" f o% a M+ perniciosa 1olDgalact( onas$ 7*1&C=8. I0$ s$a c0 fo 8eurospora crassa *1&C= o t0olog was 1$ fo %$" (sing t0$ $ci1 ocal #last. I0$ n$two V was obtain$" in 0tt1B!!st ing-"b.o g! wit0 t0$ NCL0=369 o t0olog an" 1 $s$nt$" 683 no"$s. 'o t0$ n$two V st("D- w$ (s$" t0$ CDtosca1$ softwa $ in w0ic0 t0$ following analDs$s w$ $ %a"$B i8 c$nt alitD w0ic0 $,$al$" 30 bottl$n$cVs incl("ing t0$ *1&C=- 149 0(bs an" 14= 0(bs-bottl$n$cV> ii8 %o"(la itD w0ic0 allow$" t0$ i"$ntification of 6 cl(st$ s> an" iii8 g$n$ ontologD. I0$ NCL10049 is a 1$ctin$st$ as$ w0ic0 int$ acts in t0$ n$two V wit0 NCL0=369-*1&C=> it was also $lat$" to ot0$ 1 ot$ins s(c0 as t0$ NCL09063 glDcosDl 0D" olas$ o wit0 t0$ NCL063=6.1 1$ctat$ lDas$-1. I0(s- it was 1ossibl$ to s(gg$st t0at 1$ctinas$s an" 0D" olas$s w$ $ fo(n" in t0$ n$two V an" int$ act$" "i $ctlD o in"i $ctlD wit0 NCL0=369-*1&C= fo %ing a s$t of $nPD%atic acti,itD t0at %aD b$ c (cial fo 0ost in,asion. W$Dwo "sB witc0$sU b oo% "is$as$- 1$ctinas$s- 1lant-1at0og$n int$ action

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1. Introduo +s 1$ctinas s2o %ol3c(las co%1l$4as "$ ci"o 1oligalact( /nico 5($ cont3% $gi6$s s$ooth 7n2o a%ifica"as8 $ hair2 7 a%ifica"as8- $ 5($ constit($% $% %3"ia 39: "a 1a $"$ 1 i% ia "os ,$g$tais s(1$ io $s 7C+''+;; & *<.N)N- =009> ?ID;)@ $t al- =0018. + %ol3c(la "$ 1$ctina 3 co%1osta "$ ,a ias $gi6$s 7"$ aco "o co% o ti1o "$ a%ificaA2o $ co%1osiA2o8B 0o%ogalact( onano 7.C8a%nogalact( onano I 7?CI8- a%nogalact( onano II 7?CII8 $ 4Dlogalact( onano 7EC+8 7FISS)?- 19968. < .C 3 (%
Di3logos 4 Ci5ncia, no 00, $ar6o de /*)0

1olG%$ o lin$a 5($ consist$ $% (%a ca"$ia 1 inci1al fo %a"a 1o ci"os D-galact( /nicos- os 5(ais 1o"$% $ncont a -s$ ac$tila"os $!o( %$til-$st$ ifica"os> ii8 o ?CI consist$ $% (%a ca"$ia 5($ alt$ na $sG"(os "$ ci"o galact( /nico co% $sG"(os "$ a%nos$- nos 5(ais 1o"$% s$ $ncont a"as liga"as co,al$nt$%$nt$ ca"$ias lat$ ais "$ $sG"(os "$ aAHca $s n$(t os co%o galactos$ $ a abinos$> iii8 o ?CII 1oss(i ca"$ia lin$a "$ 0o%ogalact( onano co% ca"$ias lat$ ais co%1l$4as liga"as aos $sG"(os "$ galact( onato> $ i,8 o EC+ 5($ consist$ $% (%a ca"$ia co%1osta "$ ci"o galact( /nico $ $sG"(os "$ 4ilos$ 7.+?.<;I $t al- =0108. S$g(n"o a
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Soci$"a"$ +%$ icana "$ X(G%ica 7 !$erican Che$ical Societ28 3 1ossG,$l classifica os ca boi" atos 13cticos $%B i8 1 oto1$ctina> ii8 1$ctina> iii8 ci"o 13ctico> $ i,8 ci"o 1$ctGnico. )ssa classificaA2o $st bas$a"a $% s(a sol(bili"a"$ $% g(a $ g a( "$ %$tilaA2o- on"$ as 1$ctinas s2o as %ais solH,$is $ 1oss($% s(a ca"$ia 1oligalact( /nica co% "if$ $nt$s g a(s "$ %$to4ilaA2o. )%bo a co%1l$4os- os ca boi" atos 13cticos s2o facil%$nt$ "$g a"a"os 1o $nPi%as 13cticas 71$ctinas$s8 5($ 1o"$% s$ classifica"as $% 1 oto1$ctinas$s- 1$ctin$st$ as$ $ $nPi%as "$s1oli%$ iPant$ 7Y+@+NI $t al- =0098 "$ aco "o co% o s(bst ato $ a $gi2o "o $s5($l$to 1oligalact( /nico no 5(al at(a 7+;W<?I+ 19988. +s 1 oto1$ctinas$s ag$% sob $ a 1 oto1$ctina to nan"o-a solH,$l. +s 1$ctina %$til$st$ as$s 7&*)s8 at(a% con,$ t$n"o 1$ctina alta%$nt$ $st$ ifica"a a (%a fo %a "$ bai4a %$to4ilaA2o. Nas $nPi%as "$s1oli%$ iPant$s- $ncont a%-s$ as 1oligalact( onas$s 7&Cs8 5($ o%1$% as ligaA6$s Z-71-48 $nt $ os $sG"(os "$ ci"o galact( /nico n2o $st$ ifica"os. +s &Cs s$ "$staca% "$nt $ as 1$ctinas$s 1o s$ $% "$ g an"$ i%1o tQncia 1a a os 1atMg$nos na coloniPaA2o "o 0os1$"$i o 7;+NC $ D[?N)N#L?C=0008. )las s2o classifica"as $% i8 $n"o-&Cs 5($ 0i" olisa% o $s5($l$to 1oligalact( /nico al$ato ia%$nt$> $ ii8 $4o-&Cs 5($ o%1$% as ligaA6$s glicosG"icas Z-71-48 t$ %inais. +lg(%as "$stas $nPi%as t$% aA6$s s$5($nciais co%o o caso "as &*)s 5($ g$ a% 1$ctina "$ bai4a %$to4ilaA2o l$,an"o a (%a at(aA2o sin3 gica co% as &Cs- (%a ,$P 5($ $stas at(a% sob $ a 1$ctina "$ bai4a %$to4ilaA2o. D$ (%a fo %a g$ al- as 1$ctinas$s s2o 1 o"(Pi"as 1o (%a ga%a "$ %ic o ganis%os co%o f(ngos fila%$ntosos- l$,$"( as $ bact3 ias 7Y+@+NI $t al- =0098. Co% $ss$ conR(nto "$ ati,i"a"$s $nPi%ticas- os 1atMg$nos "$ 1lantas 1assa% a t$ g an"$ $ficincia na in,as2o "$ s$( 0os1$"$i o. No caso "$ Moniliophthora perniciosabasi"io%ic$to 0$%ibiot Mfico ca(sa"o "a "o$nAa ,asso( a-"$-b (4a $% caca(- fo a% i"$ntifica"os t s g$n$s "$ &Cs. + 1 ot$ina *1&C= 3 o c$nt o "o 1 $s$nt$ $st("o. D$,i"o a s(a co%1l$4i"a"$- a co%1 $$ns2o "$ (% sist$%a biolMgico $4ig$ $c( sos s$g( os $ a"$5(a"os 1a a o s$( $st("o- $ a biologia "$ sist$%as co%1(tacional s$ can"i"ata co%o (%a "as %$l0o $s f$ a%$ntas 1a a $ssa finali"a"$ 7WII+N<- =00=8. +s $"$s "$ int$ aA2o 7 net9or:s8 fGsica 1 ot$Gna-1 ot$Gna s2o f$ a%$ntas "$ g an"$ i%1o tQncia no $st("o "os 1 oc$ssos biolMgicos $% (% "$t$ %ina"o o ganis%o- a fi% "$ s$ c ia %o"$los $% "if$ $nt$s $scalas "$ o ganiPaA2o biolMgica 5($ $41li5($ o s$( f(nciona%$nto 7#);I?+< C+CN)@ $ W?<C+N- =0108. #as$an"o-s$ n$ssas int$ aA6$s- 3 1ossG,$l $st("a co%1l$4os 1 ot$icos $ associ-los a s(as $s1$cti,as f(nA6$s- fato 5($ coloca a biologia "$ sist$%as $% "$sta5($- 1o "a (% g an"$ s(1o t$ na anlis$ "$ "a"os g$ a"os 1o anlis$s "$ la ga $scala- co%o 1 ot$/%ica o( t ansc i1t/%ica- "$nt $ o(t as 7ID)W)? & W?<C+N- =01=8. &a a a const (A2o $ anlis$ "$ $"$s biolMgicas- a bioinfo %tica a1 $s$nta (% f(n"a%$ntal 1a1$l- "$s"$ o s(1o t$ na b(sca "$ o tMlogos co% o (so "$ algo Gti%os co%o o #;+SI 7Basic ;ocal !lign$ent Search 7ool8- at3 a "is1onibili"a"$ "$ bancos "$ "a"os on-line co%o o SI?INC\ 7Search 7ool <or the #etrieval o< Interacting Genes > S]W;+?C]@W $t al.- =0118- o 5(al 1 $"iP $ %o"$la $"$s t i"i%$nsionais- bas$an"o-s$ $% info %aA6$s "$ s(as int$ aA6$s fGsica $ f(ncionais conti"as $% o(t os bancos "$ "a"os- co%o 1o $4$%1lo- o Lni& ot\ 7*+C?+N) & C<NS<?IIL*- =0118- S*+?I\ 7;)ILNIC $t al- =0098C$n$Ca "s\ 7S+'?+N $t al.- =0108 $ S^ISS-*<D); 7WI)')? $t al- =0098. +s anlis$s co%1(tacionais "a biologia "$ sist$%as conta% ta%b3% co% f$ a%$ntas o<<-line co%o o CDtosca1$\ 7S.+NN<N- =0038. < Molecular Co$ple= Detection 7*C<D)\8 3 (% softwa $ 5($ 3 $ncont a"o co%o plugin 1a a o CDtosca1$- $ 5($ g$ a s(b-g afos 7cluster8 a

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MpPG2 XP_959790.2 M PG2 XP_959790.2 M PG2 XP_959790.2 M PG2 XP_959790.2 38 104 9! 151 157 20! 215 2#0 GNGPEYWDGLGGNGGVTKPAPMMKYVVESSELSQNIDRIKISGVYQDVVVVNSPARTYSV 97 G+G +YWDGLG G + KP + +E S V + V ++N+P + S+ GSGEKYWDGLGQKGPIKKPKFFQVHNLEDS-------------VIEGVTILNAPVQVMSI 150 SNPGPLLITSLTIDNSLGDEPNDQSNGEPAGHNTDGFD"-STHDLVISNSVIHNQDD"LA + L +TS T+DN GD D G+ G NTD FD S+ ++VI + ++NQDD"+A NG"KNLTVTSFTLDNKAGD--GDWKAGK-GG$NTDAFDIGSSSNIVIDGAKVYNQDD"VA INKGSNITFSGNT"TGGHGISVGSI--SSDAVVTDIHITNNMIIDKLVQLAKPYDQAL$I IN G++ITF "+GGHG+S+GS+ SD V ++ N++I + + $I INSGTDITF$NGL"SGGHGLSIGSVGG$SDNTVKNVLFENSVIANS--------ENGA$I KTKADATSASVSGVTYSGNTATG"K$FGVIIDQ KT T+ VS +TY ++G+++DQ KTNY-GTTGLVSNITY$NVKLQNITKYGIMVDQ 247 291 15# 207 214 259

XP_959790.2 $&'M (&) XP_959790.2 $&'M (&) XP_959790.2 $&'M (&) XP_959790.2 $&'M (&)

79 12 1%4 71 1!1 1%1 241 1!!

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1%% 70 1!0 1%0 240 1!7 291 247

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$&'M (&) M PG2 $&'M (&) M PG2 $&'M (&) M PG2 $&'M (&) M PG2 $&'M (&) MpPG2 1 1 #1 #1 121 121 1!1 1!1 241 241 MNGDITFGVANWEGPLFTLKIP$LTAITVNGNG$KFDGNGPEYWDGLGGNGGVTKPAPMM MNGDITFGVANWEGPLFTLKIP$LTAITVNGNG$KFDGNGPEYWDGLGGNGGVTKPAPMM MNGDITFGVANWEGPLFTLKIP$LTAITVNGNG$KFDGNGPEYWDGLGGNGGVTKPAPMM KYVVESSELSQNID$IKISGVYQDVVVVNSPA$TYSVSNPGPLLITSLTIDNSLGDEPND KYVVESSELSQNID$IKISGVYQDVVVVNSPA$TYSVSNPGPLLITSLTIDNSLGDEPND KYVVESSELSQNID$IKISGVYQDVVVVNSPA$TYSVSNPGPLLITSLTIDNSLGDEPND QSNGEPAGHNTDGFD"STHDLVISNSVIHNQDD"LAINKGSNITFSGNT"TGGHGISVGS QSNGEPAGHNTDGFD"STHDLVISNSVIHNQDD"LAINKGSNITFSGNT"TGGHGISVGS QSNGEPAGHNTDGFD"STHDLVISNSVIHNQDD"LAINKGSNITFSGNT"TGGHGISVGS ISSDAVVTDIHITNNMIIDKLVQLAKPYDQAL$IKTKADATSASVSGVTYSGNTATG"K$ ISSDAVVTDIHITNNMIIDKLVQLAKPYDQAL$IKTKADATSASVSGVTYSGNTATG"K$ ISSDAVVTDIHITNNMIIDKLVQLAKPYDQAL$IKTKADATSASVSGVTYSGNTATG"K$ FGVIIDQVNPEAE FGVIIDQVNPEAE FGVIIDQVNPEAE 25% 253 #0 #0 120 120 1!0 1!0 240 240

'igura )( <bt$nA2o "o o tMlogo "$ *1&C= $% 8eurospora crassa. +. +lin0a%$nto "$ *1&C= co% o Best hit 71 $c( so "$ 1oligalact( onas$ E&_999790.=8 "o #last1 "$ *1&C= cont a o banco "$ s$5(ncias "$ 8+ crassa. #. +lin0a%$nto "$ E&_999790.= co% o Best hit 7?$c*1s$58 "o #last1 "$ E&_999790.= cont a o banco "$ s$5(ncias "$ M+ perniciosa 7reciprocal "last8. C. +lin0a%$nto "as "(as s$5(ncias 7*1&C= $ $s(lta"o "o reciprocal "last8 "$ M+ perniciosa+

1a ti "$ (%a $"$ biolMgica bas$an"o-s$ $% 1 oc$ssos biolMgicos- o 5($ 1$ %it$ (% %$l0o $st("o "os co%1l$4os 1 ot$icos 7#+D)?- =0038. < $st("o "os 1 oc$ssos c$l(la $sbiolMgicos o( %ol$c(la $s 1o"$ s$ f$ito co% o a(4Glio "a Biological 8et9or:s Gene >ntolog2 tool 7#iNC<\87*+)?) $t al- =0098. <(t o i%1o tant$ algo it%o 3 o C$ntiSca&$\- $l$ "$t$ %ina a c$nt ali"a"$ 7o( $l$,Qncia no 5($ "iP $s1$ito a int$ aA2o "$ ca"a nM na $"$8. D$nt $ as c$nt ali"a"$s t$%os- 1o $4$%1loB i8 o Degree- 5($ co $s1on"$ ao g a( "$ con$cti,i"a"$ "$ (% nM a nMs a"Rac$nt$s> o s$( ,alo 3 "$ g an"$ i%1o tQncia 1a a a "$t$ %inaA2o "o nH%$ o "$ int$ aA6$s 5($ faP (% "$t$ %ina"o nM $% (%a $"$> $ ii8 o Bet9eenness 7SC+?D<NI $t al- =0098 5($- s$g(n"o @L $t al 7=0078- 3 (%a "as 1 o1 i$"a"$s %ais i%1o tant$s na to1ologia "a $"$ $ 3 (tiliPa"a 1a a "isc i%ina nMs ga galos 7"ottlenec:s8. $. M"teri"l e M%todos + b(sca "a s$5(ncia o tMloga a 1 ot$Gna *1&C= $% 8eurospora crassa- foi $aliPa"a no NC#I 78ational Center <or Biotechnolog2 In<or$ation> 0tt1B!!www.ncbi.nl%.ni0.go,8(san"o o algo it%o #last1 71 ot$Gna cont a 1 ot$Gna8. < $s(lta"o "$ %aio e-value foi (sa"o co%o 1ossG,$l can"i"ato ` 1 ot$Gna o tMloga "a *1&C=. )% s$g(i"a- foi f$ito (% #last1 $,$ so 7reciprocal "last8- co% a s$5(ncia "$ 8+ crassa- cont a M+ perniciosa. + $ci1 oci"a"$ foi ,$ ifica"a 5(an"o o Best hit 7%aio e-value8 alin0a,a 100: a s$5(ncia o iginal "$ *1&C=. + s$5(ncia "$ 8+ crassa o tMloga "a *1&C= 7NC#IB
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SI;F+ $t al.- Di3logos 4 Ci5ncia 00- =013- =7-30 in,a"$ o caca($i o at a,3s "$ s$(s t$ci"os %$ ist$%ticos 7&L?D@ $ SC.*IDI- 19968- 5($ s2o icos $% s(bstQncias 13cticas- o 5($ nos l$,a a inf$ i 5($ o co%1l$4o $41osto na 'ig( a =- $%bo a s$Ra (% %o"$lo 1a a 8+ crassa 1o"$ %(ito b$% $sta 1 $s$nt$ $% M+ perniciosa- ,isto 5($ a $"$ foi %onta"a co% (%a 1 ot$Gna o tMloga a *1&C=. I abal0os "$ co%1a aA2o "as s$5(ncias "$ssa $"$ co% o g$no%a "o M+ perniciosa 1o"$ ,i a $,$la %$l0o $s info %aA6$s sob $ a 0o%ologia "o co%1l$4o 1 ot$ico co%o (% to"o $ $sta confi %an"o a $4istncia "$ 1 oc$ssos bastant$ si%ila $s. ). Agr"de!imentos < t abal0o "$ .+SC foi fo%$nta"o 1$la Coo "$naA2o "$ +1$ f$iAoa%$nto "$ &$ssoal "$ NG,$l S(1$ io 7C+&)S8. < t abal0o "$ )*+S foi fo%$nta"o 1$lo Centre de Coop ration Internationale en #echerche !grono$i%ue pour le D veloppe$ent 7CI?+D8 $ $% s$g(i"a 1$lo Cons$l0o Nacional "$ D$s$n,ol,i%$nto Ci$ntGfico $ I$cnolMgico 7CN&58. )sta 1$s5(isa foi financia"a 1$lo #anco "o No "$st$ 7#N#8 $ 1$la Lni,$ si"a"$ )sta"(al "$ Santa C (P 7L)SC8. *. 'efer+n!i"s +;W<?I+- I.> C+?#ISL- C.> ;;+*+- *. Y.> S)??+- Y. ;. In"(st ial a11lications of 1$ctic $nPD%$sB a $,i$w. &rocess Bioche$istr2- ,. 33- n. 1- 1. =1-=8. 1998. #+D)?- C. D. an" .<CL)-C.^. +n a(to%at$" %$t0o" fo fin"ing %ol$c(la co%1l$4$s in la g$ 1 ot$in int$ action n$two Vs. BMC Bioin<or$atics- ,. 4- n. ==003. #);I?+<- &.> C+CN)@- C.> W?<C+N- N. Y. X(antitati,$ g$n$tic int$ actions $,$al biological %o"(la itD. Cell, ,. 141. n. 9- 1. 739-749. =010. C+''+;;- W. ..> *<.N)N- D. I0$ st (ct( $- f(nction- an" biosDnt0$sis of 1lant c$ll wall 1$ctic 1olDsacc0a i"$s. Car"oh2drate research- ,. 344- n. 14- 1. 1879-1900. =009. .+?.<;I- Y.> SLII+NCW+WL;- +.> FI#) SC.);;)?.. #iosDnt0$sis of 1$ctin. &lant ph2siolog2- ,. 193- n. =- 1. 384-399. =010. ID)W)?- I.> W?<C+N- N. Y. Diff$ $ntial n$two V biologD. Molecular s2ste$s "iolog2- ,. 8- n. 969- 1. 1744-4=9=. =01=. Y+@+NI- ?. S.> S+E)N+- S.> CL&I+- ?. *ic obial 1$ctinolDtic $nPD%$sB + $,i$w. &rocess Bioche$istr2,. 40- n. 9- 1. =931b=944. =009. WI)')?- '.> +?N<;D- W.> WcN];I- *.> #<?D<;I- ;.> SC.^)D)- I. I0$ S^ISS-*<D); ?$1osito D an" associat$" $so( c$s. 8ucleic acids research- ,. 37- n. 1- 1. 387b39=. =008. WII+N<- .. Co%1(tational sDst$%s biologD. 8ature- ,. 4=0n. 691=- 1. =06b=10. =00=. ;+NC- C.> D[?N)N#L?C- .. &$ s1$cti,$s in t0$ biological f(nction an" t0$ t$c0nological 19 a11lication of 1olDgalact( onas$s. !pplied Micro"iolog2 and Biotechnolog2- ,. 93- n. 4- 1. 366- 379. =000. ;)ILNIC- I.> D<)?WS- I.> #<?W- &. S*+?I 6B $c$nt (1"at$s an" n$w "$,$lo1%$nts. 8ucleic acids research,. 37- n. 1- 1. ==9b=3=. =009. *+)?)- S.> .)@*+NS- W.> WLI&)?- *. #iNC<B a CDtosca1$ 1l(gin to ass$ss o,$ $1 $s$ntation of g$n$ ontologD cat$go i$s in biological n$two Vs. Bioin<or$atics- ,. =1- n. 16- 1. 3448-3449. =009. *+C?+N)*.> C<NS<?IIL*L. Lni& ot Wnowl$"g$bas$B a 0(b of int$g at$" 1 ot$in "ata. Data"ase( the Aournal o< "iological data"ases and curation- ,. =011- ba 009- 1. 1-13. =011. &L?D@- ;.> SC.*IDI- ?. Stat(s of cacao witc0$sd b oo%B #iologD- )1i"$%iologD- an" =9 *anag$%$nt. !nnual
#ede de ensino '7C

'igura /( ?$s(lta"o "as anlis$s f$itas 1a a a $"$ %onta"a co% a *1&C= 7NCL0=3698> +- $"$ obti"a no SI?INC %ol"a"a no CDtosca1$> #- cl(st$ 1> C- cl(st$ => D- cl(st$ 3> )- cl(st$ 4> 'cl(st$ 9> C- cl(st$ 6.

E&_999790.=8 foi (sa"a 1a a %o"$la (%a $"$ "$ int$ aA2o 1 ot$Gna-1 ot$Gna no SI?INC data"ase 70tt1B!!st ing-"b.o g8on"$- a 1 incG1io- n2o foi s$l$ciona"o n$n0(% o ganis%o co%o $f$ ncia. 'oi "$t$cta"a (%a 1 ot$Gna 0i1ot3tica "$ N. c assa co% 379 $sG"(os "$ a%inoci"os "$no%ina"a NCL0=369. <s 1a Q%$t os (tiliPa"os 1a a a a"iA2o "$ nMs a $"$ fo a%B n2o %ais 5($ 90 int$ aA6$s- bai4a confianAa 70-1908 $ foi $5($ i"o a a"iA2o "$ 800 nMs. 'oi 1ossG,$l obt$ (%a $"$ sat( a"a co% 683 nMs. &a a a %in$ aA2o "os "a"os- a %at iP "is1onibiliPa"a 1$lo SI?INC no fo %ato t$4to foi (tiliPa"a no CDtosca1$- on"$ a $"$ "$ int$ aA2o 1 ot$ica foi $%o"$la"a $ analisa"a co% o *C<D)\- no 5(al fo a% s$l$ciona"os os %M"(los 5($ a1 $s$nta a% (% sco $ a 1a ti "$ =-9. &a a o $st("o "os 1 oc$ssos biolMgicos nos 5(ais as 1 ot$Gnas "a $"$ $ os co%1l$4os 1 ot$icos obti"os 1$lo *C<D) $sta,a% $n,ol,i"os- foi (tiliPa"o o #iNC<- co% t$st$ $statGstico 0i1$ g$o%3t ico $ t$st$s %Hlti1los "$ co $A2o 7#$nRa%ini & .oc0b$ gUs 'D? co% nG,$l "$ significQncia "$ 0-098. < plugin C$ntiSca&$ foi a1lica"o s$l$cionan"o a $"$ 1 inci1al $ a "i $cionan"o 1a a a analis$ "as c$nt ali"a"$s Degree $ Bet9eenness- a fi% "$ "$t$cta os nMs 0(bs- ga galos $ 0(bs-ga galos. &a a %$l0o co%1 $$ns2o "$ $"$ %o"$la"afoi $aliPa"a (%a b(sca "a f(nA2o "$ ca"a 1 ot$Gna no banco "$ "a"os "$ 8+ crassa 70tt1B!!www.b oa"instit(t$.o g !annotation!g$no%$!n$( os1o a!*(lti.o%$.0t%l8. &. 'esult"dos e (is!usso Blastp reverso < #last1 "a *1&C= cont a 8eurospora crassa t$,$ co%o b$st 0it (% 1 $c( so "$ 1oligalact( onas$ 7E&_999790.=8. < alin0a%$nto t$,$ (%a cob$ t( a "$ 83:- e-value "$ 1$-=9 $ i"$nti"a"$ %4i%a "$ 39: 7'ig( a 1+8. < 1 $c( so "$ 1oligalact( onas$ E&_999790.= foi- $% s$g(i"a- co%1a a"o 7reciprocal Blast8 co% o banco "$ "a"os "$ M+ perniciosa- o 5($ $s(lto( no alin0a%$nto a1 $s$nta"o na 'ig( a 1#- $st$ t$n"o (%a cob$ t( a "$ 98:- e-value "$ 7$-31 $ i"$nti"a"$ %4i%a "$ 34:. < alin0a%$nto "as "(as s$5($ncias "$ M+ perniciosa (ant$s $ "$1ois "o reciprocal Blast) a1 $s$nto( i"$nti"a"$ $ cob$ t( a "$ 100:- al3% "$ e-value "$ 0-0 7'ig( a =C8- assi% 1o"$%os consi"$ a 5($ o o tMlogo "$ *1&C= $% 8+ crassa 3 ,$ "a"$i o. #ede de intera6?o prote@na-prote@na 'oi obti"a (%a $"$ g$ al "$ int$ aA6$s fGsicas $ f(ncionais cont$n"o 683 nMs $ =0997 con$cto $s- al3% "os 6 clusters g$ a"os 1$lo *C<D)\ 7'ig( a =8. 'o a% "$t$cta"os
'igura 0B Co%1l$4o 1 ot$ico $n,ol,i"o na "$s a%ificaA2o $ "$g a"aA2o "a 1$ctina.

30 ga galos> "$nt $ $l$s- s$t$ int$ ag$% co% a o tMloga "a *1&C=- NCL0=369. . (%a 1$ctin$st$ as$ 7NCL1004985($ at(a $"(Pin"o o g a( "$ %$to4ilaA2o "$ 1$ctinasfa,o $c$n"o a aA2o "a 1oligact( onas$. Na 'ig( a 3 1o"$%os obs$ ,a (% co%1l$4o 1 ot$ico 5($ $n,ol,$ a NCL0=369 $ NCL10049. a ta%b3% 1ossG,$l nota (%a ,a i$"a"$ "$ $nPi%as $laciona"as ` "$s a%ificaA2o $ "$g a"aA2o "a 1$ctina- fato 5($ to na o s(bst ato 1oligalact( /nico %ais ac$ssG,$l `s 1$ctinas$s. *o"$los c ia"os 1a a 8+ crassa $,$la a% 5($ %$tabMlitos g$ a"os 1$la "$g a"aA2o "a 1a $"$ c$l(la - co%o 4ilos$glicos$- c$lobios$- 1o $4$%1lo 1o"$% $sta %o"(lan"o a $41 $ss2o gnica "$ $nPi%as co%o c$l(las$ $ 0$%ic$l(las$s 7C.+<CL+NC II+N $t al.- =0098. < f(ngo M+ perniciosa
Di3logos 4 Ci5ncia, no 00, $ar6o de /*)0

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SI;F+ $t al.- Di3logos 4 Ci5ncia 00- =013- =7-30

#evie9 o< &h2topatholog2- ,. 34- 1. 973-994. 1996. ?ID;)@- #. ;.> <UN)I;;- *. +.> *<.N)N- D. &$ctinsB st (ct( $- biosDnt0$sis- an" oligogalact( oni"$- $lat$" signaling. &h2toche$istr2- ,. 97- n. 6- 1. 9=9b967. =001. S+'?+N- *.> D+;+.- I.> +;)E+ND)?- Y.> ?<S)N- N.> IN@ SI)IN- I.> S.*<IS.- *.> N+IIF- N.> )I +;. C$n$Ca "s F$ sion 3B t0$ 0(%an g$n$ int$g ato . Data"ase( the Aournal o< "iological data"ases and curation- ,. =010- ba50=0- 1. 1-16. =010. SC+?D<NI- C.> &)II)?;INI- *.> ;+LD+NN+- C. +nalDPing biological n$two V 1a a%$t$ s wit0 C$ntiSca&$. Bioin<or$atics 7<4fo "- )nglan"8- ,. =9- n. =1- 1. =897-=899. =009. S.+NN<N- &. CDtosca1$B + Softwa $ )n,i on%$nt fo Int$g at$" *o"$ls of #io%ol$c(la Int$ action N$two Vs. Geno$e #esearch- ,. 13- n. 11- 1. =498b=904. =003. S]W;+?C]@W- D.> '?+NC)SC.INI- +.> WL.N- *.> SI*<N<FIC- *.> ?<I.- +.> *INCL)]- &.> D<)?WS- I. $t al. I0$ SI?INC "atabas$ in =011B f(nctional int$ action n$two Vs of 1 ot$ins- globallD int$g at$" an" sco $". 8ucleic !cids #esearch- ,. 39- n. 1- 1. D961-D968. =011. II+N- C.> #))S<N- ^. I.> I+F+?<N)- +. I.> SLN- Y.> *+?;)II+- *. +-> C+I)- Y. .. D.> C;+SS- N. ;. SDst$%s analDsis of 1lant c$ll wall "$g a"ation bD t0$ %o"$l fila%$nto(s f(ng(s N$( os1o a c assa. &roceedings o< the 8ational !cade$2 o< Sciences o< the United States o< !$erica- ,. 106- n. 9=- 1. ==197b==16= . =009. FISS)?- Y. +n .D1ot0$sisB I0$ Sa%$ Si4 &olDsacc0a i"$s a $ Co%1on$nts of t0$ & i%a D C$ll ^alls of +ll .ig0$ &lants. &rogress in Biotechnolog2- ,. 14- 1. 47b99. 1996. @L- ..> WI*- &. *.> S&?)C.)?- ).> I?I'<N<F- F.> C)?SI)IN- *. I0$ i%1o tanc$ of bottl$n$cVs in 1 ot$in n$two VsB co $lation wit0 g$n$ $ss$ntialitD an" $41 $ssion "Dna%ics. &;oS co$putational "iolog2- ,. 3- n. 4- $99. =007.

Di3logos 4 Ci5ncia, no 00, $ar6o de /*)0

#ede de ensino '7C