Nikon C2 Confocal Stup Manual

CONFOCAL MICROSCOPE C2/C2si
<SETUP>

This manual is intended to provide setup instructions for Nikon
representatives who have attended the lecture on laser safety and have
been trained in setup operations. Attempts by others to set up the product
may lead to accidents or equipment failure. NIKON is not responsible for
personal injury or equipment damage resulting from equipment setup
performed by unqualified persons.

Be sure to observe the warnings and cautions described in this manual to
ensure safety during setup.

M547E 13.7.Nx.3
WARNING
[Confidential]

i
Introduction

This setup manual is written for service personnel who is installing the Nikon Confocal Microscope C2/C2si.
To ensure correct installation, read this manual carefully before installing this product.
No part of this manual may be reproduced or transmitted in any form without prior written permission from
Nikon.
The contents of this manual are subject to change without notice.
The equipment described in this manual may differ from the actual product in its appearance
This manual contains confidential information. Do not disclose this manual to a third party other than the
authorized Nikon personnel.
Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may
remain. If you note any points that are unclear or incorrect, please contact a Nikon Service Department.
Some of the equipment described in this manual may not be included in the set you are installing.
If you intend to use any other equipment with this product, read the manual for that equipment too.
If this equipment is used in a manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.
Microsoft, Windows, and Internet Explorer are registered trademarks of Microsoft Corporation in the U.S. and
other countries. Other product and company names mentioned in this manual are trademarks or registered
trademarks of their respective owners.

ii
Contents
Introduction ........................................................................................................................................................... i
Safety Precautions ............................................................................................................................................... vi
WARNING and CAUTION Symbols ................................................................................................. vi
WARNING ................................................................................................................................. vii
CAUTION ................................................................................................................................... ix
Chapter 1 System Configuration .................................................................................................................... 1
1.1 System ................................................................................................................................... 1
1.2 System Configuration ............................................................................................................ 2
Chapter 2 Laser Safety .................................................................................................................................... 9
2.1 Laser Paths ............................................................................................................................ 9
2.1.1 Laser Units............................................................................................................... 9
2.1.2 Scan Head ............................................................................................................. 10
2.1.3 Spectral Detector ................................................................................................... 12
2.1.4 Detector Unit .......................................................................................................... 13
2.1.5 Upright Microscope ................................................................................................ 14
2.1.6 Inverted Microscope .............................................................................................. 15
2.2 Safety Labels and Components with Apertures ................................................................... 16
2.2.1 Controller ............................................................................................................... 17
2.2.2 Scan Head and Microscope 80i/90i with DIH ......................................................... 18
2.2.3 Scan Head and Microscope 80i/90i with U-Epi ...................................................... 20
2.2.4 Scan Head and Microscope Ni-E/U [with Trinocular Tube] .................................... 22
2.2.5 Scan Head and Microscope Ni-E/U [with Quadrocular Tilting Tube] ...................... 24
2.2.6 Scan Head and Microscope FN1 [with C-TT-C Trinocular Tube T-C and NI-FL] ... 26
2.2.7 Scan Head and Microscope FN1 [with C1 Y-TT Trinocular Tube and U-Epi] ........ 28
2.2.8 Scan Head and Microscope FN1 [with Quadrocular Tilting Tube] ......................... 30
2.2.9 Scan Head and Microscope AZ100 ....................................................................... 32
2.2.10 Scan Head and Microscope Ti-E/U ........................................................................ 34
2.2.11 C-LU3EX 3 Laser Unit ........................................................................................... 36
2.2.12 LU4A 4 Laser Unit A .............................................................................................. 37
2.2.13 LU-LR 4 Laser PS Rack ........................................................................................ 38
2.2.14 C2-DU3 Detector Unit T ......................................................................................... 39
2.2.15 C2-DUS Spectral Detector Unit ............................................................................. 40
2.2.16 C2-DUT Diascopic Detector Unit ........................................................................... 41
Chapter 3 Part Descriptions .......................................................................................................................... 42
3.1 Scan Head ........................................................................................................................... 42
3.2 Controller ............................................................................................................................. 45
3.3 Laser Unit ............................................................................................................................ 48
3.3.1 C-LU3EX 3 Laser Unit and C1-AOM AOM Unit ..................................................... 48
3.3.2 LU4A 4 Laser Unit A .............................................................................................. 50
3.3.3 LU-LR 4 Laser PS Rack 100V/120V/230V ............................................................ 53
3.4 Detector ............................................................................................................................... 55
3.4.1 C2-DU3 Detector Unit T ......................................................................................... 55
3.4.2 C2-DUT Diascopic Detector Unit ........................................................................... 57
Contents
iii
3.4.3 C2-DUS Spectral Detector Unit ............................................................................. 58
3.5 Microscope .......................................................................................................................... 59
3.5.1 DIH ........................................................................................................................ 61
3.5.2 C-TT-C Trinocular Tube T-C or C1 Y-TT Trinocular Tube ..................................... 61
3.5.3 NI-TT Quadrocular Tilting Tube ............................................................................. 62
3.5.4 NI-TT-E Motorized Quadrocular Tilting Tube ......................................................... 62
3.5.5 NI-AST Analyzer Slider Stopper ............................................................................ 63
3.5.6 AZ-TE100LS Ergonomic Trinocular Tube 100 LS .................................................. 63
3.5.7 Laser Safety Kit for Ti-E/U ..................................................................................... 64
Chapter 4 Preparations .................................................................................................................................. 65
4.1 Checking Items and Tools ................................................................................................... 65
4.1.1 Required Items ...................................................................................................... 65
4.1.2 Tools ...................................................................................................................... 65
4.2 Setup Work Procedure ........................................................................................................ 67
Chapter 5 Setting up Microscopes ............................................................................................................... 69
5.1 Setting up Microscope 80i/90i with DIH ............................................................................... 70
5.2 Setting up Microscope 80i/90i with U-Epi............................................................................. 72
5.3 Setting up Microscope Ni-E/U with Trinocular Tube ............................................................ 73
5.4 Setting up Microscope Ni-E/U with Quadrocular Tilting Tube .............................................. 76
5.5 Setting up Microscope FN1 with Trinocular Tube ................................................................ 79
5.6 Setting up Microscope FN1 with Quadrocular Tilting Tube .................................................. 82
5.7 Setting up Microscope AZ100 .............................................................................................. 85
5.8 Setting up Microscope Ti-E/U .............................................................................................. 86
Chapter 6 Setting up Scan Head ................................................................................................................... 91
6.1 Preparation .......................................................................................................................... 91
6.2 Attaching Ring Adapter ........................................................................................................ 92
6.3 Fixing Scan Head in Place ................................................................................................... 94
6.3.1 Microscope 80i/90i ................................................................................................. 94
6.3.2 Microscope Ni-E/U ................................................................................................. 95
6.3.3 Microscope FN1 ..................................................................................................... 96
6.3.4 Microscope AZ100 ................................................................................................. 97
6.3.5 Microscope Ti-E/U ................................................................................................. 98
6.4 Inserting 1st Dichroic Mirror ................................................................................................. 99
6.5 Connecting Optical Fiber Cables ....................................................................................... 100
Chapter 7 Setting up Diascopic Detector ................................................................................................... 102
7.1 Preparation ........................................................................................................................ 102
7.2 Attaching Optical Path Selector Part .................................................................................. 103
7.2.1 Microscope 80i/90i, Ni-E/U, or FN1 ..................................................................... 104
7.2.2 Microscope AZ100 ............................................................................................... 105
7.2.3 Microscope Ti-E/U ............................................................................................... 109
7.3 Attaching Diascopic PMT ................................................................................................... 111
7.4 Attaching/Connecting Light Guide Fiber ............................................................................ 111

Contents
iv
Chapter 8 Setting up Detector Unit ............................................................................................................. 112
8.1 Preparation ........................................................................................................................ 112
8.2 Inserting Filter Cubes (Optional) ........................................................................................ 113
8.3 Connecting Optical Fiber ................................................................................................... 115
8.4 Attaching Diascopic PMT (MHE47300) ............................................................................. 117
Chapter 9 Setting up Spectral Detector ..................................................................................................... 118
9.1 Preparation ........................................................................................................................ 118
9.2 Releasing Optical Fiber ..................................................................................................... 119
9.3 Installing Spectral Board .................................................................................................... 120
9.4 Connecting Optical Fiber ................................................................................................... 121
Chapter 10 Setting up 4 Laser PS Rack ....................................................................................................... 122
10.1 Preparation ........................................................................................................................ 122
10.1.1 Mountable Laser Power Supplies ........................................................................ 122
10.1.2 Components (Accessories Included) ................................................................... 123
10.2 Mounting Laser Power Supplies ........................................................................................ 125
10.2.1 Power Supplies Mounted ..................................................................................... 125
10.2.2 Positions of Laser Power Supplies ...................................................................... 126
10.2.3 Mounting Procedure ............................................................................................ 129
10.3 Details on Mounting Laser Power Supplies ....................................................................... 131
10.3.1 Mounting Laser Diodes (56RCS Series) .............................................................. 131
10.3.2 Mounting Ar Lasers (IMA Series) ......................................................................... 132
10.3.3 Mounting DPSS Lasers (85BCD/85YCA Series) ................................................. 133
10.3.4 Mounting HeNe Laser (05LGP Series) ................................................................ 134
Chapter 11 Setting up 3 Laser Unit EX ......................................................................................................... 135
11.1 Preparation ........................................................................................................................ 136
11.2 Laser Installation ................................................................................................................ 140
11.3 Laser Rough Adjustment 1 ................................................................................................ 146
11.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber ........ 163
11.6 Final Adjustment for Transmitting HeNe Laser and 405-nm Laser Light through
Single-mode Optical Fiber ................................................................................................. 168
11.7 Sheet Metal Cover Attachment .......................................................................................... 170
Chapter 12 Setting up 4 Laser Unit A ........................................................................................................... 172
12.1 Preparation ........................................................................................................................ 173
12.2 Laser Installation ................................................................................................................ 177
12.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber ........ 200
12.6 Final Adjustment for Transmitting Other Laser Light through Single-mode Optical Fiber .. 205
12.7 AOTF Driver Remote Controller Detachment .................................................................... 207
12.8 Operation Check ................................................................................................................ 208
12.9 Sheet Metal Cover Attachment .......................................................................................... 209

Contents
v
Chapter 13 Setting up Controller .................................................................................................................. 210
13.1 Installing Spectral Board (Optional) ................................................................................... 210
Chapter 14 Setting up PC and Software ....................................................................................................... 212
14.1 D/A Board Installation [Only when AOM Unit or 4 Laser Unit A Is Used] ........................... 212
14.2 Network Settings ................................................................................................................ 213
14.3 Software Installation .......................................................................................................... 215
14.3.1 Application Software ............................................................................................ 215
14.3.2 Setup Tool Software ............................................................................................ 215
14.4 Setup ................................................................................................................................. 216
14.4.1 Launching C2SetupTool ...................................................................................... 216
14.4.2 Hardware Configuration Setting ........................................................................... 217
14.4.3 Laser Information Setting ..................................................................................... 218
14.4.4 Scan Setting ........................................................................................................ 222
14.4.5 Confirmation Screen ............................................................................................ 223
14.5 System Adjustment ............................................................................................................ 224
14.5.1 Starting C2CalibrationTool ................................................................................... 224
14.5.2 Checking/Modifying/Creating Device Control Parameter Setting ......................... 225
14.5.3 Checking/Modifying/Creating Laser Calibration Setting ....................................... 226
14.6 System Simple Operation .................................................................................................. 227
14.6.1 [Live] Tab: Image Capturing Tool ......................................................................... 227
14.6.2 [Laser Power] Tab: Controlling Laser Power ....................................................... 228
14.6.3 [Detail Setting] Tab: Checking/Setting Details of XML File .................................. 229
Chapter 15 Cable Connections between Units ............................................................................................ 232
Chapter 16 General Adjustment .................................................................................................................... 237
16.1 Adjustment of Scan Head Inclination ................................................................................. 238
16.1.1 Microscope 80i/90i, Ni-E/U, or FN1 ..................................................................... 238
16.1.2 Microscope AZ100 ............................................................................................... 241
16.1.3 Microscope Ti-E/U ............................................................................................... 242
16.2 Adjustment of Condenser Lens in Front of Pinhole ............................................................ 245
16.3 Compensation of Image Offset for Bidirectional Scanning ................................................. 247
16.4 Microscope Settings for Diascopic DIC Microscopy ........................................................... 250
16.5 Accuracy Verification of Spectral Detector ......................................................................... 252
Chapter 17 Confirmation and Check Sheet Entry .......................................................................................... 255
Chapter 18 Troubleshooting ......................................................................................................................... 260
Chapter 19 Specifications ............................................................................................................................. 262

vi
Safety Precautions

To ensure correct and safe operation, read this manual before using this product.

WARNING and CAUTION Symbols
Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure
to follow the safety instructions provided may cause personal injury or property damage. To ensure correct
installation, read this setup manual carefully and thoroughly before installing this product.
Safety instructions in this manual are marked with the following symbols to indicate their importance. For your
safety, always follow the instructions marked with these symbols.
Symbol Description
WARNING
Disregarding instructions marked with this symbol may lead to serious injury or
death.
CAUTION
Disregarding instructions marked with this symbol may lead to injury or property
damage.

Safety Precautions

vii
! WARNING

WARNING

Use of controls or adjustments or performance of procedures other than those specified in this
manual may result in hazardous radiation exposure.
1. Laser safety
This laser product is designed and built in compliance with the Performance Standards for
Light-Emitting Products established by the U.S. FDA and the IEC Laser Product Safety Standard
(IEC 60825-1:2007), except for deviations pursuant to Laser Notice No.50. Users are requested to
take all appropriate safety measures specified by the preceding standards, in accordance with local
laws and regulations. Use of this laser product must be supervised by a laser safety officer (LSO)
and users must follow the LSOs instructions.

IEC Class 3B Laser Product
Wavelength: 400 to 700 nm
(Visible CW (Continuous Wave) laser)
Beam divergence: 0.26 to 2.74 rad
Maximum power or energy output:
Total power of 500 mW Max. (Visible CW laser)
2. Intended product use
This product is used only for microscopic observations. It must be attached to the specified
microscope model. Do not use this product for any other purpose.
3. Microscopes and mounting
This product must be mounted to a specified microscope following the mounting procedure below.
Failure to do so may result in blindness from inadvertent exposure to laser light emitted from the
eyepiece section.
Specified microscope Products required for installation
Upright
microscope
80i/90i
DIH + C1 TE mount
C-TT-C trinocular tube T-C
C1 Y-TT trinocular tube
Ni-E/U
NI-TT quadrocular tilting tube
C2-NI-TT quadrocular tilting tube adapter
NI-TT-E motorized quadrocular tilting tube
FN1
AZ100
AZ-TE100LS ergonomic trinocular tube 100LS
AZ 100 stage cover
Inverted
microscope
Ti-E/U with laser safety kit Left side port through C1-TI TI mounting adapter

Co mp l i e s wi t h F DA p e r f o r ma n c e s t a n d a r d s
f o r l a s e r p r o d u c t s ex c e p t f o r
d ev i a t i o n s p u r s u a n t t o L a s e r No t i c e
No. 5 0 , d a t e d J u n e 2 4 , 2 0 0 7 .
LASER RADIATION
AVOID EXPOSURE TO BEAM
CLASS 3B LASER PRODUCT
Total Power 500mW MAX.
CW 400~700nm
IEC/ EN60825-1: 2007
Safety Precautions

viii

WARNING

4. Pre-operation check items
Before using the system, always confirm the following:
An objective or a cap is in place on each of the nosepiece sockets.
A dia-illumination lamphouse or an epi-illumination lamphouse is attached.
There are no mirrors or other highly reflective pieces of metal in the area surrounding the
lens aperture.
The provided cap is in place on the rear port of the quadrocular tube when the upright
microscope Ni-E/U or FN1 is used without the DSC zooming port for quadrocular tube.
A camera or a provided cap is in place on the camera port when the upright microscope
FN1 is used with the FN-DP double port.
A cap or a camera is in place on the right side port when the inverted microscope Ti-E/U is
used.
5. Never attempt to disassemble
This product uses lasers. To ensure safety, never attempt to remove any part of the system. Before
beginning operations, confirm that all of the parts listed below are in place. Removal of any part
during use may result in unintended exposure of eyes or skin to laser light. Be sure to follow the
LSOs instructions when replacing an objective.
Eyepiece tube, nosepiece, objective, epi-fluorescence attachment, stage, lamphouse, scan head, a
cap on the rear port of the quadrocular tube for the upright microscope Ni-E/U or FN1 (when the
dedicated DSC zooming port for quadrocular tube is not used), a cap or a camera mounted on the
FN-DP double port for the upright microscope FN1, and a cap or a camera on the right side port of
the inverted microscope Ti-E/U

6. Do not stare the laser light
When the laser is being fired, a powerful laser light is emitted from the objective. Do not stare the
laser light, and always wear laser-protection goggles when staying within the NOHD (Nominal Ocular
Hazard Distance)
*1
while the laser is fired.
Do not place any reflective objects on the stage or on the optical path of diascopic illumination.
Failure to do so results in exposure of eyes or skin to the hazardous laser light emitted from the
objective and reflected on those objects.
*2

*1: NOHD (Nominal Ocular Hazard Distance): 1.6 m (12.2 m for the AZ100)
*2: Corneal MPE (Maximum Permissible Exposure):
1 W/m
2
(when the visible CW laser of 400 to 700 nm is used)
Skin MPE (Maximum Permissible Exposure):
2000 W/m
2
(when the visible CW laser of 400 to 700 nm is used)
7. Manuals and accessories
All manuals and accessories used in this laser product and provided by the distributor must be
provided to the user.

Safety Precautions

ix
! CAUTION

CAUTION

1. Setting up the system
Setup of this system must be handled by trained service personnel.
Assigning this task to any other personnel may result in improper setup, impairing system
performance and exposing eyes or skin to the laser beam, resulting in injury.
2. Do not disassemble
Disassembling the system may result in electric shock or other hazards.
Never attempt to disassemble any piece of equipment included in the system.
In particular, disassembling any of the components when the laser is on may cause the laser beam
to be improperly emitted from the system. If you discover that the system is not functioning properly,
immediately stop use, switch off the power switches to all pieces of equipment, and disconnect the
power cables from the outlets.
3. Power supply ratings and power supply cords
Before connecting power cords to wall outlets, check your power-line ratings to make sure that the
input voltage and current-carrying capacity requirements of equipment are met. Use of a
non-conforming power line may result in equipment malfunction, failure, or fire. Always use power
cords that satisfy local safety regulations. Always use three-pole grounded outlets. Use of two-pole
outlets may result in electric shock.
4. Cable connections
Always switch off the power switches to all equipment before connecting or disconnecting cables.
To prevent the wires from breaking, do not pull cables by the sheath; instead, firmly grasp the plug
when connecting or disconnecting a cable.
5. Countermeasures against problems
If you notice that the system is not working properly, quickly abort the software on the PC, turn off all
power switches, and disconnect power cords from the wall outlets. If the 4 laser PS rack is abnormal,
unplug the two power cables for it.
6. Prevent contact with moisture
Water splashed onto any component of the system may cause short circuits, resulting in malfunction.
If a piece of equipment is subject to contact with water, immediately switch off the power switch to
that piece of equipment and wipe off the water with a dry cloth. If water enters a component,
immediately suspend use of the system and disconnect the power cord from the outlet.
7. Do not place any objects on system equipment
Never place any objects (heavy ones in particular) on the equipment, except for mounting the laser
unit onto the 4 laser PS rack (given in the next caution item 8). Object can injure the human body
when dropped. A heavy object may cause the deformation, breakage or malfunction of the
equipment.
8. The laser unit and 4 laser PS rack setup
The laser unit is designed to be used on top of the 4 laser PS rack. The laser unit is so heavy that it
can injure the human body when dropped. To avoid injury, be careful not to accidentally push the
laser unit off the 4 laser PS rack.

Safety Precautions

x

CAUTION

9. Handling
1. Handle with care
This system is a precision optical instrument. Make sure that the system is not subject to shock;
handle equipment gently. Also be sure to handle cables with care. Optical-fiber cables, in
particular, must not be bent or pulled with excessive force to prevent failure, malfunction, or break.
2. Installation conditions
To ensure safe and reliable operations, check the following conditions when installing the system:
To prevent equipment from falling and to avoid eventual failure, install each component of the
system on a level surface.
To prevent performance degradation caused by dust or other foreign particles, use the system
in a dirt-free environment.
Use the system in the environment specified in Environmental Conditions of Chapter 19,
Specifications. To prevent condensation and failure, never attempt to use the system under
conditions of high temperature and humidity.
Make sure that the system is not subject to strong vibrations, which may degrade image
quality.
To prevent degradation, malfunction, or failure of your laser units and PC, always use power
supply that is free from electric noise or sudden voltage fluctuations.
To prevent failure, do not block the air flow of the cooling fan and do not place any obstacles
near the cooling fans of the controller, PC, laser units, or 4 laser PS rack. If a particular piece
of equipment is installed close to a wall, be sure to provide at least 15 cm of space between
the wall and the equipment.
3. Transporting
The laser unit and the 4 laser PS rack must be carried with their bottoms securely held by two
or more persons.
Do not push the laser unit when carrying the 4 laser PS rack with laser unit mounted.
Doing so may have laser unit fall off the 4 laser PS rack. Be sure to push the 4 laser PS rack
when transporting.
The 3 laser unit EX weighs approximately 24 kg.
The 4 laser unit A weighs approximately 43 kg.
The 4 laser PS rack weighs approximately 20 kg.
Take care not to get your hand or finger pinched.
4. Cleaning
Use a soft dry cloth to clean painted, plastic, and printed parts. If necessary, wipe these parts with
a cloth moistened with diluted neutral detergent, then wipe off the moisture with a soft dry cloth.
To prevent discoloration or deformation of painted or plastic parts or removal of the printed
letters/figures, do not use organic solvents such as paint thinner or alcohol.
5. Other precautions
Be sure to follow the instructions specified in this manual and in the manual supplied with the
software. Failure to do so may result in impaired system performance, malfunction, failure, or
unexpected hazards.

1

1.1 System

Figure 1.1-1
1 System Configuration
1 System Configuration >> 1.2 System Configuration

2
1.2 System Configuration
Table 1.2-1: System overview (sheet 1 of 3)
Unit Name Code No. Remarks
Scan head &
controller unit
C2-SH C2 scanner and controller MHA43200 Including the controller and optical fiber
C2-SHS C2si Ready scanner and
controller
MHA43300
Including the controller and optical fiber
Available to be set up with the spectral
detector
1st Dichroic
mirrors
(only the model
number of each
filter is shown)
C1 SI-BS 20/80 MHE46900 Select the mirror suitable for the laser.
C1 1STDM 405/488/561 MHE46310
C1 1STDM 408/488/543 MHE46300
C2 1STDM EX405/488/594 MHE46500
C2 1STDM EX440/514/594 MHE46510
C2 1STDM EX 457 (440)/561/640 (633) MHE46520
C2 1STDM EX 457 (440)/543/640 (633) MHE46540
C1 1STDM 405/488/543/640 MHE46410
C1 1STDM 405/488/561/640 MHE46420
C2 1STDM EX440/514/561/640 MHE46530
Detector units C2-DU3 Detector Unit T MHE41400
Fluorescence
filter cube
(only the model
number of each
filter is shown)
C2-F 447/60 MHE46610 Used for 2nd DM
See Table 1.2-4: Recommended filters.
C2-F 482/35 MHE46620
C2-F 494/41 MHE46630
C2-F 510/84 MHE46640
A1-F 515/30 MHE57060
A1-F 525/50 MHE57030
C2-F 537/26 MHE46650
C2-F DAPI/CY5 Dual MHE46660
C2-F 514/30 585/65 MHE46690 Used for 3rd DM
See Table 1.2-4: Recommended filters.
C2-F 525/50 561LP MHE46700
C2-F 525/50 594LP MHE46710
C2-F 537/26 561LP MHE46720
C2-F 550/49 594LP MHE46730
C2-F 585/65 635LP MHE46740
C2-F 593/40 635LP MHE46750
C2-F 515/30 595/50 MHE46760
C2-F 525/50 595/40 MHE46770

3
Diascopic
detector &
adapter
C2-DUT diascopic detector unit MHE47300
C1-TD-J transmit adapter J MHE47700
Used for 80i/90i, Ni-E/U, or FN1
Applicable to A1 and C2
C2-DUT-AZ DUT mounting adapter MHE47800 Used for AZ100
A1-DUT-TI DUT mounting adapter MHE50330
Used for Ti-E/U
Spectral
detector
C2-DUS spectral detector unit MHE43000
Usable only when C2si scan head is
mounted
4 laser unit &
accessories
LU4A 4 laser unit A MHF41402
LU4-C2 connection Kit MHF41412 Required when 4 laser unit A is used
LU-LR 4 laser PS rack 100V MHF44000
3 laser unit &
accessories
C-LU3EX 3 laser unit MHF42305
C1-AOM AOM unit MHE48000
C1-AREX EX changer MHF43000
Switching 488/514 nm
Required when a multiple lines Ar laser is
used
C-LU3EX594 DM set MHV55100
Required only when the 594-nm solid-state
laser is set to L3 position
Laser adapter C1-LAR408 laser adapter R MXA22043 Used for 3 laser unit EX
C1-LAS408 laser adapter S MXA22044 Used for 3 laser unit EX
Laser adapter 405/445/640C MXA22103 Used for 4 laser unit A
C-LAR488 laser adapter MXA22070 Applicable to 3 laser unit EX/4 laser unit A
C-LAR532/561 laser adapter MXA22069 Used for 3 laser unit EX
Laser adapter 561 C MXA22110 Applicable to 3 laser unit EX/4 laser unit A
Laser adapter 594 S MXA22109 Applicable to 3 laser unit EX/4 laser unit A

4
Peripheral
devices for
microscopes
C-RAL ring adapter L MXA22031 Applicable to any microscope
C-RAS ring adapter S MXA22032 Applicable to any microscope
C2-RA C2 ring adapter MHV40000
Applicable to any microscope
(referred as SS in tables)
D-DH-E-A1 digital imaging head for A1 MBD76105 Used for 80i/90i
*1
MHB42200 Used for 80i/90i + U-Epi or FN1
*1
MHB43200 Used for 80i/90i + U-Epi, Ni-E/U, or FN1
U-EPI FL universal epi attachment D-FL MBE74100 Used for 80i/90i or FN1
C1 TE mount MXA22033 Used for 80i/90i + DIH
A1-DIH interlock cable MXA22099
Used for 80i/90i + DIH
NI-TT-E
motorized quadrocular tilting tube
*2

MBB94510
Used for Ni-E
*2
MBB94521 Used for Ni-E/U or FN1
NI-RPZ-E motorized
DSC zooming port for quadrocular tube
*2

MBB96710
Used for Ni-E
NI-RPZ
DSC zooming port for quadrocular tube
*2

MBB96721
Used for Ni-E/U or FN1
NI-FLT6-E
motorized epi-fluorescence cube turret
MBE94300
Used for Ni-E/U
NI-FLT6-I
intelligent epi-fluorescence cube turret
MBE94400
Used for Ni-E/U
NI-FLT6 epi-fluorescence cube turret MBE94500 Used for Ni-E/U or FN1
NI-FLEI epi-fluorescence attachment for Ni MBE94100 Used for Ni-E/U or FN1
NI-BAW-E motorized barrier filter wheel MBE94620 Used for Ni-E
NI-PAU Ni photoactivation unit MBE94800 Used for Ni-E/U
C2-NI-TT quadrocular tilting tube adapter MHV40100 Used for Ni-E/U or FN1 + quadrocular tube
NI-AST analyzer slider stopper
*3
MXA22120 Used for Ni-E/U or FN1
A1-TII interlock cable MXA22094
Used for Ni, FN1 + quadrocular tube, or Ti
FN-DP variable magnification double port MBD75100 Used for FN1
AZ-TE100LS
ergonomic trinocular tube 100LS
MHB43500
Used for AZ100
AZ 100 stage cover MXA22102 Used for AZ100
AZ-FL epi-fluorescence attachment MNE84000 Used for AZ100
C1-TI TI mounting adapter MHV55050 Used for Ti-E/U
Ti laser safety kit MXA22097
Used for Ti-E/U
Software NIS-Elements C MHS50000 Applicable to A1 and C2
*1: Allowable combinations of the upright microscope FN1 and trinocular tube are as follows:
a With the C-TT-C trinocular tube T-C, the NI-FLT6 epi-fluorescence cube turret and the NI-FLEI
epi-fluorescence attachment for Ni are applicable.

5
b With the C1 Y-TT trinocular tube, the U-EPI FL universal epi attachment D-FL is applicable (FN-DP
variable magnification double port can be added).
*2: Allowable combinations of the quadrocular tilting tube and DSC zooming port for quadrocular tube are as
follows:
a With the NI-TT-E motorized quadrocular tilting tube, NI-RPZ DSC zooming port for quadrocular tube or
NI-RPZ-E motorized DSC zooming port for quadrocular tube are applicable.
b With the NI-TT quadrocular tilting tube, NI-RPZ DSC zooming port for quadrocular tube only is
applicable.
*3: NI-AST analyzer slider stopper is necessary for the combinations shown below:
a When any of the NI-FLT6 epi-fluorescence cube turret, NI-FLT6-E motorized epi-fluorescence cube
turret, or NI-FLT6-I intelligent epi-fluorescence cube turret is used with the upright microscope Ni-E/U
b When the NI-FLT6 epi-fluorescence cube turret is used with the upright microscope FN1

Table 1.2-2: Items provided by the distributor
Unit Name Remarks
PC Host PC system The 64-bit version of Windows 7, 1-Gbit Ethernet
LAN cable 1000BASE-T, crossover cable (Cat 5e minimum)
D/A board PCI-6711, PCI-6713, or PCI-6733 (National Instruments made)
Microscopes &
accessories
Microscope
Focus motor Provide the one suitable for the microscope.
Power cables
For C2 controller (x 1)
100 VAC area: 125 VAC 7 A or higher;
200 VAC area: 250 VAC 6 A or higher
For 4 laser unit A (x 1)
For AOM controller (x 1)
For 4 laser PS rack (x 2)
200 VAC area: 250 VAC 6 A or higher, and
For detail, see Chapter 19, Specifications.


6
Table 1.2-3: Recommended lasers
Type Product Specifications
Mounted to
3 laser unit EX
Mounted to
4 laser unit A
CW violet
diode laser
Manufacturer: Melles Griot
Head: 56 RCS/S 2780
Power supply: 56 IMA021
400 to 410 nm
(36 mW)

C1-LAS408 is
required.

Head: 56 RCS/S 2781
Power supply: 56 IMA021
440 to 445 nm
(20 mW)

C1-LAS408 is
required.

Manufacturer: Coherent
Head: CUBE405
Power supply: CUBE series
4055 nm
(50, 100 mW)
---

405/445/640C is
required.
Head: CUBE445
4455 nm
(40 mW)
---

405/445/640C is
required.
Head: RADIUS 405
Power supply: CDRH control box
4055 nm
(25 mW)

C1-LAR408 is
required.
---
CW air-cooled
argon ion laser
Head: IMA 101040 ALS
Power supply: 300 series
457 to 514 nm
(40 mW)

AREX EX changer
is required.

Head: IMA 101065 ALS
457 to 514 nm
(65 mW)

AREX EX changer
is required.

CW blue
solid-state
laser
Head: 85BCD010, 85BCD020, 85BCD030
Power supply: 85BCD series
4880.5 nm
(10, 20, 30 mW)

C-LAR488 is
required.

C-LAR488 is
required.
Head: Sapphire 488 LP
Power supply: Sapphire LP USB CDRH
Driver
4882 nm
(10, 20, 25, 30,
50, 75, 150,
200 mW)

C-LAR488 is
required.

C-LAR488 is
required.
CW green
helium-neon
laser
Head: 05LGP193
Power supply: 05LPL951-065 for 100V
05LPL915-065 for 115/230V
543.5 nm
(1 mW)

CW yellow
solid-state
laser
Head: 85YCA010, 85YCA020, 85YCA025
Power supply: 85YCA series (accessory)
561 nm
(10, 20, 25 mW)

C-LAR532/561 is
required.

Head: Sapphire 561LP-50
Power supply: Sapphire LP USB CDRH
Driver
5602 nm
(50 mW)

561C is
required.

561C is
required.
Manufacturer: Spectra Physics
Head: Excelsior 594 (CDRH model)
Power supply: Excelsior-PS-XC-CDRH
593.70.5 nm
(30, 50 mW)

594S is
required.

594S is
required.

7
Type Product Specifications
Mounted to
3 laser unit EX
Mounted to
4 laser unit A
CW red
helium-neon
laser
Manufacturer: JDS Uniphase
Head: 1125P
Power supply: 1202-3 for 100V,
1202-1 for 115V,
1202-2 for 230V
633 nm
(5 mW)
---
CW red diode
laser
Head: 56RCS/S 2782
Power supply: 56IMA020
640+2/-5 nm
(15 mW)

C1-LAS408 is
required.

Head: CUBE640
640+2/-5 nm
(40 mW)
---

405/445/640C is
required.
Laser diode manufactured by Melles Griot is shown in Nikon specification model (Nikon-dedicated model name).
For the beam divergence and the maximum power, see the manual supplied with each laser.


8
Table 1.2-4: Recommended filters
Excitation laser Filter cube
1st laser 2nd laser 3rd laser 4th laser 2nd DM 3rd DM
408 488 543 ---
C2-F 447/60
(MHE46610)
C2-F 514/30 585/65
(MHE46690)
408 488 561 ---
C2-F 447/60
(MHE46610)
C2-F 525/50 561LP
(MHE46700)
408 488 594 ---
C2-F 447/60
(MHE46610)
C2-F 525/50 594LP
(MHE46710)
440 514 561 ---
C2-F 482/35
(MHE46620)
C2-F 537/26 561LP
(MHE46720)
440 514 594 ---
C2-F 482/35
(MHE46620)
C2-F 550/49 594LP
(MHE46730)
457 543 640 ---
C2-F 494/41
(MHE46630)
C2-F 585/65 635LP
(MHE46740)
457 561 640 ---
C2-F 510/84
(MHE46640)
C2-F 593/40 635LP
(MHE46750)
488 543 640 ---
A1-F 515/30
(MHE57060)
C2-F 585/65 635LP
(MHE46740)
488 561 640 ---
A1-F 525/50
(MHE57030)
C2-F 593/40 635LP
(MHE46750)
514 561 640 ---
C2-F 537/26
(MHE46650)
C2-F 593/40 635LP
(MHE46750)
408 488 543 640
C2-F DAPI/CY5 Dual
(MHE46660)
C2-F 515/30 595/50
(MHE46760)
408 488 561 640
C2-F DAPI/CY5 Dual
(MHE46660)
C2-F 525/50 595/40
(MHE46770)

9

2.1 Laser Paths
2.1.1 Laser Units

4 laser unit A 3 laser unit EX
Figure 2.1-1

(1) L1 (See the table below.)
(5) Motorized shutter
(6) Laser coupling mirror
(7) Laser outlet (single-mode optical fiber)
(8) Continuous brightness adjustment part
(optional AOM unit)
(9) Wavelength selector
(10) AOTF

Table 2.1-1
L1 L2 L3 L4
3 laser unit EX
405-nm laser
440-nm laser
638-nm laser
R-HeNe laser
594-nm solid-state
laser
Ar laser
(488 nm or 514 nm)
Single line or
multiple lines
488-nm solid-state
laser
G-HeNe laser
561-nm solid-state
laser
594-nm solid-state
laser *

4 laser unit A 638-nm laser
405-nm laser
440-nm laser
Ar laser
Single line or
multiple lines
488-nm solid-state
laser
G-HeNe laser
561-nm solid-state
laser
594-nm solid-state
laser
* The laser coupling mirror needs to be replaced with the C-LU3EX594 DM set (MHV55100) when the 594-nm
solid-state laser is set to L3 position on the 3 laser unit EX.
2 Laser Safety
2 Laser Safety >> 2.1 Laser Paths
10
2.1.2 Scan Head
C2 scan head (C2-SH)

Figure 2.1-2

(1) Laser inlet (single-mode optical fiber)
(2) Collimator lens
(3) 1st dichroic mirror
(4) Scanner mirror
(5) Scanner lens
(6) Condenser lens
(7) Pinhole
(8) Fluorescence outlet (multi-mode optical fiber)

11
C2si scan head (C2-SHS)

Figure 2.1-3

(1) Laser inlet (single-mode optical fiber)
(2) Collimator lens
(3) 1st dichroic mirror
(4) Scanner mirror
(5) Scanner lens
(6) Condenser lens
(7) Pinhole
(8) Fluorescence outlet to the detector unit (multi-mode optical fiber)
(9) Fluorescence outlet to the spectral detector (multi-mode optical fiber)

12
2.1.3 Spectral Detector

[Optical path detail]
Figure 2.1-4

(1) Fluorescence inlet into the spectral detector (multi-mode optical fiber)
(2) Collimator lens
(3) Polarized beam splitter
(4) Total reflection mirror
(5) Fresnel rhomb wavelength plate
(6) Diffraction grating
(7) Concave mirror
(8) Optical detector array

(6) (5) (4) (2) (1)
(8)
(7)
(8)
(7)
(7)
(2) (3)
(1)
(4)
(5)
(6)
13
2.1.4 Detector Unit

Figure 2.1-5

(1) Multi-mode optical fiber
(2) Condenser lens
(3) Filter cube (2nd DM)
(4) Filter cube (3rd DM)
(5) Collimator lens
(6) Optical detector (channel 1)

14
2.1.5 Upright Microscope

Figure 2.1-6

Scan head
Laser inlet
Single-mode optical fiber
Multi-mode optical fiber
Spectral detector
Detector unit
Light guide fiber
Laser beam
Optical path selector part of the diascopic detector
15
2.1.6 Inverted Microscope

Figure 2.1-7

Optical path selector part of the diascopic detector Light guide
fiber
Spectral
detector
Detector
unit
Laser inlet
Single-mode
optical fiber
Scan head
Laser beam
2 Laser Safety >> 2.2 Safety Labels and Components with Apertures
16
2.2 Safety Labels and Components with Apertures
(1) IEC warning label
Laser hazard symbol
(2) IEC explanatory label (3) Safety standard label

No. 5 0 , d a t e d J u n e 2 4 , 2 0 0 7 .
LASER RADIATION
CW 400~700nm
IEC/ EN60825-1: 2007

Figure 2.2-1 Figure 2.2-2 Figure 2.2-3
(4) IEC aperture label (5) IEC aperture label (6) IEC caution label
Class 3B areas

AVOID EXPOSURE
LASER LIGHT IS EMITTED
FROM THIS APERTURE

CAUTION CLASS 3B
LASER RADIATION WHEN OPEN
AVOID EXPOSURE TO THE BEAM
VORSICHT LASERSTRAHLUNG
KLASSE 3B,
WENN ABDECKUNG GEFFNET
NICHT DEM STRAHL AUSSETZEN
(7) IEC caution label
Class 3B areas
Class 3B areas
(9) Safety standard label

(10-1) Safety standard label
(100V)*
(120V, 230V)*
Class 4 areas


* These labels are for the 4 laser PS rack. The safety label is different depending on the ratings of your region.

17
2.2.1 Controller
Laser hazard symbol
(2) IEC explanatory label (3) Safety standard label

No. 5 0 , d a t e d J u n e 2 4 , 2 0 0 7 .
LASER RADIATION
CW 400~700nm
IEC/ EN60825-1: 2007


No. 5 0 , d a t e d J u n e 2 4 , 2 0 0 7 .
LASER RADIATION
CW 400~700nm
IEC/ EN60825-1: 2007

Figure 2.2-16

(1)
(2)
(3)
18
2.2.2 Scan Head and Microscope 80i/90i with DIH
Laser hazard symbol
(4) IEC aperture label (6) IEC caution label
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,
Class 3B areas

Figure 2.2-20

Figure 2.2-21

E
A
C
B
(6)
(6)
(1)
D
(4)
(7)
19
A. Component with aperture B. Component with aperture C. Component with aperture

D. Component with aperture E. Head

Figure 2.2-25 Figure 2.2-26

(7)
Aperture
(6)
Aperture
(6)
Aperture
(6)
Aperture
(6)
20
2.2.3 Scan Head and Microscope 80i/90i with U-Epi
Laser hazard symbol
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,
Class 3B areas

Figure 2.2-30

Figure 2.2-31

F
A
D
C
E
B
(6)
(6)
(1)
(4)
(7)
21

D. Component with aperture E. Component with aperture F. Head


(7)
Aperture
(7)
Aperture
(6)
Aperture
(6)
Aperture
(6)
Aperture
(6)
22
2.2.4 Scan Head and Microscope Ni-E/U [with Trinocular Tube]
Class 3B areas
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,

Figure 2.2-41

F
A
D
C
E
B
B
(6)
(4)
(7)
(6)
23



(7)
Aperture

(7)
Aperture Aperture

(6)
Aperture
(6)
Aperture
(6)
(6)
24
2.2.5 Scan Head and Microscope Ni-E/U [with Quadrocular Tilting Tube]
Class 3B areas
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,

Figure 2.2-51

F
A
D
C
E
B
B
(6)
(4)
(7)
(6)
25



(7)
Aperture

(7)
Aperture Aperture

(6)
Aperture
(6)
Aperture
(6)
(6)
26
2.2.6 Scan Head and Microscope FN1 [with C-TT-C Trinocular Tube T-C and NI-FL]
Class 3B areas
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,

Figure 2.2-61

F
A
D
C
E
B
(6)
(4)
(7)
(6)
27



(7)
Aperture

(7)
Aperture Aperture

(6)
Aperture
(6)
Aperture
(6)
(6)
28
2.2.7 Scan Head and Microscope FN1 [with C1 Y-TT Trinocular Tube and U-Epi]
Laser hazard symbol
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,
Class 3B areas

Figure 2.2-71

Figure 2.2-72

F
A
D
C
E
B
B
H
G
(6)
(6)
(1)
(4)
(6)
(6)
(7)
29


G. Component with aperture H. Component with aperture


(7)
Aperture
(7)
Aperture
(6)
Aperture
(6)
Aperture
(6)
Aperture
(6)
Aperture
(6)
(6)
30
2.2.8 Scan Head and Microscope FN1 [with Quadrocular Tilting Tube]
Class 3B areas
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,

Figure 2.2-84

F
A
D
C
E
B
(6)
(4)
(7)
(6)
31



(7)
Aperture

(7)
Aperture Aperture

(6)
Aperture
(6)
Aperture
(6)
(6)
32
2.2.9 Scan Head and Microscope AZ100
Laser hazard symbol
(4) IEC aperture label

Class 3B areas
Class 3B areas
CAUTION CLASS 3B
KLASSE 3B,

Class 3B areas

Figure 2.2-95

Figure 2.2-96

F
A
D
C
B
E
(4)
(1)
On each
objective
(4)
(6)
(6)
(1)
(6)
(7)
(8)
33



(7)
Aperture
(7)
Aperture
(6)
Aperture
(6)
Aperture
(6)
Aperture
(6)
34
2.2.10 Scan Head and Microscope Ti-E/U
Laser hazard symbol
(4) IEC aperture label (5) IEC aperture label

AVOID EXPOSURE
FROM THIS APERTURE

Class 3B areas
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,


*: Affix another pair of these labels on the other side of the microscope.
Figure 2.2-108

C
B A
(6)
(6)
(5)

*
(6)

*
Aperture of analyzer point
(6) (1)
Objective aperture
(6)
Aperture
(4)
(7)
35


(7)
Aperture
Aperture
(6)
Aperture
(6)
36
2.2.11 C-LU3EX 3 Laser Unit
Laser hazard symbol
Class 3B areas


Figure 2.2-114

(1)
(8)
37
2.2.12 LU4A 4 Laser Unit A
Laser hazard symbol
(9) Safety standard label (11) IEC caution label
Class 4 areas


Figure 2.2-118

(1) (11)
(9)
View of Arrow A
A
38
2.2.13 LU-LR 4 Laser PS Rack
(100V)
(120V, 230V)


Front view

Rear view
Figure 2.2-121

(10-1) or (10-2)
39
2.2.14 C2-DU3 Detector Unit T
Class 3B areas
CAUTION CLASS 3B
KLASSE 3B,
Figure 2.2-122

Figure 2.2-123

(6)
(6)
40
2.2.15 C2-DUS Spectral Detector Unit
Class 3B areas
CAUTION CLASS 3B
KLASSE 3B,
Figure 2.2-124

View without the top cover

Figure 2.2-125

Internal cover
(6)
41
2.2.16 C2-DUT Diascopic Detector Unit
Class 3B areas
CAUTION CLASS 3B
KLASSE 3B,
Figure 2.2-126

Figure 2.2-127

(6)
(6)

42

3.1 Scan Head
The scan head is the main optical unit of the system. It is made up of various components, including the
galvano-scanner, pinhole unit, 1st dichroic mirror, and optical system components. The excitation light from the
laser unit is led into this unit through a single-mode optical fiber, and the fluorescence from the specimen is led
into the detector unit through a multi-mode optical fiber.
Two types of the scan head are available; the C2 scan head (MHA43200) and C2si scan head (MHA43300). The
C2si scan head can be combined with the spectral detector, and the transmission of the fluorescence from the
specimen is switchable to the detector unit or spectral detector.

WARNING

Do not remove the scan head from the microscope.
Removing the scan head may cause the laser beam to be emitted through the scan head
mounting hole, resulting in injury.

Front view Side view Rear view
Figure 3.1-1 C2si scan head

(1) FC connector for the laser unit
This connector is used to transmit excitation light (the laser beam) from the laser unit into the scan head.
(2) SMA connector for the detector unit
This connector is used to transmit the fluorescence coming through the pinhole to the detector unit.
(3) FC connector for the spectral detector (only on the C2si scan head)
This connector is used to transmit the fluorescence coming through the pinhole to the spectral detector.

3 Part Descriptions
3 Part Descriptions >> 3.1 Scan Head
43

CAUTION

Do not touch the optical fiber while images are being captured, as doing so may deteriorate the
images.
Do not detach the optical fiber from the scan head to prevent performance degradation and
protect eyes and skin from laser radiation.
Optical-fiber cables, in particular, must not be bent or pulled with excessive force to prevent
breakage.

(4) X connector
Connector for X-axis galvano scanner control
(5) Y connector
Connector for Y-axis galvano scanner control
(6) I/O connector
Connector for the motor control
(7) Laser indicator
The indicator lamp lights up in orange when the laser is ready to emit beam through the objective. The laser
does not emit beam when the indicator lamp is not lit.
(8) 1st dichroic mirror (optional)
The 1st dichroic mirror, which is replaceable, is to
separate the optical paths of excitation light and
fluorescence depending on the excitation wavelength. For
the types of the 1st dichroic mirror, see Table 1.2-1:
System overview in Chapter 1, System Overview.
After 1st dichroic mirror replacement, the condenser lens
may need to be adjusted. For details, see the condenser
lens adjustment screw (11).

Figure 3.1-2

CAUTION

It is an optical component to separate the optical paths of laser beam and fluorescence. If the
glass is not clean, the fluorescence yield lowers, thus do not touch the glass surface. In
addition, damage on the metal part may cause performance deterioration; carefully handle it.
Select the 1st dichroic mirror for the laser (wavelength) used. After 1st dichroic mirror
replacement, be sure to replace the filter cubes in the detector unit suitable for the wavelength.
To use the spectral detector, use the 1st dichroic mirror BS20/80. Other types of 1st dichroic
mirror are also usable, but the spectrums obtained depend on the spectroscopic characteristics
of the 1st dichroic mirror used.

3 Part Descriptions >> 3.1 Scan Head
44
(9) 1st dichroic mirror inlet
Insert the 1st dichroic mirror.

CAUTION

Do not allow any substance other than the 1st dichroic mirror to come into the 1st dichroic
mirror inlet. Otherwise, a failure may occur. If a reflective substance is put in the inlet, the laser
beam may irradiate eyes or skin, and it is dangerous.

(10) Optical path selector lever
Switch the lever to select whether to transmit the
fluorescence from the specimen to the detector unit or
spectral detector. Inside the optical path selector, a
100%-reflection mirror is installed.
Table 3.1-1
Fluorescence detector Lever direction
Detector unit
(filter)
Standard
(slanting)
Spectral detector
(diffraction grating)
Spectrum
(vertical)

(11) Condenser lens adjustment screw
It is placed at the access area for condenser lens
adjustment in front of the pinhole. Although the 1st
dichroic mirror is manufactured with high precision,
pictures may get darkened after replacement or repairing.
For such a case, acquire an image turning this adjustment
screw with the hexagonal screwdriver supplied with the
microscope and make an adjustment so that the center of
the image is the brightest.
If not properly adjusted, fluorescent brightness may
fluctuate during capturing images.
When adjusting, conform to the recommended condition
shown below.
Specimen: Uniform fluorescence specimen
Objective: Magnification of x60 or higher
Pinhole size: S1
Field of view: Maximum

Figure 3.1-3

Adjust the
condenser lens by
turning the screws
3 Part Descriptions >> 3.2 Controller
45
3.2 Controller
The controller is used to control this system and has been adjusted with a set of the scan head.
The controller controls each unit and modulated laser, and generates images. Refer to Chapter 15, Cable
Connections between Units for information on wiring.

CAUTION

Always use power cords that satisfy the appropriate ratings. Make sure your power cords meet
the local safety requirements.

No. 5 0 , d a t e d J u n e 2 4 , 2 0 0 7 .
LASER RADIATION
CW 400~700nm
IEC/ EN60825-1: 2007

Figure 3.2-1 C2 controller

(1) Remote power button
Press the button; the power is turned on, the LED lights in blue, and the controller becomes active. To turn
the power off, press the button while the power is on.
Do not turn the power off while the NIS-Elements C, the operation software for C2, is running.
(2) READY indicator
This indicator lights in green when the C2 controller activates normally.
(3) SCAN indicator
This indicator lights in orange when the Galvano scanner is in operation.
(4) ERR indicator
This indicator lights in red for 1 second when a failure of the C2 controller occurs.
It also lights in red if the system is in the laser interlock status.
(1)
(2)
(3)
(4)
(7)
(6)
(5)
(8)
(9)
(10)
(11)
(12)
(13)
(15)
(18)
(14)
(19)
(17)
(16)
(20)
(21)
(22)
(23)
(25)
(26)
(24)
46
(5) LASER indicator
This indicator lights in orange while a laser is coming into the scan head.
This indicator may fail to light when the incident light intensity is not sufficient.
(6) LU indicator
This indicator lights in blue when communication between the 4 laser unit A and the controller is normal.
(7) DUS indicator
This indicator lights in green when communication between the spectral detector and the controller is
normal.
(8) Detector connector
This connector is used to control the detector unit.
(9) 3EX connector
This connector is used to control the shutters inside the 3 laser unit EX.
(10) AOM connector
This connector is used to control the AOM controller.
(11) X connector
This connector is used to control the X-axis galvano scanner.
(12) Y connector
This connector is used to control the Y-axis galvano scanner.
(13) I/O connector
This connector is used to control the scan head motor.
(14) ILK connector
This connector is used for interlock signals.
(15) 4LU connector
This connector is used to control the 4 laser unit A.
(16) R connector
This connector is used for Ch1 of the detector unit.
(17) B connector
(18) G connector
(19) W connector
This connector is used for ChT (for diascopic detector) of the detector unit.
(20) Spectral board connector (optional)
This connector is used to control the spectral detector.
47
(21) Ext Trg Out connector
This connector is used to output external trigger signals.
(22) Ext Trg In connector
This connector is used to input external trigger signals.
(23) LAN connector
This connector is used for communication with PC. It is 1-Gbit Ethernet compliant.
(24) AC inlet
(25) Power switch
This is the main power switch of the controller. When this switch is on, power is supplied to the primary
source only. Power is not supplied to the secondary source (stand-by state).
(26) Fuse box

3 Part Descriptions >> 3.3 Laser Unit
48
3.3 Laser Unit
3.3.1 C-LU3EX 3 Laser Unit and C1-AOM AOM Unit

CAUTION

The laser unit is designed to be used on top of the 4 laser PS rack. The laser unit is so heavy
that it can injure the human body when dropped. To avoid injury, be careful not to accidentally
push the laser unit off the 4 laser PS rack.

The 3 laser unit EX can accommodate up to 3 lasers. For the accommodatable laser types, see Table 1.2-3:
Recommended lasers in Chapter 1, System Overview. For brightness control, turn the brightness control knob
for the laser or install the optional AOM unit and use software. It has motorized shutters by laser, which can be
opened/closed from the software. For use of the multi-line Ar laser, the optional AREX EX changer allows
selection between 488 nm and 514 nm.

3 laser unit EX AOM controller (optional)
Figure 3.3-1

(1) Laser 1
One laser selected from the 405-nm laser, 440-nm laser, 638-nm laser, R-HeNe laser, or 594-nm solid-state
laser can be attached.
(2) Laser 2
One laser selected from the Ar laser or 488-nm solid-state laser can be attached.
(3) Laser 3
One laser selected from the G-HeNe laser, 561-nm solid-state laser, or 594-nm solid-state laser can be
attached.
(4) Single-mode optical fiber
(2)
(1)
(3)
(4)
(5)
(6)
(7)
(8)
(6)
(6)

(16)
(15)
(14)
(13) (12) (11)
(10)
(9)
49
(5) AOM connector
This connector is used for driving the AOM.
(6) Light control knob (optional AOM)
This knob controls light intensity in 11 discrete steps or
continuously. Transmittance figures obtained when
adjusting an Ar laser is shown in the table below. Laser
beam does not emit when the marker on the knob is in the
unusable area shown in the figure at right. You can also
use the optional AOM unit to control light output.

Figure 3.3-2

Table 3.3-1
Transmittance (%) 100 80 50 25 10 3.5 1.3 0.5 0.15 0.05 0.02

(7) Shutter connector
This connector is used to control the shutters inside this unit.
(8) Ar wavelength selector (optional)
Select one of multi-line Ar laser wavelengths (488 nm or 514 nm).
(9) POWER switch
This is the main power switch of the AOM controller.
When this switch is on, power is supplied to the primary source only. Power supply to the secondary source
is subject to the state of the REMOTE switch (10).
(10) REMOTE switch
This is the remote power switch of the AOM controller.
When this switch is on, power is supplied to the secondary source in conjunction with the power supply to
the PC.
When this switch is off, power is supplied to the secondary source when the POWER switch (9) is on,
independently of the power supply to the PC.
(11) AC inlet
(12) C1-SYNC connector
This connector is used to input Sync signals.
(13) PC connector
This connector is used to control the AOM.
(14) LD-MOD connector (not used)
(15) LD-PWR connector
This connector is used to control power of LD (laser diode).
(16) AOM connector
This connector is used for driving the AOM.

Continuous light
control area
Marker
Step-by-step light
control area
Unusable area
50
3.3.2 LU4A 4 Laser Unit A

CAUTION


The 4 laser unit A can accommodate up to 4 lasers. For the accommodatable laser types, see Table 1.2-3:
Recommended lasers in Chapter 1, System Overview. To adjust laser intensity, control the AOTF with software.
Each laser on this unit is equipped with a software-controlled motorized shutter. Each one of the four orange
LEDs on the front of the unit turns on when the motorized shutter is opened and the corresponding laser is
emitted into the scan head. When the motorized shutter is closed, the corresponding LED is turned off.

Figure 3.3-3

Figure 3.3-4

51
(1) Laser 1
The 638-nm laser can be attached.
(2) Laser 2
One laser selected from the 405-nm laser or 440-nm laser can be attached.
(3) Laser 3
One laser selected from the Ar laser or 488-nm solid-state laser can be attached.
(4) Laser 4
One laser selected from the G-HeNe laser, 561-nm solid-state laser, or 594-nm solid-state laser can be
attached.
(5) Single-mode optical fiber
(6) POWER indicator
This indicator lights in green when the power is turned on.
(7) L1 indicator
This indicator lights in orange when the shutter for laser 1 is opened.
(8) L2 indicator
(9) L3 indicator
(10) L4 indicator
(11) POWER switch
This is the main power switch of the 4 laser unit A.
When this switch is on, power is supplied to the primary source only. Power supply to the secondary source
is subject to the state of the REMOTE switch (13).
(12) AC inlet
(13) REMOTE switch
This is the remote power switch of the 4 laser unit A.
When this switch is on, power is supplied to the secondary source in conjunction with the power supply to
the C2 controller.
When this switch is off, power is supplied to the secondary source when the POWER switch (11) is on,
independently of the power supply to the C2 controller.
(14) AOTF/PC connector
This connector is used to control the AOTF.
(15) CONTROLLER connector
This connector is used to control the 4 laser unit A.
(16) USB connector
This connector is for future expansion. (Do not use in this system.)
52
(17) CONF connector
This connector is used for interlock signals with the controller.
(18) MIC connector
This connector is used for interlock signals with the microscope.
(19) TIRF connector
This connector is intended for extension. It is connected with the shorting pin jack (MHF41412).
When this connector is open, the laser interlock status is set and the shutters cannot be opened.
(20) LD connector
This connector is for future expansion. (Do not use in this system. Otherwise, a malfunction occurs.)
(21) LUSU connector
This connector is intended for extension. It is connected with the rectangular shorting connector
(MHF41412).
When this connector is open, the laser interlock status is set and the shutters cannot be opened.

53
3.3.3 LU-LR 4 Laser PS Rack 100V/120V/230V
The 4 laser PS rack houses the power supplies for lasers mounted on the laser unit, and turns on multiple lasers
or emits all lasers at a time. The laser unit can be mounted on top of the 4 laser PS rack.

Front view

Rear view
Figure 3.3-5

(1)
(2)
(3)
(4)
(5)
(6)
(7)
(8)
(9)
(10)
54
(1) EMISSION laser indicator
This indicator lights in green when the laser emits.
(2) POWER laser indicator
This indicator lights when the power is turned on.
The color varies depending on the system status.
Orange: Only the 4 laser PS rack control system is energized. No laser is turned on (stand-by state).
Green: The 4 laser PS rack control system and all lasers are energized.
(3) EMISSION key switch
This key switch turns on the laser and emits laser.
When the key is in vertical position ( O ): The lasers stop emitting and the key can be extracted.
When the key is in horizontal position ( I ): The lasers start emitting.
(4) Power switch
This is the main power switch of the 4 laser PS rack. When turning on this switch only, no laser is turned on
(stand-by state).
(5) INTERLOCK connector
This connector is used to disable the remote interlock function. When the connector is open, no laser emits
(remote interlock state). To disable the remote interlock function, attach the provided interlock jumper
connector.
(6) Cooling fan
This fan cools the power supply devices in the 4 laser PS rack. When lasers are energized, the fan starts.
(7) Power breaker for 15 A line
This breaker protects over-current of the 4 laser PS rack controller and the Ar laser power supply. Normally
it is turned on.
(8) Power breaker for 5 A line
This breaker protects over-current of the lasers other than Ar laser. Normally it is turned on.
(9) AC inlet for 15 A line
This inlet supplies power to the 4 laser PS rack controller and the Ar laser. When no power is supplied to the
inlet, the 4 laser PS rack does not work.
(10) AC inlet for 5 A line
This inlet supplies power to lasers other than the Ar laser.

CAUTION

Use a power cable that satisfies the power ratings of the AC inlet and the safety standard of the
country.

3 Part Descriptions >> 3.4 Detector
55
3.4 Detector
3.4.1 C2-DU3 Detector Unit T
This unit functions as the optical detector for confocal microscopy.
The fluorescence emitted from the specimen is led into this unit through the objective, scan head, and optical fiber
for fluorescence (multi-mode optical fiber). Set two filter cubes (optional) in the unit to get desired fluorescence
detection from each PMT (photomultiplier).
It is equipped with 3 PMTs, which can serve for simultaneous detection with 3 channels.

Figure 3.4-1

(1) Filter slot cover
Open this cover, and then change the filter cube (optional).
(2)
(3)
(4)
(5)
(6)
(1)
(9)
(7)
(8)
(10)
56
(2) Diascopic detector mounting part
The diascopic detector (optional) is mounted to this part.
(3) T-PMT connector
This connector is used to control the diascopic detector.
(4) CONT connector
This connector is used to control the detector unit.
(5) PMT connector
This connector is used for Ch1 to Ch3, and ChT signals of the PMT.
(6) Multi-mode optical fiber inlet
(7) Filter cube (for 2nd DM) dovetail groove
Insert the filter cube for 2nd DM along with the dovetail groove.
(8) Filter cube (for 3rd DM) dovetail groove
Insert the filter cube for 3rd DM along with the dovetail groove.
(9) Filter cube adapter
Attach the filter cube (10) to the filter cube adapter, and then insert it along with the dovetail groove (7) or
(8).

< How to attach the filter cube to the filter cube adapter >
Insert the filter cube to the filter cube adapter.

Figure 3.4-2
(10) Filter cube (optional)
This filter cube is designed to filter out excitation light and
to separate fluorescence. For the types of the filter cube,
see Table 1.2-1: System overview in Chapter 1, System
Overview.
Also for the combination of the filter cubes, see Table
1.2-4: Recommended filters in Chapter 1, System
Overview.

Figure 3.4-3

Filter
cube
Filter cube
adapter
PMT2
(Ch2)
PMT1
(Ch1)
PMT3
(Ch3)
EM3
Ba2
Ba1
Filter cube
(for 3rd DM)
Filter cube
(for 2nd DM)
57
3.4.2 C2-DUT Diascopic Detector Unit
This is an optional unit to capture transmitted images produced by the laser beam.

WARNING

Do not remove the lamphouse to prevent laser beam radiation escaping from the lamphouse
mount.
Do not touch the optical fiber while images are being captured, as doing so may deteriorate the
images.
Do not detach the optical fiber from the diascopic detector to prevent performance degradation
and protect eyes and skin from laser radiation.
Optical-fiber cables, in particular, must not be bent or pulled with excessive force to prevent
breakage.
Be careful not to touch the adapter while the lamphouse light is on, as the adapter is hot.

Figure 3.4-4

(1) Optical path selector part
Attach the mount adapter to the optical path selector part, and then mount it to the microscope. The mount
adapter varies depending on the microscope.
(2) MOTOR connector
This connector is used to control the optical path selector part.
(3) Light guide fiber (optical fiber)
(4) Light guide fiber inlet

Detector unit
(4)
(1)
(2)
(3)
58
3.4.3 C2-DUS Spectral Detector Unit
This unit is an optical detector to perform spectral observation.
The fluorescence from the specimen is led into this unit through the objective, scan head, and optical fiber for
fluorescence (multi-mode optical fiber).
You can select wavelength resolution pitch from among 2.5 nm, 5 nm, and 10 nm.

Figure 3.4-5

(1) Multi-mode optical fiber
(2) Indicator
This indicator lights in green when the power is turned on.
(3) SIGNAL OUT connector
This connector is used to control the spectral detector.

Calibration
The wavelength and the spectral sensitivity of the spectral detector unit were calibrated with a
reference light source and measurement equipment. And, spectral sensitivities of microscopes,
objectives, and the scan head are calibrated with data.
To keep the calibrated data and to get a correct spectrum, be sure to comply with instructions
below:
(1) Measurement conditions for the spectral detector unit
Warm-up time: approximately 40 minutes
Temperature and humidity of the environment: +18 to +28C, 60%RH or lower (with no condensation)
Measurement wavelength: 420 to 750 nm [applicable wavelength: 400 to 750 nm]
PMT gain: 142 (500 V) to 255 (900 V) [programmable range: 114 (400 V) to 255 (900 V)]
(2) Storage conditions for the spectral detector unit
Storage temperature and humidity: -20 to +35C, 60%RH or lower (with no condensation)
Note: If this system is stored at high temperature, the calibrated data may be adversely affected.

Getting images
When getting images, laser lights reflected from the cover glass or specimen may be detected with
the fluorescent signal. This is not a malfunction.

(2)
(1) (3)
3 Part Descriptions >> 3.5 Microscope
59
3.5 Microscope
This system can be combined and used with any of the microscopes listed below. Install the scan head to the
specified mount on the specified eyepiece tube. Fluorescent device can be used together when the scan head is
installed to the microscope-dedicated trinocular eyepiece tube.
Upright microscopes: 80i/90i, Ni-E/U, FN1, and AZ100
Inverted microscopes: Ti-E/U with the laser safety kit

Table 3.5-1
Microscope
Intermediate
tube/adapter
Mounting
section
FL attachment Ring adapter
Laser safety
device
Upright
microscope
80i/90i
DIH +
C1 TE mount
Front port Built-in L
A1-DIH interlock
cable
C-TT-C trinocular
tube T-C
Camera
port
U-Epi L Not required
C1 Y-TT trinocular
tube
Camera
port
Ni-E/U
(With
Ni-U,
Stage Z
drive only
is
available.)
C-TT-C trinocular
tube T-C
Camera
port
NI-FLT + NI-FLEI
NI-PAU cannot be used.
Focusing
stage
SS
+ S
NI-AST analyzer
slider stopper
NI-TT quadrocular
tilting tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
NI-FLT + NI-FLEI
Focusing
stage
L
A1-TII interlock
cable
NI-AST analyzer
slider stopper
Focusing
nosepiece
S
NI-FLT + NI-FLEI
+ NI-FLT + NI-PAU
When Ni-U is used,
NI-FLT6 + NI-FLEI
+ NI-FLT + NI-PAU
Focusing
stage
SS
Focusing
nosepiece

Ni-E
NI-TT-E motorized
quadrocular tilting
tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
NI-FLT6-I/E + NI-FLEI
Focusing
stage
L
A1-TII interlock
cable
NI-AST analyzer
slider stopper
Focusing
nosepiece
S
+ BA
Focusing
stage
S
Focusing
nosepiece

+ NI-FLT6-I/E + NI-PAU
Focusing
stage
SS
Focusing
nosepiece

60
Microscope
Intermediate
tube/adapter
Mounting
section
Laser safety
device
Upright
microscope
FN1
C-TT-C trinocular
tube T-C
Camera
port
NI-FLT6 + NI-FLEI
S
NI-AST analyzer
slider stopper
C1 Y-TT trinocular
tube
Camera
port
U-Epi + Double port S Not required
NI-TT quadrocular
tilting tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
NI-FLT6 + NI-FLEI
SS + S
A1-TII interlock
cable
NI-AST analyzer
slider stopper
AZ100
AZ-TE100LS
ergonomic
trinocular tube
100LS
Camera
port
AZ-FL S
AZ100 stage
cover
Inverted
microscope
Ti-E/U
with laser
safety kit
C1-TI TI mounting
adapter
Left side
port
Epi-FL
Focusing
stage

A1-TII interlock
cable
Focusing
nosepiece
S

Note 1: The thickness of each ring adapter is as follows:
SS = 2 mm, S = 4 mm, and L = 9 mm.
Note 2: NI-FLT means any of NI-FLT6, NI-FLT6-I, or NI-FLT6-E.

CAUTION

This system must be used with the specified microscope. The scan head must be attached to
the specified mount on the specified eyepiece tube. If this system is used with other microscope
or other settings, laser rays may be emitted from the eyepieces and cause eye injuries in the
laser operation.
This system must be set up by trained service personnel in accordance with the setup manual
instructions.

61
3.5.1 DIH
Either the DIH or the trinocular tube (C-TT-C trinocular tube T-C (MHB43200) or C1 Y-TT trinocular tube
(MHB42200)) described in Section 3.5.2, C-TT-C Trinocular Tube T-C or C1 Y-TT Trinocular Tube is required
when the upright microscope 80i/90 is used in combination with the C2 system. When using the DIH, the interlock
cable (MXA22099) is required to ensure laser safety.

WARNING

Pull out the optical path selector lever on the DIH to avoid the laser radiation instantaneously
from the objective aperture.

Figure 3.5-1

3.5.2 C-TT-C Trinocular Tube T-C or C1 Y-TT Trinocular Tube
These trinocular tubes are laser safety units required when the upright microscope 80i/90i, Ni-E/U, or FN1 is used in
combination with the C2 system. The C-TT-C trinocular tube T-C can be used with the upright microscope 80i/90i,
FN1, or Ni-E, but the C1 Y-TT trinocular tube can be used only with the upright microscope 80i/90i or FN1.
You can use the optical path selector lever to switch between two optical paths: 100% of the light to the binocular
eyepiece and 100% of the light to the vertical tube.

WARNING

Push in the optical path selector lever on the trinocular tube to avoid the laser radiation
instantaneously from the objective aperture.

Optical path selector lever

Figure 3.5-2
100% of the light to
the front port
100% of the light to the
binocular eyepiece
Push in
Push in
Pull out
Pull out
the vertical tube
binocular eyepiece
Pull out
Push in

DIH
Trinocular tube
62
3.5.3 NI-TT Quadrocular Tilting Tube
This quadrocular tilting tube is a laser safety unit required when the upright microscope Ni-E/U or FN1 is used in
combination with the C2 system.
When using this quadrocular tilting tube, the interlock cable (MXA22094) is required to ensure laser safety.

WARNING

Turn and pull out the optical path selector dial on the NI-TT quadrocular tilting tube to avoid the
laser radiation instantaneously from the objective aperture.

Figure 3.5-3

3.5.4 NI-TT-E Motorized Quadrocular Tilting Tube
This quadrocular tilting tube is a laser safety unit required when the upright microscope Ni-E is used in
combination with the C2 system.
When using this quadrocular tilting tube, the interlock cable (MXA22094) is required to ensure laser safety.

WARNING

Switch the optical path in the NI-TT-E motorized quadrocular tilting tube via the optical path
switch to avoid the laser radiation instantaneously from the objective aperture.

Optical path switch
Figure 3.5-4
F: 100% of the light to
the vertical tube
BINO: 100% of the light
to the binocular
eyepiece
FRONT: 100% of the light to
the vertical tube
BINO: 100% of the light to the
binocular eyepiece
63
3.5.5 NI-AST Analyzer Slider Stopper
The NI-AST analyzer slider stopper is composed of the slider stopper and laser safety label.

Figure 3.5-5

3.5.6 AZ-TE100LS Ergonomic Trinocular Tube 100 LS
This laser safety unit is required when using the upright microscope AZ100 in combination with the system. You
can use the optical path selector lever to switch between two optical paths: 100% of the light to the binocular
eyepiece and 100% of the light to the vertical tube.

WARNING

Push in the optical path selector lever on the AZ-TE100LS ergonomic trinocular tube 100LS to
avoid the laser radiation instantaneously from the objective aperture.

Optical path selector lever

Figure 3.5-6

the vertical tube
binocular eyepiece
Pull out
Push in

AZ-TE100LS
Ergonomic
Trinocular Tube
100 LS
Slider stopper
Laser safety label
64
3.5.7 Laser Safety Kit for Ti-E/U
The laser safety kit is composed of the filter slider cover, protection plate for nosepiece, filter cube port cover
retaining plate, and laser safety labels.
LASER RADIATION
AVOID EXPOSURE
FROM OBJECTIVE APERTURE
CAUTION CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM VORSICHT LASERSTRAHLUNG KLASSE 3B, WENN ABDECKUNG GEFFNET NICHT DEM STRAHL AUSSETZEN
AVOID EXPOSURE LASER LIGHT IS EMITTED FROM THIS APERTURE
ON
OFF
6V30W
MAX.
12V100W

Figure 3.5-7

Filter slider cover
Filter cube port cover
retaining plate
Laser safety label
Protection plate for
nosepiece
Laser safety label Laser safety label

65

4.1 Checking Items and Tools
4.1.1 Required Items
Make sure that you have prepared all of the following items before the setup work.
PC system (main unit, monitor, mouse, keyboard, network board, OS)
For details, see Chapter 19, Specifications.
LAN cable: 1000BASE-T, crossover cable (Cat 5e minimum)
D/A board (PCI-6711, PCI-6713, or PCI-6733): Optional (when AOM unit or 4 laser unit A is used)
For details, see Chapter 19, Specifications.
Lasers: Recommended lasers
For details, see Table 1.2-3: Recommended lasers in Chapter 1, System Configuration.
Focus motor: Optional
Provide the one suitable for the microscope.
Power cables:
Cable for the C2 controller (x 1): 100 VAC area: 125 VAC 7 A or higher;
Cable for the 4 laser unit A (x 1): 100 VAC area: 125 VAC 7 A or higher;
Cable for the AOM controller (x 1): 100 VAC area: 125 VAC 7 A or higher;
Cables for the 4 laser PS rack (x 2): 100 VAC area: 125 VAC 7 A or higher;
200 VAC area: 250 VAC 6 A or higher, and

CAUTION

Be sure to prepare power cables satisfying the ratings as specified above and complying with
the local safety standards. Extra care must be taken for the 4 laser PS rack in particular, which
has a great current capacity.
Prepare a LAN cable capable of 1-Gbit communication. A cable for 100-M bit communication
causes reduction of the image frame rate.

4.1.2 Tools
Before starting, make sure that you have prepared the following tools required to set up the C2 system.

General tools
Hexagonal wrench (Allen wrench)
(mm and inch)
A slide of a fluorescent specimen
(or an HE-stained specimen)
Phillips screwdriver
A grid micro specimen

4 Preparations
4 Preparations >> 4.1 Checking Items and Tools
66
Special tools
Laser-protection goggles

Figure 4.1-1
Calibrated power meter

Figure 4.1-2
Power measurement attachment

Figure 4.1-3
Dichroic mirror centering tool (for the EX)

Figure 4.1-4
Coupler centering tool

Figure 4.1-5
Centering tool for pupil observation and a mirror to
observe the centering tool

Figure 4.1-6
Two M4 screwdrivers manufactured by Nikon
(with a red handle)

Figure 4.1-7
Polarization orientation adjustment tool and
polarizer-equipped tool

Figure 4.1-8
Centering tool
* Two pieces are required.

Figure 4.1-9
AOTF driver remote controller (for maintenance)

Figure 4.1-10

4 Preparations >> 4.2 Setup Work Procedure
67
4.2 Setup Work Procedure
1

Microscope setup (Chapter 5)
Microscope setup
Laser safety devices attachment (eyepiece tube, interlock cable,
etc.)
Laser safety labels affixation (WARNING or CAUTION)

2

Scan head setup (Chapter 6)
Ring adapter installation
Installation of the scan head onto the microscope
1st dichroic mirror insertion
Optical fiber connection

3

Diascopic detector setup
(Optional) (Chapter 7)
Attachment of the optical path selector part to the microscope
Attachment of the diascopic PMT to the detector unit
Light guide connection

4

Detector unit setup (Chapter 8)
Filter cubes insertion

5

Spectral detector setup
Attachment of the spectral board to the controller

6

Controller setup (Chapter 13) Power cable connection

7

PC and software setup
(Chapter 14)
D/A board (PCI-6711, PCI-6713, or PCI-6733) installation
[only when the AOM unit or the 4 laser unit A is used]
Network card attachment & network setting
Installation of the software
System setting (using C2SetupTool, C2CalibrationTool, and
C2Tool)

8

Cable connections between
units (Chapter 15)
Cable connections between units

4 Preparations >> 4.2 Setup Work Procedure
68
9

4 laser PS rack setup
Power for the laser connection
Wiring

10
3 laser unit EX setup
(Chapter 11)
or
4 laser unit A setup
(Chapter 12)
Optical axes adjustment of the lasers
AOM or AOTF adjustment (The NIS-Elements needs to be
launched.)

11
General adjustment
(Chapter 16)
Adjustment of the scan head inclination
Adjustment of the condenser lens in front of the pinhole
Compensation of the image offset for the bidirectional scanning
Microscope settings for the diascopic DIC microscopy
[only when the diascopic detector is used]
Accuracy check of the spectral detector
[only when the spectral detector is used]

12
Confirmation and check sheet
entry (Chapter 17)
Laser safety check
Check sheet entry

69

This chapter describes preparations to attach the scan head onto a microscope. Eligible microscopes and related
items are listed in the table below.

WARNING

Do not attempt to attach to microscopes other than those listed below. Doing so may result in
accidental exposure to laser radiation through the eyepiece.
Turn off power supplies to all lasers and units before starting the setup procedures described
here.

Precaution:
Remove the UL mark label affixed on the microscope when 80i/90i, Ni-U, FN1, or Ti-E/U is used.

Table 5.0-1
Microscope
Intermediate
tube/adapter
Mounting
section
Laser safety
device
Upright
microscope
80i/90i
DIH +
C1 TE mount
A1-DIH interlock
cable
C-TT-C trinocular
tube T-C
Camera
port
C1 Y-TT trinocular
tube
Camera
port
Ni-E/U
(With
Ni-U,
Stage Z
drive only
is
available.)
C-TT-C trinocular
tube T-C
Camera
port
NI-FLT + NI-FLEI
Focusing
stage
SS
+ S
NI-AST analyzer
slider stopper
NI-TT quadrocular
tilting tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
NI-FLT + NI-FLEI
Focusing
stage
L
A1-TII interlock
cable
NI-AST analyzer
slider stopper
Focusing
nosepiece
S
NI-FLT + NI-FLEI
+ NI-FLT + NI-PAU
When Ni-U is used,
NI-FLT6 + NI-FLEI
+ NI-FLT + NI-PAU
Focusing
stage
SS
Focusing
nosepiece

Ni-E
NI-TT-E motorized
quadrocular tilting
tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
Focusing
stage
L
A1-TII interlock
cable
NI-AST analyzer
slider stopper
Focusing
nosepiece
S
+ BA
Focusing
stage
S
Focusing
nosepiece

Focusing
stage
SS
Focusing
nosepiece

5 Setting up Microscopes
5 Setting up Microscopes >> 5.1 Setting up Microscope 80i/90i with DIH
70
Microscope
Intermediate
tube/adapter
Mounting
section
Laser safety
device
Upright
microscope
FN1
C-TT-C trinocular
tube T-C
Camera
port
NI-FLT6 + NI-FLEI
S
NI-AST analyzer
slider stopper
C1 Y-TT trinocular
tube
Camera
port
NI-TT quadrocular
tilting tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
NI-FLT6 + NI-FLEI
SS + S
A1-TII interlock
cable
NI-AST analyzer
slider stopper
AZ100
AZ-TE100LS
ergonomic
trinocular tube
100LS
Camera
port
AZ-FL S
AZ100 stage
cover
Inverted
microscope
Ti-E/U
with laser
safety kit
C1-TI TI mounting
adapter
Left side
port
Epi-FL
Focusing
stage

A1-TII interlock
cable
Focusing
nosepiece
S

Note 1: The thickness of each ring adapter is as follows:
SS = 2 mm, S = 4 mm, and L = 9 mm.

5.1 Setting up Microscope 80i/90i with DIH
1. Set up the microscope 80i/90i with DIH.

Figure 5.1-1

5 Setting up Microscopes >> 5.1 Setting up Microscope 80i/90i with DIH
71
2. Connect the interlock cable (MXA22099) to
a) the ILK connector on the DIH and the MIC connector on the 4 laser unit A when the 4 laser
unit A is used; or
b) the ILK connector on the DIH and the ILK connector on the C2 controller when the 4 laser unit
A is not used.

Figure 5.1-2 Rear of the DIH

3. Affix the laser safety labels provided with the C2/C2si (MHA43200/MHA43300) onto the microscope,
as shown in Section 2.2.2, Scan Head and Microscope 80i/90i with DIH in Chapter 2, Laser
Safety.
4. Remove the UL mark label affixed on the rear of the microscope 80i/90i.

Figure 5.1-3

Remove the UL mark label.
Interlock connector on the DIH
5 Setting up Microscopes >> 5.2 Setting up Microscope 80i/90i with U-Epi
72
5.2 Setting up Microscope 80i/90i with U-Epi
1. Set up the microscope 80i/90i and U-Epi.
2. Mount the trinocular tube (MHB43200) or (MHB42200) treated with the laser-safety measures.

Figure 5.2-1

3. As no interlock cable is required for this combination,
a) connect the shorting plug supplied with the C2 (MHA43200/MHA43300) to the MIC connector
on the 4 laser unit A; or
b) connect the shorting plug supplied with the C2 (MHA43200/MHA43300) to the ILK connector
on the C2 controller.
4. Affix the laser safety labels provided with the C2/C2si (MHA43200/MHA43300) onto the microscope,
as shown in Section 2.2.3, Scan Head and Microscope 80i/90i with U-Epi in Chapter 2, Laser
Safety.
5. Remove the UL mark label affixed on the rear of the microscope 80i/90i.

Figure 5.2-2

5 Setting up Microscopes >> 5.3 Setting up Microscope Ni-E/U with Trinocular Tube
73
5.3 Setting up Microscope Ni-E/U with Trinocular Tube
1. Set up the microscope Ni-E/U and the NI-FL.
2. Remove the light shielding part (see Figure 5.3-1) when using any of the NI-FLT6 epi-fluorescence
cube turret, the NI-FLT6-E motorized epi-fluorescence cube turret, or the NI-FLT6-I intelligent
epi-fluorescence cube turret in the system.
1) Remove the cover for inserting filter cubes of the epi-fluorescence cube turret.
2) Remove the light shielding part and fixing screws (two M3 super low head screws) attached in
the turret position 1 from the bottom of the epi-fluorescence cube turret.
Note: Use a 1.5-mm across flats wrench.

Figure 5.3-1 Cover for inserting filter cubes removed
(Dovetail where inserting filter cubes)

3) Attach the cover for inserting filter cubes of the epi-fluorescence cube turret.

CAUTION

Be sure to remove screws fixing the light shielding part. If tightening the screws with the light
shielding part removed, parts may interfere at the rotation of turret resulting in damaging the
epi-fluorescence cube turret in the worst case.

Light shielding part
M3 super low head screws
74
3. Attach the NI-AST analyzer slider stopper.
See the instruction manual supplied with the microscope main body before attaching the NI-AST
analyzer slider stopper.
1) NI-AST analyzer slider stopper
The NI-AST analyzer slider stopper is composed of the slider stopper and laser safety label
(shown below left).
Before using the microscope, check that each item of the NI-AST analyzer slider stopper is
correctly attached (see the figure shown below right).
Affix the laser safety label to the microscope.
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,
Figure 5.3-2

Figure 5.3-3

2) Attach the slider stopper.
Put the slider stopper from the side of the trinocular tube and secure it using the screw
provided with the NI-AST analyzer slider stopper.
4. Mount the trinocular tube (MHB43200) treated with the laser-safety measures.

Figure 5.3-4

Slider stopper
Laser safety
label
75
6. Affix the labels provided with the C2 (MHA43200/MHA43300) onto the microscope as shown in
Section 2.2.4, Scan Head and Microscope Ni-E/U [with Trinocular Tube] in Chapter 2, Laser
Safety.
7. When the microscope Ni-U is used, remove the UL mark label affixed on the microscope.

Figure 5.3-5
5 Setting up Microscopes >> 5.4 Setting up Microscope Ni-E/U with Quadrocular Tilting Tube
76
5.4 Setting up Microscope Ni-E/U with Quadrocular Tilting Tube
1. Set up the microscope Ni-E/U and the quadrocular tilting tube.
2. Remove the light shielding part (see Figure 5.4-1) when using any of the NI-FLT6 epi-fluorescence
cube turret, the NI-FLT6-E motorized epi-fluorescence cube turret, or the NI-FLT6-I intelligent
epi-fluorescence cube turret in the system.



CAUTION


77
(shown below left).
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,
Figure 5.4-2

Figure 5.4-3

Put the slider stopper from the side of the quadrocular tube and secure it using the screw
Slider stopper
Laser safety
label
78
4. When the microscope Ni-U is used, remove the UL mark label affixed on the microscope.

Figure 5.4-4

Section 2.2.5, Scan Head and Microscope Ni-E/U [with Quadrocular Tilting Tube] in Chapter 2,
Laser Safety.
6 Attach the C2-NI-TT quadrocular tilting tube adapter to the quadrocular tilting tube, and then attach
the C2 scan head to the C2-NI-TT quadrocular tilting tube adapter.
a) the INTERLOCK connector on the NI-TT quadrocular tilting tube or the NI-TT-E motorized
quadrocular tilting tube and the MIC connector on the 4 laser unit A when the 4 laser unit A is
used; or
b) the INTERLOCK connector on the NI-TT quadrocular tilting tube or the NI-TT-E motorized
quadrocular tilting tube and the ILK connector on the C2 controller when the 4 laser unit A is
not used.

Figure 5.4-5 Quadrocular tilting tube (Rear bottom on the left part)
5 Setting up Microscopes >> 5.5 Setting up Microscope FN1 with Trinocular Tube
79
5.5 Setting up Microscope FN1 with Trinocular Tube
1. Set up the microscope FN1.
2. Remove the light shielding part (see Figure 5.5-1) when using the NI-FLT6 epi-fluorescence cube
turret in the system.



CAUTION


80
3. When the C-TT-C trinocular tube T-C (MHB43200) is used, attaching the NI-AST analyzer slider
stopper is necessary.
(shown below left).
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,
Figure 5.5-2

Figure 5.5-3

Put the slider stopper from the side of the trinocular tube and secure it using the screw
4. Mount the trinocular tube (MHB43200) or (MHB42200) treated with the laser-safety measures.
Make sure that the notch (index mark) on the vertical tube is positioned on the right side, then,
tighten three setscrews indicated A in Figure 5.5-5
6. Affix the labels provided with the C2 (MHA43200/MHA43300) onto the microscope, as shown in the
diagram in Section 2.2.6, Scan Head and Microscope FN1 [with C-TT-C Trinocular Tube T-C and
NI-FL] or in Section 2.2.7, Scan Head and Microscope FN1 [with C1 Y-TT Trinocular Tube and
U-Epi] in Chapter 2, Laser Safety.
Slider stopper
Laser safety
label
81
7. Remove the UL mark label affixed on the rear of the microscope FN1.

Figure 5.5-4

Figure 5.5-5

Notch (index mark)
Trinocular tube (MHB43200 or MHB42200)
Setscrew A
Vertical tube
5 Setting up Microscopes >> 5.6 Setting up Microscope FN1 with Quadrocular Tilting Tube
82
5.6 Setting up Microscope FN1 with Quadrocular Tilting Tube
1. Set up the microscope FN1 and the quadrocular tilting tube.
2. Remove the light shielding part (see Figure 5.6-1) when using the NI-FLT6 epi-fluorescence cube
turret in the system.



CAUTION


83
(shown below left).
Class 3B areas

CAUTION CLASS 3B
KLASSE 3B,
Figure 5.6-2

Figure 5.6-3

Put the slider stopper from the side of the quadrocular tube and secure it using the screw
Slider stopper
Laser safety
label
84
4. Remove the UL mark label affixed on the microscope FN1.

Figure 5.6-4

Section 2.2.8, Scan Head and Microscope FN1 [with Quadrocular Tilting Tube] in Chapter 2,
Laser Safety.
6 Attach the C2-NI-TT quadrocular tilting tube adapter to the NI-TT quadrocular tilting tube, and then
attach the C2 scan head to the C2-NI-TT quadrocular tilting tube adapter.
a) the INTERLOCK connector on the NI-TT quadrocular tilting tube and the MIC connector on
the 4 laser unit A when the 4 laser unit A is used; or
b) the INTERLOCK connector on the NI-TT quadrocular tilting tube and the ILK connector on the
C2 controller when the 4 laser unit A is not used.

Figure 5.6-5 Quadrocular tilting tube (Rear bottom on the left part)

5 Setting up Microscopes >> 5.7 Setting up Microscope AZ100
85
5.7 Setting up Microscope AZ100
1. Set up the microscope AZ100.
2. Mount the ergonomic tube (MHB43500) treated with the laser-safety measures and the stage cover
(MXA22102).
3. Connect the interlock cable (extending out of the ergonomic tube (MHB43500)) to
a) the MIC connector on the 4 laser unit A when the 4 laser unit A is used; or
b) the ILK connector on the C2 controller when the 4 laser unit A is not used.
4. Affix the labels provided with the C2 (MHA43200/MHA43300) and the stage cover (MXA22102)
onto the microscope, as shown in the diagram in Section 2.2.9, Scan Head and Microscope
AZ100 in Chapter 2, Laser Safety.

Figure 5.7-1

Ergonomic tube
(MHB43500)
Stage cover
(MXA22102)
5 Setting up Microscopes >> 5.8 Setting up Microscope Ti-E/U
86
5.8 Setting up Microscope Ti-E/U
1. Set up the microscope Ti-E/U.
2. Attaching the laser safety kit
Refer to the instruction manual included with the microscope main body before attaching the laser
safety kit.
1) Laser safety kit
The laser safety kit is composed of the filter slider cover, protection plate for nosepiece, filter
cube port cover retaining plate, and laser safety labels shown below (left).
Before using the microscope, check that each part of the laser safety kit is correctly attached.
See the figure shown below (right).
(5) IEC aperture label
LASER RADIATION
AVOID EXPOSURE
FROM OBJECTIVE APERTURE
AVOID EXPOSURE LASER LIGHT IS EMITTED FROM THIS APERTURE
ON
OFF
6V30W
MAX.
12V100W

AVOID EXPOSURE
FROM THIS APERTURE

Figure 5.8-1
Class 3B areas
CAUTION CLASS 3B
KLASSE 3B,
Figure 5.8-2

Figure 5.8-3

2) Attach the filter slider cover to the TI-DH dia pillar illuminator 100W.
Put the filter slider cover from above the dia pillar illuminator and secure it using the screws
and nuts provided with the laser safety kit.
CAUTION CLASS 3B LASER RADIATION WHEN OPEN
AVOID EXPOSURE TO THE BEAM VORSICHT LASERSTRAHLUNG
KLASSE 3B, WENN ABDECKUNG GEFFNET

Figure 5.8-4

Filter slider cover
Filter cube port
cover retaining
plate
Laser safety
label (6)
Protection plate
(for nosepiece)
Laser safety label (5)
Laser safety label (6)
Filter slider cover
Screws and nuts
(on both sides)
87
3) Attach the protection plate for nosepiece between the FL turret and the nosepiece.
[Notes on attaching the nosepiece when the FL turret is used]
The protection plate for nosepiece is a component designed for shielding the gap between the
nosepiece and the Epi-fl filter turret to avoid accidental jamming of fingers inserted into the
gap or the laser radiation from the gap.
Two types of protection plate are prepared for the nosepiece being used. One for the
nosepieces TI-N6, TI-ND6 and TI-ND6-E is supplied with the Ti-E and Ti-E/B, and the other
for the PFS nosepiece TI-ND6-PFS is supplied with the nosepiece. Follow the procedure
below to attach each protection plate.

If the perfect focus system is not used

(1) Remove the two out of the four M3 hexagon
socket head bolts secured on top of the Epi-fl
filter turret (TI-FLC, TI-FLC-E, or TI-FLC-E/HQ),
using the 2-mm hexagonal screwdriver
appended to the Ti-E or Ti-E/B.
(2) Attach the protection plate (for nosepiece)
appended to the Ti-E or Ti-E/B to the top portion
of the Epi-fl filter turret and secure the plate
using the two M3 hexagon socket head bolts
removed in step (1).
(3) Attach the nosepiece (TI-N6, TI-ND6, or
TI-ND6-E) to the Ti-E or Ti-E/B and secure it

Figure 5.8-5

TI-N6 (6 nosepiece),
TI-ND6 (6 DIC nosepiece),
or
TI-ND6-E (motorized DIC
6 nosepiece)
Protection plate
(for nosepiece)
M3 Hexagon
socket head
bolts (x 2)
TI-FLC (FL turret),
TI-FLC-E (motorized FL turret), or
TI-FLC-E/HQ (motorized FL turret HQ)
88
If the perfect focus system is used

(1) Remove the four M3 hexagon socket head bolts
secured on top of the Epi-fl filter turret (TI-FLC,
TI-FLC-E, or TI-FLC-E/HQ), using the 2-mm
hexagonal screwdriver appended to the Ti-E or
Ti-E/B.
(2) Attach the protection plate (for PFS nosepiece)
appended to the PFS nosepiece to the top
portion of the Epi-fl filter turret and secure the
plate using the four M3 hexagon socket head
bolts removed in step (1).
(3) With the cable of the PFS nosepiece being
routed through the cable port of the protection
plate to the Ti-E or Ti-E/B, attach the PFS
nosepiece to the Ti-E or Ti-E/B and secure it

Figure 5.8-6

4) Attach the filter cube port cover retaining plate.
(1) After attaching the filter cube, re-attach the filter cube port cover. (For attaching the filter cube,
see the instruction manual included with the microscope main body.)
(2) Attach the filter cube port cover retaining plate to the side surface of the FL turrets lower plate
using the screws provided with the laser safety kit.

Figure 5.8-7

TI-ND6-PFS
(motorized PFS
6 nosepiece)
M3 Hexagon
socket head
bolts (x 4)
Protection plate
(for PFS nosepiece)
TI-FLC (FL turret),
TI-FLC-E (motorized FL turret), or
TI-FLC-E/HQ (motorized FL turret HQ)
Filter cube port cover
retaining plate
Fixing screw
89
3. Affix the labels provided with the C2 (MHA43200/MHA43300) onto the microscope, as shown in the
diagram in Section 2.2.10, Scan Head and Microscope Ti-E/U in Chapter 2, Laser Safety.
4. Attach the mounting adapter (MHV55050) to the side port.
As shown in the circled figure shown below, align the origin index mark on the straight tube with the
reference index mark and attach the vertical tube to the side port, making sure that the notch (index
mark) faces the front. Then tighten two upper setscrews indicated B.

Figure 5.8-8

a) the INTERLOCK connector on the inverted microscope Ti-E/U and the MIC connector on the
4 laser unit A when the 4 laser unit A is used; or
b) the INTERLOCK connector on the inverted microscope Ti-E/U and the ILK connector on the
C2 controller when the 4 laser unit A is not used.

Figure 5.8-9 Right side of the Ti-E Figure 5.8-10 Rear of the Ti-U

Setscrew B
Reference
index mark
Origin index
mark
INTERLOCK connector INTERLOCK connector
90
6. Remove the UL mark label affixed on the rear of the microscope Ti-E/U.

Ti-E/U
Figure 5.8-11


91

WARNING

Turn off power to all lasers and units before starting the setup procedures described here.
This system must be used with the specified microscope. The scan head must be attached to
the specified mount on the specified eyepiece tube. If this system is used with other microscope
or other settings, laser rays may be emitted from the eyepieces and cause eye injuries in the
laser operation.
This system must be set up by trained service personnel in accordance with the setup manual
instructions.

6.1 Preparation
1

Checking accessories

Confirm that the following accessories have been provided:
Single-mode optical fiber (x 1)
Multi-mode optical fiber (x 1)
Cables (differ from the ones used for C1) (x 3)
X cable
Y cable
I/O cable
Laser safety labels (Aperture label/Caution label) for affixing on the microscope

6 Setting up Scan Head
6 Setting up Scan Head >> 6.2 Attaching Ring Adapter
92
6.2 Attaching Ring Adapter
The scan head must be attached to a microscope. The position of the pupil varies depending on the type of the
microscope or whether the intermediate tube is attached. Thus the ring adapter must be attached to adjust the
position of the pupil. Three types of ring adapters with different heights (L: 9 mm, S: 4 mm, and SS: 2 mm) are
available. They can be attached individually or in combination.
The following table shows the ring adapters used for particular combinations of a microscope and an intermediate
tube.

Table 6.2-1
Microscope
Intermediate
tube/adapter
Mounting
section
Laser safety
device
Upright
microscope
80i/90i
DIH +
C1 TE mount
A1-DIH interlock
cable
C-TT-C trinocular
tube T-C
Camera
port
C1 Y-TT trinocular
tube
Camera
port
Ni-E/U
(With
Ni-U,
Stage Z
drive only
is
available.)
C-TT-C trinocular
tube T-C
Camera
port
NI-FLT + NI-FLEI
Focusing
stage
SS
+ S
NI-AST analyzer
slider stopper
NI-TT quadrocular
tilting tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
NI-FLT + NI-FLEI
Focusing
stage
L
A1-TII interlock
cable
NI-AST analyzer
slider stopper
Focusing
nosepiece
S
NI-FLT + NI-FLEI
+ NI-FLT + NI-PAU
When Ni-U is used,
NI-FLT6 + NI-FLEI
+ NI-FLT + NI-PAU
Focusing
stage
SS
Focusing
nosepiece

Ni-E
NI-TT-E motorized
quadrocular tilting
tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
Focusing
stage
L
A1-TII interlock
cable
NI-AST analyzer
slider stopper
Focusing
nosepiece
S
+ BA
Focusing
stage
S
Focusing
nosepiece

Focusing
stage
SS
Focusing
nosepiece

6 Setting up Scan Head >> 6.2 Attaching Ring Adapter
93
Microscope
Intermediate
tube/adapter
Mounting
section
Laser safety
device
Upright
microscope
FN1
C-TT-C trinocular
tube T-C
Camera
port
NI-FLT6 + NI-FLEI
S
NI-AST analyzer
slider stopper
C1 Y-TT trinocular
tube
Camera
port
NI-TT quadrocular
tilting tube
+ C2-NI-TT
quadrocular tilting
tube adapter
Front port
NI-FLT6 + NI-FLEI
SS + S
A1-TII interlock
cable
NI-AST analyzer
slider stopper
AZ100
AZ-TE100LS
ergonomic
trinocular tube
100LS
Camera
port
AZ-FL S
AZ100 stage
cover
Inverted
microscope
Ti-E/U
with laser
safety kit
C1-TI TI mounting
adapter
Left side
port
Epi-FL
Focusing
stage

A1-TII interlock
cable
Focusing
nosepiece
S


Note:
Always attach the specified size ring adapter for a particular microscope setup. Incorrect attachment
will increase shading (darkening of the peripheral image field).

Remove the relay tube (screw-in type) from the scan head, and then install the ring adapter under the standard
ring.

Figure 6.2-1

Ring adapter (S)
Ring adapter (L) Relay tube
Scan head
Standard ring
6 Setting up Scan Head >> 6.3 Fixing Scan Head in Place
94
6.3 Fixing Scan Head in Place
6.3.1 Microscope 80i/90i

WARNING

Be sure to mount the scan head to the vertical tube of the C-TT-C trinocular tube T-C
(MHB43200) or the C1 Y-TT trinocular tube (MHB42200).
If the scan head is mounted wrongly, laser rays may be emitted from the eyepieces and cause
eye injuries in the laser operation.

Mount the scan head so that the laser indicator is located on the front of the microscope. Set the scan head on
the vertical tube, and then tighten three setscrews indicated C in the figure shown below. Make sure that the ring
adapter is properly inserted in the scan head.

Figure 6.3-1

Setscrew C
Laser indicator
95
6.3.2 Microscope Ni-E/U

WARNING

Be sure to mount the scan head to the vertical tube of the C-TT-C trinocular tube T-C
(MHB43200) or the C2-NI-TT quadrocular tilting tube adapter (MHV40100) attached to the NI-TT
quadrocular tilting tube (MBB94521) (or to the NI-TT-E motorized quadrocular tilting tube
(MBB94510) when the microscope Ni-E is used).


Figure 6.3-2

Setscrew C
Laser indicator
96
6.3.3 Microscope FN1

WARNING

Be sure to mount the scan head to the vertical tube of the trinocular tube (C-TT-C trinocular tube
T-C (MHB43200) or C1 Y-TT trinocular tube (MHB42200)) or to the C2-NI-TT quadrocular tilting
tube adapter (MHV40100) attached to the NI-TT quadrocular tilting tube (MBB94521).


Figure 6.3-3

Setscrew C
Laser indicator
97
6.3.4 Microscope AZ100

WARNING

Be sure to mount the scan head to the vertical tube of the AZ-TE100LS ergonomic trinocular
tube 100LS (MHB43500).

the vertical tube, and then tighten four setscrews. Make sure that the ring adapter is properly inserted in the scan
head.
Adjust the position of the pupil when setting the scan head on the vertical tube by referring Chapter 16, General
Adjustment.

Figure 6.3-4

Laser indicator
Setscrews
98
6.3.5 Microscope Ti-E/U

WARNING

Be sure to install the Ti laser safety kit (MXA22097) when mounting the scan head. If the Ti laser
safety kit (MXA22097) is not installed, laser rays may be emitted from the eyepieces and cause

Mount the scan head to the mounting adapter (MHV55050) attached to the left side port. Mount the scan head so
that the laser indicator is located on the front of the microscope (first dichroic mirror inlet position faces upside.)
Set the scan head on the vertical tube, and then tighten two setscrews indicated C in the figure shown below.
Make sure that the ring adapter is properly inserted in the scan head.

Figure 6.3-5

Setscrew C
Setscrew C Laser indicator
1st dichroic
mirror inlet
6 Setting up Scan Head >> 6.4 Inserting 1st Dichroic Mirror
99
6.4 Inserting 1st Dichroic Mirror
Remove the dummy dichroic mirror from the 1st dichroic mirror inlet. And then, insert the 1st dichroic mirror in
place of it.

Figure 6.4-1

1st dichroic mirror inlet
1st dichroic mirror
6 Setting up Scan Head >> 6.5 Connecting Optical Fiber Cables
100
6.5 Connecting Optical Fiber Cables
1. Detach the sheet metal cover from the back of the scan head.
2. Connect the single-mode optical fiber cable provided with the scan head by aligning its key on the connector
part with the grooved FC connector for the laser unit and screwing the fiber into the place. Connecting the
cable without aligning the key will cause shading. The bent needle jaw pliers shown in Figure 6.5-2 are
useful in screwing the fiber completely.
Make sure that the connector is firmly inserted by pushing/pulling holding the connector part.
The other end of the single-mode optical fiber cable is to be connected to the laser unit after completing
procedures in Chapter 11, Setting up 3 Laser Unit EX or Chapter 12, Setting up 4 Laser Unit A.
3. Connect the SMA connector for the detector unit on the scan head and the detector unit using the
multi-mode optical fiber cable provided with the scan head. For connecting to the detector unit, see Chapter
8, Setting up Detector Unit.
4. For using the C2si scan head with the optional spectral detector, connect the multi-mode optical fiber cable
of the spectral detector to the FC connector for the spectral detector on the C2si scan head. When
connecting, match and connect the key of the FC connector and the key groove. If the key is connected
without matching, images will be extremely dark.
For details of the multi-mode optical fiber cable of the optional spectral detector, see Chapter 9, Setting up
Spectral Detector.
5. Connect the X, Y, and I/O connectors on the scan head and the X, Y, and I/O connectors on the C2
controller using the X, Y, and I/O cables provided with the scan head.

CAUTION

When the multi-mode optical fiber cable for the detector unit or multi-mode optical fiber cable of
the spectral detector is not connected, be sure to attach the provided metal cap onto the
connector. If nothing is attached to the connector, it may result in accidental exposure of eyes
or skin to laser light emitted from the connector.

Figure 6.5-1

Single-mode optical
fiber cable
I/O cable
Y cable
X cable
cable for the detector unit
Sheet metal cover
cable of the spectral
detector
6 Setting up Scan Head >> 6.5 Connecting Optical Fiber Cables
101

Figure 6.5-2 Bent needle jaw pliers

6. For the C2 scan head (MHA43200), attach the fiber bending prevention tube supplied with the product to the
rear of the scan head cover. (For the C2si scan head (MHA43300), this operation is not required.)

Figure 6.5-3

7. Fix the fiber to the electric cable by using the cable clamp so that its bend radius remains around R100 mm
and single-mode optical fiber connecting part is not being bent.

Fiber bending prevention tube
Bend radius is around R100 mm.
Electric cable
Route the fiber along the electric
cable, then fix it with the cable clamp.

102

7.1 Preparation
1

Checking Accessories

Confirm that the following accessories supplied with the diascopic detector (MHE47300) have been
provided:
Optical path selector part (x 1)
Light guide fiber (x 1)
Detector (PMT) (with the light guide fiber inlet) (x 1)
TD-IF cable (x 1)
Cable clamp (x 6)

2

Checking Adapters

The adapter varies depending on the microscope to be used.
80i/90i: C1-TD-J transmit adapter J (MHE47700)
Ni-E/U: C1-TD-J transmit adapter J (MHE47700)
FN1: C1-TD-J transmit adapter J (MHE47700)
AZ100: C2-DUT-AZ DUT mounting adapter (MHE47800)
Ti-E/U: A1-DUT-TI DUT mounting adapter (MHE50330)

7 Setting up Diascopic Detector
7 Setting up Diascopic Detector >> 7.2 Attaching Optical Path Selector Part
103
7.2 Attaching Optical Path Selector Part

WARNING

Before beginning the setup process, be sure to check that the lasers, the controller, and the
microscope are turned off. Note that the laser safety labels affixed on the optical path selector
part warn of potential danger of exposing eyes and skin to the laser beam if any laser is left on
during setup.

Diascopic detector on an upright microscope Diascopic detector on an inverted microscope
Figure 7.2-1

Light guide fiber
Optical path selector
part
Detector unit
Light guide fiber
Optical path selector part
104
7.2.1 Microscope 80i/90i, Ni-E/U, or FN1
1. Detach the diascopic illumination lamphouse from the microscope.
2. Insert the light guide (optical) fiber into the optical path selector part and secure the light guide fiber
with a setscrew.

Figure 7.2-2

3. Shape the light guide fiber in the form of an inverted U with the light guide holder (a sheet metal) on
the side of the optical path selector part.
4. Detach the mounting adapter from the microscope side of the optical path selector part and attach
the cylindrical ring adapter (MHE47700) instead, securing with three setscrews M3 x 8.
5. Attach the ring adapter (MHE47700) with the provided black ring to the lamphouse side of the
optical path selector part, securing with three setscrews M3 x 5.

Figure 7.2-3

6. Insert the optical path selector part into the lamphouse mount on the microscope.
For the FN1, use the FN-LPA lamphouse adapter (MBF72210) to insert the optical path selector
part into the lamphouse mount.
7. Connect the TD-IF cable to the connector of the optical path selector part.
8. Route the TD-IF cable along the light guide fiber, then secure the cable with the provided cable
clamp.
9. Attach the diascopic illumination lamphouse in its place.

M3 x 10 (3 locations)
Light guide fiber
M3 x 8 (3 locations) M3 x 5 (3 locations)
Microscope side Lamphouse side
Remove
C1-TD-J
(MHE47700)
C1-TD-J
(MHE47700)
105
1. Detach the AZ100 light guide fiber for diascopic illumination from the microscope.
with a setscrew.

Figure 7.2-4

4. Remove the fiber guard on the rear of AZ100.
1) Remove the knob of the filter.

Figure 7.2-5

2) Turn the unit upside down, and remove the fiber mount A.

Figure 7.2-6
Light guide fiber

Fiber mount
Fiber guard
106
3) Remove the field diaphragm.
Remove the two screws.

Figure 7.2-7

4) Remove the condenser for low magnification, etc.
Remove the screws (shown below) fixing the condenser. Since the condenser will drop when
the screws are removed, hold the condenser as you remove the screws.

Figure 7.2-8

5) Remove the six screws, and remove the bottom plate. (Remove it ensuring nothing is stuck to
it.)

Figure 7.2-9

Fiber guard
107
6) Remove the two nuts, and remove the fiber guard.

Figure 7.2-10

7) Restore the bottom plate and the condenser, etc. to the original places and secure them.

5. Attach the adapter B of the C2-DUT-AZ DUT mounting adapter (MHE47800) to the microscope
AZ100 using the screws.

Figure 7.2-11

6. Attach the adapter C and D of the C2-DUT-AZ DUT mounting adapter (MHE47800) to the optical
path selector part using the screws.

Figure 7.2-12

Adapter B
Adapter D Adapter C
108
7. Attach the optical path selector part to the adapter B attached to the microscope AZ100 through the
adapter C.
8. Insert the AZ100 light guide fiber for diascopic illumination into the adapter D attached to the optical
path selector part.

Figure 7.2-13

Adapter D
109
1. Detach the diascopic illumination lamphouse from the microscope.
with a setscrew.

Figure 7.2-14

4. Detach the mounting adapter from the microscope side of the optical path selector part and attach
the cylindrical ring adapter (MHE50330) instead, securing with three setscrews M3 x 8.
5. Attach the ring adapter (MHE50330) with the provided black ring to the lamphouse side of the
optical path selector part, securing with three setscrews M3 x 5.

Figure 7.2-15

6. Insert the optical path selector part into the lamphouse mount on the illumination pillar of the
microscope.
7. Connect the TD-IF cable to the connector of the optical path selector part.
8. Route the TD-IF cable along the light guide fiber, then secure the cable with the provided cable
clamp.
9. Secure the light guide fiber and the diascopic illumination lamphouse cable to the illumination pillar
of the microscope with the provided cable clamp.
Light guide fiber
M3 x 10 (3 locations)
M3 x 8 (3 locations) M3 x 8 (3 locations)
Microscope side Lamphouse side
Remove
A1-DUT-TI
(MHE50330)
A1-DUT-TI
(MHE50330)
110
10. Insert the diascopic illumination lamphouse to the rear of the diascopic detector and secure it with
setscrews.

Figure 7.2-16

Figure 7.2-17

TD-IF cable
Light guide fiber
To the detector unit
TD-IF cable Light guide fiber
Light guide holder
Cable clamp
Power cable for the
diascopic illumination
lamphouse
Secure it with the
diascopic illumination
lamphouse cable to the
illumination pillar of the
microscope by using the
cable clamp provided with
the pillar.
7 Setting up Diascopic Detector >> 7.3 Attaching Diascopic PMT
111
7.3 Attaching Diascopic PMT
Attach the diascopic PMT to the detector unit.
For details, see Section 8.4, Attaching Diascopic PMT (MHE47300) in Chapter 8, Setting up Detector Unit.

7.4 Attaching/Connecting Light Guide Fiber

WARNING

Before beginning the setup process, be sure to check that the lasers and controller are turned
off.

Connect the optical path selector part of the diascopic detector and the diascopic PMT with the light guide fiber
and the TD-IF cable.
1. Attach the light guide fiber extending out of the optical path selector part to the detector part
attached to the detector unit. Insert the light guide fiber into the light guide fiber inlet and secure the
light guide fiber with two setscrews.

Figure 7.4-1

2. Connect the TD-IF cable extending out of the optical path selector part to the T-PMT connector on
the detector unit.

PMT
Setscrews for fixing the light guide fiber
(2 places, perpendicular to each other)
Setscrews for fixing the light guide fiber inlet
(2 places, perpendicular to each other) Light guide fiber inlet
Light guide fiber

112

8.1 Preparation
1


Confirm that the following accessories supplied with the detector unit (MHE41400) have been provided:
Cable: (x 2)
3PMT cable
RGBW cable

8 Setting up Detector Unit
8 Setting up Detector Unit >> 8.2 Inserting Filter Cubes (Optional)
113
8.2 Inserting Filter Cubes (Optional)

1. Prepare filter cubes for 2nd DM and 3rd DM
(optional). For the types of the filter cube, see
Table 1.2-1: System overview in Chapter 1,
System Overview.
Also for the combination of the filter cubes, see
Table 1.2-4: Recommended filters in Chapter 1,
System Overview.

Figure 8.2-1

2. Turn the knurled knob on the detector unit and
open the filter slot cover.

3. Pull out the filter cube adapter secured to the
dovetail groove by click in the detector unit.
4. Insert the filter cube to the filter cube adapter.

Figure 8.2-2
Filter cube
Filter cube
adapter
PMT2
(Ch2)
PMT1
(Ch1)
PMT3
(Ch3)
EM3
Ba2
Ba1
Filter cube
(for 3rd DM)
Filter cube
(for 2nd DM)
8 Setting up Detector Unit >> 8.2 Inserting Filter Cubes (Optional)
114

5. Slide in the filter cube attached with the filter
cube adapter along with the dovetail groove in
the detector unit. Be careful with the positions for
2nd DM and 3rd DM.
6. Close the filter slot cover and secure it by turning
the knurled knob.

Figure 8.2-3

Filter cube
attached with
the filter cube
adapter
Knurled knob
2nd DM
position
3rd DM
position
Dovetail
groove
8 Setting up Detector Unit >> 8.3 Connecting Optical Fiber
115
8.3 Connecting Optical Fiber

WARNING

Turn off all lasers and power supplies to perform the setup work.

1. Connecting the multi-mode optical fiber
1) Remove the cover from the detector unit.
2) Remove the cover plate attached on the
multi-mode optical fiber inlet on the rear
panel.
3) Uncap the multi-mode optical fiber supplied
with the scan head, and then route the
optical fiber through the optical fiber inlet
so that the caution label on the fiber is
inside the detector unit.
Figure 8.3-1

Note:
Do not touch the glass section of the multi-mode optical fiber. Staining the glass may degrade
image quality.

4) Securely screw the end of the optical fiber into the receptacle in the detector unit.
5) Reattach the cover plate and the cover.

Figure 8.3-2
CAUTION:
Do not touch the glass
section of the tip.
Cover plate
Multi-mode
optical fiber inlet
Multi-mode
fiber
Receptacle
Caution
label
8 Setting up Detector Unit >> 8.3 Connecting Optical Fiber
116

2. Cable connections
1) Connect the CONT connector on the
detector unit and the Detector connector
on the C2 controller with the supplied
3PMT cable.
2) Connect one end of the supplied RGBW
cable to the PMT connector on the detector
unit, and connect the other ends to the R
and B connectors (on the first line grabber
board) and the G and W connectors (on
the second line grabber board) on the C2
controller.

Figure 8.3-3

CONT connector
PMT connector
8 Setting up Detector Unit >> 8.4 Attaching Diascopic PMT (MHE47300)
117
8.4 Attaching Diascopic PMT (MHE47300)
When the optional diascopic detector (MHE47300) is used, the PMT of the diascopic detector needs to be
attached into the detector unit.
1. Remove the cover from the detector unit.
2. Remove the masking cover on the diascopic PMT mount from the rear panel.

Figure 8.4-1

3. Remove the light guide fiber inlet and attach the diascopic PMT to the rear panel with two screws.
4. Connect the cable extending out of the diascopic PMT to the J4 connector on the 3PMT board in
the detector unit.
5. Reattach the light guide fiber inlet.

Figure 8.4-2

6. Reattach the cover.

Masking cover
Diascopic PMT mount
Diascopic PMT
PMT securing screw
Light guide
fiber inlet
3PMT board

118

WARNING

Turn off all lasers and power supplies to perform the setup work.

Note:
Check item before the setup work
The ambient temperature must be 35C or less. Be sure to check the temperature beforehand.

9.1 Preparation
1


Confirm that the following accessories supplied with the spectral detector (MHE43000) have been
provided:
Cable: (x 2)
I/F cable
12V cable (for connecting inside the controller)
Spectral board (x 1)
Optical fiber cover plate (x 1)

9 Setting up Spectral Detector
9 Setting up Spectral Detector >> 9.2 Releasing Optical Fiber
119
9.2 Releasing Optical Fiber
1. Releasing the multi-mode optical fiber
1) Remove fiber retaining fixtures (four pieces) on the rear panel.
2) Release the optical fiber, then put the fiber retaining fixtures back on the rear panel.

Figure 9.2-1
The other end of the multi-mode optical fiber will be connected to the scan head.

2. Replacing the optical fiber cover plate
1) Remove the cover plate for transportation
from the rear panel.
2) Uncap the multi-mode optical fiber, then
slip the cover plate for transportation off
the optical fiber. (See Figure 9.2-1.)
3) Insert the multi-mode optical fiber through
the opening of the supplied cover plate.
4) Secure the replaced cover plate on the
rear panel.

Figure 9.2-2

Figure 9.2-3

Fiber retaining fixtures
Supplied optical fiber cover plate
Cover plate for transportation
Removed cover plate for transportation
9 Setting up Spectral Detector >> 9.3 Installing Spectral Board
120
9.3 Installing Spectral Board
Attach the spectral board and 12V cable to the C2 controller, referring to Section 13.1, Installing Spectral Board
(Optional) in Chapter 13, Setting up Controller.

9 Setting up Spectral Detector >> 9.4 Connecting Optical Fiber
121
9.4 Connecting Optical Fiber

WARNING

Before beginning the setup process, be sure to check that all lasers and power supplies for the
controller are turned off.

1. Connect the multi-mode optical fiber cable of the spectral detector to the FC connector for the
spectral detector on the C2si scan head. For details, see Section 6.5, Connecting Optical Fiber
Cables in Chapter 6, Setting up Scan Head.
2. Connect the SIGNAL OUT connector on the spectral detector and the spectral board connector on
the C2 controller using the supplied I/F cable.

Figure 9.4-1

SIGNAL OUT connector
Multi-mode optical fiber cable

122

CAUTION

The 4 laser PS rack is heavy. The laser rack weighs approximately 20 kg without laser power
supplies mounted. Do not attempt to lift or carry the laser rack by yourself.
Unplug the two power cables of the 4 laser PS rack before mounting power supplies into the 4
laser PS rack.
Be sure to use the 4 laser PS rack power cables that meet the ratings.
If any trouble occurs in the 4 laser PS rack, unplug the two power cables of the 4 laser PS rack.

10.1 Preparation
10.1.1 Mountable Laser Power Supplies
Table 10.1-1
Violet diode laser Head: 56RCS/S2780
400 to 410 nm/36 mW
Head: 56RCS/S2781
440 to 445 nm/20 mW
Air-cooled argon ion laser Head: IMA101040ALS
457, 477, 488, 514 nm/40 mW
Head: IMA101065ALS
457, 477, 488, 514 nm/65 mW
Blue solid-state laser Head: 85BCD010, 020, 030
488 nm/10, 20, 30 mW
Green helium-neon laser Head: 05LGP193
Power supply: 05LPL951-065 for 100 V;
05LPL915-065 for 115 V/230 V
543 nm/1 mW
Yellow solid-state laser Head: 85YCA010, 020, 025
Power supply: 85YCA series
561 nm/10, 20, 25 mW
Red diode laser Head: 56RCS/S2782
640+2/-5 nm/15 mW

10 Setting up 4 Laser PS Rack
10 Setting up 4 Laser PS Rack >> 10.1 Preparation
123
10.1.2 Components (Accessories Included)
1. 4 laser PS rack main body

Figure 10.1-1

2. Clamps for laser power supplies


[1] For Ar laser [2] For HeNe laser [3] For laser diodes and DPSS
laser

3. Clamp screws

Figure 10.1-5

M4 screw M3 screw

4. Interlock jumper connector

Figure 10.1-6

2 1 4
10 Setting up 4 Laser PS Rack >> 10.1 Preparation
124
5. Keys for the EMISSION switch

Figure 10.1-7

6. Ferrite core

Figure 10.1-8
Figure 10.1-9

[1] For Ar laser cable [2] For Ar laser remote controller
cable

10 Setting up 4 Laser PS Rack >> 10.2 Mounting Laser Power Supplies
125
10.2 Mounting Laser Power Supplies
Mounting positions, clamp screws, and cables vary with the type of laser power supply. Select the clamp positions,
clamp screws, and cables suitable for each laser power supply.

10.2.1 Power Supplies Mounted
Table 10.2-1
Wavelength Power supply
Mounting
position
Clamp Cable Procedure
Violet diode laser Head: 56RCS/S2780
[2] [3] [2] 10.3.1
Head: 56RCS/S2781
[2] [3] [2] 10.3.1
Air-cooled argon
ion laser
Head: IMA101040ALS
[1] [1] [3], [5] 10.3.2
Head: IMA101065ALS
[1] [1] [3], [5] 10.3.2
Blue solid-state
laser
Head: 85BCD010, 020, 030 (CDRH supported)
[4], [5] [3] [6], [7], [9] 10.3.3
Green
helium-neon laser
Head: 05LGP193
Power supply: 05LPL951-065 for 100 V;
05LPL915-065 for 115 V/230V
[3] [2] [4] 10.3.4
Yellow solid-state
laser
Head: 85YCA010, 020, 025
Power supply: 85YCA series
[4], [5] [3] [6], [7], [8] 10.3.3
Red diode laser Head: 56RCS/S2782
[2] [3] [1] 10.3.1

126
10.2.2 Positions of Laser Power Supplies
1. Positions of laser power supplies
(1) Ar, HeNe, Laser diode x 2

Figure 10.2-1

(2) Ar, DPSS, Laser diode x 2

Figure 10.2-2

[1] Ar laser
[3] HeNe laser [2] Laser diode
4 laser PS rack
control box
[1] Ar laser
[5] DPSS laser (power supply) [2] Laser diode
4 laser PS rack
control box
[4] DPSS laser (controller)
Front Rear
Front Rear
127
(3) DPSS, HeNe, Laser diode x 2

Figure 10.2-3

(4) DPSS x 2, Laser diode x 2

Figure 10.2-4

[5] DPSS laser (power supply)
[3] HeNe laser [2] Laser diode
4 laser PS rack
control box
Front Rear
[5] DPSS laser (power supply) [2] Laser diode
4 laser PS rack
control box
Front Rear
[5] DPSS laser (power supply) [4] DPSS laser (controller)
128
2. Positions of cables

Figure 10.2-5

[1] Laser diode power cable
(56ICS-640 nm)
[4] HeNe laser power cable
(HeNe-543 nm)
[2] Laser diode power cable
(56RCS-405 nm, 56RCS-440 nm)
Front Rear
[9] DPSS laser power cable
(85BCD-488 nm)
[3] Ar laser power cable
(IMA Ar Laser)
[8] DPSS laser power cable
(85YCA-561 nm)
[7] RS cable for the DPSS laser
(85BCD/YCA RS232C)
[6] IT cable for the DPSS laser
(85BCD/YCA INTERLOCK)
[5] RC cable for the Ar laser
(IMA REMOTE CONT)
129
10.2.3 Mounting Procedure
1) Remove the side panel, the top panel, and the port panel on the rear side
of the 4 laser PS rack.

2) Mount required laser power supplies into the 4 laser PS rack. For details,
refer to Section 10.3, Details on Mounting Laser Power Supplies.
Set the conditions of all laser heads, the power supplies, and the switches
on the controllers to be ready for laser emission.

Secure all the laser power supplies at the specified positions with the
provided clamps and clamp screws.

Withdraw the cables that connect the laser power supplies and the laser
heads from the cable port on the rear side.
If any of the cables are the same series, make sure that they can be
identified.

Connect the specified cables such as power cables or interfaces to the
laser power supplies and bundle the cables.

3) Bundle unassigned cables and secure them. (See the figures on the next
page.)

4) Attach the removed panels to their original positions.

5) Connect the cables drew from the 4 laser PS rack to each laser.
Attach the provided ferrite core to the Ar laser cable and to the Ar laser
remote controller cable. (See the figures on the next page.)

6) Connect the provided interlock jumper connector to the INTERLOCK
connector on the rear. (See the figure on the next page.)

7) Connect the provided two power cables to the AC inlet of the 4 laser PS
rack. Make sure to use the power cables that meet the ratings indicated
below.
AC inlet (15A type): 125V, 15A (for 100V/120V model),
250V, 10A (for 230V model)
AC inlet (5A type): 125V, 7A (for 100V/120V model),
250V, 6A (for 230V model)

130

Figure 10.2-6

Figure 10.2-7

Figure 10.2-8

INTERLOCK connector
AC inlet (15A type)
AC inlet (5A type)
Cable port
Ferrite core for the Ar
laser cable

Bundle unassigned cables and secure them.
Ar laser remote
controller
Ferrite core for the Ar
laser remote controller
cable
10 Setting up 4 Laser PS Rack >> 10.3 Details on Mounting Laser Power Supplies
131
10.3 Details on Mounting Laser Power Supplies
10.3.1 Mounting Laser Diodes (56RCS Series)
1. Turn on the key switch and the power switches of the laser diode heads located outside the 4 laser
PS rack.
2. Secure the laser diode power supplies in the positions [2] with the provided clamps [3] and clamp
screws.
3. Connect the power cable [1] (56ICS-640 nm) or [2] (56RCS-405 nm, 56RCS-440 nm) to each of the
laser diode power supplies.
4. Pull the cables routed from the laser diode power supplies out of the rack through the cable port on
the rear side. If any of the cables are the same series, mark them so that they can be identified.

Figure 10.3-1

Power cable
(56RCS-405 nm)
(56RCS-440 nm)
(56ICS-640 nm)
To the laser diode head
Laser diode power supplies
Clamp
132
10.3.2 Mounting Ar Lasers (IMA Series)
1. Turn on the key switch and the power switch of the Ar laser power supply. Additionally, fully turn the
POWER ADJUST control of the Ar laser power supply clockwise.
2. Secure the Ar laser power supply in the position [1] with the provided clamps [1] and clamp screws.
3. Connect the power cable [3] (IMA Ar Laser) and the RC cable for the Ar laser [5] (IMA REMOTE
CONT) to the Ar laser power supply.
4. Connect the cable (to be connected to the Ar laser head) to the Ar laser power supply through the
cable port on the rear side.

Figure 10.3-2

5. Connect the cable (to be connected to the Ar laser remote controller) to the 4 laser PS rack control
box located in the rack through the cable port on the rear side.

Figure 10.3-3

Ar laser power supply
Power cable
(IMA Ar Laser)
To the Ar laser head
RC cable for the Ar laser
(IMA REMOTE CONT)
Clamp
Remote controller cable
(to the Ar laser head)
4 laser PS rack control
box
133
10.3.3 Mounting DPSS Lasers (85BCD/85YCA Series)
1. Turn on the key switch of the DPSS laser controller.
2. Secure the DPSS laser controller in the position [4] with the provided clamp screws. (Fix the DPSS
laser controller directly to the bottom plate without clamps.)
3. Secure the DPSS laser power supply in the position [5] with the provided clamps [3] and clamp
screws.
4. Connect the power cable [8] (85YCA-561 nm) or [9] (85BCD-488 nm) to the DPSS laser power
supply.
5. Connect the DPSS laser power cable to the DPSS laser controller.
6. Connect the interlock cable [6] (85BCD/YCA INTERLOCK) to the INTERLOCK port of the DPSS
laser controller.
7. Connect the RS232C cable [7] (85BCD/YCA RS232C) to the RS-232 port of the DPSS laser
controller.
8. Connect the cable (to be connected to the DPSS laser head) to the DPSS laser controller through
the cable port on the rear side.

Figure 10.3-4

To the DPSS laser head
IT cable for the DPSS laser
(85BCD/YCA INTERLOCK)
DPSS laser controller
DPSS laser power
supply
Clamp
Power cable
(85YCA-561 nm)
(85BCD-488 nm)
RS cable for the DPSS
laser
(85BCD/YCA RS232C)
134
10.3.4 Mounting HeNe Laser (05LGP Series)
1. Turn on the key switch of the HeNe laser power supply.
2. Secure the HeNe laser power supply in the position [3] with the provided clamp [2] and clamp
screws.
3. Connect the power cable [4] (HeNe-543 nm) to the HeNe laser power supply.
4. Connect the cable (to be connected to the HeNe laser head) to the HeNe laser power supply
through the cable port on the rear side.

Figure 10.3-5

CAUTION

Mount the HeNe laser power supplies that meet the local power requirements. Misuse of the
power supplies result in damage.

HeNe laser power supply
Clamp
Power cable
(HeNe-543 nm)
To the HeNe laser head

135

WARNING

The unit must be set up as instructed by the local LSO (Laser Safety Officer).
This laser unit uses Class IIIb lasers. Accidental exposure of eyes or skin to laser light may
result in injury or other problems. Do not turn on the lasers until they are installed in the laser
unit.
Confirm that there is no one in the area before turning on the lasers.
Always wear laser-protection goggles while lasers are turned on.
Do not place tools or optical components such as mirrors in the light path while lasers are
turned on.
Use the lowest output power rating during setup. For Ar lasers, use STANDBY mode during
setup.
The laser units are heavy. Without the lasers installed, the 3 laser unit EX weighs approximately
24 kg. Do not attempt to lift the laser unit unassisted.
The laser manual must be provided to the user.

CAUTION

To use a laser diode or a solid-state laser with the spectral detector unit, its wavelength must be
measured with a spectrometer and registered into the C2SetupTool beforehand.

The setup procedure for the 3 laser unit EX is given below. Note that the setup procedure will differ, depending on
whether or not the optional AOM unit (MHE48000) is used.
11.1 Preparation
11.2 Laser Installation
11.3 Laser Rough Adjustment 1
When the AOM unit is used, use the operation tool software C2Tool to manipulate the AOM for laser setup.
Therefore, the system needs to be ready, including PC setup.
11.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber
11.6 Final Adjustment for Transmitting HeNe Laser and 405-nm Laser Light through Single-mode Optical
Fiber
11.7 Sheet Metal Cover Attachment

11 Setting up 3 Laser Unit EX
11 Setting up 3 Laser Unit EX >> 11.1 Preparation
136
11.1 Preparation
1


Confirm that the following accessories supplied with
the 3 laser unit EX (MHF42305) or optional AOM unit
(MHE48000) have been provided:
< 3 laser unit EX >
Cable (x 1)
Hexagonal head screw (x 7)
Harness band (x 6)
Harness band retainer (x 4)
Label (x 1)
Black ring (x 2)

< AOM unit (optional) >
AOM (x 2)
AOM controller (x 1)
Cable (x 5)
AOM cable
RF cable
SYNC cable
PC cable
PCI Bracket cable (not used)

Figure 11.1-1

2

Knob position adjustment

Turn the light control knob (with the marker) to the
MAX position on the step-by-step light control area.

Figure 11.1-2

137
3

Removing the sheet metal cover

Remove the six screws, and then remove the sheet
metal cover.

Figure 11.1-3

4

Attaching the black ring (accessory)

1. Remove the bolts and nuts at three locations of
the laser inlets, holding with fingers to prevent
them from falling.

Figure 11.1-4

2. Attach the black (sponge) ring provided,
centering it at the opening.

Figure 11.1-5

138
5

Removing the fiber coupling

Remove the three external screws, and then remove
the fiber coupling. Handle the coupling carefully to
prevent contamination of the space inside.

Figure 11.1-6

6

Connection

Connect the connector on the 3 laser unit EX and the
3EX connector on the C2 controller using the cable
supplied with the 3 laser unit EX.

Figure 11.1-7

Connector
139
When the optional AOM unit (MHE48000) is used, perform 7 , 8 , and 9 . To manipulate the AOM with the
C2Tool, Chapter 14, Setting up PC and Software and Chapter 15, Cable Connections between Units are to be
performed first.

7

Setting up the PC and D/A board

1. To manipulate the AOM, set up the D/A board (PCI-6711, PCI-6713, or PCI-6733, provided by the
distributer) in the PC. Perform the procedure in Section 14.1, D/A Board Installation [Only When
AOM Unit or 4 Laser Unit A Is Used] in Chapter 14, Setting up PC and Software.

8

Connecting the AOM controller

1. Connect the C1-SYNC connector of the AOM
controller and the AOM connector of the C2
controller using the SYNC cable provided with
the AOM unit.
2. Connect the AOM connector of the AOM
controller and the AOM connecting part of the 3
laser unit EX using the RF cable provided with
the AOM unit.

Figure 11.1-8

3. Connect the PC connector on the AOM controller and the connector on the D/A board of the PC
using the PC cable provided with the AOM unit.

9

Cable connections between units

1. To control the 3 laser unit EX and the AOM unit by the PC, connect cables between units, referring
to Section 15, Cable Connections between Units.

RF cable
PC cable SYNC cable
11 Setting up 3 Laser Unit EX >> 11.2 Laser Installation
140
Table 11.2-1 shows the recommended lasers to be mounted on the 3 laser unit EX.

Figure 11.2-1


Table 11.2-1
L1 L2 L3
3 laser unit EX
405-nm laser
440-nm laser
638-nm laser
R-HeNe laser
594-nm solid-state laser
Ar laser (488 nm or 514 nm)
Single line
Multiple lines
*1

G-HeNe laser
*2
*1: When the multiline Ar laser is in use, the AR changer (MHF43000) needs to be installed prior to the Ar laser.
*2: The laser coupling mirror needs to be replaced with the C-LU3EX594 DM set (MHV55100) when the 594-nm
solid-state laser is set to L3 position on the 3 laser unit EX.

141

Figure 11.2-2

1

Installing the Ar laser

1. Detach the mounting plate from the laser unit.
2. Attach the mounting plate to the Ar laser.
Note that the mounting plate screw positions
differ for the 488 multi-line Ar (black) and 488
single-line Ar (white).
3 front holes: For 488 multi-line Ar
3 rear holes: For 488 single-line Ar

Figure 11.2-3

3. Place the Ar laser at the specified position in the
laser unit. Be sure to press the laser emitter end
against the black ring just enough to compress
the ring to prevent leakage of scattered laser
light. Fix the Ar-laser body temporarily with the
bolts.

Figure 11.2-4

4. Check that the laser head shutter is closed (at the CLOSE position).

Black ring Attach while pressing
against the black ring just
enough to compress it.
HeNe laser, 405-nm laser
(cylindrical type)
Ar laser
142
2

Installing the HeNe laser and 405-nm laser (cylindrical type)

1. Loosen the slot-tightening screw and three
sleeve-retaining screws to allow insertion of the
laser head.

Figure 11.2-5

2. Insert the HeNe laser head into the sleeve. To
prevent leakage of scattered laser light, press
the end of the laser head against the black ring
attached to the plate just enough to compress
the ring slightly. Confirm that the label on the
HeNe laser faces upward when installed.

Figure 11.2-6

3. Installing the 405-nm laser (cylindrical type)
Use the focus adjustment tool provided with
the laser to adjust the laser to give a minimum
beam diameter at 1.5 m to 1.7 m distance
from the emitter.
(This must be performed to increase
transmission efficiency across the fiber
connection and to ensure that the light
controller is operational.)
Insert the 405 laser head into the sleeve,
making sure that the emitter end adapter is
pressed against the plate. Confirm that the
label on the 405 laser faces to the outer side
when installed (elliptic shaped beam is in
vertically oriented).

Figure 11.2-7
143

4. Adjust the positions of the units so that the
sleeve side face aligns with each mount.

Figure 11.2-8

Figure 11.2-9

5. Check that the laser head shutters are closed (at the CLOSE position).
6. Lightly tighten the slot-tightening screw and one of the sleeve-retaining screws loosened in Step 1.

3

Installing the 408 laser (rectangular type)

1. Detach the two cylindrical laser holders.
2. Use the four screws provided to attach the C1-LAR408 laser adapter R (MXA22043).
3. Use the three screws provided to attach the 408 laser (rectangular type).

144
4

Installing the 594-nm solid-state laser

The 594-nm solid-state laser can be installed in L1 or L3.
When installing in L1, the operation in 1 shown below is not required.
When installing in L3, it is necessary to replace the filter with the C-LU3EX594 DM set (MHV55100) as
shown in 1 below.

1. Replace the dichroic set in L1 and L2.

Figure 11.2-10

Replace the L1 and L2 dichroic sets in the 3
laser unit EX and detach the knobs and springs.
Incorporate two dichroic sets of the C-LU3EX594
DM set (MHV55100), and then reattach the
knobs and springs. When incorporating, make
sure the difference between the two glasses that
can be distinguished from one another by their
sizes.

Incorporate in L2
400-515 dichroic set (the one with larger glass)
Figure 11.2-11

Incorporate in L1
400-458 dichroic set (the one with smaller glass)
Figure 11.2-12

2. Attach the laser adapter 594S (MXA22109) as
shown in the figure, and install the 594
solid-state laser.

Figure 11.2-13

Detach and
reattach the knobs
and springs
400-458 dichroic set
(smaller glass)
400-515 dichroic set
(larger glass)
Dichroic face Dichroic mirror
Dichroic face Dichroic mirror
594-nm laser
Excelsior 594
Laser Adapter 594S
(MXA22109)
145
5

Laser warm-up

1. Turn on the lasers.
2. Allow the lasers to warm up for at least 10 minutes.

Note:
Leave the lasers turned on for approximately 10 minutes before adjusting. Making adjustments
immediately after turning on the lasers may result in shifts from adjusted levels.

3. Set the power to minimum (Standby mode) for the Ar laser.

11 Setting up 3 Laser Unit EX >> 11.3 Laser Rough Adjustment 1
146

Figure 11.3-1 Overall view from above

1

Checking the initial position of the beam shift unit

Check that the horizontal setscrew openings on the
sides of the two beam shift units are perpendicular to
the light beam, and that the cylindrical glass section is
not significantly tilted.
(They should be pre-adjusted to these positions
shown in the figure at right before shipment from the
factory.)

Figure 11.3-2

Figure 11.3-3

Light control unit Beam shift unit
Glass section
147
2

Installing the dichroic mirror centering tool (for the EX)

1. Remove the screw, and take off three dichroic
mirror adjustment springs. Take care to keep the
springs from flying off.

Figure 11.3-4

2. Remove two screws (total of 6) on each unit,
then remove three dichroic mirror adjustment
units.

Figure 11.3-5

3

Adjusting the laser installation position

3-1 Adjusting the Ar laser

1. Install the dichroic mirror centering tool (for the
EX) on the Ar laser dichroic mirror unit, making
sure that the tool opening faces in the direction
in which the laser is emitted.

Figure 11.3-6
Spring unit
Dichroic mirror adjustment unit
Dichroic mirror centering
tool (for the EX)
148

2. Open the Ar laser shutter (set to the OPEN
position). Then open the shutter in the Ar laser
optical path on the laser unit side. At this time,
you may insert a hex wrench into the shutter to
keep the shutter open.

Figure 11.3-7

3. Insert a tool into the Ar mirror adjustment hole
and rotate it in order to vertically align the laser
beam and dichroic mirror centering tool opening
(for the EX).

Figure 11.3-8

4. Move the entire Ar laser unit to horizontally align
the laser beam and dichroic mirror centering tool
opening (for the EX).

Figure 11.3-9

5. Repeat Steps 3 and 4 until the laser beam
passes through the center of the dichroic mirror
centering tool opening (for the EX). The
centering tolerances should be within
approximately 0.2 mm. Centering can be easily
checked by holding a sheet of white paper
behind the tool opening.
6. Turn the light control knob to the MAX position
on the continuous light control area and confirm
that the laser beam is not clipped.
After checking, return the knob to the MAX
position on the step-by-step light control area to
adjust the laser.

Figure 11.3-10
Adjustment hole
Ar mirror unit
Clamp screw
149

7. Tighten the Ar mirror clamp screw and the four mounting plate screws to secure the Ar laser unit in
place.
8. Close the shutter of the laser unit.

3-2 Adjusting the HeNe laser and 405 cylindrical laser

1. Install the dichroic mirror centering tool (for the EX) on the dichroic mirror unit.
2. Open the shutters for the HeNe laser and 405 cylindrical laser (set to the OPEN position). Then
open the shutter in the HeNe laser optical path on the laser unit side. At this time, you may insert a
hex wrench into the shutter to keep the shutter open.

3. Adjust the 405 cylindrical laser orientation so
that the beam diameter of the 405 cylindrical
laser forms an upright ellipse.

Figure 11.3-11

4. Adjust by moving the rear mount with the front
mount fixed so that the laser beam passes
through the center of the dichroic mirror
centering tool opening (for the EX). If this cannot
be accomplished by moving just the rear mount,
the front mount may also be moved.
5. Turn the light control knob to the MAX position
on the continuous light control area and confirm
that the laser beam is not clipped.
After checking, return the knob to the MAX
position on the step-by-step light control area to
adjust the laser.

Figure 11.3-12

6. Tighten the two sleeve-retaining screws below the center, leaving the sleeve-retaining screw above
the center and the slot-tightening screw loose. This permits later adjustment of laser polarization
that requires rotation of the entire laser unit. (No polarization adjustment is required for the 405
cylindrical laser.)
The centering tolerances should be within approximately 0.2 mm.
7. Adjust the other laser in the same way.
8. Close the shutter of the laser unit side.
When the AOM is not used, this completes the rough adjustment of the lasers. Proceed to Section
11.4, Laser Rough Adjustment 2.

150
4

Attaching the AOM [only when the AOM unit is used]

1. Turn off power to all units.
2. Remove the tape covering the openings on the AOM.

3. Detach the two light control units at which the
AOMs will be attached, and attach the two
AOMs using three screws provided.
Standard positions for attaching the AOMs are
those of the Ar laser unit and the G-HeNe laser
unit.

Figure 11.3-13

4. Detach the metal plates of the laser unit and
attach the AOM cable connectors with panels.

Figure 11.3-14

5. Connect the AOM cables to the AOMs, routing
AOM1 to the Ar laser unit and AOM2 to the
G-HeNe laser unit.
6. Wire the cables as shown in the photograph and
secure with cable retainers.

Figure 11.3-15

AOM cable connector
151
5

Adjusting the AOM [only when the AOM unit is used]

1. Loosen the X-axis clamp screws, Z-axis clamp
screw and rotation clamp screws of the AOM.
2. Open the laser shutter, turn off the power for the
AOM controller, and then adjust along the X- and
Z-axes so that the laser beam passes through
the AOM opening.
To adjust in the Z-axis direction, use the
adjusting screw with the clamp screw completely
loosened.
The transmission ratio of after-AOM to pre-AOM
should be approximately 85%.

Figure 11.3-16

Figure 11.3-17

3. Detach the laser unit front sheet metal cover.

4. Confirm the AOM controller power switch is
turned off, and then remove the AOM controller
cover.

Figure 11.3-18
Rotation
adjuster screw
X-axis
clamp screw
Z-axis adjuster screw
(setscrew)
Rotation clamp screw
Z-axis
clamp screw
X-axis
clamp screw

Driver for
AOM1
Driver for
AOM2
152

5. Insert the flat-blade screwdriver into the BIAS
volume (BIAS) and turn it all the way clockwise.

Figure 11.3-19

6. Start the C2 system including the AOM controller.

7. Set the AOM control to the maximum output.
Start the operation tool software C2Tool; click
the [Control] toggle button and select the [Laser
Power] tab.
Uncheck the [Calibration] check box (not
selected).
Select % in the [Input] combo box.
Set [Power] of the [Lasers] group box to 100.0
(%).
Check the [Shutter] checkbox (selected) of the
[Lasers] group box to open the shutter of the
laser to be set.

Figure 11.3-20

Driver for
AOM1
Driver for
AOM2
153
8. Temporarily set the PWR volume (PWRADJ) of
the AOM driver.

WARNNIG

The AOM controller is connected to the primary
power supply. Be careful to avoid touching the
primary side.

When the AOM controller is turned on, laser light
passing through the AOM is split into multiple
beams. The light transmitted when the AOM
controller is off is called the zeroth-order light.
When the power is turned on, the 1st-order light,
the 2nd-order light, and the third-order light
appear on both sides of the zeroth-order light.
The 1st-order light is used for this laser unit;
however, the 1st-order light to be used differs
depending on how the AOM is placed. See Figure
11.3-21.
Loosen the rotation clamp screws, and roughly
adjust the rotation direction of AOM so that the
1st-order light will be approximately maximum.
About the 1st-order light, the one on the left side
facing the laser emitter end is used for the L2
laser and the other one on the right side facing
the laser emitter end is used for the L3 laser.
To measure the laser power, place a power
meter about 2 m from the AOM so that it is
struck only by the 1st-order light.
Temporarily set the PWR volume (PWRADJ) of
the AOM driver so that it will be 40 to 50% of the
maximum output. (The maximum output here
means the maximum output that is obtained
when the PWR volume is rotated.)
If the PWR volume (PWRADJ) is overrotated in
clockwise direction, an excess current may pass
through the AOM components resulting in AOM
failure. Be careful not to overrotate the PWR
volume in clockwise direction.
Use the 1st-order light for all subsequent
adjustments.

Figure 11.3-21
Z
e
r
o
t
h
-
o
r
d
e
r

l
i
g
h
t
1
s
t
-
o
r
d
e
r

l
i
g
h
t
Use this 1st-
order light.
2
n
d
-
o
r
d
e
r

l
i
g
h
t
2
n
d
-
o
r
d
e
r

l
i
g
h
t
1
s
t
-
o
r
d
e
r

l
i
g
h
t

L3 laser beam L2 laser beam
Z
e
r
o
t
h
-
o
r
d
e
r

l
i
g
h
t
1
s
t
-
o
r
d
e
r

l
i
g
h
t
Use this 1st-
order light.
2
n
d
-
o
r
d
e
r

l
i
g
h
t
2
n
d
-
o
r
d
e
r

l
i
g
h
t
1
s
t
-
o
r
d
e
r

l
i
g
h
t

154

9. Adjust the AOM rotation direction.
In this state, make the final adjustment of the rotation direction of AOM so that the 1st-order light
will be maximum.
The brightness may increase slightly when the beam opening of the AOM is finely adjusted in the X
and Z directions.

10. Carefully tighten the X-axis clamp screws, Z-axis
clamp screw and rotation clamp screws of the
AOM.

Figure 11.3-22

6

Adjusting the AOM driver [only when the AOM unit is used]

After optical axis adjustment of AOM, adjust the AOM driver.

1. Set the AOM control to the minimum output.
Click the [Control] toggle button and select the
[Laser Power] tab.
Set [Power] of the [Lasers] group box to 0.0 (%)
and check the [Shutter] checkbox (selected) to
open the shutter of the laser to be set.

Figure 11.3-23

2. Adjust the BIAS volume (BIAS) of the AOM
driver.
Measure the laser intensity of the 1st-order light
with the power meter, and adjust the BIAS
volume (BIAS) of the AOM driver so that the
laser intensity will be minimum.

Figure 11.3-24

3. Set the AOM control to the maximum output
again.
Click the [Control] toggle button and select the
[Laser Power] tab.
Set [Power] of the [Lasers] group box to 100.0
(%) and check the [Shutter] checkbox (selected)
to open the shutter of the laser to be set.

Figure 11.3-25
155

4. Adjust the AOM driver's PWR volume
(PWRADJ).
Measure the laser intensity of the 1st-order light
with the power meter, and adjust the PWR
volume (PWRADJ) of the AOM driver so that the
laser intensity will be maximum. Be careful not to
overrotate the PWR volume in clockwise
direction.
Although the guideline is 70% or more of the
zeroth-light, it may not reach 70% due to the
beam diameter of laser.
By turning off the power of the AOM controller,
the location of the zeroth-order light can be
confirmed, and its power can be measured.
Turn the PWR volume (PWRADJ)
counter-clockwise from the position of the
maximum brightness.
For Ar and G-HeNe lasers, reduce to
approximately 98% of the maximum brightness.
For R-HeNe laser, reduce to approximately 80%
of the maximum brightness.
PWR adjustment should be finalized where the
PWR volume is turned in the clockwise direction.
To make fine adjustments, briefly rotate the
PWR volume counter-clockwise, then slowly turn
it back in the clockwise direction.
This adjustment must be performed to prevent
too much power from being applied to the AOM.
Stop at the limit position if the brightness does
not fall to 98% when the PWR volume is turned
fully counter-clockwise for the Ar laser.

Figure 11.3-26

7

Attaching the AOM controller cover

Attach the cover to the AOM controller.

156
1

Attaching the dichroic mirror adjustment unit

1. Attach the three dichroic mirror adjustment units,
aligning the pin positions and partially tightening
two screws on each unit.
The following color mirrors are attached for each
laser:
L1 (405, 633): Violet
L2 (488): Blue
L3 (543): Total reflection

Figure 11.4-1

2. Attach the spring while pressing it against the
side of each dichroic mirror adjustment unit.

Figure 11.4-2

3. Rotate the unit to verify that the bottom surface of the dichroic mirror adjustment unit is making a
secure contact. The unit should rotate smoothly.

Press
157
2

Adjusting the angle of Ar dichroic mirror adjustment unit

(The dichroic mirror adjustment unit adjusts the laser angle.)

1. Place the dichroic mirror centering tool (for the
EX) in front of the fiber coupling.

Figure 11.4-3

2. Loosen the two horizontal rotation clamp screws
of the Ar dichroic mirror adjustment unit.

Figure 11.4-4

3. Adjust the horizontal axis setscrews and align
the horizontal axis of the laser light with the tool
opening.
If the AOMs are attached, first turn off the AOM
controller and check the zeroth-order light
position. Always check that the 1st-order
(stronger) light is aligned with the hole in the
tool.
Check that the 1st-order light is used for
subsequent adjustments.

Figure 11.4-5

4. Loosen the vertical clamp screw of the Ar
dichroic mirror adjustment unit.
The L1 and L3 clamp screws are spring
plungers.
Rotate 90 degrees back from the tightened
position in order to apply tensions to the springs.

Figure 11.4-6
158

5. Adjust the vertical axis setscrew and align the
vertical axis of the laser light with the tool
opening.

Figure 11.4-7

6. Carefully tighten the horizontal clamp screws.

Figure 11.4-8

7. If the beam becomes misaligned from the center after tightening, fine-adjust the vertical axis
setscrew to align the beam with the tool opening.
8. Carefully tighten the vertical clamp screw.

This completes the adjustment of the Ar dichroic mirror adjustment unit.

159
3

Removing the front sheet metal cover

Remove the three screws and remove the front sheet
metal cover.

Figure 11.4-9

4

Coarse-adjusting the angle of the other two dichroic mirror adjustment units

1. As a target, prepare a piece of paper on which
crosshairs are drawn.
Place the target at least 50 cm from the fiber
attachment unit, aim the Ar laser, and clamp it at
the position at which the beam strikes the center
of the target.

Figure 11.4-10

2. Using the procedure shown in 2 Adjusting the angle of Ar dichroic mirror adjustment unit, adjust
the dichroic mirror adjustment units vertically and horizontally so that the beams from the other two
lasers also roughly coincide with the target.

160
5

Adjust the laser polarization orientations (except for the 405 laser)

1. Place the polarizer-equipped tool in front of the
fiber coupling with the index facing forward.
2. Open the Ar laser shutter to emit the Ar laser
only.
3. Place a piece of white paper behind the fiber
coupling and move the polarizer knob to provide
minimum light transmission.

Figure 11.4-11

4. Open the other shutters and emit the other two
lasers.
5. Rotate the laser heads for those two lasers to
find the position that gives the darkest results.
Do not alter the polarizer orientation from the
position determined for the Ar laser.

Figure 11.4-12

6. Tighten the slot-tightening screws (1 each) on
the two other lasers after adjusting the polarizer
orientation, and then tighten one
sleeve-clamping screw above the center.

Figure 11.4-13

Figure 11.4-14

7. Remove the polarizer-equipped tool.

161
6

Fine-adjusting the angle of the other two dichroic mirror adjustment units and
adjusting the beam shift unit

1. Adjust the angle of the dichroic mirror
adjustment unit and align the HeNe laser beam
position with the target positioned at a distance
of 50 cm.
Since the beam diameter of the 405 cylindrical
laser is upright elliptical, estimate a center point
and use this for alignment.

Figure 11.4-15

2. Adjust the beam shift unit to align the positions
of the other laser beams with the position of the
Ar laser beam scattered by the mirror reflector in
front of the fiber coupling.
Adjust horizontally either by rotating the beam
shift unit manually or by inserting a hex wrench
in the side and turning the horizontal axis
setscrew.
Adjust vertically by moving the vertical axis
setscrew up or down with the vertical axis clamp
screw loosened.

Figure 11.4-16

Figure 11.4-17
Mirror reflector
Horizontal axis setscrew
162

Figure 11.4-18

3. Repeat Steps 1 and 2 to make the light from the three lasers coincide with the Ar laser beam
position on the mirror reflecting surface and the target position at a distance of 50 cm.
(Take enough time to perform this step thoroughly. Getting the laser beams to coincide here will
make it easier to adjust transmission into the optical fiber.)

Vertical axis clamp screw
(nearer to the axis A)
Vertical axis setscrew
(farther from the axis A)
Axis A
11 Setting up 3 Laser Unit EX >> 11.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber
163
11.5 Final Adjustment for Transmitting Ar Laser Light through
Single-mode Optical Fiber
1

Centering the Ar laser

Align the Ar laser beam with the coupler centering tool pinhole.

1. Loosen the rotation clamp screw on the coupler
unit.

Figure 11.5-1

2. Set the coupler centering tool pinhole to the
laser incident side and place a piece of paper on
the exit side, and adjust with the laser incident
side adjustment screws H (horizontal) and V
(vertical) to find the point giving maximum
intensity.

Figure 11.5-2

3. Set the coupler centering tool pinhole to the
laser exit side and adjust with the fiber side
adjustment screws H (horizontal) and V (vertical)
to find the point giving maximum intensity.

Figure 11.5-3

4. Repeat Steps 2 and 3 several times to find the point of maximum brightness.

Laser incident side
adjustment screw
Fiber side adjustment
screw
Rotation clamp
screw (center)
164
2

Attaching the fiber coupling

1. Close all laser shutters.

2. Tighten the three screws and attach the fiber
coupling unit. Since this will be rotated at a
subsequent step for adjusting the polarizing
orientation, do not tighten excessively at this
time. Tighten so that the units can still be rotated
by inserting the wrench in the tool opening.
Make sure that the key groove of the fiber
coupling faces upward.
Handle the coupling carefully to keep out debris.
Figure 11.5-4

3

Transmitting Ar laser light through the fiber

WARNING

Never look directly at the fiber tip. Always monitor laser emissions by aiming the tip down onto
a sheet of paper.

1. Carefully align the end of the optical fiber (the
end without the tag) with the key groove of the
coupling and insert it as far as it will go. Carefully
tighten the optical fiber connector. Gradually
tighten and stop when light is first emitted from
the tip of the fiber.

Figure 11.5-5

2. If no light is emitted from the end of the fiber in
Step 1, loosen the fiber connector approximately
10 degrees, gradually pull out the fiber, and
observe whether light is emitted. (Light is emitted
more easily if defocused.) If this is not effective,
repeat loosening the connector and pulling the
fiber a couple of times.

Figure 11.5-6
165

3. If even a small amount of laser light is observed,
adjust the two fiber side adjustment screws of
the coupling to find the point at which the Ar
laser light increases in intensity. (Final fine
adjustment will be performed after adjusting the
polarizing orientation.)
4. Gradually tighten the fiber connector. Stop just
before the point at which light is no longer
emitted. Now adjust the two fiber side
adjustment screws to find the point at which the
laser light increases in intensity. Repeat this
procedure to ensure a maximum intensity,
checking to confirm that the fiber connector is
tightened.
5. If no light is emitted even after repeating these
steps, repeat the procedure from 1 Centering
the Ar laser in Section 11.5, Final Adjustment
for Transmitting Ar Laser Light through
Single-mode Optical Fiber.
6. Tighten the fiber connector at the point giving a
maximum Ar laser light brightness. Check that
the fiber has no laxity.
7. Temporarily close the shutter for the Ar laser.

Figure 11.5-7

4

Adjusting the fiber polarizing orientation

1. Align the knob on the polarizer-equipped tool
with the index. Mount the polarizer-equipped tool
on the polarizing orientation adjustment tool with
the knob at the top.

Figure 11.5-8

2. Clamp the emission end of the optical fiber to the
polarizing orientation adjustment tool. Place a
light-scattering target (a piece of paper, etc.)
before it.
Position the fiber so that it is not subjected to
excessive force, and so that both ends are laid
out straight.

Figure 11.5-9
166

3. Open the Ar laser shutter. Insert a tool into the
coupling and rotate while monitoring the laser
light image on the target to find the point giving
the minimum brightness. (If three screws on the
coupling are too tight, loosen them slightly
before rotating.)

Figure 11.5-10

4. Place the power meter in front of the polarizer to
measure the laser power.

5. Rotate the polarizer-equipped tool 90 degrees so
that the knob faces to the side, place the power
meter in front of the polarizer, and measure laser
power.
6. Check that the extinction ratio (ratio of bright to
dark laser power) is at least 20:1.
If not, repeat the adjustment procedure from
Step 1.

Figure 11.5-11

7. Tighten the three clamping screws on the coupling.

5

Adjusting to give maximum Ar laser light transmission through the fiber

1. Attach the emitter end of the optical fiber to the
power meter tool and adjust to find the point
giving maximum intensity, while measuring the
intensity on the power meter. To adjust intensity,
use either the laser incident side adjustment
screw H or adjustment screw V, together with
the fiber side adjustment screws V and H.
As a guide to Ar laser incidence efficiency, the
brightness at the end of the coupler should be at
least 50% of the brightness in front of the
coupler.
(Transmitting the light through the AOM will
distort the beam shape and result in a lower
transmission ratio than without the AOM.)

Figure 11.5-12
Setscrew at the coupling
Fiber side
adjustment screw
Laser incident side
adjustment screw
167

(Adjustment example)
(1) Turn the laser incident side adjustment
screw H 30 to 60 degrees in one direction.
(2) Turn the fiber side adjustment screw H in
the same direction as (1) to find the point
giving a maximum intensity. If brightness
does not increase, turn the laser incident
side adjustment screw H from 30 to 60
degrees in the direction opposite to (1).
Turn the fiber side adjustment screw H in
the same direction to find the point giving a
maximum intensity.
(3) Repeat the adjustment in (2) to find the
point in the horizontal axis giving a
maximum brightness.
(4) Turn the laser incident side adjustment
screw V 30 to 60 degrees. Turn the fiber
side adjustment screw V in the same
direction to find the point giving a
maximum intensity.
(5) Repeat the adjustment in (4) to find the
point in the vertical axis giving a maximum
brightness.
(6) Repeated adjustments along the horizontal
and vertical axes may result in even
greater brightness.

Figure 11.5-13

Figure 11.5-14

2. Tighten the two clamp screws on the coupler,
and then tighten the rotation clamp screw.

Figure 11.5-15

3. Record the Ar laser brightness. (Note the power meter wavelength setting.)
4. Open the other laser shutters and check that the laser light is emitted. If no light is emitted from the
other lasers, repeat the adjustments in Sub section 3-2, Adjusting the HeNe laser and 405
cylindrical laser in Section 11.3, Laser Rough Adjustment 1.

11 Setting up 3 Laser Unit EX >> 11.6 Final Adjustment for Transmitting HeNe Laser and 405-nm Laser Light through Single-mode Optical Fiber
168
11.6 Final Adjustment for Transmitting HeNe Laser and 405-nm
Laser Light through Single-mode Optical Fiber
The procedure given below describes how to transmit laser light through the fiber by adjusting the dichroic mirror
adjustment unit and beam shift unit alone, without using the coupler.

1. Open the shutter only for the laser to be used.
Close the shutters for the other lasers.

2. Adjust the horizontal and vertical axis setscrews
on the dichroic mirror adjustment unit while
monitoring the brightness on the power meter to
find the point giving a maximum brightness.
To reduce tightening deviations, tighten the two
horizontal axis clamp screws to the point at
which the dichroic mirror is able to rotate before
adjusting horizontal rotation.

Figure 11.6-1

3. Adjust the beam shift unit to find the point giving
a maximum brightness.
Adjust horizontally either by rotating the beam
shift unit by hand or by inserting a hex wrench in
the tool hole.
Adjust vertically by moving the vertical axis
setscrew up or down with the clamp screw
loosened. Approximately 45% should be
achieved in front of and behind the fiber coupling
for each laser.
(Transmitting the light through the AOM will
distort the beam shape and result in a lower
transmission ratio than without the AOM.)
For the 405 cylindrical laser, this figure is
approximately 10%.

Figure 11.6-2

Figure 11.6-3

Tool hole
Vertical axis
setscrew
Vertical axis
clamp screw
Horizontal axis
clamp screw
11 Setting up 3 Laser Unit EX >> 11.6 Final Adjustment for Transmitting HeNe Laser and 405-nm Laser Light through Single-mode Optical Fiber
169

4. Carefully tighten the beam shift units V-axis
clamp screw. Readjust the rotation axis on the
beam shift unit if the brightness decreases here.
Then carefully tighten the H-axis clamp screw.

Figure 11.6-4

Figure 11.6-5

5. Once the point giving a maximum brightness is
found, carefully tighten two H-axis clamp screws
alternately on the dichroic mirror adjustment unit.
If the brightness decreases after tightening,
readjust the vertical axis.
6. Carefully tighten the vertical axis clamp screw.
Excessive tightening is not necessary if large
tightening deviations occur.
7. Record the brightness for each laser.
8. Close all the laser shutters.

Figure 11.6-6

Figure 11.6-7

Horizontal axis clamp screw
11 Setting up 3 Laser Unit EX >> 11.7 Sheet Metal Cover Attachment
170

1. Attach four harness bands to the laser unit
mount.

Figure 11.7-1

2. Secure the cables and fibers with these harness
bands.

Figure 11.7-2

3. Attach the sheet metal cover using six screws.

4. Align the white line on the knob with the control
range indicator for the light control knob.

Figure 11.7-3
Harness band retainer
11 Setting up 3 Laser Unit EX >> 11.7 Sheet Metal Cover Attachment
171

5. Affix the label provided corresponding to the
laser wavelength used.

Figure 11.7-4

This completes the laser adjustment.

172

WARNING

The unit must be set up as instructed by the local LSO (Laser Safety Officer).
This laser unit uses Class IIIb lasers. Accidental exposure of eyes or skin to laser light may
result in injury or other problems. Do not turn on the lasers until they are installed in the laser
unit.
Always wear laser-protection goggles while lasers are turned on.
Do not place tools or optical components such as mirrors in the light path while lasers are
turned on.
Use the lowest output power rating during setup. For Ar lasers, use STANDBY mode during
setup.
The laser units are heavy. Without the lasers installed, the 4 laser unit A weighs approximately
43 kg. Do not attempt to lift the laser unit unassisted.
The laser manual must be provided to the user.

CAUTION

To use a laser diode or a solid-state laser with the spectral detector unit, its wavelength must be
measured with a spectrometer and registered into the C2SetupTool beforehand.

The setup procedure for the 4 laser unit A is given below.
12.1 Preparation
12.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber
12.6 Final Adjustment for Transmitting Other Laser Light through Single-mode Optical Fiber
12.7 AOTF Driver Remote Controller Detachment
12.8 Operation Check

12 Setting up 4 Laser Unit A
12 Setting up 4 Laser Unit A >> 12.1 Preparation
173
12.1 Preparation

1


Confirm that the following accessories supplied with
the LU4-C2 connection kit (MHF41412) or 4 laser unit
A (MHF41402) have been provided:

< LU4-C2 connection kit >
Cable (x 3)
4 laser unit cable
PCI6713 cable
Interlock cable
Shorting pin jack
Rectangular shorting connector
Caution label

< 4 laser unit A >
561-nm laser adapter
561-nm laser adapter cover
561-nm laser setscrew (x 4)
Black hexagonal bolt (x 4)
Black ring (x 4)
Harness band (x 6)
Laser wavelength label

Figure 12.1-1

Figure 12.1-2

2

Detaching the fixing blocks

1. Unscrew the screws on both side plates of the 4
laser unit A. (There are 12 screws to be
unscrewed. Six on the right side and six on the
left side.) Then, detach two side plates.
2. Loosen the screws on the sheet metal cover on
the front part and detach the sheet metal cover.

Figure 12.1-3
Sheet metal
cover
Side plate
174

3. Unscrew each hexagonal bolt on the fixing block
at four locations, and then remove the four fixing
blocks.

Figure 12.1-4

4. Take a hexagonal bolt of Step 3. Insert it into two
fixing blocks. Fix them on the basement inside
the body near the right side plate. Fix the other
two hexagonal bolts on the basement inside the
body near the left side plate.

Figure 12.1-5

Figure 12.1-6 Right side basement

Figure 12.1-7 Left side basement
Four locations

Hexagonal
bolt

Fixing
block
Bolt
175

5. Slide four covers to close the holes of the
hexagonal bolts of the fixing blocks and fix the
covers.

Figure 12.1-8

3

Setting up the PC and D/A board

1. Set up the PC and D/A board (PCI-6711, PCI-6713, or PCI-6733, provided by the distributer) to
control AOTF. Perform Section 14.1, D/A Board Installation [Only When AOM Unit or 4 Laser Unit
A Is Used] in Chapter 14, Setting up PC and Software.

4

Wiring

1. Connect the CONTROLLER connector on the 4
laser unit A and the 4LU connector on the C2
controller using the 4 laser unit cable supplied
with the LU4-C2 connection kit.
2. Connect the CONF connector on the 4 laser unit
A and the ILK connector on the C2 controller
using the Interlock cable supplied with the
LU4-C2 connection kit.
3. Connect the PC/AOTF connector on the 4 laser
unit A and the connector on the D/A board of the
PC using the PCI-6713 cable supplied with the
LU4-C2 connection kit.
4. Connect the shorting pin jack and rectangular
shorting connector supplied with the LU4-C2
connection kit to the TIRF connector and LUSU
connector on the 4 laser unit A.

Figure 12.1-9

LUSU
TIRF PC/AOTF
CONTROLLER
176
5

Attaching the black ring (accessory)

1. Remove the bolts and nuts at four locations of
the laser inlets. Be careful not to drop the bolts
and nuts. (Hold the bolts and nuts with fingers to
prevent them from falling.)

Figure 12.1-10

Figure 12.1-11

2. Affix the black rings (sponges) to the openings.
The center of the ring must be aligned with the
center of the opening.

Figure 12.1-12

6

Removing the fiber coupling

Loosen three knurled screws on the outer side of the
optical fiber coupling device and remove the optical
fiber coupling device. Handle the coupling carefully to
prevent contamination of the space inside.

Figure 12.1-13

Four locations
Black ring
12 Setting up 4 Laser Unit A >> 12.2 Laser Installation
177
Table 12.2-1 shows the recommended lasers to be mounted on the 4 laser unit A.

Figure 12.2-1


Table 12.2-1
L1 L2 L3 L4
4 laser unit A 638-nm laser
405-nm laser
440-nm laser
Ar laser (single line or
multiple lines)
G-HeNe laser

178
1

Installing the Ar laser

1. Unscrew the four hexagonal bolts on the 488-nm
laser adapter attached to the 4 laser unit A body.
And then, detach the 488-nm laser adapter from
the 4 laser unit body.
2. Attach the laser adapter detached in Step 1 onto
the Ar laser.

Figure 12.2-2

Precaution:
Note that the screw positions of the laser
adapter for the multiple lines Ar laser (black
body) differ from that for the single line Ar
laser (white body).
For the multiple lines Ar laser:
Three near-side holes
For the single line Ar laser:
Three far-side holes

3. Mount the Ar laser to the L3 position on the 4
laser unit A. The laser emission port must
contact the black ring to prevent scattered light
of laser from leaking. Press the laser emission
port against the black ring. The black ring
becomes flattened. Fix the laser body
temporarily with four hexagonal bolts of Step 1.

Figure 12.2-3

Figure 12.2-4

488-nm laser adapter
Ar laser side
(near side)
Screw holes for
the single line Ar laser
Screw holes for the
multiple lines Ar laser
Black ring Attach while pressing
against the black ring just
enough to compress it.
Four locations
179
2

Installing the 405 (440)-nm laser and 638-nm laser

1. Remove six hexagonal bolts (three bolts each) at
the L2 position and L1 position of the 4 laser unit
A for the 405-nm (440-nm) laser and 638-nm
laser.

2. Mount the 405 (440)-nm laser to the L2 position
of the 4 laser unit A. And then mount the 638-nm
laser to the L1 position on the laser unit. The
laser emission port must contact the black ring to
prevent scattered light of laser from leaking.
Press the laser emission port against the black
ring. The black ring becomes flattened. Fix the
laser body temporarily with four hexagonal bolts
of Step 1.

Figure 12.2-5

3. Do not connect the LD modulation cable. If
connected, the device does not operate
correctly.

(Connectors on the 4 laser unit A rear panel)
Figure 12.2-6

CAUTION

Put on laser-protection goggles in 405-nm laser adjustment.
(E.g.: Yamamoto Kogaku 1/100-attenuation Blue Laser Diode YL331M)

Press against the black rings
Fix with hexagonal bolts
Do not connect a cable to
these LD connectors.
180
3

Installing the G-HeNe laser

Mount the laser so that the polarization orientation of the laser light is vertical by using the
polarizer-equipped tool provided with the G-HeNe laser.
When the polarization orientation of the laser light is vertical at the incident port of the AOTF, the
polarization orientation of the laser light is horizontal at the emission port of the AOTF.

1. Align the protrusion of the polarizer-equipped
tool to the mark.
Figure 12.2-7

2. Mount the polarizer-equipped tool to the place.
The curved side of the polarizer-equipped tool
faces the front of the 4 laser unit A as shown in
the figure at right.

Figure 12.2-8

Laser unit front
Protrusion Mark
181

3. Loosen two hexagonal bolts at the slots of the
G-HeNe laser adapters. And then, insert the
G-HeNe laser into the sleeve of the laser
adapters.
4. The laser emission port must contact the black
ring to prevent scattered light of laser from
leaking. Press the laser emission port against
the black ring. The ring becomes flattened.
Confirm that the label on the HeNe laser faces
upward when installed.

Figure 12.2-9

5. Put a target such as a piece of paper in the
optical path away from the polarizer-equipped
tool.
6. Manually open the L4 laser shutter to emit the
laser light. At this time, insert a hex wrench into
the shutter to keep the shutter open.

Figure 12.2-10

7. Rotate the G-HeNe laser body to adjust the laser
light on the target. The rotation of the laser body
must be stopped at the position where the laser
light is the darkest.
8. Tighten two hexagonal bolts at the slots of the
G-HeNe laser adapters to fix the G-HeNe laser.
Figure 12.2-11

Label Slot x 2
Press against the black ring
182
4

Installing the 561-nm solid-state laser

Normally, the G-HeNe laser adapters are attached to the 4 laser unit A at the product is shipped from the
factory. To use the 561-nm solid-state laser, loosen four hexagonal bolts of the G-HeNe laser adapters
and remove the G-HeNe laser adapters along with the hexagonal bolts.

1. Attach the 561-nm laser adapter provided with
the 4 laser unit A onto the 561-nm laser. And
then, attach a black ring to the laser emission
port.

Figure 12.2-12

2. Mount the 561-nm laser body onto the L4
position. Fix the laser body with four 561-nm
laser setscrews provided with the 4 laser unit A.

3. Detach the screw near the emission port of the
561-nm laser adapter temporarily.

Figure 12.2-13

4. Attach the 561-nm laser adapter cover provided
with the 4 laser unit A and fix it with the screw
detached in Step 3.

Figure 12.2-14

This is the end of the laser installation.

561-nm laser
561-nm laser adapter Black ring
Laser adapter cover
183
5

Laser warm-up and AOTF warm-up

1. Remove transparent tape at the inlet and outlet
of the AOTF unit.

Figure 12.2-15

2. Warm up the lasers and the AOTF
(1) Turn off the REMOTE switch on the back
of the laser unit.
(2) Turn on the POWER switch on the back of
the laser unit and the lasers. Allow the unit
to warm up for 20 minutes or longer.
When the REMOTE switch is turned off,
the 4 laser unit A can be turned on or off
independently.
When the REMOTE switch is turned on,
the power control of the 4 laser unit A is
linked with the controller.
* After the adjustment of the 4 laser unit
A, turn on the REMOTE switch.

Figure 12.2-16

Precautions when warming up the laser unit
Leave the lasers turned on for approximately 20 minutes before adjusting. Making adjustments
immediately after turning on the lasers may result in shifts from adjusted levels.

Transparent tape
REMOTE switch
12 Setting up 4 Laser Unit A >> 12.3 Laser Rough Adjustment 1
184
1

Checking the initial beam shift unit position

1. The tool hole and the horizontal clamp screw on
the side of the beam shift part must be aligned
vertically.
2. The cylindrical glass must not tilt significantly.
* Check all beam shift parts in Steps 1 and 2. (By
default, the beam shift parts are arranged as shown
in the figure at right.)

Figure 12.3-1

2

Connecting the AOTF driver remote controller

Connect the cable of the AOTF driver remote
controller to the USB connector of the AOTF driver at
the lower part of the laser unit.

Figure 12.3-2

AOTF driver remote controller

Connect the AOTF remote controller cable to the USB port of the AOTF driver. When connected,
the remote controller screen displays External mode. To change the screen on the controller to
Internal mode, press any numeric key on the controller. Then, press the numeric key assigned to
the required laser wavelength. (See Table 12.3-1 for the key assignment list.) The wavelength
number will be displayed on the screen and the corresponding laser beam starts irradiating. To
cancel the selection, press the key again.
The frequency and the power level can be set for individual laser wavelength.
AOTF driver USB
connector

Tool hole
Horizontal axis
clamp screw
Cylindrical glass
185

Table 12.3-1 Laser wavelengths available with the AOTF driver remote controller
On the screen Wavelength Laser
L1 457 nm
Ar laser
L2 477 nm
L3 488 nm
L4 514 nm
L5 543 nm G-HeNe laser
L6 561/594 nm 561/594-nm solid-state laser
L7 405/440 nm 405/440-nm laser
L8 638 nm 638-nm laser

3

Adjusting the Ar laser position

In the 4 Laser unit A, the coupled laser light does not
pass through the AOTF before the laser coupling
mirror part. The AOTF is located beyond the laser
coupling mirror part where the four laser lights are
coupled.
1. Select the line three (L3) with the AOTF remote
controller to use the 488-nm Ar laser.
Figure 12.3-3

2. Unscrew the two hexagonal bolts at the laser
coupling mirror part and detach the laser
coupling mirror part.

Figure 12.3-4

Precautions:
At the detachment of the laser coupling mirror
part, the collimation pin may come with the
laser coupling mirror part. Be careful to
prevent the pin from getting lost.

3. If the collimation pin comes with the laser
coupling mirror part, take the collimation pin from
the laser coupling mirror part and restore the pin
into the hole again. Attach the centering tool to
the collimation pin. Rotate the centering tool so
that the laser light enters the hole of the
centering tool at a right angle.

Figure 12.3-5
Hexagonal bolts
Centering tool
186

4. Put a target such as a piece of paper in the
optical path away from the centering tool.

Figure 12.3-6

5. Open the shutter of the Ar laser (the OPEN
position). And then, manually open the L3 laser
shutter.

Figure 12.3-7

6. The laser light direction can be adjusted
horizontally by moving the laser body with
hands.

Figure 12.3-8

7. Attach the centering tool to the laser unit at
position A shown in the figure at right. Adjust the
laser light direction horizontally by moving the
laser body or vertically by moving the Ar laser
mirror adjustment part so that the laser light
enters the Hole L (see the figure shown at right).

Figure 12.3-9

A
Ar laser shutter
187

8. Attach another centering tool to the laser unit at
Position B shown in the figure at right. Adjust the
beam shift part with respect to the centering tool
at Position A and move the laser body with
respect to the centering tool at Position B so that
the Ar laser light roughly enters the Hole L of
each centering tool.
9. Tighten the clamp screws of both the Ar laser
body fixing part and the Ar laser mirror
adjustment part to secure the respective parts.

Figure 12.3-10

Figure 12.3-11

10. Fully loosen the clamp screw at the right of the
Ar laser mirror adjustment part viewed from the
laser coupling mirror part to the laser body. And
loosen the clamp screw to the halfway point at
the left of the Ar laser mirror adjustment part.

Figure 12.3-12

11. Insert a screw driver into the adjustment hole on
the Ar laser mirror adjustment part. And pry it to
adjust the laser light position in the vertical
direction. Aim the laser light at the hole of the
centering tool attached in Step 3 by moving the
screwdriver.

Figure 12.3-13
Position A Position B
Zeroth-order light
1st-order light

Tighten temporarily
Tighten securely
Ar mirror adjustment part
Adjustment hole
188

12. Attach another centering tool onto the collimation
pin on the left side viewed from the front of the 4
laser unit A. Rotate the centering tool so that the
laser light enters the hole of the centering tool at
a right angle.
(The centering tool attached in Step 3 must be
untouched. Two centering tools are used on the
laser unit to adjust the laser position.)

Figure 12.3-14

Figure 12.3-15

13. Loosen the clamp screw for the horizontal
direction of the L3 beam shift part.
horizontally by rotating the whole L3 beam shift
part by hands. Aim the laser light at the hole of
the centering tool attached in Step 12 by rotating
the L3 beam shift part.
15. Loosen the clamp screw for the vertical direction
of the L3 beam shift part.
vertically by rotating the setscrew for the vertical
direction of the L3 beam shift part. Aim the laser
light at the hole of the centering tool of Step 12
by rotating the setscrew for the vertical direction.
17. Repeat steps 6, 11, 14, and 16. The laser light
must pass through the holes of the centering
tools.

Figure 12.3-16
Centering tool
attached in Step 3
Centering tool
attached in Step 12
Centering
tool
Rotate by
hand
Vertical clamp screw
Vertical setscrew
Axis A Horizontal
clamp screw
189

18. After the completion of the adjustment, tighten
two clamp screws of the Ar laser mirror
adjustment part to fix the Ar laser mirror
adjustment part. And then, fix the Ar laser body
with four hexagonal bolts.

Figure 12.3-17

Figure 12.3-18

19. Check that the zeroth-order light from the AOTF
unit is shielded by the shield plate. And then,
close the shutter of the optical path for the Ar
laser light.
If the zeroth-order light is not shielded, the
position of the shield plate must be adjusted.
Loosen the clamp screw of the shield plate, and
then adjust the position so that the shield plate
stops the zeroth-order light.

Figure 12.3-19

20. Remove the centering tool attached in Step 12.

Zeroth-order light

Hexagonal bolt
(four locations)
Light-shielding plate
1st-order light
Ar mirror adjustment part
Clamp screw
(two locations)
190
4

Adjusting other lasers

1. To adjust the G-HeNe laser, 561-nm solid-state
laser or 594-nm solid-state laser, select the laser
wavelength with the numeric key on the AOTF
remote controller.
To adjust the G-HeNe laser, select L5 (543
nm).
To adjust the 561-nm solid-state laser, select
L6 (561 nm).
To adjust the 594-nm solid-state laser, select
L6 (594 nm).
(At the initial condition settings, the AOTF driver
is not adjusted well enough. Therefore the
transmitted light from the AOTF may be dark.)

Figure 12.3-20

2. Loosen the clamp screw of the spring of the L4
dichroic mirror adjustment part. And then, detach
the spring.

Figure 12.3-21

3. Loosen the clamp screw of the L4 dichroic mirror
adjustment part body. And then, detach the
dichroic mirror adjustment part body.

Figure 12.3-22

Precautions:
At the detachment of the dichroic mirror
adjustment part, the collimation pin may
come with the dichroic mirror adjustment part
body. Be careful to prevent the pin from
getting lost.

4. For the G-HeNe laser, 561-nm solid-state laser
or 594-nm solid-state laser, attach the centering
tool to the collimation pin after detaching the
dichroic mirror adjustment part. Rotate the
centering tool so that the laser light enters the
hole of the centering tool at a right angle. Adjust
the beam shift part so that the laser light enters
the Hole L.

Figure 12.3-23
191

5. Reattach the dichroic mirror adjustment part and
attach the centering tool to Position B shown in
the figure at right. Adjust the dichroic mirror
adjustment part in both horizontal and vertical
directions so that the laser light enters the Hole
L.

Figure 12.3-24

6. As for the 405 (440)-nm laser and 638-nm laser,
do the same as in the case of L4. Namely, attach
the centering tool to the collimation pin after
detaching the dichroic mirror adjustment part.
Adjust the beam shift part so that the laser light
enters the Hole L.

Figure 12.3-25

7. Reattach the dichroic mirror adjustment part and
attach the centering tool to Position B shown in
the figure at right.
Adjust the dichroic mirror adjustment part in both
horizontal and vertical directions so that the laser
light enters the Hole S (located at a distance of 1
mm left to the Hole L with respect to the AOTF
unit viewed from the front of the 4 laser unit A),
not the Hole L.

Figure 12.3-26

8. After adjustment, reattach the laser coupling
mirror part.
9. Select Line 3 (L3) on the AOTF remote controller
and change the Ar laser wavelength to 488 nm.

Figure 12.3-27
Position B
Centering
tool Hole L
Centering tool Hole L
Position B
Centering tool Hole S
Laser coupling mirror part
192

10. Attach the centering tool to position C shown in
the figure at right and adjust the laser coupling
mirror part in both horizontal and vertical
directions so that the laser light enters the Hole
L.

Figure 12.3-28

11. Tighten the clamp screws on the laser coupling
mirror part to secure it.

12. Restore the dichroic mirror adjustment part
detached in Step 3 to its original position. Then
fix it temporarily with a clamp screw.

Figure 12.3-29

13. Attach the spring of the dichroic mirror
adjustment part.

Figure 12.3-30

14. Manually open the L4 laser shutter.

15. Aim the laser light at the hole of the centering
tool attached in Step 3 in 3 Adjusting the Ar
laser position by rotating the setscrew for the
horizontal direction adjustment of the L4 dichroic
mirror adjustment part.

Figure 12.3-31
Position C
Centering tool Hole L
Laser coupling
mirror part
Clamp screws
Horizontal-direction setscrew
Centering tool
193

16. Fully loosen the clamp screw that fixes the
setscrew for the vertical direction adjustment of
the L4 dichroic mirror adjustment part.

Figure 12.3-32

17. Aim the laser light at the hole of the centering
tool attached in Step 3 in 3 Adjusting the Ar
laser position by rotating the setscrew for the
vertical direction adjustment of the L4 dichroic
mirror adjustment part.

Figure 12.3-33

18. Close the L4 laser shutter.

19. To adjust the 405-nm laser or the 638-nm laser,
select the laser wavelength on the AOTF remote
controller. And then, take the same steps for the
G-HeNe laser, 561-nm solid-state laser or
594-nm solid-state laser.
Rotate the centering tool l attached in Step 3 in
3 Adjusting the Ar laser position by 90
degrees so that the laser light from the 405-nm
laser or the 638-nm laser enters the centering
tool at a right angle.
The laser light must pass through the hole of the
centering tool.
Remove the centering tools after the adjustment.

Figure 12.3-34

Centering tool
Vertical-direction
setscrew
Centering tool
194
1

Adjusting the AOTF driver

Refer to the data sheet provided with the 4 laser unit A. Adjust and register the frequency and power
settings of the AOTF driver for the four wavelengths of the Ar laser, the wavelength of each of the
G-HeNe laser, 561-nm solid-state laser, 594-nm solid-state laser, 405-nm (440-nm) laser and 638-nm
laser.
In this section, an example case, the case for the 457-nm wavelength of the Ar laser is explained.

1. Select the Line 1 (L1) with the AOTF driver
remote controller.

3. Press the [1] key on the AOTF remote controller
and the display changes as shown in Figure
12.4-2.
Set the power to 18 dB on the AOTF remote
controller.
Subject the sensor of the power meter to the
laser light from the AOTF. Refer to the AOTF
data sheet provided with the 4 laser unit A and
adjust the frequency by pressing arrow keys
(</>) so that the laser power value of the power
meter becomes the maximum.
Keep pressing the arrow key to consecutively or
incrementally increase or decrease the figure.
Note: When performing frequency control, keep in
mind that if an arrow key is kept pressed, the
figure will increase/decrease very quickly after
the increase or decrease balance exceeds 2
MHz.
The frequency value on the data sheet is only a
guide. The optimum frequency value varies
depending on the incidence angle and
wavelength of the laser light emitted to the
AOTF.
When some steps of the frequency value cause
the same maximum power, the lowest frequency
value is used.
To select another wavelength number, press the
key assigned to it.

Figure 12.4-1

Figure 12.4-2

Figure 12.4-3
195

Remove the fiber coupling and attach the power
meter to the far end of the 4 laser unit A body
with a tape. It enables you to perform the AOTF
remote controller adjustment while measuring
the laser power.

Figure 12.4-4

Precautions
When some steps of the frequency value cause the same maximum power, the lowest
frequency value is used.
The 1st-order light transmittance of the 405-nm laser, 440-nm laser and 638-nm laser is lower
than that of other lasers; it is as low as approximately 65%.
The transmittance of the 488-nm Ar laser passed through the AOTF is approximately 50% of
the whole Ar laser light.
The transmittance of the 543-nm laser or 561-nm laser is approximately 90%.
In case the frequency adjustment has failed, a vertical stripe pattern may periodically appear
in a captured image, at the wavelengths of 543 nm and 561 nm in particular. In that case,
carefully readjust the frequency.

4. Subject the sensor of the power meter to the
laser light. Change the power value by pressing
the arrow keys (/) so that the laser power
value of the power meter becomes maximum.
Calculate the 99% value of the maximum. And
then adjust the power value so that the laser
power value of the power meter becomes the
99% value of the maximum.

Figure 12.4-5

Precautions
If the power value is too large, the linearity of
the brightness control will be affected. Be
sure to adjust the power value so that the
laser power value becomes the 99% value of
the maximum.

Attach the power meter
with a tape.
196

5. Press the STR key on the AOTF driver remote
controller after adjustments.
Settings are stored into the AOTF driver.
Press the ESC key to again display the
wavelength number for the laser beam to be
emitted. Another press on the key turns the
display to External mode.

Figure 12.4-6

Precautions
Be sure to press the STR key after each
adjustment to store the settings. The settings
will not take effect unless the STR key is
pressed.

6. Close the L3 laser shutter.

This is the end of the adjustment for the 457-nm laser.

7. Repeat Steps 1 to 4 for other lasers; 477-nm Ar laser, 488-nm Ar laser, 514-nm Ar laser, G-HeNe
laser, 561-nm solid-state laser, 594-nm solid-state laser, 405-nm (440-nm) laser, and 638-nm laser.
For details about wavelengths and laser types for each Line, refer to 2 Connecting the AOTF
driver remote controller in Section 12.3, Laser Rough Adjustment 1.

2

Adjusting the laser coupling mirror part

Adjust the optical path of the Ar laser (488 nm). The optical path of the Ar laser is used as a reference.
Optical paths of other lasers are aligned with it.

1. Restore the laser coupling mirror part to its
original position and tighten temporarily the two
hexagonal bolts on the laser coupling mirror part.
(Refer to Step 2 in 3 Adjusting the Ar laser
position in Section 12.3, Laser Rough
Adjustment 1.)

Figure 12.4-7
Tighten
temporarily
197

2. Attach the centering tool into the optical path at
the inlet of the optical fiber. Rotate the centering
tool so that the laser light enters the hole of the
centering tool at a right angle.
3. Manually open the L3 laser shutter. (The laser
shutter at the incident side of the mirror part
must be opened manually too.)

Figure 12.4-8

4. Select the Line 3 (L3) with the AOTF remote
controller to use the 488-nm Ar laser.

5. Insert a screwdriver into the adjustment hole for
the horizontal direction adjustment on the laser
coupling mirror part. And pry it to adjust the laser
light position in the horizontal direction.
Aim the laser light at the hole of the centering
tool attached in Step 2 by moving the
screwdriver.

Figure 12.4-9

6. Slightly loosen the two clamp screws for the
vertical direction adjustment on the laser
coupling mirror part.
7. Insert a screwdriver into the adjustment hole for
the vertical direction adjustment on the laser
coupling mirror part. And pry it to adjust the laser
light position in the vertical direction.
Aim the laser light at the hole of the centering
tool attached in Step 2 by moving the
screwdriver.

Figure 12.4-10
Laser shutter at the incident
side of the mirror part
Mirror reflector Centering tool
Horizontal
adjustment hole
Vertical adjustment hole Clamp screws
198

8. Tighten two hexagonal bolts on the laser
coupling mirror part and two clamp screws
loosened in Step 6 after the adjustments.

Figure 12.4-11

9. Close the L3 laser shutter and the laser shutter
at the incident side of the mirror part.

3

Aligning Each Laser Beam to the Ar Laser Beam

In this section, the G-HeNe laser and the 561-nm solid-state laser are described as examples.

1. Put a target such as a piece of paper at 50 cm or
farther away from the outlet of the laser of the
optical fiber coupling device.

2. Emit the Ar laser and the G-HeNe laser or
561-nm solid-state laser together by using the
AOTF remote controller.
To align the G-HeNe laser to the Ar laser:
Press the [3] and [5] keys in this order while
the screen of the AOTF driver remote
controller displays Internal mode. The
488-nm Ar laser and the G-HeNe laser are
emitted together.
To align the 561-nm solid-state laser to the
Ar laser:
Press the [3] and [6] keys in this order while
the screen of the AOTF driver remote
controller displays Internal mode. The
488-nm Ar laser and the 561-nm solid-state
laser are emitted together.

Figure 12.4-12

3. Manually open the L3 laser shutter and L4 laser
shutter.

Hexagonal bolts
199

4. Observe the two spots on the target. One spot is
the light of the Ar laser. The other spot is the
light of the G-HeNe laser or the 561-nm
solid-state laser. Adjust the L4 dichroic mirror
adjustment part so that the two spots converge.
(Do not touch any part of the Ar laser optical
path.) (For details about the adjustment, see
Steps 15 to 17 in 4 Adjusting other lasers in
Section 12.3, Laser Rough Adjustment 1.)

Figure 12.4-13

5. Observe the two spots on the mirror part. Two
spots here must converge to the same point, too.
Adjust the L4 beam shift part of the laser to be
aligned. (Do not touch any part of the Ar laser
optical path.) (For details about the adjustment,
see Steps 10 to 13 in 3 Adjusting the Ar laser
position in Section 12.3, Laser Rough
Adjustment 1.)

Figure 12.4-14

6. Repeat Steps 4 and 5. Laser spots on the target
and the mirror part must be aligned.
7. Close the L3 laser shutter and L4 laser shutter.
8. Repeat Steps 1 to 7 for the 405-nm laser and the
605-nm laser.

Align two spots.
Align two spots.
12 Setting up 4 Laser Unit A >> 12.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber
200
12.5 Final Adjustment for Transmitting Ar Laser Light through
1

Centering the Ar laser

1. Loosen three knurled screws at the optical fiber
coupling device.
2. Insert the coupler centering tool into the optical
fiber coupling device so that its pinhole side
faces the laser incident side.

Figure 12.5-1

3. Put a target such as a piece of paper away from
the laser outlet. Manually open the L3 laser
shutter and the L4 laser shutter.
4. Rotate the laser incident side adjustment screws
H and V to maximize the laser intensity.

Figure 12.5-2

Figure 12.5-3

5. Pull out the coupler centering tool once. And
then, insert the coupler centering tool into the
optical fiber coupling device so that its pinhole
side faces the laser outlet side.

Knurled screws
Coupler centering tool
Laser incident side
adjustment screw H
Laser incident side
adjustment screw V
201

6. Rotate the fiber side adjustment screws H and V
to maximize the laser intensity.

Figure 12.5-4

7. Repeat Step 5 and 6 to maximize the laser
intensity.

2

Attaching the fiber coupling

1. Close all laser shutters.
2. Attach the optical fiber coupling device. The tool
hole must face upward and the key groove must
be on the right viewed from the front side of the
fiber coupling device. Tighten three screws to fix
it.

Figure 12.5-5

Figure 12.5-6

screw H
screw V
Tool hole
Key groove
202
3

Transmitting Ar laser light through the single-mode optical fiber

WARNING

Never look directly at the fiber tip. Always monitor laser emissions by aiming the tip down onto
a sheet of paper.

1. Carefully align the end of the optical fiber (the
end without the tag) with the key groove in the
coupling and insert as far as it will go. Carefully
tighten the optical fiber connector. Gradually
tighten it. And stop when the laser light is
emitted from the tip of the optical fiber.

Figure 12.5-7

2. If no laser light is emitted from the optical fiber
even though the operation of Step 1 is
performed, do the followings:
Loosen the optical fiber connector about
10-degree angle and pull the optical fiber
carefully. Check if the laser light is emitted. (The
laser light will be defocused with this operation.
A laser light is emitted more easily if defocused.)
If it is not effective, repeat loosening the
connector and pulling the optical fiber a couple
of times.

Figure 12.5-8

3. If even a small amount of laser light is observed,
adjust the fiber side adjustment screws H and V
of the fiber coupling to maximize the laser
intensity.
4. Tighten the optical fiber connector gradually, and
stop the rotation just before the laser light
disappears.

Figure 12.5-9

5. Again, adjust the fiber side adjustment screws H and V of the fiber coupling to maximize the laser
intensity.
6. Repeat Step 4 and 5 to maximize the laser light intensity where the optical fiber connector is fully
tightened.
* If no light is emitted even after repeating these steps, repeat the procedure from 1 Centering
the Ar laser in Section 12.5, Final Adjustment for Transmitting Ar Laser Light through
Single-mode Optical Fiber.

screw H
screw V
203
4

Adjusting to give maximum Ar laser light transmission through the single-mode
optical fiber

Adjust to find the point giving maximum intensity, while measuring the Ar laser light intensity on the
power meter. To adjust intensity, rotate the beam shift part and the fiber side adjustment screws H and V
of the fiber coupling.
As a guideline for the efficiency of the Ar laser through the optical fiber, the intensity at the tip of the
optical fiber is at least 50% of the intensity before entering the optical fiber coupling device.

1. Attach the tip of the optical fiber onto the power
meter tool.

Figure 12.5-10

2. Rotate the whole L3 beam shift part by hands
and adjust the angle to get the maximum
intensity of the Ar laser light.
3. Adjust to find the point giving maximum intensity
of the Ar laser light by rotating the vertical axis
setscrew on the L3 beam shift part.

Figure 12.5-11

4. Rotate the fiber side adjustment screws H and V
of the fiber coupling to maximize the Ar laser
light intensity.

Figure 12.5-12

5. Repeat Step 2 to 4 to maximize the Ar laser
intensity.

screw H
screw V
Rotate by
hand
Axis A
204
5

Fixing the beam shift part and the optical fiber coupling device

1. Tighten the horizontal axis clamp screw and the
vertical axis clamp screw to secure the L3 beam
shift part.

Figure 12.5-13

2. Tighten the center knurled screw of the optical
fiber coupling device. And then, gradually tighten
two other knurled screws to fix the optical fiber
coupling device.

Figure 12.5-14

Knurled screws
Axis A
Rotate by
hand
Horizontal axis
clamp screw
12 Setting up 4 Laser Unit A >> 12.6 Final Adjustment for Transmitting Other Laser Light through Single-mode Optical Fiber
205
12.6 Final Adjustment for Transmitting Other Laser Light through
Laser lights other than the Ar laser have been adjusted by performing up to 3 Aligning each laser beam to the
Ar laser beam in Section 12.4, Laser Rough Adjustment 2. For the most part, other laser light passes through
the optical fiber at the completion of Section 12.5, Final Adjustment for Transmitting Ar Laser Light through
Single-mode Optical Fiber. Therefore, the adjustments for other laser lights are performed with the beam shift
part and the dichroic mirror adjustment part.

1

Adjustment to reach the maximum laser light transmissions through the optical
fiber

In this section, the G-HeNe laser and the 561-nm solid-state laser are described as examples.

1. Attach the tip of the optical fiber onto the power
meter tool.


3. Rotate the whole L4 beam shift part by hands
and adjust the angle to get the maximum
intensity of the laser light.
4. Adjust to find the point giving maximum intensity
of the laser light, by rotating the vertical axis
setscrew on the L4 beam shift part.

Figure 12.6-1

5. Rotate the horizontal axis setscrew on the L4
dichroic mirror adjustment part to maximize the
laser light intensity.

Figure 12.6-2
Rotate by
hand
Axis A
Horizontal axis setscrew
12 Setting up 4 Laser Unit A >> 12.6 Final Adjustment for Transmitting Other Laser Light through Single-mode Optical Fiber
206

6. Rotate the vertical axis setscrew on the L4
dichroic mirror adjustment part to maximize the
laser light intensity.

Figure 12.6-3

7. Repeat Step 3 to 6 to maximize the laser intensity.

8. Repeat Step 1 to 7 for the 405-nm laser and the 605-nm laser.

2

Fixing the beam shift part and the optical fiber coupling device

vertical axis clamp screw to secure the L1, L2,
and L4 beam shift parts.

Figure 12.6-4

vertical axis clamp screw to secure the L1, L2,
and L4 dichroic mirror adjustment parts.
3. Record the power of each laser.

Figure 12.6-5

Axis A Horizontal axis
clamp screw
Clamp screws
12 Setting up 4 Laser Unit A >> 12.7 AOTF Driver Remote Controller Detachment
207
12.7 AOTF Driver Remote Controller Detachment

1. When all the adjustments have been made,
press the ESC key of the AOTF driver remote
controller once or twice to display External
mode on the screen.

Figure 12.7-1

2. Detach the AOTF driver remote controller. Laser
control by PC is now available.

Precautions:
Laser control by PC is not available when the AOTF driver remote controller screen does not display
External mode. Make sure that the screen displays External mode before starting the laser
control.

This is the end of the laser adjustments.

12 Setting up 4 Laser Unit A >> 12.8 Operation Check
208
12.8 Operation Check

1. If the laser to be mounted on the 4 laser unit A
has not been set up, set it with the C2SetupTool.
For details, see Section 14.4, Setup in Chapter
14, Setting up PC and Software.

Figure 12.8-1

2. Launch the operation tool software C2Tool; click
the [Control] toggle button and select the [Laser
Power] tab.
3. Uncheck the [Calibration] check box (not
selected).
4. Select % in the [Input] combo box.
5. Set [Power] of the [Lasers] group box to 100.0
(%).
6. Check the [Shutter] checkbox (selected) of the
[Lasers] group box to open the shutter of the
laser to be set.
7. Confirm that the selected laser beam comes to
the front of the fiber incident end.
8. Confirm that the selected laser beam is emitted
from the fiber emitter end.
9. Using the power meter tool, record the power of
each laser by the wavelength.

Figure 12.8-2

12 Setting up 4 Laser Unit A >> 12.9 Sheet Metal Cover Attachment
209

1. Check the inside of the laser unit.

2. Fix cables and optical fibers with cable bands
provided with the laser unit.

Figure 12.9-1

3. Install the sheet metal cover.

Figure 12.9-2

4. Attach each side panel and fix it with six screws
each. (The Nikon marking is indicated on the
back part.)

Figure 12.9-3

5. Affixation of the laser safety label (class 3B)
required when setting up C1 is not required.

Figure 12.9-4

This is the end of the setup procedure.

Nikon marking

210

When using the optional spectral detector (MHE43000), the following options must be installed to the controller.

CAUTION

Make sure that the Power switch on the rear of the controller is switched off before starting the
setup procedures described here.

13.1 Installing Spectral Board (Optional)
When using the system with the spectral detector (MHE43000), the spectral board and 12 V cable supplied with
the spectral detector must be installed.
1. Pull out the power cable of the controller.

Precaution:
When the power switch on the rear of the controller is on, power is supplied to the primary source
(stand-by state). Never attempt to insert/remove a board under this state.

2. Inserting the spectral board (supplied with MHE43000)
1) Detach the side panel of the controller.
2) Remove the PCI bracket of slot 4 (the fourth slot from the top).
3) Insert the spectral board into slot 4.
4) Secure the PCI bracket part of the spectral board with the screw.

Figure 13.1-1 Inside of the controller

13 Setting up Controller

PCI bracket
Spectral board
13 Setting up Controller >> 13.1 Installing Spectral Board (Optional)
211
3. Connecting the four boards using the SYNC cable
Connect the available connector of the SYNC cable (10-wire flat cable) that connects between the
synchronization IO board, 1st line grabber board, and 2nd line grabber board, to J3 on the spectral board.
4. Connecting the 12 V cable (supplied with MHE43000)
Connect the unconnected power connector extending from the ATX power supply and the J4 connector on
the spectral board using the 12 V cable supplied with the spectral detector.

Figure 13.1-2

Synchronization IO board
Second line-grabber board
First line-grabber board
Spectral board
J4
Power connector 12 V cable
SYNC cable

212

14.1 D/A Board Installation [Only when AOM Unit or 4 Laser Unit A
Is Used]
The following procedure describes how to attach the PCI expansion board (National Instruments made, PCI-6711,
PCI-6713, or PCI-6733) into the PC. This board is used for controlling the 4 laser unit A or the AOM unit.
1. Start the PC and run the Windows.
2. Install the driver software of PCI-6711, PCI-6713, or PCI-6733 from the driver CD supplied with the
PCI expansion board.
3. Shut down the PC.
4. Insert the PCI expansion board (PCI-6711, PCI-6713, or PCI-6733) into a free PCI slot in the PC.
5. Start the PC and run the Windows again.
6. Double-click on the [Measurement & Automation] icon on the desktop to start the Measurement &
Automation Explorer.
7. In the left pane of the Measurement & Automation Explorer window, select the arrow at the left of
[Device and Interfaces] under [My System] and expand the sub-tree.
8. Check that the target PCI-6711, PCI-6713, or PCI-6733 is displayed under [Device and Interfaces].
Also check that "Dev1" is appended after the displayed board name.

Figure 14.1-1

Precautions:
If the target PCI-6711, PCI-6713, or PCI-6733 is not displayed under [Device and Interfaces], execute
[Refresh] in the [View] menu of the Measurement & Automation Explorer window. If it still is not
displayed, restart your PC and repeat from Step 6.
If the suffix of the displayed board name is not "Dev1", change it to "Dev1".

14 Setting up PC and Software
14 Setting up PC and Software >> 14.2 Network Settings
213
14.2 Network Settings
Two network cards are supplied with the PC. Specify network settings for one of the network cards to
communicate directly to the C2 controller with the cross-wired cable.
1. Select [Control Panel] from the Start menu. Select [Network and Internet] [Network and Sharing
Center].

Figure 14.2-1

The Network and Sharing Center window appears.
2. Select [Change adapter settings] and check the adapter names to confirm the network card connected
to the C2 controller. Right-click on the adapter, and then select [Properties] from the pop-up menu.

Figure 14.2-2

The Properties window appears.
14 Setting up PC and Software >> 14.2 Network Settings
214
3. Select [Internet Protocol Version 4 (TCP/IPv4)] and click the [Properties] button.

Figure 14.2-3

The Internet Protocol Version 4 (TCP/IPv4) Properties window appears.
4. Select the [Use the following IP address:] radio button, and set the IP address and subnet mask as
follows:
IP address 192.168.255.253
Subnet mask 255.255.255.248

Figure 14.2-4

5. Click the [OK] button to determine the change of the IP address.

14 Setting up PC and Software >> 14.3 Software Installation
215
14.3 Software Installation
For performing the setup, both the application and the setup tool software will be necessary.

14.3.1 Application Software
Obtain the installer of the NIS-Elements, and start the installer.
When the installation wizard screen is displayed, perform installation following the instructions.
For details, see NIS-Elements Advanced Research Users Guide.

14.3.2 Setup Tool Software
Install the following three tool software to setup the C2 system. For performing the installation, the administrative
privileges are necessary.
System parameter XML file creating software, C2SetupTool
*1

System calibration parameter setting software, C2CalibrationTool
*2

System simple operation tool software, C2Tool
*3

1. Obtain the C2SetupTool, the C2CalibrationTool, and the C2Tool, and perform installation.
2. Double-click on C2SetupTool.msi to install the C2SetupTool, C2CalibrationTool.msi to install the
C2CalibrationTool, and C2Tool.msi to install the C2Tool.
The installation wizard screen appears. Perform installation following the instructions on the screen.
*1: The C2SetupTool is a tool to create and modify the C2 system parameter XML file.
*2: The C2CalibrationTool is a tool to align the lasers or calibrate the PMT offset.
*3: The C2Tool is a tool to perform simple control of the hardware.
To start the C2CalibrationTool or the C2Tool, the NIS-Elements needs to be installed.

14 Setting up PC and Software >> 14.4 Setup
216
14.4 Setup
Create parameters necessary for the system using the C2SetupTool, and export the contents in a XML file format
used on the NIS-Elements.

14.4.1 Launching C2SetupTool
To launch the C2SetupTool, double-click on the C2SetupTool shortcut created on the desktop. The main menu
screen shown below appears.
Select work items, and start with clicking the [>>] button.

Figure 14.4-1

Select [New Setup] for a new setup.
Select [Version Up] for upgrading the NIS-Elements.
Select [Modify Configuration] for changing the configuration.

217
14.4.2 Hardware Configuration Setting
When [New Setup] or [Modify Configuration] has been selected in the select work items screen, the following
screen is displayed. When [Version Up] has been selected, the confirmation screen is displayed. See Section
14.4.5, Confirmation Screen.
In this screen, basic hardware configuration is set.

Figure 14.4-2

The contents of the setting are as follows:

Table 14.4-1
Confocal Type Select [C2plus].
Laser Unit Type Specify the type of the connected laser unit.
3 laser unit EX without the AOM: Select [3 Laser]
3 laser unit EX: Select [3 Laser + New AOM]
4 laser unit A: Select [LU4A]
Standard Detector Specify the combination of PMT. Only [3ch (2Grabber)] can be selected, and cannot be changed.
Transmitted Detector Specify the existence of the diascopic detector and the control method (motorized only).
Spectral Detector Specify the existence of the spectral detector.
MicroscopeType Select the microscope to use (only when the spectral detector is used).
Selection of the microscope here is for calibrating the transmittance of spectral detector and not
meant to control the selected microscope. Selection of the microscope to control is set on the
NIS-Elements.

When setting is complete, continue by clicking the [>>] button.

218
14.4.3 Laser Information Setting
In this screen, the laser information is set.
Depending on the state of the unit selected in the hardware configuration setting, necessary setting items are
displayed.
When [New Setup] has been selected in the select work items screen, the default is displayed.

For the 3 laser unit EX (with AOM)

Figure 14.4-3

1. Set whether the laser is enabled or disabled by checking the check box.
2. When you click the [] button of each laser, the [Laser Setting] dialog shown below opens to enable
you to set the details.

Figure 14.4-4

3. If modification is necessary, click the [] button of [Device Control File], and select an appropriate
device control file.
4. If modification is necessary, click the [] button of [Calibration File], and select an appropriate
calibration file. If calibration is not necessary, uncheck the [Calibration] checkbox (not selected).
Note: The laser wavelength measured for spectral detector is reflected to the XML file when the value
is entered in the [WaveLength] textbox.

219
Table 14.4-2: Selectable lasers and their wavelength (3 Laser + New AOM)
Wavelength Calibration File Device Control File Channels
408.0 nm 3LU-2 Melles & Griot 408 (56 ICS) on Nikon AOM
Controller.cal
Melles & Griot on Nikon AOM
Controller.edd
4
488.0 nm 3LU-2 Nikon AOM 488.cal Nikon AOM.edd 1
640.0 nm 3LU-2 Melles & Griot 640 (56 ICS ) on Nikon AOM
Controller.cal
Melles & Griot on Nikon AOM
Controller.edd
4


220
For the 4 laser unit A

Figure 14.4-5

1. Set whether the laser is enabled or disabled by checking the check box.
2. When you click the [] button of each laser, the [Laser Setting] dialog shown below opens to enable
you to set the details.

Figure 14.4-6

3. If modification is necessary, click the [] button of [Device Control File], and select an appropriate
device control file.
4. If modification is necessary, click the [] button of [Calibration File], and select an appropriate
calibration file. If calibration is not necessary, uncheck the [Calibration] checkbox (not selected).
Note: The laser wavelength measured for spectral detector is reflected to the XML file when the value
is entered in the [WaveLength] textbox.

221
Table 14.4-3: Selectable lasers and their wavelength (LU4A)
Shutter Wavelength Calibration File Device Control File Channels
1 637.0 nm Nikon AOTF 640.cal Nikon AOTF New.edd 8
440.0 nm Nikon AOTF 440.cal Nikon AOTF New.edd 1
3-1 457.9 nm Nikon AOTF 457.cal Nikon AOTF New.edd 2


222
14.4.4 Scan Setting
In this screen, the scan information is set.
By specifying the option to change the scanning direction of the scanner, up and down as well as left and right of
the images to obtain can be changed.
Set the following two options so that the images are the same as those obtained by binocular observation.

Figure 14.4-7


223
14.4.5 Confirmation Screen
Of the set information, major information is displayed.
1. Confirm the setting.
2. Click the [Apply] button to save the system parameter file.
3. The C2SetupTool exits after saving the setting information.
If ConfocalConfig.xml already exists, a backup file is created and saved.

Figure 14.4-8

14 Setting up PC and Software >> 14.5 System Adjustment
224
14.5 System Adjustment
Adjust the parameters specific to the unit using the C2CalibrationTool and export the contents in the XML file
format used on the NIS-Elements.
Note 1: Complete the system setup with the C2SetupTool before starting the C2CalibrationTool. If the XML file
has not been created with the C2SetupTool, the process using the C2CalibrationTool cannot be
executed.
Note 2: Complete the installation of the NIS-Elements before starting the C2CalibrationTool.

14.5.1 Starting C2CalibrationTool
1. Turn on the power of the laser.
2. Turn on the power of the C2 controller.
3. Double-click on the [C2CalibrationTool] icon on the desktop to start the C2CalibrationTool.
4. The main screen for calibrating lasers shown below appears.

Figure 14.5-1

Note: If communication with the C2 controller cannot be established, an error message appears and the
C2CalibrationTool exits.
Construct the environment so that communication with the C2 controller is established and restart the
C2CalibrationTool.

225
14.5.2 Checking/Modifying/Creating Device Control Parameter Setting
Checking, modifying, and creating the device control parameter setting are available.

Figure 14.5-2

1. In the [Select Laser] combo box, select a target laser.
2. The currently set device control file is shown in the [File] combo box of the [Device Control
Information] group box.
<A> Changing to another device control parameter file
1) In the [File] combo box, select the target device control file from the pull down list.
2) The [Save] button of the [Laser Calibration File Information] group box blinks in green.
3) Click the [Save] button to overwrite and save the changes.
<B> Changing the set values
1) Change any value of [Reference], [Dark Limit], [Bright Limit], or [Default Risetime] as
necessary.
2) The [Save] button blinks in green.
3) Click the [Save] button to overwrite and save the changes.
<C> Creating a new device control parameter file
1) Directly enter any file name in the [File] combo box.
2) The [Save] button blinks in green.
3) Determine the each value of [Reference], [Dark Limit], [Bright Limit], and [Default Risetime].
4) Click the [Save] button to create a new file.
5) Subsequently, the [Save] button of the [Laser Calibration File Information] group box blinks
in green.
6) Click the [Save] button to save the changes.
226
14.5.3 Checking/Modifying/Creating Laser Calibration Setting
Checking, modifying, and creating the laser calibration setting are available.

Figure 14.5-3

1. In the [Select Laser] combo box, select a target laser.
2. The currently set calibration data is shown in the [Laser Calibration File Information] group box.
<A> Changing the set values
1) Select a row to be changed from the calibration data list.
Values of the selected row are displayed in [Voltage] and [Reading].
Clicking the [Add] button adds a new row and clicking the [Remove] button deletes the
selected row.
2) Clicking the [Set DAC] button turns the button red and the laser is emitted with the power
voltage set in [Voltage]. Measure the power of the laser using the power meter, and enter
the reading value to [Reading].
3) Clicking the [Apply] button saves the entered values in [Voltage] and [Reading].
4) Repeat the steps from 1) as necessary.
5) Click the [Save] button to save the changes.
<B> Creating a new calibration data
1) Directly enter any file name in the [File] combo box.
(Click the [Create] button to display "New.cal" in the [File] combo box. In the calibration data
list, the range of voltage from [Dark Limit] to [Bright Limit] in the [Device Control Information]
group box is divided into 21 steps and is set automatically.)
2) Follow the steps in <A> Changing the set values, correct the rows as necessary.

Calibration data list
14 Setting up PC and Software >> 14.6 System Simple Operation
227
14.6 System Simple Operation
You can control the C2 system, adjust the power of each laser, and perform simple image capturing with the
C2Tool. You can check the post-setup operation of the C2 system with these functions.
Note 1: Complete the system setup with the C2SetupTool before starting the C2Tool. If the XML file has not been
created with the C2SetupTool, the process using the C2Tool cannot be executed.
Note 2: Complete the installation of the NIS-Elements before starting the C2Tool.

14.6.1 [Live] Tab: Image Capturing Tool
This tab screen can be used to connect to the C2 controller using the XML file created with the C2SetupTool to
obtain and display the image data without the NIS-Elements.
You can check the post-setup operation of the C2 system.

Figure 14.6-1

1. Turn on the power of the laser.
2. Turn on the power of the C2 controller.
3. Double-click on the [C2Tool] icon on the desktop to start the C2Tool.
4. Click the [Control] toggle button and select the [Live] tab.
5. Specify values in [Direction], [Size], [Speed], [Zoom], and [Angle] of the [Scanner] group box as
necessary.
6. Select an appropriate pinhole size in the [Pinhole] group box.
7. Check the [Ch] checkbox (selected) to specify the channel for capturing images.
8. Specify the wavelength of excitation laser used for each channel in the [Laser] combo box.
9. Clicking the [Live] button starts image capturing. A window in which the image data is displayed is
newly created and displayed.
228
10. Adjust the slider knobs of the [Laser] and [Gain] to get the image properly displayed.
11. Clicking the [Stop] button stops image capturing.
Note: In case of overly gained PMT, the [Scanner] button blinks. Release it by clicking.

14.6.2 [Laser Power] Tab: Controlling Laser Power
This tab screen can be used to connect to the C2 controller using the XML file created with the C2SetupTool to
control the laser power.
You can control the laser power at the time of laser unit adjustment.

Figure 14.6-2

1. In the [Calibration] checkbox, select if control of the laser using the calibration data is performed.
2. Determine the unit for the set values of laser power in the [Input] combo box.
3. Control opening/closing of the shutter of the corresponding laser in the [Shutter] checkbox.
4. Determine the laser output value in [Power]. Laser output values will fluctuate in the unit specified in
the [Input] combo box.

229
14.6.3 [Detail Setting] Tab: Checking/Setting Details of XML File
This tab screen can be used to check and set the XML file created with the C2SetupTool in detail.

Figure 14.6-3

230
The parameters that can be set in the [Detail Setting] tab of the C2Tool are shown below.

Table 14.6-1
Tab Parameter Details
General
Communication
Controller IP Address
IP Address on the unit side
Default setting: [192.168.255.254]
Communication
Timeout
Connection timeout with the unit side
Default setting: [10000] ms
Hardware Polling
Interval Time
Interval to confirm operation with the unit side
Control Server Heartbeat Interval Time
Interval of heartbeat of unit controlling server program
Write Event Log
Whether or not to output event log
Default setting: [ON]
Lasers
Laser Unit Type Selection of laser unit
Ni Card Device ID of D/A board to be used
Time of until shutter open
Shutter open/close time
Lasers
Wavelength Wavelength of lasers
Calibration Files
Specify whether to calibrate each laser and calibration file to
use
Device Control Files Specify control parameter definition files for each laser
Channels
Channel specification of D/A board corresponding to each
laser
Power Monitor
Average
Average times of laser power
Default setting: [8]
Interval Time
Measuring interval of laser power
Retrace Mask Enable Whether to use return mask
Detector
PMT Set
Combination setting of standard detector and transmitted
detector
PMT
Detector Count Number of detectors
Line Grabber Ch
Channel specification of Line Grabber corresponding to each
PMT
Type Specify the type of each PMT
Default Offset
Offset value of each PMT
Default setting: [0]
Linear Gain Factor
Correction parameter of Gain setting of each PMT
Default setting: [A=7.0, B=-12.5]
Spectral Detector
Whether or not spectral detector presents
Default setting: [OFF]
231
Tab Parameter Details
Scanner
Direction Specify scanning direction of scanner
Park Position: Escape
Whether to perform evacuation process in galvano mirror
park process
Offset
Normal
Offset value used as reference in normal scanning
Default setting: [0.00]
High
Offset value used as reference in high-speed scanning
Bi256
Offset value used as reference in normal bi-directional
scanning (resolution: 256x256)
Bi512
Offset value used as reference in normal bi-directional
HighBi256
Offset value used as reference in high-speed bi-directional
HighBi512
Offset value used as reference in high-speed bi-directional
Scan Delay Time
Initial/Manual
Specify setting method
Default setting: [Initial]
Normal
Scanning delay adjustment time used as reference in normal
scanning
Default setting: [60] s
High
Scanning delay adjustment time used as reference in
high-speed scanning
Bi256
bi-directional scanning (resolution: 256x256)
Bi512
bi-directional scanning (resolution: 512x512)
HighBi256
high-speed bi-directional scanning (resolution: 256x256)
HighBi512
high-speed bi-directional scanning (resolution: 512x512)
Pinhole Break Delay
Time
Initial/Manual
Specify setting method of pinhole delay time
Default setting: [Initial]
CW
Set pinhole delay time in CW rotation direction
CCW
Set pinhole delay time in CCW rotation direction
Other
Microscope Type
Specify the microscope (Used in Si transmittance calibration
process)
Trigger
IN Enable
Whether to use external trigger IN
IN Polarity
Specify external trigger IN logic
Default setting: [Negative]
OUT Enable
Whether to use external trigger OUT
OUT Polarity
Specify external trigger OUT logic
Default setting: [Negative]
OUT Interval Time
Delay time from external trigger signal input

232

CAUTION

The scan head and its controller are adjusted in combination. Make sure that the scan head and
controller being used have the same serial number.
Turn off the power switches of all devices when connecting cables.

1. Connect cables between units, as shown in Figure 15.0-1 to 15.0-9 and Table 15.0-1.
Cables to be connected to a unit are enclosed in each equipment package. The single-mode optical fiber
and multi-mode optical fiber to be connected to the scan head are enclosed in equipment package of scan
head.
2. Connect the LAN connector on the controller and the LAN connector of the PC (1-Gbit Ethernet) with the
optional cross-wired cable for 1000BASE-T.
3. Connect the power cables (optional) to the units that need power.

Figure 15.0-1 Controller

Figure 15.0-2 PC

Figure 15.0-3 3 laser unit EX

Figure 15.0-4 AOM controller
15 Cable Connections between Units
L1 L2
A1 A2 A3 A4
A5
A6
C1
C2
C3
C4
C5
C6
C17
C11
C7
C8
C9
C10
C13
C14
C15
C16
C12

P1
P2
P3
233

C2 scan head C2si scan head
Figure 15.0-5

Figure 15.0-6 4 laser unit A

Figure 15.0-7 Detector unit

Figure 15.0-8 Optical path selector part
of the diascopic detector

Figure 15.0-9 Spectral detector

S5
S4
S3
S2
S1
S6
4LA6
4LA5
4LA4
4LA3 4LA2 4LA1
4LA7
D2 D3 D4 D5
D1
TD1
TD2
SP1
SP2
234
Table 15.0-1 Table of Cable Connections (sheet 1 of 3)
Unit
Connector
No.
Connector
Name
Connect to
(Unit)
Connect
to (No.)
Connect to
(Name)
Remarks
Controller C1 Detector Detector unit D3 CONT
C2 3Laser/3EX 3 laser unit EX L2
C3 3Laser/AOM AOM controller A2 C1-SYNC
C4 Head/X Scan head C3 X
Galvano pair signal for
X-axis galvano scanner
control
C5 Head/Y Scan head C4 Y
Y-axis galvano scanner
control
C6 Head/I/O Scan head C5 I/O
C7 ILK
Connect the
shorting plug

Trinocular tube is used
with 80i/90i, Ni-E/U, FN1
When 4 laser unit A not
used
DIH ILK
DIH is used with 80i/90i
used
Quadrocular tilting
tube
INTERLOCK
Quadrocular tube is used
with Ni-E/U or FN1
used
Ergonomic tube LS
(Connect the
cable extending
out of Ergonomic
tube)
Ergonomic tube is used
with AZ100
used
Ti-E/U INTERLOCK
Ti-E/U is used
used
4 laser unit A 4LA4 CONF When 4 laser unit A used
C8 4LU 4 laser unit A 4LA3 CONTROLLER
C9 R Detector unit
D2 PMT
Ch1
C10 B Detector unit Ch3
C11 G Detector unit Ch2
C12 W Detector unit ChT
C13 (Spectral board) Spectral detector SP2 SIGNAL OUT Spectral board
C14 Ext Trg/Out External device Trigger output
C15 Ext Trg/In External device Trigger input
C16 LAN PC P2 (LAN) For communication
C17 AC IN AC power input

235
Unit
Connector
No.
Connector
Name
Connect to
(Unit)
Connect
to (No.)
Connect to
(Name)
Remarks
Scan head
C1 (Optical fiber) Laser unit
(FC)
C2 (Optical fiber) Detector unit D1 (Optical fiber)
(SMA)
C3 X Controller C4 Head/X
X-axis galvano scanner
control
C4 Y Controller C5 Head/Y
Y-axis galvano scanner
control
C5 I/O Controller C6 Head/I/O
C6 (Optical fiber) Spectral detector SP1 (Optical fiber)
(FC)
Detector
unit
D1 (Optical fiber) Scan head C2 (Optical fiber)
(SMA)
D2 PMT Controller C9-C12 R, G, B, W Ch1-Ch3, ChT
D3 CONT Controller C1 Detector
D4 T-PMT
Diascopic
detector unit
TD2
D5 (Light guide)
Diascopic
detector unit
TD1 (Light guide)
Optical path
selector part
of diascopic
detector
TD1 (Light guide) Detector unit D5 (Light guide)
TD2 Detector unit D4 T-PMT
Spectral
detector
SP1 (Optical fiber) Scan head C6 (Optical fiber)
(FC)
SP2 SIGNAL OUT Controller C13 (Spectral board)

236
Unit
Connector
No.
Connector
Name
Connect to
(Unit)
Connect
to (No.)
Connect to
(Name)
Remarks
4 laser unit
A
4LA1 AC IN AC power input
4LA2 AOTF/PC PC P3 (D/A card)
4LA3 CONTROLLER Controller C8 4LU
4LA4 CONF Controller C7 ILK Interlock signal
4LA5 MIC
Connect the
shorting plug

Trinocular tube is used
with 80i/90i, Ni-E/U, FN1
DIH ILK DIH is used with 80i/90i
Quadrocular tilting
tube
INTERLOCK
Quadrocular tube is used
with Ni-E/U or FN1
Ergonomic tube LS
(Connect the
cable extending
out of Ergonomic
tube)
Ergonomic tube is used
with AZ100
Ti-E/U INTERLOCK Ti-E/U is used
4LA6 TIRF Shorting pin jack
Supplied with the
LU4-C2 Connection Kit
4LA7 LUSU
Rectangular
shorting connector

Supplied with the
LU4-C2 connection kit
3 laser unit
EX
L1 AOM controller A6 AOM
L2 Controller C2 3Laser/3EX
AOM
controller
A1 AC IN AC power input
A2 C1-SYNC Controller C3 3Laser/AOM
A3 PC PC P3 (D/A card)
A4 LD-MOD Not used
A5 LD-PWR 405LD etc. Power control
A6 AOM 3 laser unit EX L1
PC P1 (AC IN) AC power input
P2 (LAN) Controller C16 LAN For communication
P3 (D/A card)
4 laser unit A 4LA2 AOTF/PC
3 laser unit EX A3 PC

237

The following adjustment and confirmation are performed by starting the system and using the NIS-Elements,
after completing the setup up to Chapter 15.

WARNING

Avoid looking at laser light emitted from the objective during scanning when you are in the
vicinity at the laser.
Since shading adjustment involves working under laser safety class 2 conditions, the lasers
must be handled as described below:
- When a G-HeNe laser is installed, use only this laser to make adjustments.
- When no G-HeNe laser is installed, use the Ar laser in STANDBY mode with the laser power
setting of approximately 10% of the ultimate intensity.
When making adjustments while observing the fluorescent image, use the specified objective
and make sure that the intensity of the laser light emitted has been suitably reduced before
starting. Use an adequately bright specimen, like an HE-stained specimen or a fluorescent
plastic specimen. The light should be reduced approximately as follows when using a bright
specimen:
- Ar laser: RUN mode, 20 mW, the laser power setting of approximately 5% of the ultimate
intensity
- G-HeNe laser: the laser power setting of approximately 30% of the ultimate intensity
Before starting this process, make sure the following:
- All of the sockets on the nosepiece are fitted with the dedicated tool for pupil observation, the
objectives, or the cover caps.
- The provided cap is in place on the rear port of the quadrocular tube when the upright
microscope Ni-E/U or FN1 is used without the DSC zooming port for quadrocular tube.
- A camera or a provided cap is in place on the camera port when the upright microscope FN1
is used with the FN-DP double port.
- A dia-illumination lamphouse or a cap is in place on the right side port when the inverted
microscope Ti-E/U is used.

CAUTION

These procedures involve the final system inspection and adjustment. Check once again that
the system is correctly assembled and connected before starting.

16 General Adjustment
16 General Adjustment >> 16.1 Adjustment of Scan Head Inclination
238
16.1 Adjustment of Scan Head Inclination
In order to minimize lower brightness (shading) in periphery of images, perform tilt adjustment of the scan head.
The adjustment procedure is slightly different depending on the microscope to use.

16.1.1 Microscope 80i/90i, Ni-E/U, or FN1
1. Preparation
1) As shown in Figure 16.1-1, set the centering tool for pupil observation on the nosepiece.
Place a mirror on the stage to enable observation of the centering tool.
2) Turn on the laser to use for adjustment. For laser safety reasons, low power HeNe laser (green, yellow,
or red) is appropriate.
3) For entering laser to the center of the optical axis of the objective, perform Live (scanning to obtain live
images) with the center of the field of view set to the maximum zoom magnification (1000x) on the
NIS-Elements.
4) Confirm that a circular pupil is projected onto the centering tool.

Figure 16.1-1

2. Adjustment
1) Referring to the concentric circles on the centering tool, note the extent and direction of the deviation
of the pupil position, as shown in Figure 16.1-2. (The centering tool is marked in 1-mm graduated
intervals.)

Figure 16.1-2

Centering tool for pupil
observation
Mirror
Pupil of the centering tool,
projected by the mirror (viewed
from the front of microscope)
Center is deviated one division (1
mm) in the direction a.
a
45 degree
All of the sockets on the
nosepiece should be filled up
with objectives or cover caps.
239

2) Determine the amount of inclination by shifting the origin index mark from the reference index mark on
the vertical tube by a distance corresponding to the deviation indicated on the tool. Loosen the
setscrew B, rotate the scan head and vertical tube mount according to the above scale indication.
Then tighten the setscrew B (see Figure 16.1-7).
Example: If the deviation as viewed from the front of the microscope is as shown in Figure 16.1-2, the
center is shifted 1 mm toward a, as illustrated in Figure 16.1-4. In this case, move the
origin index mark one division on the scale from the reference index mark, as shown in
Figure 16.1-6, and tighten the setscrew B.
If the deviation is 0.5 mm, move the origin index mark one half-division, and tighten the
setscrew.
3) As illustrated in Figure 16.1-6, the midpoint between the black reference index mark and the black
origin index mark represents the position of maximum inclination. As shown in Figure 16.1-5,
determine the inclination by rotating the entire vertical tube so that the midpoint between the two index
marks moves in the direction b, 180 degree opposite the direction of a.
Loosen the setscrews A and C. Holding the scan head in place with your hand, rotate the entire
vertical tube in the direction determined in the above step then tighten the setscrews A and C (see
Figure 16.1-7). Be careful not to damage the scan head cable.

Pupil image viewed from above

Top view of vertical tube

Rotate the entire vertical tube.
Move the midpoint between two index marks in
the opposite direction to a, and fix the vertical
tube.
For deviation of one division on
centering tool
Move the origin index mark one
division (1 mm) from the
reference index mark.


a
45 degree
a
Mount
Vertical tube
Origin index mark (black)
b
Reference index
mark (black)
Midpoint
between two
index marks
Mount
Vertical tube
Reference index mark (black)
Position of maximum
inclination (midpoint
between two index marks)
Setscrew B
Setscrew C
Setscrew A
240
3. Confirmation
1) Make sure that the deviation of the pupil position is 0.5 mm or less, as measured by the centering tool.
2) If the deviation is larger than 0.5 mm, loosen setscrews A and C. While holding the scan head with
your hand, slightly turn the entire vertical tube to perform fine adjustment.
If this adjustment fails to reduce the deviation to the specified level, repeat the procedure described in
Step 2, Adjustment.
3) When adjustment is complete, make sure all the setscrews are firmly tightened.

241
1. Preparation
1) Set the AZ100 as follows:
Zoom magnification : 3x
AS (aperture stop) : Fully open
Objective : Dummy
Elevating section : Lower to the end
EPI stage : Raise to the end
3) Remove the objective, put a sheet of white paper on the stage, lower the elevating section, and then
raise the EPI stage to make the white paper on the stage closer to the nosepiece mount.
NIS-Elements.
5) Confirm that the beam diameter of the laser beam irradiated on the white paper is circular shape.

2. Adjustment
1) After confirming zoom magnification of AZ100 is 3x, and the AS (aperture stop) is fully open, lightly
tighten the two setscrews in the Y direction of the eyepiece tube, and, by pushing and pulling the two
setscrews in the X direction, make adjustment so that the whole pupil enters in the AS (aperture stop)
projected on the paper.
2) Then, by pushing and pulling the two setscrews in the Y direction, make adjustment so that the whole
pupil enters in the AS (aperture stop). If not matching, the pupil will look as if a part of it is missing.
3) After adjusting the position of the pupil, simultaneously tighten and fix the setscrews in the X direction.
4) Then, simultaneously tighten and fix the setscrews in the Y direction.

Adjustment in the Y direction
Adjustment in the X direction
The status that the whole pupil is in the AS

242
1. Preparation
1) As shown in Figure 16.1-11, set the centering tool for pupil observation on the nosepiece.
In the case of the inverted microscope Ti-E/U, a mirror is not used, since the pupil position can be
observed directly.
NIS-Elements.
4) Confirm that a circular pupil is projected onto the centering tool.

Figure 16.1-11

2. Adjustment
1) Referring to the concentric circles on the centering tool, note the extent and direction of deviation of
the pupil position, as shown in Figure 16.1-12.

Figure 16.1-12

2) Determine the amount of inclination by shifting the origin index mark from the reference index mark on
the vertical tube by a distance corresponding to the deviation indicated on the tool.
Loosen the setscrews B and C, then, while manually supporting the scan head so that it is pressed
against the microscope side, rotate the vertical tube mount according to the above scale indication.
Tighten the setscrew B (see Figure 16.1-17).
Example: If the center is shifted 1 mm in the direction of a as shown in Figure 16.1-12, the deviation
as viewed from the side is as shown in Figure 16.1-14.
In this case, move the origin index mark one division on the scale from the reference index
Centering tool for
pupil observation
All of the sockets
on the nosepiece
should be filled up
with objectives or
cover caps.
Pupil of the centering tool,
projected by the mirror (viewed
from the front of microscope)
Center is deviated one division (1
mm) in the direction a.
a
45 degree
243
mark and tighten the setscrew B as shown in Figure 16.1-15.
If the deviation is 0.5 mm, move the origin index mark a half-division and tighten the
setscrew.
3) The midpoint between the black reference index mark and the black origin index mark is the position
of maximum inclination. As shown in Figure 16.1-16, determine the direction of inclination by rotating
the entire vertical tube so that the midpoint between the two index in the direction b, 180 degree
opposite the direction of a when viewed from the side port.
Loosen the setscrews A and C. While manually supporting the scan head with your hand so that it is
pressed against the side port (see Figure 16.1-17), rotate the entire vertical tube in the direction
determined in the above step, then tighten the setscrews A and C (at the two upper locations).
Be careful not to damage the scan head cable.

Figure 16.1-13
Top stage surface image viewed from the side

Figure 16.1-14
Viewed from the side port

Figure 16.1-15


a
45 degree 1
Rotate the entire
vertical tube.
Mount
Vertical tube
b Midpoint between
two index marks
Reference index
mark (black)
Move the midpoint between two
index marks in the opposite direction
to a, and fix the vertical tube.
Position of maximum
inclination (midpoint
between two index marks)
Mount
Reference index
mark (black)
Vertical tube
For deviation of one division
on centering tool scale,
move the origin index mark
one division (1 mm) from the
reference index mark.
Setscrew A Setscrew B
Setscrew C
244
3. Confirmation
1) Make sure that the deviation of the pupil position is 0.5 mm or less, as measured by the centering tool.
2) If the deviation is larger than 0.5 mm, loosen setscrews A and C. While supporting the scan head with
your hand, turn the straight tube slightly to perform fine adjustment.
If this adjustment fails to reduce the deviation to the specified level, repeat the procedure described in
Step 2, Adjustment.
3) When adjustment is complete, make sure all the setscrews are firmly tightened.

16 General Adjustment >> 16.2 Adjustment of Condenser Lens in Front of Pinhole
245
16.2 Adjustment of Condenser Lens in Front of Pinhole
1. Preparation
1) Set the fluorescent plastic with a cover glass attached as a uniform fluorescent specimen to the
microscope.
2) Set a laser, the 1st dichroic mirror, and the filter cube appropriate for imaging the fluorescent plastic.
2. Adjustment
1) Obtain live images on the NIS-Elements in the following conditions.
Adjust the focus position to a place 20 m or more inside from the edge face of the fluorescent plastic,
where the brightness changes little even when the focus is changed.
<Image acquisition conditions for adjustment>
Scanning mode : 512 x 512
Field of view : Zoom 1x (Maximum)
Objective : 60x or higher
Pinhole size : M2 or smaller
2) Move two condenser lens adjustment screws gradually until the center of the image is at its brightest.
To obtain the value of the brightness at the center of the image, use the analysis tool provided in the
operation software NIS-Elements.
Method of obtaining brightness value
Using the analysis tool, position a circular spot with an area of about 32,000 pixels (i.e., a radius of
approx. 100 pixels) at the center of the image and display the average brightness.

Figure 16.2-1

Condenser lens
adjustment screws
(2 locations)
16 General Adjustment >> 16.2 Adjustment of Condenser Lens in Front of Pinhole
246
3. Confirmation
After adjusting the condenser lens in front of the pinhole as described above, confirm that there is no
noticeable shading (i.e., irregularities in brightness) and changing the focus results in concentric circular
blurring, not blurring in eccentric ways.
1) Obtain live images on the NIS-Elements in the following conditions.
Adjust the focus position to a place 20 m or more inside from the edge face of the fluorescent plastic,
where the brightness changes little even when the focus is changed.
<Image acquisition conditions for confirmation>
Scanning mode : 512 x 512
Field of view : Zoom 1x (Maximum)
Objective : PlanApo 60xWI (Correction ring must be adjusted.)
Pinhole size : M2
2) Measure shading (unevenness of brightness). For measuring, use the analysis tool of the
NIS-Elements.
How to determine shading
By using the analysis tool, put circular spots of approximately 720 total pixels (approximate radius is
15 pixels) on the center of the image as well as the four corners of the image to display the average
brightness.
3) If both conditions are not satisfied, repeat the procedures described in Chapter 6, Setting up Scan
Head or Section 16.1, Adjusting Scan Head Inclination.
Shading (unevenness of brightness) should satisfy the following specification:
- Ratio of average brightness value of the center of the image and the minimum value of the
four corners is 55% or higher.
- Ratio of average brightness value of the maximum value and the minimum value of the four
corners is 65% or higher.
Changing the focus should result in concentric circular blurring, not blurring in eccentric ways.
(Note: Check the tilting of the specimen.)

16 General Adjustment >> 16.3 Compensation of Image Offset for Bidirectional Scanning
247
16.3 Compensation of Image Offset for Bidirectional Scanning
Adjust the image offset for the bidirectional scanning by altering the sampling timing.
1. Preparation
1) Preparing a specimen
To make offsets easier to assess, prepare specimens with prominent vertical lines. A grid micro
specimen (reflection observation) is ideal.
2) Checking the scanning direction setting

CAUTION

Changing the scanning direction setting in the middle of adjustment will offset the
adjustment values. Make any changes in the scanning direction before adjusting.

Confirm that the scan setting described in Section 14.4.4, Scan Setting in Chapter 14, Setting up PC
and Software are correctly set.
Upright microscope: Set Scan Direction X and Scan Direction Y to Normal
Inverted microscope: Set Scan Direction X and Scan Direction Y to Reverse
2. Adjustment
Adjust using the combinations of 19 steps of zoom magnifications (five steps in high-speed scanning), two
steps of image sizes, and two steps of scanning speeds (48 combinations in total).

CAUTION

When adjusting with a reflected image using a grid micro specimen, remove all filter cubes
inserted in the detector unit and detect it using channel 3.

1) Image displacement correction in 512 size, 2FPS bidirectional scanning (delay time setting)
The images of the specimen prepared on the NIS-Elements are obtained in the following conditions.
Scanning mode : 512 x 512, Bidirectional scanning, 2FPS
Zoom : Magnification of 1 to 20 (19 scales); See the table shown below.

Table 16.3-1
Zooming magnification to be adjusted
1.000 1.054 1.111 1.176 1.250 1.333 1.429 1.538 1.667 1.818
2.222 2.500 2.857 3.344 4.000 5.000 6.711 10.000 20.408

248

Figure 16.3-1

When a bidirectional image is shifted on either side as shown in Figure 16.3-3, click the [Direction
mismatch adjustment] button (for correcting image shift) on the Scan setting dialog, in the C2plus
settings window of the NIS-Elements, make changes so that the image shift becomes minimum using
the [Galvano pixel gap] slider bar (for correcting bidirectional scanning) shown in Figure 16.3-2. Adjust
this with the 19-step zoom magnification.
This value will be stored automatically in the configuration file (XML).

Figure 16.3-2

Figure 16.3-3

2) Image displacement correction in 256 size, 4FPS bi-directional scanning (delay time setting)

Zoom
Bidirectional scanning Scan size
Scan speed
Fast scanning
249
Table 16.3-2
1.000 1.054 1.111 1.176 1.250 1.333 1.429 1.538 1.667 1.818
2.222 2.500 2.857 3.344 4.000 5.000 6.711 10.000 20.408

Adjust with the 19-step zoom magnification in the same way as 1).

3) Image displacement correction in 512 size, 3FPS bi-directional (high-speed) scanning (delay time
setting)
Click the [Fast] button to switch to the fast scanning.

Table 16.3-3
4.000 5.000 6.711 10.000 20.408

Adjust with the five-step zoom magnification in the same way as 1).

4) Image displacement correction in 256 size, 6FPS bi-directional (high-speed) scanning (delay time
setting)
Click the [Fast] button to switch to the fast scanning.

Table 16.3-4
4.000 5.000 6.711 10.000 20.408

Adjust with the five-step zoom magnification in the same way as 1).

16 General Adjustment >> 16.4 Microscope Settings for Diascopic DIC Microscopy
250
16.4 Microscope Settings for Diascopic DIC Microscopy
To observe the DIC image with the diascopic detector unit, the microscope must be equipped with the DIC Set.
See the setup manual for each microscope for information on DIC setup.
Note also that in the C2 system, the laser beam is emitted as a linear polarized light, in the preset direction
consistent with the DIC prism. Therefore, the microscopes analyzer is not used.
Use the following filter settings for the diascopic illumination system of the microscope.
Diffuser : Turn on when using in conjunction with the 90i/80i, Ni-E/U, FN1 or Ti.
NCB filter : To be removed
ND filter : To be removed under normal condition. However, they can be used for the brightness control
by adjusting the applied voltage if the image is too bright even after the gain is reduced by the
adjustment of the laser brightness or the switching of the objective.

CAUTION

The DIC images derived from this system should present more uneven brightness compared to
the DIC images of the usual DIC microscopes, especially when the low powered objective is
used. (It is recommended to fix the optical fiber for excitation light after setting up to avoid
stress on the fiber.)

Accessories required for the microscope
<90i/80i>
Condenser : D-CUD-E motorized universal condenser (dry), D-CUD universal condenser (dry)
Polarizer : D-DP DIC rotating polarizer
Nosepiece : D-ND6-E motorized DIC6 nosepiece, D-ND6 DIC6 nosepiece
DIC prism : DIC prism set
Objective : DIC objective

<Ni-E/U>
Condenser : NI-CUD-E motorized universal condenser dry, NI-CUD universal condenser dry
Polarizer : D-DP DIC rotating polarizer
Nosepiece : NI-ND6-E motorized sextuple DIC nosepiece, D-ND6 DIC6 nosepiece

<FN1>
Condenser : FN-C LWD condenser unit
Polarizer : FN-PT polarizer turret, FN-P polarizer
Nosepiece : FN-S2N slider nosepiece

16 General Adjustment >> 16.4 Microscope Settings for Diascopic DIC Microscopy
251
<AZ100>
Condenser : Built in the AZ100
Polarizer : AZ-RP rotatable polarizer
Nosepiece : AZ-NPS single nosepiece, AZ-NP3 triple nosepiece
DIC prism : AZ-DP DIA DIC prism, AZ-DPS DIA DIC prism slider
Prism holder : AZ-FLDIC FL-DIC prism holder
Objective : Applicable objectives to AZ100

<Ti>
Condenser : LWD condenser or high N.A. condenser
Polarizer : T-P2 polarizer
Nosepiece : TI-ND6-E motorized DIC6 nosepiece
DIC prism : Differential interference contrast condenser module, DIC slider

Adjusting the microscope
You need to adjust the focus and centering of your condenser by using the halogen light source according
to your microscopes manual.
Fully open the aperture diaphragm, but open the field diaphragm only in accordance with the target field of
view. (Stopping down the field diaphragm excessively will cause vignetting.)
Adjust the orientation of the polarizer using the analyzer of the DIC set so that they will be set in the
Crossed Nicols position when observed through the eyepieces.

16 General Adjustment >> 16.5 Accuracy Verification of Spectral Detector
252
16.5 Accuracy Verification of Spectral Detector
The accuracy of the spectral detector must be verified at the time of installation.

Caution for verifying the image and the accuracy with the spectral detector
To verify the image and the accuracy with the spectral detector, be sure to comply with instructions below.
If not, the accuracy will be inferior to the specifications.
Measurement conditions
Warm-up time: approx. 40 minutes
Temperature and humidity of the environment: 18 to 28C, 60%RH or lower (with no condensation)
Measurement wavelength: 420 to 750 nm [applicable wavelength: 400 to 750 nm]
PMT gain: 142 (-500 V) to 255 (-900 V) [programmable range: 144 (-400 V) to 255 (-900 V)]
Pixel dwell: 4 to 30 sec. [programmable range: 4 to 60 sec.]
The spectral detector unit requires you to set the actual wavelength of the laser to be used to the system
parameter in the NIS-Elements C. Because of the variation in oscillating wavelengths caused by individual
characteristics of each DPSS (solid-state) lasers and LD (laser diodes), the actual wavelength must be
measured
*1
.
For lasers that require measurement, see Table 16.5-1.
*1: Failing to measure the actual wavelength can lead to poor imaging quality caused by fluorescence
mixing with the laser light or even worse, degrade the performance of the PMT.
When verifying the wavelength accuracy, it is also necessary to measure the actual wavelengths of the gas
lasers (Ar and HeNe). The lasers usable for verifying the wavelength accuracy of the spectral detector must
meet the following conditions. When the measured values are within the range of the verified wavelength
accuracy, it is determined that the wavelength accuracy of the spectral detector is normal.

Table 16.5-1
Type
Measuring actual
wavelength
Wavelength (nm)
Usability for checking
wavelength accuracy of
spectral detector
Range of verified wavelength
accuracy
LD Required
Wavelengths listed
in Table 1.2-3

DPSS Required
Wavelengths listed
in Table 1.2-3
Usable for the laser whose
wavelength is within 0.5 nm of
the specification wavelength
listed in Table 1.2-3
The difference between measured
wavelength and specification
wavelength must be within 1.5 nm.
Gas
(Ar)
Required if verifying
wavelength accuracy
457.9
476.5
488.0
514.5
Usable
Gas
(HeNe)
Required if verifying
wavelength accuracy
543.5 Usable

253
If no laser that can be used for verifying the wavelength accuracy is installed in the system, verify the accuracy by
referring to Confocal Microscope A1, Setup Manual, Part 2, A1si QC Operation Manual.

Measuring wavelength of mounted lasers with NIS-Elements C and setting
wavelength of laser diodes and DPSS lasers
1. Verifying the accuracy of wavelength measurement with Multi Ar laser or HeNe laser
2. Measuring the actual wavelength of laser diodes and DPSS lasers
3. Setting the measured actual wavelength

Detailed steps
1. Verifying the accuracy of wavelength measurement with Multi Ar laser or HeNe laser
The accuracy of wavelength measurement is verified by measuring Multi Ar laser or HeNe laser.
To do so, the value of Cofocalconfig.xml must be temporarily overwritten to set the laser wavelength from
NIS-Elements to a value different from the actual value, and the laser shielding plate must be moved outside
the laser wavelength measurement range.
1) From the C2SetupTool, change the laser wavelength to be measured for accuracy verification.
Note: Change it to a value that is less than the actual wavelength principally.
Example: When measuring Multi Ar 488-nm laser: 488 -> 457
2) Attach a cap to the nosepiece of the microscope and bring that nosepiece hole into the optical path.
3) Measure the wavelength in the SD (Spectral Detector) mode.
For details on the SD mode, see Chapter 6, Detection Mode (Spectral Detector) in NIS-Elements C
(For CONFOCAL MICROSCOPE C2/C2si) Instructions manual.
Measurement condition: Manual mode, Resolution: 2.5 nm, Channel32,
Scan: one-way; Pinhole: Minimum; Zoom: 1; 1stDMBS 20/80
Keep [Laser Power] and [Si HV] set to 0 and adjust them gradually after starting measurement.
Adjust so that the signal strength does not exceed 1000.
4) Check the peak value with the Spectrum Profile.
Compare the measured laser wavelength and the peak value and check that it is within the range
defined in Table 16.5-1.
5) Restore the wavelength overwritten in step 1) to its original value.
2. Measuring the actual wavelength of laser diodes and DPSS lasers
Measure the actual wavelength of the laser diodes and DPSS lasers with the same method as in procedure
1.
Example: Set to 440.0 when measuring 408-nm laser diode.
Example: Set to 405.0 when measuring 440-nm laser diode.
3. Setting the measured actual wavelength
Modify the value of the laser diodes and DPSS lasers measured in Step 2 using the C2SetupTool.

254
For other accuracy verification and procedures, perform measurement referring to Confocal Microscope A1,
Setup Manual, Part 2, A1si QC Operation Manual.

255

1. Setup date/user
Setup date Month Date Year to Month Date Year
Work description (Summarize the
work when the work type is new
installation or maintenance.)
Work type
Summary
User name (Section/contact
person)

Location
Setup engineer
Sales representative

2. Configuration of each unit
2-1 Confocal units
Scan head S/N
Ring adapter
Standard detector unit S/N
Spectral detector unit S/N
1st dichroic mirror
Filter cube (2nd) (1)
Filter cube (3rd) (2)
Laser unit (4 laser unit) C-LU4A S/N
Laser 1 (Main unit, power supply) S/N
Laser unit (3 laser unit) C-LU3EX S/N
AOM unit Yes No S/N
AREX EX changer Yes No S/N
LU-LR laser rack Yes No S/N
Vibration isolated table Yes No
Note:

17 Confirmation and Check Sheet Entry
256
2-2 Brightness control
Z motor Model: S/N
RFA: Yes No
RFA adjusted
value:
S/N
Diascopic detector Yes (Motorized) No S/N
2-3 PC / software
Manufactural information of the
PC
Manufacturer: Model:
Serial number:
OS Windows SP( )
CPU GHz
HDD GB
Memory GB
Monitor Manufacturer: Model:
Control software NIS-Elements C Ver.
Option String (number of the license card)
PCI card (NI-DAQ) Model/Ver.
Additional LAN board Installed Not installed Other additionals:
2-4 Microscope
Model Microscope: Microscope stand serial number:
Ti HUB Ver. CPU (MAIN): Ver. CPU (SUB): Ver. CPLD (U52): Ver. S/N
T-RCP (Control pad) PAD CPU: Ver
Objective 1
2
3
4
5
6
7
Fluorescence filter cubes (if the
blank space is not enough, use
the Note:)
1: 2: 3: 4: 5: 6:

Turret Filter turret: Motorized Manual Objective turret Motorized Manual
Motorized TIRF Yes No Manufacturer/model S/N
Motorized stage Yes No Manufacturer/model S/N
Piezo function Yes No
Power Manufacturer/model S/N
Stage Manufacturer/model S/N
P.F.S. Yes No S/N
Note: Other peripheral devices

257
3. Adjustments to be checked
3-1 Laser alignment (LU4A) Check
Check Input at the fiber end Output at the fiber end Efficiency Before At setup
L1 ( nm) mW ( ) mW ( ) mW %
L2 ( nm) mW ( ) mW ( ) mW %
L3-1 ( nm) mW ( ) mW ( ) mW %
L3-2 ( nm) mW ( ) mW ( ) mW %
L3-3 ( nm) mW ( ) mW ( ) mW %
L3-4 ( nm) mW ( ) mW ( ) mW %
L4 ( nm) mW ( ) mW ( ) mW %
Requirement 1 The output value is higher than the specified output.
Requirement 2 The efficiency is 50% or higher; but it is to be 10% or higher for the 405 (408) nm laser.
3-2 AOTF tuning value Wavelength Frequency Power
L1 (7) 405 nm MHz dBm
L3-1 (1) 457 nm MHz dBm
L3-2 (2) 477 nm MHz dBm
L3-3 (3) 488 nm MHz dBm
L3-4 (4) 514 nm MHz dBm
3-3 Laser alignment (LU3EX) Check
Check Input at the fiber end Output at the fiber end Efficiency Before At setup
L1 ( nm) mW ( ) mW ( ) mW %
L2-1 ( nm) mW ( ) mW ( ) mW %
L2-2 ( nm) mW ( ) mW ( ) mW %
L2-3 ( nm) mW ( ) mW ( ) mW %
L3 ( nm) mW ( ) mW ( ) mW %
Requirement 1 The output value is higher than the specified output.
Requirement 2 The efficiency is 50% or higher; but it is to be 10% or higher for the 405 (408) nm laser.
3-4 Extinction ratio of polarized light
Requirement
With an Ar and linearly polarized light HeNe laser, the extinction ratio of the fiber output
light is 20:1 or higher.

Measured value: mW: mW Ratio : 1
3-5 Profile of the fiber output Check
Requirement The shape of the fiber output laser is concentric circles.
3-6 Shading Before At setup
Reading on the shading tool ( ) div.
Requirement 0.5 mm
3-7 Shading adjustment by using images Before At setup
Condition 1 Objective: 60x or more; Pinhole size: M2
Requirement ROI (an area of about 32,000 pixels) at the center of the image captured by 512512
scanning mode is the brightest.

Adjust the condenser lens to the brightest by checking [Add saturation indicator].
3-8 Check by using images Before At setup
Condition 1 Objective: Plan Apo 40X; Pinhole size: S2
Condition 2 Laser power is 25% (or ND4 pushed in) at 488-nm laser.
Brightness check By using the standard specimen 1, images have sufficient brightness with the gain 100.
Standard specimen 1 Trichrome stained specimen (product No. F-14781) manufactured by Molecular Probes, Inc.
3-9 Laser noise Before At setup
Requirement There is no noise observed on images with the 408 nm laser.
There is no noise observed on DIC images with the laser.
3-10 Laser output from the objective Before At setup
Condition With the maximum output of the lasers and the lowest magnification setting of the
objective, measure the intensity of the laser coming from the objective around the plane
of object.

Measured wavelength: nm Objective: x Power: mW

258
4. Spectral detector check
4-1 Wavelength accuracy check Check
Condition 1 Set to use the mercury lamp, wavelength resolution to 2.5 nm, and channel to 32.

Set the start wavelength to 500 nm (disable all lasers).
Condition 2 Create spots across the Live window. Set the field of view to 0 m, pixel size to 256256,
pinhole to L. Measure the peak of wavelength.

Condition 3 Use the GFP/FITC cube without EX.
Position of the wavelength peak At 546 nm (the peak is between 546 and 547).
4-2 Wavelength accuracy check Check
Laser shield position The wavelength of the laser specified on the spectral graph is on the position.
4-3 Photomultiplier characteristics evenness check Check
Condition 1 Heat run for 2 hours or longer, keeping the halogen lamp turned on.
Condition 2 Disable all lasers, 5 nm, 32 chs, start wavelength: 500 nm to 510 nm
Condition 3 Pixel dwell = 4 s, Field of view = 0 m, Pixel size = 256 x 256, Pinhole = L
Requirement
5% or less in comparisons with the spectral graphs of the start wavelength 500 nm and
510 nm in all zones

5. Assembly check Check
5-1 Power supply Before At setup
The Ar laser power supply and confocal unit power supply are plugged to different outlets.
The total capacity is 40A (20A by 2) or greater.
5-2 Scan head Before At setup
The connection cables are correctly connected and firmly secured with the screws.
The FC connector of the excitation light fiber is correctly connected inserted to the end.
The ends of the excitation light and fluorescence fibers are clean.
The screws of the microscope securing mount are securely tightened.
Suitable 1st dichroic mirror is inserted correctly.
5-3 Controller Before At setup
The cable connections to the additional boards in the controller are secured.
The screws on the controller cover are correctly tightened.
The cables and wires of the controller are correctly connected.
5-4 Detector (including spectral detector) Before At setup
The cables and wires are correctly connected.
The filter cubes are set at the correct position.
The screws securing the fiber protection parts are fully tightened.
5-5 Microscope Before At setup
Make sure that the screws for the accessories are securely tightened.
The laser safety labels are affixed to the specified locations.
All the objective mounting holes on the nosepiece are occupied.
All laser shutters close when the safety interlock device is activated.
The laser safety kit is correctly attached.
5-6 Objective Before At setup
It is firmly secured.
The adjustment ring is correctly positioned.
The tip of the objective is clean.
5-7 Laser fixing parts Before At setup
All the screws for parts fixing the laser unit are fully tightened.
The unit and laser head are set without any gap between them.
The fibers are not bent.
The supplied stickers are affixed appropriately for the laser wavelengths.
5-8 Laser rack Before At setup
The power supplies are secured at the specified locations.
The cable connections are correct and firmly plugged.
The laser ON/OFF signal is correctly sent.
5-9 Z motor Before At setup
The external motor is correctly and firmly mounted.
The cables, etc. are correctly connected.
The rotating direction of the speed knob on the controller is correctly set.
5-10 Diascopic detector Before At setup
The IN/OUT function has no problem.
The adapter is correctly connected with the microscope.
The lamp house is correctly connected with the diascopic detector.
Fiber and cable connections for the diascopic detector are secure.

259
6. Settings check Check
6-1 Configuration Before At setup

The laser wavelength and position settings are correct.
The detector position is correctly set.
6-2 Objective Before At setup
The objectives are correctly registered.
6-3 Z-stack Before At setup
The displayed value is the same as that of the Z motor controller.
6-4 Orientation of images (when the inverted microscope is used) Before At setup

The top and bottom of visual images are the same as those of images on the screen.
(ConfigureC1-Mirrors-mirror image-x/y direction is checked when the inverted
microscope is used.)

6-5 Display setting Before At setup

The resolution is set to 1280 x 1024 or higher.
The screen saver is turned off.
The automatic sleep setting is off on the power supply control settings.
6-6 Saving the setting file Before At setup

The XML file is backed up.

Remarks:

Signature: Date:
Person in charge (User):
Setup engineer:

260

Table 18.0-1
Problem Possible cause Remedy
Unable to power on
system equipment
The controllers power cable is not
connected to the wall outlet.
Connect the power cable to the wall outlet.
The controllers power switch has not been
switched on.
Switch on the power switch.
The PC does not
function properly.
A system error has occurred. Restart the PC.
Unable to control the
system from the PC
There is a problem with the system
cabling.
See Chapter 15, Cable Connections between
Units and properly connect all cables.
The PC is unable to communicate with the
controller.
Turn off, and then turn on power to the controller.
The operation software NIS-Elements C
is not running properly.
Exit the operation software NIS-Elements C, and
restart it.
Unable to get images
The laser is off. Turn the laser on.
The laser shutter is closed.
Check the optical path settings with the operation
software NIS-Elements C.
The optical path selector lever on the
eyepiece tube is not set to the vertical tube
100% (upright microscope only).
Set the lever so that 100% of the light is guided into
the vertical tube.
The interlock mechanism is activated. Deactivate the interlock mechanism.
Improper setting has been made in 1st
dichroic mirror and filter cubes (2nd and
3rd dichroic mirrors).
Make filter setting appropriate for the excitation
wavelength and the fluorescent reagent used.
The specimen is not in focus. Bring it into focus again.
The incorrect channel is selected.
Check the optical path settings with the operation
software NIS-Elements C.
The laser output is too low.
Increase the laser power in the Acquisition screen
of the operation software NIS-Elements C.
The pinhole size is incorrect.
Increase the pinhole size in the Acquisition screen
of the operation software NIS-Elements C.
The applied voltage level is too low.
Increase the applied voltage (HV) in the
Acquisition screen of the operation software
NIS-Elements C.
The excessive light intensity warning
indicator on the operation window is
flashing.
Reset the applied voltage (HV) in the Acquisition
screen of the operation software NIS-Elements C.
The epi-fl filter cube is attached.
Remove the epi-fl filter cube from the confocal
microscopy position.
18 Troubleshooting
18 Troubleshooting
261
Problem Possible cause Remedy
The image is affected
by excessive noise.
The applied voltage level is too high.
Decrease the applied voltage (HV) in the
Acquisition screen of the operation software
NIS-Elements C.
Reduce the scan speed, increase the pinhole size,
or average the image if the image is too dark.
The optical fiber for excitation light is
vibrating.
Do not touch the optical fiber during image capture.
The image brightness
is too uneven.
The specimen is tilted relative to the optical
axis.
Place the specimen on the stage so that its surface
is level on the stage surface.
The repeatability of the
spectrum measurement
is poor.
The calibrated data is not read correctly.
Exit the operation software NIS-Elements C, and
restart it.
The measurement was not performed
under the specified conditions.
Perform the measurement under the conditions
described in Chapter 19, Specification.
The wavelength of the excitation laser is
not set correctly.
Correct the wavelength value in the XML file.
The light energy of the excitation laser is
too high.
Reduce the light energy of the excitation laser.
Ambient light enters into the system.
Turn off the ambient light source such as room
lightings and the lamphouse for dia-illumination.

262

C-LU3EX 3 laser unit
(MHF42305)
Number of lasers: 3
Laser wavelength: 1) Any one wavelength of the 405 nm, 440 nm, 594 nm, 633 nm,
and 638 nm
2) Either 488 nm or 514 nm, used fixedly or switchable by
the AREX EX changer
3) Any one wavelength of the 543 nm or 561 nm, (or 594 nm)
Light control: 0 to 100%
11 levels (manual)
With 0.1% steps (when optional AOM is used)
Shutter: For each laser beam
LU4A 4 laser unit A
(MHF41402)
Number of lasers: 4
Laser wavelength: 1) Any one wavelength of the 405 nm or 440 nm
2) 457 nm, 477 nm, 488 nm, or 514 nm
3) Any one wavelength of the 543 nm or 561 nm, or 594 nm
4) 638 nm
Light control: 0 to 100%
With 0.1% steps (AOTF)
Shutter: For each laser beam
Laser option
Recommended lasers
See Table: 1.2-3: Recommended lasers in Chapter 1, System Configuration.
LU-LR 4 laser PS rack (MHF44000/MHF44001/MHF44002)
Mountable lasers: 4 (Melles Griot products only)
Model: For 100 VAC area/for 120 VAC area/for 230 VAC area
C2-SH/C2-SHS
scan head
(MHA43200/MHA43300)
Laser inlet port: 1 port, FC connector
Fluorescence outlet port:
C2 scan head: 1 port, SMA connector
C2si scan head: 2 ports, FC and SMA connectors, switched manually
Pinhole: 6 types (S1, S2, M, M2, L, and O) with motorized switches
Scanner: Galvano scanner x 2
1st dichroic mirror: 10 types, switched manually
(For details, see Table 1.2-1: System overview in Chapter 1, System Configuration.)
Mount: ISO mount
C2-DU3 detector unit T
(MHE41400)
Number of channel: 3
Detector: PMT
Wavelength range: 400 nm to 750 nm
Mountable filter cube: x 2, switched manually
Filter cube: 17 types
(For details, see Table: 1.2-1: System overview in Chapter 1, System Configuration.)
PMT protection: Equipped
C2-DUT
diascopic detector unit
(MHE47300)
Number of channel: 1
Detector: PMT
DIC microscopy is available.
19 Specifications
19 Specifications
263
C2-DUS
spectral detector unit
(MHE43000)
Number of channels: 32
Detector: PMT
Wavelength resolution pitch: 3 steps (2.5 nm, 5 nm, and 10 nm)
Wavelength range acquired in batch: 77.5 nm (with 2.5 nm pitch), 155 nm (with 5 nm pitch),
310 nm (with 10 nm pitch)
Measurement wavelength: 420 nm to 750 nm
Excitation light mask: x 1, Automatic
Number of wavelengths for simultaneous excitation: 2
Virtual filter image acquisition: 4 ch (maximum)
Controller
(MHA43200/MHA43300)
Fuse ratings: 250 VAC, 6.3 A
5.2 mm x 20 mm
Electric shock protection class: Class 1
External trigger signal output:
Output: 1
Output voltage level: 5V TTL (L:0.5 V or less, H: 2.4 V or higher)
Output current: 20 mA (maximum)
Output pulse width: One-way horizontal scanning time
(32 sec or more. It depends on the frame rate.)
Output logic: Switchable between positive/negative logic
Connector: BNC connector (Ext Trig Out)
Output timing: Start of imaging (Pulse output when scanning the first horizontal
line), output by frame
Images
< When C2-DU3 detector unit T is used >
Number of channels that can be acquired:
Fluorescence: 3
Transmitted: 1
Scale: 12 bits (4096 steps)
Pixel: (32), 64, 128, 256, 512, 1024, 2048
32 is allowed only for vertical pixels.
256 or 512 for bidirectional scanning
Scan area: Square inscribing the field number of 18 (12.7 x 12.7)
Zoom: Variable: 1x to 1000x
Speed: 1 frame/second (512 x 512, one-way scanning)
2 frames/second (512 x 512, bidirectional scanning)
3 frames/second (512 x 512, high-speed, bidirectional scanning)
< Ultrahigh-speed model (C2plus) >
8 frames/second (512 x 512, ultrahigh-speed, bidirectional
scanning)
100 frames/second (512 x 32, ultrahigh-speed, bidirectional
scanning)
8x zoom or more equipped
Rotation scanning, ROI scanning and CROP unavailable
Scan mode: 2-dimensional: X-T, Y-T, X-Y
3-dimensional: X-Y-T, X-Y-Z
4-dimensional: X-Y-Z-T
Mirror image (X, Y)
Reciprocation image
Rotation image (-180 to +180, 1step)
ROI image (when AOM or AOTF is used)
Line sequential acquired (when 4 laser unit A is used)
19 Specifications
264
Images
< When C2-DUS spectral detector unit is used >
Number of channels that can be acquired:
Spectral: 32
Transmitted: 1
Scale: 12 bits (4096 steps)
Pixel: (32), 64, 128, 256, 512, 1024
32 is allowed only for vertical pixels.
Scan area: Square inscribing the field number of 18 (12.7 x 12.7)
Zoom: 1x to 1000x
Speed: 1/2 frame/second (512 x 512, one-way)
Scan mode: 2-dimensional: X-T, Y-T, X-Y
3-dimensional: X-Y-T, X-Y-Z
4-dimensional: X-Y-Z-T
Mirror image (X, Y)
Rotation image (-180 to +180, 1step)
ROI image (when AOM or AOTF is used)
Virtual filter image
Virtual filter line sequential acquired
Microscope
Upright microscopes:
80i/90i: With DIH, C1 TE mount, and A1-DIH interlock cable
With C-TT-C trinocular tube T-C or C1 Y-TT trinocular tube
Ni-E/U: With NI-TT quadrocular tilting tube or NI-TT-E motorized
quadrocular tilting tube, C2-NI-TT quadrocular tilting tube
Adapter, and A1-TII interlock cable
(When any of NI-FLT6 epi-fluorescence cube turret, NI-FLT6-E
motorized epi-fluorescence cube turret, or NI-FLT6-I intelligent
epi-fluorescence cube turret is used, NI-AST analyzer slider
stopper is necessary.)
With C-TT-C trinocular tube T-C
FN1: With NI-TT quadrocular tilting tube, C2-NI-TT quadrocular tilting
tube adapter, and A1-TII interlock cable
(When NI-FLT6 epi-fluorescence cube turret is used, NI-AST
analyzer slider stopper is necessary.)
With C-TT-C trinocular tube T-C or C1 Y-TT trinocular tube
AZ100: With AZ-TE100LS ergonomic trinocular tube 100LS (with
interlock cable extending out) and AZ 100 stage cover
Inverted microscope
Ti-E/U: With Ti laser safety kit, C1-TI Ti mounting adapter and A1-TII
interlock cable
Motorized focus
(optional)
80i: Z drive 99888 (manufactured by Conix)
Adapter 99881 (manufactured by Conix)
Ni-U: Note: Ask Nikon representatives for the model.
Model TBD (manufactured by Prior)
FN1: Remote focus kit NIKRFK (manufactured by Prior)
Remote focus adapter kit NIKFN1MA (manufactured by Prior)
Optional encoder kit NIKFN1EK (manufactured by Prior)
AZ100: Z drive 99885 (manufactured by Conix)
Adapter 99885 (manufactured by Conix)
Ti-U: Remote focus kit NIKRFK (manufactured by Prior)
Remote focus adapter kit NIKTIMA (manufactured by Prior)
19 Specifications
265
PC
Recommended model: Z400 (manufactured by HP)
CPU: Intex Xeon W3565 (3.20 GHz/8 MB/1066 MHz)
RAM: DDR3 1333 4GB
Chipset: Intel
X58 Express chipset

HDD: Serial ATA 3 GB/s (7,200 rpm) 146 GB or higher
Graphics: NVIDIA Quadro FX 380, 256M (PCI Express/dual display)
Drive: Super Multi drive, up to x16 speed or higher
Expansion slots: PCI Express2.0 x16 slot (for graphics) x 1
PCI Express (x8 mechanical slot or x4 electrical slot) x 1
*1

PCI slot x 1
*2

Interface: USB 2.0
*3
, RS-232C
*1

LAN port: 10/100/1000 network interface x 1
Monitor: LCD2 monitor with 1600 x 1200 resolutions or higher
(recommended)
Keyboard, mouse: USB keyboard, USB mouse
OS: Windows 7 Professional 64 bit edition
D/A card: National Instruments made, PCI-6711, PCI-6713, or PCI-6733
*2

Driver software: NI-DAQmx Ver. 9.0.2 (Windows 7, 64 bits supported)
Software: NIS-Elements C (MHS50000)
*1: Required when connecting to the microscope with RS-232C (REX-PE60 (PCI Express x1)
manufactured by RATOC System, Inc. is recommended.)
*2: Required to control laser modulation with AOM/AOTF/LD
*3: Required when connecting to the microscope with USB
Power supply
Controller: 100 - 240 VAC, 50/60 Hz, 4A - 2A
4 laser unit A: 100 - 240 VAC, 50/60 Hz, 2A - 1A
AOM controller: 100 - 240VAC, 50/60 Hz, 1.2A - 0.6A
4 laser PS rack: Model for 100-VAC area 100 VAC, 50/60 Hz, 15A
100 VAC, 50/60 Hz, 3A
Model for 120-VAC area 115 VAC, 50/60 Hz, 15A
115 VAC, 50/60 Hz, 3A
Model for 230-VAC area 230 VAC, 50/60 Hz, 7.5A
230 VAC, 50/60 Hz, 1.5A
Weight
3 laser unit EX: 24 kg (without laser)
4 laser unit A: 43 kg (without laser)
4 laser PS rack: 20 kg (without laser power supply)
AOM controller: 4.5 kg
Scan head: 2 kg
Detector unit: 3.5 kg
Spectral detector: 23 kg
Controller: 12 kg
Environmental
conditions
Operating conditions: Temperature: +5 to +35C
+18 to +28C (when using 4 laser unit A or
under the spectrum measurement)
Humidity: 60%RH or lower (no condensation)
Altitude: 2000 m or lower
Pollution level: Degree 2
Overvoltage category: Category II
Short-term temporary overvoltage: 1,440V (up to 5 seconds)
Long-term temporary overvoltage: 490V (longer than 5 seconds)
Indoor use only
Storage conditions: Temperature: 0 to +35C
Transport conditions: Temperature: -20 to +50C
19 Specifications
266
Standards
EU
CE marking
Low Voltage Directive
EN61010-1:2001 (Safety requirements for electrical equipment for measurement,
control and laboratory use)
EN60825-1:2007 (Laser safety)
Laser Class 3B
EMC Directive
EN61326-1:2006 (EMC for electrical equipment for measurement, control and
laboratory use)
EN55011:2007+A2:2007 Class A (EMI)
EN61000-3-2:2006 (Harmonics)
EN61000-3-3:1995+A1:2001+A2:2005 (Flicker)
EN61000-4-2:2009 (Electro-static discharge)
EN61000-4-3:2006+A1:2008 (Radiated immunity)
EN61000-4-4:2004 (Electrical fast transients)
EN61000-4-5:2006 (Surge)
EN61000-4-6:2009 (Conducted immunity)
EN61000-4-8:1993+A1:2001 (Power frequency magnetic)
EN61000-4-11:2004 (Dips and sags)
USA
FDA
Notice 50 is applied.
CDRH 21CFR Part1040.10 (Laser products performance)
Laser Class IIIb
FCC
Subpart 15B ClassA (Unintentional Radiators)
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment.
This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in
which case the user will be required to correct the interference at his own expense.
This Class A digital apparatus complies with Canadian ICES-003(A) / NMB-003(A).
Cet appareil numrique de la classe A est conforme la norme NMB-003(A) du Canada.
Others
Canada ICES-003(A) / NMB-003(A)
Australia AS/NZS CISPR11:2002
Export Regulations
N.B. Export of the products* in this manual is controlled under the Japanese Foreign Exchange
and Foreign Trade Law. Appropriate export procedure, such as obtaining of export license, shall
be required in case of export from Japan.
* Products: Hardware and its technical information (including software)

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