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PENUNTUN PRAKTIKUM
Ilmu Dasar Keperawatan (IDK II)

Pewarnaan Bakteri

Kuntarti/IDK2/2011

Pewarnaan Bakteri
Topik : Pewarnaan Gram

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Tujuan Pewarnaan : a. Mengetahui bahwa bakteri dapat dilihat dengan pewarnaan b. Mengenal bakteri positif Gram dan negatif Gram serta morfologinya

Kuntarti/IDK2/2011

Faktor - faktor penentu keberhasilan Pewarnaan :

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1. Gelas alas bersih dan bebas lemak 2. Umur biakan (optimal) : 18 - 24 jam kecuali bakteri tahan asam (M. tuberculosis) 3. Kualitas zat warna 4. Tebal tipisnya sediaan

Hasil pewarnaan : bakteri Gram positif : ungu bakteri Gram negatif : merah
Kuntarti/IDK2/2011

Cara pembuatan sediaan

From liquid media
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From solid media

Pewarnaan Gram
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Gently stain with crystal violet For 1-2 minutes

Gently stain wash off the stain With tap water

Gently apply Grams iodine for 45 60 seconds

Counterstain with Safranin for 45 secons until 1 minutes

Gently wash off the Safranin with tap water

Kuntarti/IDK2/2011

Staphylococcus sp. Vibrio sp.

PERTUNJUKAN :

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Kuntarti/IDK2/2011

Escherichia coli
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B. subtilis

Kuntarti/IDK2/2011

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PEWARNAAN BAKTERI TAHAN ASAM

Tujuan :
1. Mengetahui teknik pewarnaan bakteri tahan asam 2. Mampu mengidentifikasi bakteri tahan asam secara mikroskopik Pewarnaan yang digunakan : a. Kinyoun Gabbet (Tan Thiam Hok) b. Ziehl Neelsen (WHO)
Kuntarti/IDK2/2011

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Prinsip :
a. Fukhsin karbol ikat (cuci dengan asam alkohol); tahan asam (merah) b. Fukhsin karbol lepas (cuci dengan asam alkohol); bukan tahan asam (biru)

Kuntarti/IDK2/2011

Pewarnaan Tahan Asam

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Kuntarti/IDK2/2011

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Biakan M. tuberculosis pada agar Lowenstein Jensen

M. tuberculosis dengan pewarnaan Ziehl Nelsen

Kuntarti/IDK2/2011

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Kuntarti/IDK2/2011

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