Brandon

Radu
Science 6

REGENERATION LAB

PROBLEM: Which part of the planarian will regenerate first when trisected, anterior,
posterior or mid section?

HYPOTHESIS: If trisected, then the mid section will regenerate first.

THEORY:

Planaria are flatworms that reproduce sexually. The planaria are
hermaphrodites which means that they consist of both Male and female gonads. In
sexual reproduction, two planarians meet, exchanged sperm through the genital
pores, When Gametes (which are matured haploid germ cells) from each planaria
combine sperm is and received by the other planaria. An egg forms with a new
planaria in it.

When conditions are less the ideal, planaria are able to reproduce asexually.
For asexual reproduction, the planaria detaches his tale end and each half regrows
the lost parts by regeneration. When both half of the body regrows then you have
two planaria, then the two planaria reproduce and make another planaria.

Planaria can reproduce asexually through the process of regeneration.
Regeneration is when a planaria can be cut up into pieces, and each piece can
regrow into a complete organism. The reason why I thought that the mid section
would regenerate first was because the mid section can grow the Anterior or the
posterior at the same time when the Anterior would have to grow the mid section
before the Posterior and the Posterior would have to also grow the mid section first.


Data

Radu, Brandon

Friday, May 23, 2014 1:49:28 PM Pacific Daylight Time

70:56:81:af:d8:f1

First Regenerated %

2014 Regeneration Data

100
90
80
70
60
50
40
30
20
10
0

43

50 49

6th period

37

7th Grade
7

Anterior

Mid Section

14

Posterior

CONCLUSION:


In this lab we trisected a planaria to see which of the three parts will
regenerate first. I hypothesized that the mid section would regenerate first.
My data shows by day 7 the Anterior had regenerated the most cells. On day 7
the Anterior had completely regenerated mid section and started to form the
Posterior. In the 7th grade 37% of Anterior cells grow back first, 49% of the
Mid Section grew back first, and only 14% of the Posterior sections grow back
first. In my class (6th period) we had similar and different percentages. 43% of
the Anterior grew back first, 50% of the mid section grew back first, and only
7% of the Posterior grew back first. There was a 6% difference for the
Anterior, a 1% difference for Mid Section, and 7% difference With the
Posterior. Between my class and the whole seventh grade I think the data
ended up pretty similar. In conclusion, my hypothesis was correct. The
planaria will regenerate when trisected and the mid section is likely to
regenerate first.




ANALYSIS

Radu, Brandon

Friday, May 23, 2014 1:49:28 PM Pacific Daylight Time

70:56:81:af:d8:f1

 In this lab, the data shows that my results were valid. If you look at the
graph you would see that my classes results were very similar to the whole
entire grades results. In every single class about 50% had Mid Sections that
regenerated first, 35% of the Anterior Regenerated first, and about 10%
percent of the Posterior regenerating first. I order to make my results more
reliable I would, assign more then one planaria to each group so then we
would have two times they data to compare to.

Planaria stem cells are different from human stem cells. Twenty
percent of planaria stem cells are totipotent throughout their lifetime. While
in embryonic form, humans are mostly comprised of pluripotent or embryonic
stem cells. This changes as humans develop. By adulthood, stem cells (now call
somatic cells) are only found in small pockets around the body in bone
marrow, teeth, blood, blood vessels, gut, heart and liver. Embryotic stem cells
are pluripotent when planaria stem cells are Totipotent. The difference
between the two is totipotent stem cells can give rise to more types of cells
then pluripotent stem cell
The controversy over stem cell research is less than what it used to be.
This situation is due to two new ways of scientist obtaining stem cells, both IPS
and STAP. So now HES is no longer the only way to obtain stem cells. IPS, or
Induced Pluripotent stem cells, is a way of making fake pluripotent stem cells,
by adding genes to a differentiated cell to undifferentiated it. STAP, or
stimulus-triggered acquisition of pluripotency, is another way of creating fake
stem cells through a differentiated cell until it reforms back into its original
un-differentiated form. Although these two methods have proved that they
work, scientists are still not sure of the effects that the STAP therapy can
provide. HES is another method for obtaining pluripotent embryonic stem
cells. The reason there was and still is a little controversy over HES is because
in order to obtain embryonic stem cells, an embryo must be destroyed. Even
though the embryo is usually six weeks old.

Radu, Brandon

Friday, May 23, 2014 1:49:28 PM Pacific Daylight Time

70:56:81:af:d8:f1