Test Required Method Details of Test Comments Gateway PCR All plants tested for presence of unintended events (including RT200) 900 seeds (<0.5% AP @ 99% conf.) PCR Testing for presence of unintended events (including RT200) Only required if generation wasn't gateway'd Trait Testing Roll towel; Spray trays; RuR test strips; PCR To confirm presence of Roundup Ready Trait in line max 3% impurity level @ 95% confidence level 600 seeds (<0.5% AP @ 95% conf.) PCR Testing for presence of unintended events (including RT200) Trait Testing Roll towel; Spray trays; RuR test strips; PCR To confirm presence of Roundup Ready Trait in line max 3% impurity level @ 95% confidence level 1400 seeds PCR Testing for presence of unintended events (not specific for RT200) Inhouse testing requirement only (not required by Monsanto) Trait Testing Roll towel; Spray trays; RuR test strips; PCR To confirm presence of Roundup Ready Trait in line max 15% impurity level @ 95% confidence level Gateway PCR All plants tested for presence of unintended events 900 seeds (<0.5% AP @ 99% conf.) PCR Testing for presence of unintended events Only required if generation wasn't gateway'd Basic nil required Testing required only if contamination expected *Monsanto requirement for unintended events includes RT200 only, internal requirements require additional screening as appropriate REPORTING DATES MAIN SEASON PRODUCTION CONTRA PRODUCTION STAGE 1 August 1 March 1 STAGE 2 August 1 March 1 STAGE 3 August 1 March 1 STAGE 4 March 1 (prior to planting) March 1 (prior to planting) R O U N D U P
R E A D Y C O N V E N T I O N A L Production Stage Prebasic Basic Commercial Prebasic QCLM-4 Genetic Purity Testing Copyright Pacific Seeds Pty Ltd Last updated March 2014 S:\qc\GENETIC PURITY\CANOLA\ROUNDUP READY\ Roundup Ready - Testing Summary.xls