Materials & Streams Report

May 6, 2014

for LAB 4
1. OVERALL PROCESS DATA
Annual Operating Time
Recipe Batch Time
Recipe Cycle Time
Number of Batches per Year
MP = Undefined

7,891.65h
132.96h
34.48h
226.00

- Page 1 -

2.1 STARTING MATERIAL REQUIREMENTS (per Section)

Section

Starting Material Active Product

Amount
Needed
(kg
Sin/kg
MP)

Main Section

(none)

Unknown Unknown Unknown Unknown

(none)

Molar
Yield
(%)

Mass
Yield
(%)

Gross
Mass
Yield
(%)

Sin = Section Starting Material, Aout = Section Active Product

2.2 BULK MATERIALS (Entire Process)

Material
Process Water
Glucose
Salts
H3PO4 (5% w/w)
NaOH (0.5 M)
WFI
Air
Ammonia
NaCI (0.1 M)
NaCI (0.5 M)
Tris Buffer
TOTAL

kg/yr

kg/batch

17,139,602
1,388,318
231,876
2,394,257
24,301,640
49,879,919
13,185,913
89,270
30,019,406
2,552,469
38,686,092
179,868,763

75,838.947
6,143.001
1,025.999
10,594.057
107,529.380
220,707.608
58,344.747
395.000
132,829.232
11,294.110
171,177.400
795,879.481

kg/kg MP

- Page 2 -

2.3 BULK MATERIALS (per Section)

SECTIONS IN: Main Branch
Main Section
Material

kg/yr

kg/batch

17,139,602
1,388,318
231,876
2,394,257
24,301,640
49,879,919
13,185,913
89,270
30,019,406
2,552,469
38,686,092
179,868,763

75,838.947
6,143.001
1,025.999
10,594.057
107,529.380
220,707.608
58,344.747
395.000
132,829.232
11,294.110
171,177.400
795,879.481

% Total

kg/yr

kg/batch

86.25
1.93
1.86
1.84
1.47
1.84
1.44
0.76
0.78
1.84
100.00

14,782,085
331,247
318,191
314,724
251,912
314,724
247,161
130,429
134,404
314,724
17,139,602

65,407.457
1,465.693
1,407.925
1,392.586
1,114.655
1,392.586
1,093.631
577.120
594.708
1,392.586
75,838.947

% Total

kg/yr

kg/batch

100.00
100.00

1,388,318
1,388,318

6,143.001
6,143.001

Process Water
Glucose
Salts
H3PO4 (5% w/w)
NaOH (0.5 M)
WFI
Air
Ammonia
NaCI (0.1 M)
NaCI (0.5 M)
Tris Buffer
TOTAL

kg/kg MP

2.4 BULK MATERIALS (per Material)

Process Water
Procedure
Main Section (Main Branch)
P-1
P-5
P-7
P-9
P-10
P-11
P-13
P-16
P-19
P-21
TOTAL

Glucose
Procedure
Main Section (Main Branch)
P-1
TOTAL

kg/kg MP

kg/kg MP

- Page 3 -

Salts
Procedure
Main Section (Main Branch)
P-1
TOTAL

H3PO4 (5% w/w)

Procedure
Main Section (Main Branch)
P-1
P-5
P-7
P-9
P-10
P-11
P-13
P-16
P-19
P-21
TOTAL

NaOH (0.5 M)
Procedure
Main Section (Main Branch)
P-1
P-5
P-7
P-9
P-10
P-11
P-13
P-16
P-19
P-21
P-15
TOTAL

WFI
Procedure
Main Section (Main Branch)
P-1
P-5
P-7
P-9
P-10
P-11
P-13
P-16
P-18
P-19
P-20

% Total

kg/yr

kg/batch

100.00
100.00

231,876
231,876

1,025.999
1,025.999

% Total

kg/yr

kg/batch

11.89
12.38
11.89
11.76
9.42
11.76
9.24
4.87
5.02
11.76
100.00

284,638
296,415
284,733
281,631
225,423
281,631
221,171
116,714
120,271
281,631
2,394,257

1,259.460
1,311.572
1,259.878
1,246.153
997.447
1,246.153
978.633
516.435
532.174
1,246.153
10,594.057

% Total

kg/yr

kg/batch

10.08
2.10
2.02
2.00
1.60
2.00
1.57
0.83
0.85
2.00
74.96
100.00

2,448,705
510,005
489,904
486,874
387,857
486,874
380,542
200,816
206,936
486,874
18,216,253
24,301,640

10,834.979
2,256.659
2,167.716
2,154.310
1,716.182
2,154.310
1,683.813
888.565
915.645
2,154.310
80,602.891
107,529.380

% Total

kg/yr

kg/batch

1.28
1.33
1.28
1.26
1.01
1.26
0.99
0.52
31.39
0.54
6.00

636,170
662,493
636,382
629,449
503,824
629,449
494,321
260,858
15,656,763
268,808
2,993,591

2,814.914
2,931.386
2,815.849
2,785.172
2,229.310
2,785.172
2,187.262
1,154.240
69,277.710
1,189.417
13,245.980

kg/kg MP

kg/kg MP

kg/kg MP

kg/kg MP

- Page 4 -

P-21
P-15
TOTAL

Air
Procedure
Main Section (Main Branch)
P-3
TOTAL

Ammonia
Procedure
Main Section (Main Branch)
P-5
TOTAL

NaCI (0.1 M)
Procedure
Main Section (Main Branch)
P-18
P-15
TOTAL

NaCI (0.5 M)
Procedure
Main Section (Main Branch)
P-15
TOTAL

Tris Buffer
Procedure
Main Section (Main Branch)
P-15
TOTAL

1.26
51.88
100.00

629,449
25,878,361
49,879,919

2,785.172
114,506.024
220,707.608

% Total

kg/yr

kg/batch

100.00
100.00

13,185,913
13,185,913

58,344.747
58,344.747

% Total

kg/yr

kg/batch

100.00
100.00

89,270
89,270

395.000
395.000

% Total

kg/yr

kg/batch

52.44
47.56
100.00

15,743,277
14,276,129
30,019,406

69,660.517
63,168.714
132,829.232

% Total

kg/yr

kg/batch

100.00
100.00

2,552,469
2,552,469

11,294.110
11,294.110

% Total

kg/yr

kg/batch

100.00
100.00

38,686,092
38,686,092

171,177.400
171,177.400

kg/kg MP

kg/kg MP

kg/kg MP

kg/kg MP

kg/kg MP

- Page 5 -

2.5 BULK MATERIALS: SECTION TOTALS (kg/batch)

Raw Material
Process Water
Glucose
Salts
H3PO4 (5% w/w)
NaOH (0.5 M)
WFI
Air
Ammonia
NaCI (0.1 M)
NaCI (0.5 M)
Tris Buffer
TOTAL

Main Section
75,838.947
6,143.001
1,025.999
10,594.057
107,529.380
220,707.608
58,344.747
395.000
132,829.232
11,294.110
171,177.400
795,879.481

2.6 BULK MATERIALS: SECTION TOTALS (kg/yr)

Raw Material
Process Water
Glucose
Salts
H3PO4 (5% w/w)
NaOH (0.5 M)
WFI
Air
Ammonia
NaCI (0.1 M)
NaCI (0.5 M)
Tris Buffer
TOTAL

Main Section
17,139,602
1,388,318
231,876
2,394,257
24,301,640
49,879,919
13,185,913
89,270
30,019,406
2,552,469
38,686,092
179,868,763

- Page 6 -

3. STREAM DETAILS
Stream Name
Source
Destination
Stream Properties

Water
INPUT
P-1

Nutrients
INPUT
P-1

S-104
P-1
P-2

S-107
P-2
P-5

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

0.00
25.00
1.01
994.70
0.00
0.00
1.00

0.00
25.00
1.01
1,144.20
0.00
0.00
0.55

0.00
25.00
10.12
1,007.97
0.00
0.00
0.95

0.00
35.00
10.12
1,004.09
788.29
9.53
0.95

Glucose
Process Water
Salts
TOTAL (kg/batch)
TOTAL (L/batch)

0.000
64,000.000
0.000
64,000.000
64,340.728

6,143.001
0.000
1,025.999
7,169.000
6,265.520

6,143.001
64,000.000
1,025.999
71,169.000
70,606.248

6,143.001
64,000.000
1,025.999
71,169.000
70,879.371

Stream Name
Source
Destination
Stream Properties

Ammonia
INPUT
P-5

S-108
P-4
P-5

S-110
P-5
P-6

S-111
P-5
P-7

0.00
25.00
1.01
0.70
131.08
285.52
0.50

0.00
40.00
6.01
6.66
246.29
3.63
0.24

0.00
20.00
1.01
1.22
78.41
1.13
0.24

0.00
37.00
1.01
992.65
975.86
11.97
1.00

395.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
395.000
567,455.178

0.000
0.000
0.000
0.000
44,757.299
13,587.449
0.000
0.000
58,344.747
8,756,514.853

39.798
0.000
4,489.470
0.000
44,821.912
10,483.693
0.000
0.000
59,834.872
48,879,929.581

0.000
2,566.587
0.000
122.860
0.000
0.000
67,284.990
183.179
70,157.616
70,676.770

Component Flowrates (kg/batch)

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Component Flowrates (kg/batch)

Ammonia
Biomass
Carb. Dioxide
Glucose
Nitrogen
Oxygen
Process Water
Salts
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 7 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Air
INPUT
P-3

S-106
P-3
P-4

Gas Out
P-6
OUTPUT

S-112
P-8
P-7

0.00
25.00
1.01
1.18
0.00
0.00
0.24

0.00
40.00
6.01
6.66
246.29
3.63
0.24

0.00
20.00
1.01
1.22
78.41
1.13
0.24

0.00
25.00
1.01
998.80
0.00
0.00
1.00

0.000
0.000
0.000
0.000
44,757.299
13,587.449
0.000
0.000
58,344.747
49,477,338.950

0.000
0.000
0.000
0.000
44,757.299
13,587.449
0.000
0.000
58,344.747
8,756,514.853

39.798
0.000
4,489.470
0.000
44,821.912
10,483.693
0.000
0.000
59,834.872
48,879,929.581

0.000
2,566.587
0.000
59.229
0.000
0.000
32,437.279
88.308
35,151.404
35,193.584

S-115
P-7
OUTPUT

S-116
P-7
P-9

S-113
P-7
P-8

Liquid Waste-1
P-8
OUTPUT

0.00
33.26
1.01
1.15
0.28
2.00
0.24

0.00
25.01
1.01
998.80
0.30
0.01
1.00

0.00
37.00
1.01
992.66
975.55
11.96
1.00

0.00
25.00
1.01
994.98
0.00
0.00
1.00

0.000
0.000
93.299
28.324
0.000
0.000
121.622
105,994.648

2,566.587
59.229
0.000
0.000
32,437.279
88.308
35,151.404
35,193.672

2,566.587
122.860
0.000
0.000
67,284.990
183.179
70,157.616
70,676.673

0.000
63.631
0.000
0.000
34,847.711
94.871
35,006.212
35,182.827

Component Flowrates (kg/batch)

Ammonia
Biomass
Carb. Dioxide
Glucose
Nitrogen
Oxygen
Process Water
Salts
TOTAL (kg/batch)
TOTAL (L/batch)

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Component Flowrates (kg/batch)

Biomass
Glucose
Nitrogen
Oxygen
Process Water
Salts
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 8 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

S-118
P-9
P-10

S-119
P-10
OUTPUT

S-120
P-10
P-11

S-121
P-11
P-12

0.00
15.00
1.01
1,002.27
- 395.70
- 9.69
0.97

0.00
15.00
1.01
1.22
- 0.12
- 2.42
0.24

0.00
15.00
1.01
1,002.27
- 395.57
- 9.68
0.97

0.00
15.00
1.01
1,002.11
- 389.90
- 9.69
0.97

121.128
13.496
765.927
59.229
0.000
510.618
0.000
32,437.279
1,155.418
88.308
35,151.404
35,071.859

0.000
0.000
0.000
0.000
31.604
0.000
9.594
0.000
0.000
0.000
41.198
33,765.306

121.128
13.496
765.927
59.229
0.000
510.618
0.000
32,437.279
1,155.418
88.308
35,151.404
35,071.899

119.587
0.270
703.121
58.476
0.000
504.122
0.000
32,024.590
1,140.718
87.185
34,638.069
34,564.981

Component Flowrates (kg/batch)

B-Gal
Biomass
Debris
Glucose
Nitrogen
Nucleic Acids
Oxygen
Process Water
Proteins
Salts
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 9 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Debris-1
P-11
OUTPUT

S-123
P-12
P-13

Debris-2
P-12
OUTPUT

S-102
P-14
P-13

0.00
15.00
1.01
1,012.74
- 5.80
- 9.73
0.97

0.00
15.00
1.01
1,000.23
- 372.99
- 9.69
0.97

0.00
15.00
1.01
1,045.55
- 16.90
- 9.66
0.97

0.00
15.00
9.82
1,001.42
- 182.61
- 9.47
0.95

1.541
13.226
62.806
0.754
6.496
412.689
14.700
1.124
513.335
506.878

118.391
0.000
0.000
57.168
478.916
31,308.610
1,083.682
85.236
33,132.002
33,124.533

1.196
0.270
703.121
1.307
25.206
715.981
57.036
1.949
1,506.066
1,440.448

114.839
0.000
0.000
27.795
232.849
15,222.280
946.492
41.442
16,585.698
16,562.180

S-101
P-13
P-15

S-126
P-13
P-14

Liquid Waste-2
P-14
OUTPUT

WFI-2
INPUT
P-15

0.00
15.00
1.01
1,001.42
- 182.61
- 9.47
0.95

0.00
15.00
9.82
1,000.22
- 372.87
- 9.68
0.97

0.00
15.00
9.82
999.04
- 190.35
- 9.90
0.99

0.00
25.00
1.01
994.70
0.00
0.00
1.00

114.839
27.795
232.849
15,222.280
946.492
41.442
0.000
16,585.698
16,562.180

118.391
57.168
478.916
31,308.610
1,083.682
85.236
0.000
33,132.002
33,124.572

0.000
29.373
246.066
16,086.330
140.742
43.794
0.000
16,546.305
16,562.179

0.000
0.000
0.000
0.000
0.000
0.000
94,232.373
94,232.373
94,734.054

Component Flowrates (kg/batch)

B-Gal
Biomass
Debris
Glucose
Nucleic Acids
Process Water
Proteins
Salts
TOTAL (kg/batch)
TOTAL (L/batch)

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Component Flowrates (kg/batch)

B-Gal
Glucose
Nucleic Acids
Process Water
Proteins
Salts
WFI
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 10 -

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Tris
INPUT
P-15

NaCl-1
INPUT
P-15

NaOH
INPUT
P-15

WFI-3
INPUT
P-15

0.00
25.00
1.01
1,084.16
0.00
0.00
0.93

0.00
25.00
1.01
1,000.20
0.00
0.00
1.00

0.00
25.00
1.01
1,021.00
0.00
0.00
0.99

0.00
25.00
1.01
994.70
0.00
0.00
1.00

0.000
0.000
13,095.071
158,082.329
171,177.400
157,890.089

369.221
0.000
0.000
62,799.493
63,168.714
63,156.036

0.000
1,579.817
0.000
79,023.074
80,602.891
78,945.045

0.000
0.000
0.000
20,273.651
20,273.651
20,381.586

NaCl-2
INPUT
P-15

S-125
P-15
P-16

S-105
P-15
OUTPUT

S-127
P-17
P-16

0.00
25.00
1.01
1,008.72
0.00
0.00
0.99

0.00
24.78
1.01
999.93
- 1.25
- 0.22
0.99

0.00
24.64
1.01
1,013.19
- 181.36
- 0.34
0.97

0.00
25.19
1.01
1,000.29
0.35
0.19
0.98

0.000
0.000
0.000
0.000
0.000
0.000
323.012
0.000
0.000
10,971.098
11,294.110
11,196.432

103.355
0.000
0.582
0.000
28.395
0.000
48.452
0.000
0.000
4,686.712
4,867.497
4,867.842

11.484
27.795
232.267
15,222.280
918.097
41.442
643.781
1,579.817
13,095.071
420,695.306
452,467.340
446,575.516

98.188
0.000
0.183
0.000
33.563
0.000
15.260
0.000
0.000
1,476.049
1,623.242
1,622.764

Component Flowrates (kg/batch)

Sodium Chloride
Sodium Hydroxid
TRIS HCl
WFI
TOTAL (kg/batch)
TOTAL (L/batch)

Stream Name
Source
Destination
Stream Properties
Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Component Flowrates (kg/batch)

B-Gal
Glucose
Nucleic Acids
Process Water
Proteins
Salts
Sodium Chloride
Sodium Hydroxid
TRIS HCl
WFI
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 11 -

Stream Name
Source
Destination
Stream Properties

S-129
P-16
OUTPUT

S-130
P-16
P-18

S-131
P-16
P-17

Liquid Waste-4
P-17
OUTPUT

0.00
24.78
1.01
1.18
- 0.00
- 0.05
0.24

0.00
25.19
0.14
1,000.29
0.35
0.19
0.98

0.00
24.78
1.01
999.93
- 1.25
- 0.22
0.99

0.00
25.19
1.01
999.60
0.72
0.19
0.99

0.000
4.403
0.000
1.337
0.000
0.000
0.000
5.740
4,863.805

98.188
0.000
0.183
0.000
33.563
15.260
1,476.049
1,623.242
1,622.764

103.355
0.000
0.582
0.000
28.395
48.452
4,686.712
4,867.497
4,867.842

0.000
0.000
0.399
0.000
0.000
33.192
3,210.663
3,244.254
3,245.538

Stream Name
Source
Destination
Stream Properties

S-134
INPUT
P-18

S-133
INPUT
P-18

S-135
P-18
P-19

Liquid Waste-5
P-18
OUTPUT

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

0.00
25.00
1.01
994.70
0.00
0.00
1.00

0.00
25.00
1.01
1,000.20
0.00
0.00
1.00

0.00
25.00
0.14
997.49
0.02
0.00
1.00

0.00
25.00
0.14
996.12
0.33
0.00
1.00

0.000
0.000
0.000
0.000
69,277.710
69,277.710
69,646.536

0.000
0.000
0.000
407.166
69,253.352
69,660.517
69,646.536

88.369
0.000
0.336
30.537
5,194.001
5,313.243
5,326.600

9.819
0.183
33.227
391.888
134,813.110
135,248.227
135,775.162

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

Component Flowrates (kg/batch)

B-Gal
Nitrogen
Nucleic Acids
Oxygen
Proteins
Sodium Chloride
WFI
TOTAL (kg/batch)
TOTAL (L/batch)

Component Flowrates (kg/batch)

B-Gal
Nucleic Acids
Proteins
Sodium Chloride
WFI
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 12 -

Stream Name
Source
Destination
Stream Properties

S-141
P-20
P-19

S-137
P-19
OUTPUT

S-138
P-19
P-21

S-139
P-19
P-20

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

0.00
25.00
1.01
994.72
0.00
0.00
1.00

0.00
25.00
1.01
1.18
0.00
0.00
0.24

0.00
25.00
1.01
994.72
0.00
0.00
1.00

0.00
25.00
1.01
997.49
0.02
0.00
1.00

85.718
0.000
0.000
0.001
0.101
0.009
2,563.416
2,649.245
2,663.300

0.000
7.228
2.194
0.000
0.000
0.000
0.000
9.422
7,989.900

85.718
0.000
0.000
0.001
0.101
0.009
2,563.416
2,649.245
2,663.300

88.369
0.000
0.000
0.336
30.537
0.000
5,194.001
5,313.243
5,326.600

Stream Name
Source
Destination
Stream Properties

S-140
INPUT
P-20

Liquid Waste-6
P-20
OUTPUT

S-143
P-21
OUTPUT

S-144
P-21
OUTPUT

Activity (U/ml)
Temperature (C)
Pressure (bar)
Density (g/L)
Total Enthalpy (kW-h)
Specific Enthalpy (kcal/kg)
Heat Capacity (kcal/kg-C)

0.00
25.00
1.01
994.70
0.00
0.00
1.00

0.00
25.00
1.01
995.63
0.00
0.00
1.00

0.00
12.00
1.01
999.44
- 0.00
- 12.98
1.00

0.00
12.00
1.01
999.46
- 39.95
- 12.98
1.00

0.000
0.000
0.000
0.000
13,245.980
13,245.980
13,316.500

0.000
0.335
30.436
2.642
15,876.565
15,909.978
15,979.793

0.000
0.000
0.000
0.009
0.000
0.009
0.009

85.718
0.001
0.101
0.000
2,563.416
2,649.236
2,650.664

Component Flowrates (kg/batch)

B-Gal
Nitrogen
Oxygen
Proteins
Sodium Chloride
Water
WFI
TOTAL (kg/batch)
TOTAL (L/batch)

Component Flowrates (kg/batch)

B-Gal
Proteins
Sodium Chloride
Water
WFI
TOTAL (kg/batch)
TOTAL (L/batch)

- Page 13 -

4. OVERALL COMPONENT BALANCE (kg/batch)

COMPONENT

INITIAL

INPUT

OUTPUT

FINAL

IN-OUT

Ammonia
B-Gal
Biomass
Carb. Dioxide
Debris
Glucose
Nitrogen
Nucleic Acids
Oxygen
Phosphoric Acid
Process Water
Proteins
Salts
Sodium Chloride
Sodium Hydroxid
TRIS HCl
Water
WFI
TOTAL

0.000
0.000
0.000
0.000
0.000
0.000
300.943
0.000
91.360
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
392.304

395.000
0.000
0.000
0.000
0.000
6,143.001
44,891.805
0.000
13,628.282
529.703
75,838.947
0.000
1,025.999
1,099.398
2,107.576
13,095.071
0.000
637,300.038
796,054.821

39.798
109.757
13.496
4,489.470
765.927
122.860
44,958.445
510.618
10,525.142
529.703
79,123.937
1,164.137
183.179
1,099.398
2,107.576
13,095.071
2.651
637,300.038
796,141.204

0.014
0.000
0.000
1.556
0.000
0.000
234.303
0.000
70.048
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
305.921

355.188
- 109.757
- 13.496
- 4,491.025
- 765.927
6,020.141
0.000
- 510.618
3,124.453
0.000
- 3,284.990
- 1,164.137
842.820
0.000
0.000
0.000
- 2.651
0.000
0.000

- Page 14 -

5. EQUIPMENT CONTENTS
V-101
Procedure

Operation

P-1
P-1
P-1
P-1
P-1

START
CHARGE-1 (Charge)
CHARGE-2 (Charge)
TRANSFER-OUT-1 (Transfer Out)
CIP-1 (In-Place-Cleaning)

FR-101
Procedure

Operation

P-5
P-5
P-5
P-5
P-5
P-5

START
TRANSFER-IN-1 (Transfer In)
CHARGE-1 (Charge)
FERMENT-1 (Batch Stoich. Fermentation)
TRANSFER-OUT-1 (Transfer Out)
CIP-1 (In-Place-Cleaning)

V-102
Procedure

Operation

P-7
P-7
P-7
P-7
P-7
P-7

START
TRANSFER-IN-1 (Transfer In)
TRANSFER-OUT-1 (Transfer Out)
TRANSFER-IN-2 (Transfer In)
TRANSFER-OUT-2 (Transfer Out)
CIP-1 (In-Place-Cleaning)

V-103
Procedure

Operation

P-10
P-10
P-10
P-10

START
TRANSFER-IN-1 (Transfer In)
TRANSFER-OUT-1 (Transfer Out)
CIP-1 (In-Place-Cleaning)

DE-101
Procedure

Operation

P-12
P-12
P-12

START
FILTER-1 (Dead-End Filtration)
TRANSFER-OUT-1 (Transfer Out)

V-104
Procedure

Operation

P-13
P-13
P-13
P-13
P-13
P-13

START
TRANSFER-IN-1 (Transfer In)
TRANSFER-OUT-1 (Transfer Out)
TRANSFER-IN-2 (Transfer In)
TRANSFER-OUT-2 (Transfer Out)
CIP-1 (In-Place-Cleaning)

Time (in h)

Volume (in L)

0.000
0.700
1.022
7.022
8.856

0.000
64,340.728
70,606.248
0.000
0.000

Time (in h)

Volume (in L)

1.022
7.022
7.764
25.764
27.025
28.859

0.000
70,879.268
70,878.470
70,676.770
0.000
0.000

Time (in h)

Volume (in L)

25.764
27.025
35.025
35.025
51.025
53.859

0.000
70,676.673
0.000
35,193.672
0.000
0.000

Time (in h)

Volume (in L)

35.025
51.025
59.025
60.859

0.000
35,071.899
0.000
0.000

Time (in h)

Volume (in L)

51.025
59.025
59.025

0.000
1,440.448
0.000

Time (in h)

Volume (in L)

51.025
59.025
67.025
67.025
83.475
85.308

0.000
33,124.572
0.000
16,562.180
0.000
0.000

- Page 15 -

V-105
Procedure

Operation

P-16
P-16
P-16
P-16
P-16
P-16

START
TRANSFER-IN-1 (Transfer In)
TRANSFER-OUT-1 (Transfer Out)
TRANSFER-IN-2 (Transfer In)
TRANSFER-OUT-2 (Transfer Out)
CIP-1 (In-Place-Cleaning)

C-102
Procedure

Operation

P-18
P-18
P-18
P-18

START
AFTER AUTO-INIT
LOAD-1 (GFL Column Loading)
ELUTE-1 (Column Elution (Simplified))
WASH-1 (Column Wash (Simplified))

V-106
Procedure

Operation

P-19
P-19
P-19
P-19
P-19
P-19

START
TRANSFER-IN-1 (Transfer In)
TRANSFER-OUT-1 (Transfer Out)
TRANSFER-IN-2 (Transfer In)
TRANSFER-OUT-2 (Transfer Out)
CIP-1 (In-Place-Cleaning)

DF-101
Procedure

Operation

P-20
P-20
P-20

START
AFTER AUTO-INIT
DIAFILTER-1 (Diafiltration)
END

FDR-101
Procedure

Operation

P-21
P-21
P-21
P-21
P-21

START
TRANSFER-IN-1 (Transfer In)
DRY-1 (Freeze Drying)
TRANSFER-OUT-1 (Transfer Out)
CIP-1 (In-Place-Cleaning)

Time (in h)

Volume (in L)

67.113
83.641
89.641
89.641
99.762
101.596

0.000
4,867.842
0.000
1,622.764
0.000
0.000

Time (in h)

Volume (in L)

89.641
89.641
99.762
99.962
100.162

0.000
62.414
0.000
0.000
0.000

Time (in h)

Volume (in L)

89.646
99.962
105.962
105.962
106.962
108.796

0.000
5,326.600
0.000
2,663.300
0.000
0.000

Time (in h)

Volume (in L)

99.962
99.962
105.962
105.962

0.000
5,326.600
2,663.300
0.000

Time (in h)

Volume (in L)

105.962
106.962
121.962
131.127
132.960

0.000
2,663.300
2,650.664
0.000
0.000

- Page 16 -

C-101
Procedure
P-15
P-15
P-15
P-15
P-15
P-15
P-15

Operation
START
AFTER AUTO-INIT
LOAD-1 (PBA Column Loading
(Simplified))
ELUTE-1 (Column Elution (Simplified))
WASH-1 (Column Wash (Simplified))
WASH-2 (Column Wash (Simplified))
WASH-3 (Column Wash (Simplified))
WASH-4 (Column Wash (Simplified))

Time (in h)

Volume (in L)

67.025
67.025

0.000
2,070.272

83.475

0.000

83.641
84.141
84.975
85.308
85.725

0.000
0.000
0.000
0.000
0.000

- Page 17 -