Title:
Preparation & purification of racemic mixture of bi-naphthol and to
confirm its structure and purity by NMR Spectroscopy.
By:
Madhuri Mallela (Roll No. - 56)
Ashish Agarwal (Roll No. - 65)
Jyothi V. Nair (Roll No. - 72)
Harsh Purwar (Roll No. - 76)
1
Contents
Abstract 03
Keywords 03
Introduction 04
Apparatus Required 05
Chemicals Required 05
Theory 05
Procedure 06
Observations 06
Precautions 07
References 08
2
Abstract
In this experiment we synthesized and purified the Bi-naphthol from a very commonly
available compound β-naphthol or 2-napthol. β-naphthol reacts with FeCl3 to give a racemic
mixture of Bi-naphthol with FeCl2 and hydrochloric acid.
Chemical Reaction:-
β-naphthol Bi-naphthol
The above mentioned reaction is carried out in a 2-necked round bottom flask attached with
a reflux condenser and a dropping funnel with continuous & vigorous stirring and heating.
After the completion of the reaction the crude product is washed with the boiling water,
filtered, dried in air so as to remove the bi-products (FeCl2 & HCl) and unreacted reactants
and then finally re-crystallized with toluene.
The NMR spectroscopy was also done to check the purity of the crystals and to confirm the
structure of the purified organic compound (i.e. bi-naphthol).
Keywords:
Racemic mixture, chirality, stereo isomers, diastereomers, NMR spectroscopy, coupling
reaction, asymmetric catalysis, ligand, racemization, BINOL.
3
Introduction
1,1'-Bi-2-naphthol (BINOL) is an organic compound that is often used as a ligand for
transition-metal catalyzed asymmetric synthesis. BINOL has axial chirality and the two
enantiomers can be readily separated and are stable toward racemisation. The specific
rotation of the two enantiomers is +/- 35.5° (c=1 in THF). BINOL is a precursor for another
chiral ligand called BINAP.
Axial chirality is a special case of chirality in which a molecule does not possess a
stereogenic center (the most common form of chirality in organic compounds) but an axis of
chirality - an axis is about which a set of substituent is held in a spatial arrangement which is
not super-imposable on its mirror image.
The specific rotation of a chemical compound (α) is defined as the observed angle of optical
rotation θ when plane-polarized light is passed through a sample with a path length of 1
decimeter and a sample concentration of 1 gram per 1 deciliter. The specific rotation of a
pure material is an intrinsic property of that material at a given wavelength and
temperature. Values should always be accompanied by the temperature at which the
measurement was performed and the solvent in which the material was dissolved.
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Aim:-
To prepare the racemic mixture of bi-naphthol and to check the purity of the crystals by
NMR Spectroscopy.
Apparatus Required:-
1. Two-necked round bottom flask (500 mL)
2. Reflux condenser
3. Guard tube
4. Dropping funnel
5. Silicon Oil bath
6. Heater with magnetic stirrer
7. Beakers (250 mL, 50 mL)
8. Measuring cylinders ( 500 mL, 50 mL)
9. Stopper
Chemicals Required:-
1. 2-Naphthol (36 grams)
2. Ferric Chloride (70 grams)
3. Toluene
Theory:-
Chemical Reaction:-
β-naphthol Bi-naphthol
Two moles of β-naphthol or 2-napthol reacts with 2 moles of Ferric Chloride to give racemic
mixture of bi-naphthol.
Above reaction is an oxidative ortho-ortho coupling reaction catalyzed by Fe3+. Iron(III) ions
also stabilize the transition state.
5
Procedure:-
1. The apparatus is set and the chemicals are weighed as mentioned below:-
7.2 g of 2-napthol
14 g of FeCl3
2. 2-napthol is dissolved in 300 mL of water and heated in the 2-necked RB with the
reflux condenser connected to it.
3. After the 2-napthol is completely dissolved, FeCl3 dissolved in 30 mL of water is
added to it drop-wise, using the dropping funnel with vigorous stirring.
4. At the end of the reaction bi-naphthol separates out in flakes.
5. It is boiled for 5-10 minutes and the hot suspension is filtered through Buchner
funnel.
6. Then it is washed with boiling water till the filtrate becomes colourless.
7. The residue is then left to dry in air upon a filter paper.
8. This process is repeated 5 times to get the desired amount of bi-naphthol.
9. The crude product is then re-crystallized using toluene.
10. The crude product is dissolved in toluene and refluxed.
11. The dissolved product is filtered using Buchner funnel and then using activated
charcoal.
12. The process is repeated till we obtain clear crystals of bi-naphthol.
Observations:-
Zoomed In
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Explanation of the NMR spectroscopy results:-
There are 14 hydrogen molecules in a molecule of bi-naphthol out of which two are
attached with the oxygen which give a broad peak in the NMR. This is because of the proton
transfer between O-H and CDCl3 (solvent). This
effect is also called as the tumbling effect.
The other 12 ‘H’ atoms give six different peaks
due to symmetry. The doublet or triplet is
observed due to difference in their chemical
environment or chemical shift.
Hydrogen H(1)s are similar with respect to their
chemical environment. Due to the electron
withdrawing nature of the OH group, these
hydrogen’s suffer the highest chemical shifting.
And as spin value for the proton is ½ and the
number of 3 bond apart hydrogen’s are two so we get doublet corresponding to H(1)’s.
Similarly we could easily figure out the following results:
As only the peaks corresponding to the hydrogen’s in the compound (bi-naphthol) are
observed in the NMR spectroscopy we can say that the compound prepared is pure.
Precautions:-
1. The apparatus should be rinsed properly before use and then dried where necessary.
2. The reaction mixture should be heated and stirred properly.
3. Proper stirrer should be used according to the requirement of the apparatus.
4. FeCl3 should be added to the round bottom flask slowly using the dropping funnel
and vigorous stirring.
5. Toluene and other volatile and hazardous chemicals should be handled with extreme
care. It is a carcinogenic volatile liquid.
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References
Vogel’s Textbook of Practical Organic Chemistry
– By Brian S. Furniss
www.wikipedia.org
www.google.co.in
www.about.com
Organic Chemistry
- By Jonathan Clayden