Biochemistry is a field of enormous E} y; m wp K f k;; fascination and utility, arising, no doubt, LEHNINGER from our own self-interest. Human PRINCIPLES OF BIOCHEMISTRY welfare, particularly its medical and Vth Edition nutritional aspects, has been vastly David L. Nelson Michael M. Cox improved by our rapidly growing understandings of Biochemistry. Indeed, scarcely a day passes without the report of biomedical discovery that benefits a significant proportion of humanity. Further advances in this rapidly expanding field of knowledge will no doubt lead to even more spectacular gains in our ability to understand nature and control our destinies. Professor, Professor, This book is intended to provide with B.A., St. Olaf B.A., University of a through grounding in biochemistry. In College; Delaware the Twenty six years since the first Ph.D., Stanford Ph.D., Brandeis Medical School University edition of Principles of Biochemistry was published by Albert Lehninger, has 571B Biochemistry 341B Biochemistry Addition Addition served as the starting point and the Department of Department of model for the four subsequent editions. Biochemistry Biochemistry 433 Babcock Drive 433 Babcock Drive Before twenty five years ago not a Madison, Madison, single genome have been sequenced, WI 53706-1544. USA WI 53706-1544. USA not a single membrane protein had been solved by crystallography, and not a single knockout mouse existed. Ribosome had just been discovered, PCR technologies introduced the field of biochemistry has continued its phenomenal and rapidly accelerating growth. This remarkable expansion of our knowledge, the work of thousands of talented and dedicated Scientist, has been characterized by numerous enrichment of almost every aspects of the field.
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th This 5 edition contains the structural confirmation on FAS I. photographs of 31 Nobel laureates who (Chapter 21) have received their prizes for o New section on expanded understanding of the role of RNA in Chemistry/Physiology/Medicine since that cells. (Chapter 26) first edition of Principles of Biochemistry. o New information on the roles of Lehningers Biochemistry is the RNA in protein biosynthesis. threshold of a new molecular physiology in (Chapter 27) which process such as membrane excitation secretion, hormone action, BIOCHEMICAL METHODS INCLUDED vision, gestation, olfaction, respiration, An appreciation of biochemistry often muscle contraction and cell movement will requires an understanding of how be explicable in molecular terms and will biochemical information is obtained. become accessible to genetic dissection Some of the new methods or updates and pharmacological manipulation. described in this edition are. RECENT ADVANCES INCLUDED o Use of MALDI-MS in determination o New discussion of AMYLOID of oligosaccharide structure. DISEASE in the context of protein (Chapter 7) folding (Chapter 4) o Forensic DNA analysis, a major o New section on Pharmaceuticals update covering Modern STR developed from an understanding of analysis. (Chapter 9) enzyme mechanism, using Penicillin o More information on microarray and HIV protease inhibitor as example. (Chapter 9) (Chapter 6) o Use of tags for protein analysis and o New discussion of on sugar analogs purification. (Chapter 9) as drug that target viral o Development of bacterial strains neuraminidase. (Chapter 7) with altered genetic codes, for site o New material on Green fluorescent specific insertion of novel amino protein. (Chapter 9) acids into proteins. (Chapter 27) o New section on liposome’s. (Chapter 10) In this edition each and every chapter o New section on emerging role of has been thoroughly revised and ribose 5 phosphate as a central updated to include the most important regulator of glycol sis and advances in Biochemistry. gluconeogenesis. (Chapter 15) o New section on role of Transcription Mr. M. Prasanna Rajeshkumar factor (PPARs) in regulation of lipid Lecturer in Biotechnology catabolism. (Chapter 17) o Revised and updated section of fatty acid syntheses including new tpbay; khj ,jo; - 15 9 brk;gh;; 2009 f tp ij MWtopr; rhiyNahuj;jpy; mbgl;L caph;fF NghuhLfpwhh;h; ; g; NghuhLfpwh ‡ m`pk; ; rapd; epiy ‡ m`pki Kjpath;. V. uQ;rpj;> mtiuj; mti uj; jhz;br; nry;fpwhd; ,sq;fiy Kjyhz;L Mq;fpyk; ve;j xU nghWg;Gkpy;yhj ,isQd;. vspath;fs; Ve;Jk; tYtpyhf; iffisg; iffisg; Gwf;fzpj;J NfhapYf; NfhapYf;Fy; nrd;W cz;baypy; NghLfpwhd; Nfhb &gha;. hNt!! X kfhj;khNt tpLjiyf;fhf hfg; g; ghLgl;L tPukuzk; cw;whYk; vk; tPLk; ehLk; tsk; ngwr; nra;jhP !;
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$l;lk; eilngUk;. ,dpg;Gfis ‡fpwp fpwpj; fpwpj;Jtg;; gz;bif‡ if gfph;eJ ; cz;gh;. F. ejpaurp> gpwh; eyj;jpw;fhfj; jpahfq;fs; gy ,sq;fiy Kjyhz;L Mq;fpyk; nra;j ,NaR fpwpj;Jtpd; gpwe;jehs; midtUf;Fk; md;igAk; jpah fpwpj;Jt kjj;jpd; flTshff; fj;ijAk; Nghjpf;Fk; ehshFk;. fUjg;gLgtUk; jpahfj;jpd; jpU mth; fhl;ba topapy; ehKk; TUtkha; tpsq;FgtUkhd ,NaR elg;Nghk;. fpwpj;Jtpd; jpUehNs fpwpj;Jtj;; jpUehshff; nfhz;lhlg;gLfpwJ. ‡ ,NaRtpd; nghd;nkhopfs; ‡ A+jh;fSk; ,d;Dk; rpykf;fSk; gy J. RNu~;> %lek;gpf;iffisAk; Raeyj;ijAk; ,sq;fiy Kjyhz;L Mq;fpyk; nfhz;bUe;jdh;. ,jdhy; rKjhak; rj;jpaj;jpw;F tpNuhjkha;g; ngha; gy ,d;dy;fSf;F Mshapd. nrhy;yhkypUq;fs;. mf;nfhLikia xopf;Fk; nghUl;L fh;jj ; Uf;F Kd;ghfj; jho; n[UrNyk; efhpy; fd;dp khpahSf;F ikg;gLq;fs;> mg;nghOJ mth; kfshf mtjhpj;jth; ,NaRngUkhd;. cq;fis cah;jJ ; thh;. rpy E}W tUlq;fSf;F Kd;Gtiu tpUe;J tPlL ; f;Fg; NghtjpYk; Jf;f fpwpj;Jtj;; jpUehs; gy;NtW ehLfspy; tPlL; f;Fg; NghtJ eyk;. ,jpNy gy;NtW jpdq;fspy; nfhz;lhlg;gl;L vy;yh kdpjhpd; KbTk; fhzg;gLk;. capNuhbUf;fpwtd; ,ijj; jd; te;jJ. Mdhy; cyfpYs;s fpwpj;Jt kdjpNy rpe;jpg;ghd;. rigapdh; midtUk; xd;W$b brk;gh; xU fhhpaj;jpd; Jtf;fj;ijg; 25Mk; ehis fpwpj;J gpwe;j ehshf ghh;ff ; pYk;> mjd; Kbit Nahrpg;gJ mwptpj;jdh;. ey;yJ. ngUikAs;stidg; ghh;ff ; pYk;> nghWikAs;std; cj;jkd;. fz;zN P uhNl tpijf;fpwth;fs; fk;guP j;NjhNl mWg;ghh;fs;. Nrhk;NgwpNa> eP vWk;gpdplj;jpy; Ngha;> mjd; topfisg; ghh;jJ ; > RWRWg;igf; fw;Wf; nfhs;. xUtd; cd;id tyJ fd;dj;jpy; miwe;jhy;> mtDf;F kW fd;dj;ijAk; jpUg;gpf; nfhL. brk;gh; khjk; cyfpd; gyghfq;fspy; Fsph; fhykhFk;. mf;fhy fl;lj;jpy; gy fpwpj;Jth;fs; ,uT gy ghly;fis fh;jj ; h; $wpa nghd;nkhopfisf; ,irj;jgb xt;nthU tPLfspYk; filg;gpbj;J tho;Nthk;. mid nrd;W tho;j ; J ; njhptpf;fpd;wdh;. tUf;Fk; fpwpj;Jgpwe;jehs; tho;j; tPLfspy; gy tz;z tpsf;Ffis Jfs;. vhpatpLfpd;wdh;. fpwpj;J gpwe;j ehsd;W Nguhyaq;fspy; gpuhh;jj ; id tpbay; khj ,jo; - 15 11 brk;gh;; 2009 0.48%. Much higher rates are found F W q; f l; L iu among people attending STD clinics ‡AIDS‡ (3.6%), female sex workers (5.1%), Started on 1st December 1988, World injecting drug users (7.2%) and men AIDS Day is about raising money, who have sex with men (7.4%). increasing awareness, fighting prejudice AIDS Stands for Acquired Immune and improving education. The World AIDS Deficiency Syndrome. Acquired means Day theme for 2009 is 'Universal Access you can get infected with it Immune and Human Rights'. World AIDS Day is Deficiency means a weakness in the important in reminding people that HIV has body’s System that fights diseases. not gone away, and that there are many Syndrome means a group of health things still to be done. problems that make up a disease. AIDS is caused by a virus called HIV get According to UNAIDS estimates, Infected with HIV. Your there are now 33.2 million people body will try to fight the living with HIV, including 2.5 million infection. It will make children. During 2007 some 2.5 “Antibodies”, special million people became newly molecules. infected with the virus. Around half of all people who become infected A blood test for HIV looks with HIV do so before they are 25 for these antibodies. If you and are killed by AIDS before they have them in your blood, it are 35. means that you have HIV infection.People who have the HIV Around 2.3 antibodies are million people in cyf mstpy; brk;gh; 1Mk; Njjp called “HIV- India are living va;l] ; ; tpopg;Gzh;T ehshf Positive”. mwptpf;fg;gl;Ls;sJ. with HIV. Of these, an jkpo;ehL va;l;]; fl;Lg;ghl;Lr; You don’t estimated 39% rq;fj;jpd; jpl;l ,af;Feh; jpU. actually get are female and tp[aFkhh;> “2001Mk; Mz;Lf; “AIDS”. You fzf;nfLg;gpd;gb> jkpof kf;fs; 3.5% are njhifapy; vr;.I.tp. Nehahy; might get children. The ghjpf;fg;gl;lth;fs; 1.25 tpOf;fhL infected with average HIV ,Ue;jdh;. jw;NghJ 0.26 tpOf;fhlhf hlhff; f; HIV and later Fiwe;Jtpl;ldh;” vd;W kfpo;rr ; pahd prevalence fUj;ijj; njhptpj;Js;shh;. you might among women develop AIDS. attending antenatal clinics in India is The blood, vaginal fluid, semen, and
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breast milk of people infected with HIV has enough of the virus in it to infect other F W q; f l; L iu people. Most people get the HIV virus by : Having sex with an infected person. ‡ Rdhkp ‡ Sharing a needle with someone whe’s F. ejpaurp> infected. being born when their mother is ,sq;fiy Kjyhz;L Mq;fpyk; infected or drinking the breast milk of an ,e;jpa kf;fs; ,Jtiu Nfl;buhj infected women. ngah; Rdhkp. 2004 Mk; Mz;L brk;gh; khjk; ,e;jpah kl;Lkd;wp You might not know if you get infected cyfk; KOtJk; by HIV some people get fever, Headache, nra;jpj;jhs;fspYk; jpUk;gj; jpUk;gf; sore muscles and joints, stomach ache, $wg;gl;lJ. swollen lymph glands, or a skin rash for Rdhkp vd;gJ [g;ghdpa thh;ji ; j. one or two weeks. Most people thing it’s Nguopit cz;lhf;Fk; uhl;rj fly; the flu. Some people have no symptoms. miyfis [g;ghd; nkhopapy; Rdhkp vd;W miog;gh;. HIV disease becomes AIDS when your 2004 Mk; Mz;L brk;gh; khjk; immune system is seriously damaged. If 26Mk; Njjp Rdhkp vd;dk; ,aw;ifr; you have loss than 200 CD4 Cells or if rPww ; j;jhy; Mapuf;fzf;fhd kf;fs; your ID4 percentage is less than 14% you khz;ldh;. have AIDS. See you have AIDS. There is fpof;fpe;jpa jPthd Rkj;uhtpy; an “official” list of these opportunistic kpff; Nfhukhd ghjpg;ig Vw;gLj;jpa Rdhkp miyfs; jkpofj;jpy; infections put out by the centers for ehfg;gl;bdk;> fhiuf;fhy;> Disease control (CDC). The most common fd;dpahFkhp> Fsr;ry;> Nfushtpy; ones are: PCP – a lung infection , KS – a rpy flw;fiug; gFjpfspYk; skin cancer , CMV – an infection that ehrj;ij Vw;gLj;jpd. fly; usually affect the eyes. miyfs; ngUk; rPww ; k; nfhz;L jiug;gFjpf;F te;jjhNyNa ,e;jg; There is no cure for AIDS. There are NguopT Vw;gl;lJ. Rkhh; 100 drugs that can slow down the HIV virus Mz;LfSf;Fg; gpd;dh; ,g;NguopT and Slow down the damage to your Vw;gl;Ls;sJ. immune system. There is no way to “clear” fhuzk;: flYf;Fs; epyeLf;fk; the HIV out of you body. Vw;gLk;NghJ flYf;Fs; ,Uf;Fk; - N. Priya, I -BA English Thanks : http://www.avert.org/ epyj;jpy; rpy,lq;fs; NklhfTk;> rpy ,lq;fs; jho;eJ ; k; NghFk;. ,e;j jpBh; khw;wj;jhy; fly;ePh; kl;lk; rpy ,lq;fspy; cah;eJ ; k;> rpy
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,lq;fspy; jho;eJ ; k; NghFk;. fly; ePh; Tsunami, Asian Tsunami, Indonesian kl;lk; caUk;NghJ me;jg; gFjpfspy; Tsunami and Boxing Day Tsunami. uhl;rj miyfs; tPRk;. mt;thW tPrpa Meganitude: 9.3 Mw, Depth: 30 Km uhl;rj miyfs; fliy xl;ba (19mi) epyg;gug;gpw;Fs; GFe;J Nguopit Epicenter Location: 3.316 N 95.854 E, cz;lhf;Fk;. mJNghy flYf;Fs; Type: undersea, Countries/regions epyk; jho;eJ; NghFk; ,lq;fspy; Affected: Indonesia (mainly in Aceh), Sri fly;ehP k ; l;lk; mkpo;eJ ; NghFk;. me;j Lanka, India (mostly in T.N), Thailand and ,lq;fspy; fly; ePuhdJ epyg;gug;ig Maldives. tpl;L cs;thq;fpg; NghFk;. The U.S Geological Surway initially eltbf;if: ,aw;ifapd; rPww; khd recorded the toll as 283,100 killed, epyeLf;fk;> kio> ,b Nghd;wtw;iw 14.100 missing, and 1,126,900 people Kd;$l;bNa mwpAk; rhjdq;fis displaced. However, more recent flw;fiu gFjpfspy; mikf;f Ntz;Lk;. analysis compiled by the United Nations flw;fiuNahuq;fspy; tho;gth;fF ; lists a total of 229,866 people lost, Nguiyfs; jhf;fhj tz;zk; including 186,983 dead and 42,883 FbapUg;Gfis toq;f Ntz;Lk;. ,J missing. Nghd;w eltbf;iffis murhq;fk; Nkw;nfhz;Ls;sJ. NkYk; Mo;Foha; fpzWfs; Njhz;Ljy;> epyj;jpDs; ,Uf;Fk; rkepiyia Fiyj;jy; Nghd;w kdpjdhy; cUthf;fg;gLk; ,jo; - 16> rdthp 2010 Neh;fhzy; Nrjq;fis epWj;Jtjd; %yk; ek;ik o ftpQh; f. Nkhfduq;fd; - <NuhL ehk; ,aw;ifr; rPww ; q;fspypUe;J nry;tp.n[.ghujp> jkpo;jJ ; iw tphpTiuahsh;. fhg;ghw;wpf; nfhs;syhk;. rpwg;Gf; fl;Liufs; o NeNdh njhopy; El;gk; - mwpKfk; 2004 INDIAN OCEAN EARTHQUAKE jpU.gpurd;duhN[];Fkhh;> N. Priya , I BA English tphpTiuahsh;> caph;jn; jhopy; El;gj; Jiw. o khyjp ikj;hpapd; ngz;zpathpfs; The 2004 Indian Ocean earthquake was nry;tp.r.ghf;fpayl;Rkp> jkpo;jJ ; iw tphpTiuahsh;. an undersea mega thrust earthquake that o vjph;fhyj;jpy; kdpjDk; kUe;Jk; accurred at 00.58.53 UTC on December jpU. K. uhk;Nkhfd;>; KJepiy 26, 2004, with an epicenter off the west tphpTiuahsh;> caph;jn ; jhopy; El;gj;Jiw. coast of Sumatra, Indonesia, The zuake o ePuopT Neha; - tphpthd tpsf;fk; itself is known by the Scientyfic Community Kidth; [p.gp. nre;jpy; Fkhh;>; tphpTiuahsh;>caph;Ntjpapay;J ; iw. as The Sumatra-Andaman earth Quake. The resulting itself is given various names, including the 2004 Indian Ocean