Name_____________________________________________ Grade /20

Living Environment Redo Grade /20

Lab # _________

TITLE- Pond Water under the Microscope

OBJECTIVE- As you know, the building
blocks of life are cells. Prokaryotic cells
are those cells that do NOT have a
nucleus. They mostly include bacteria and
archaea. These cells do not have
membrane bound organelles.
Eukaryotic cells are those that have a
true nucleus. That would include plant,
animal, algae and fungal cells. As you can
see in the picture, eukaryotic cells are
typically larger than prokaryotic cells.
In this lab, we will look at examples of both prokaryotic and eukaryotic unicellular
organisms, most of which are commonly found in pond water


MATERIALS- Microscope, Slides, Coverslips, Pond Water

PROCEDURE
1. Obtain a sample of pond water and place it on the slide. Cover the slide with a
coverslip so that there is no air bubbles trapped.
2. View your slide under low power. You may need to move the slide around before
you can locate a microorganism.
3. Once you have located a microorganism you should draw it. Remember all
drawings should be as accurate as possible.
4. Fill out the information about your organism next to your drawing as best as you
can. Use the reference sheet on the last page of this lab to try to identify your
organism.
a. Field Size- Refer to measuring with the microscope lab for field of view side
b. Object size- estimate how many would fit across your field of view. Divide
the number of um of the field size by the number of species that would fit.
This is your object size.
c. Method of locomotion (motile or sessile)
d. Specific organelles- list all organelles you can see




Ocular Magnification- _________________

Objective Magnification- ______________

Total Magnification- ___________________

Field Size (um)- ________________________

Object size (um) - _____________________
Method of locomotion- _______________
Shape - ________________________________
Color- _________________________________
Specific organelles- ___________________
Specimen name- ______________________





Ocular Magnification- _________________

Objective Magnification- ______________

Total Magnification- ___________________

Field Size (um)- ________________________

Object size (um) - _____________________
Method of locomotion- _______________
Shape - ________________________________
Color- _________________________________
Specific organelles- ___________________
Specimen name- ______________________



Ocular Magnification- _________________

Objective Magnification- ______________

Total Magnification- ___________________

Field Size (um)- ________________________

Object size (um) - _____________________
Method of locomotion- _______________
Shape - ________________________________
Color- _________________________________
Specific organelles- ___________________
Specimen name- ______________________





Ocular Magnification- _________________

Objective Magnification- ______________

Total Magnification- ___________________

Field Size (um)- ________________________

Object size (um) - _____________________
Method of locomotion- _______________
Shape - ________________________________
Color- _________________________________
Specific organelles- ___________________
Specimen name- ______________________


Read the information below. Then answer the questions that follow based on what your
read and your knowledge of biology.


How did the first eukaryotes evolve?
Scientists think that the first eukaryotes may have
begun as large prokaryotic cells. The DNA of some
eukaryotic organelles is very similar to the DNA of
bacteria. Because the DNA is similar, scientists think
that the organelles may have once been bacteria.
These bacteria enter the larger prokaryotes. The
bacteria were able to live in the larger cell, and they
because the organelles of the larger cell.
Green photosynthetic bacteria probably
became the chloroplast that you see in plant cells
and in photosynthetic protest cells. Other bacteria
became the mitochondria found in all eukaryotic
cells.

Analysis Questions
1. Which type of organism was present first;
Eukaryotes or Prokaryotes? Support your
answer.





2. Describe what the image above is trying to represent.





3. What evidence do we have to suggest that prokaryotic organisms became
the cellular organelles of eukaryotic organisms?





4. Did you see any organisms in the pond water that would be similar to the
prokaryotic organisms that became the mitochondria or chloroplast? Explain.