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Microscope Lab

Name:

Per:

Question: Can we see the difference between Plant and Animal cells under a
microscope?

If we can, what differences are there?

Background Information: Read this information, Answer the questions below.


-Plant and Animal cells are both Eukaryotic (Eu= true, Karote= kernel) meaning they
both have a Nucleus.
-Plant cells have a rigid Cell Wall and often have a similar size and shape. Animal cells
are more Asymmetrical
-Plant cells also have chloroplasts, a organelle with green pigment that converts
sunlight to sugar.
Finally, Plant cells have a large central vacuole that
stores water for the cell.

-Microscope magnification is determined by multiplying


the power of the Eye Piece lens by the Objective lens. Example: An eye piece with 5x
power and a objective lens of 10x power.

Questions:
1. What type of Cell are both Plant and Animals? They are both an eukaryotic cell.

2. What are 3 characteristics of plant cells that you can see under a microscope?

Three characteristics of plant cells you can see under a microscope are their nuclei,
their chloroplasts, and their larger central vacuole.
3. What is the Magnification of an eye piece of 10x and an objective lens of 5x? 50x

Hypothesis- When using a compound light microscope to identify cells, if the cell has
____a nucleus __________________________________________________, then it is
a __eukaryotic _____________________ cell.

Procedure- Part 1 (indentifying the parts of a microscope)


-Use the labels on the lab station to label your microscope. (The Instructor or TA must
sign-off that you have completed this task with 100% accuracy.)

Sign-Off:___________________________________

Procedure Part 2: Making a wet mount slide and operating a Compound Light
Microscope.
1. Cut a letter e out of a newspaper or magazine.
2. Make a wet mount Slide
Place the letter e in the middle of the slide.
Place a drop of water on top the letter e.
Place a cover slip over the letter.
3. Take data.
A. Make 3 Circles on a separate sheet of paper with a Petri dish.
B. Mark them with the total magnification at the top of the circle.

C. Using the microscope, draw what you see at each magnification level. There
should be 3 data pictures for each sample.

Procedure Part 3- Animal Cells


1.Gently rub a stir stick on the inside of your cheek or at least 15 seconds.
2.Gently rub it ONE time on the slide. Creating a smear. Throw away the
stirrer.
3. Gently place the cover slip gently over your smear.
4.Add a drop of iodine stain to the edge the cover slip. Tap gently on the cover
slip with the eraser of a pencil to remove air bubbles.
5.Place the slide on the stage of the microscope.
6.Take Data (see procedure in part 2)

Procedure Part 4- Plant Cells


1. Obtain a prepared slide with onion skin from the teacher.
2. Gently place a cover slip over the onion skin.
3. Add a drop of Metholine Blue to the edge of the cover slip. Gently tap the cover
slip with a pencil eraser to remove bubbles.
4. Place the slide on the stage of the microscope.
5. Take Data. (see procedure in part 2)

Conclusion: Write a 3 part conclusion for the question and hypothesis above.
(A 3 part conclusion includes; describing the data in words, your analysis of the data and a
statement of the validity of you hypothesis (was your hypothesis correct) and why. Each different
slide showed lots of detail about what I was looking at for example in the cheek cell it showed
there was a lot of clumps of stuff and in the onion slide there was what looked like a brick wall
and you could see a nucleus. My analysis of that data is that only the cheek and onion wet
mount slides were actual cells. My hypothesis was correct because the cells with nuclei we're
eukaryotic.

Reflection Questions:
1.

Why was it necessary to stain the cells?

It was necessary to stain the cells because if you didn't you wouldn't be able to see any of the
stuff inside the cell as well.
2. What is the magnification of:
a. an eye piece of 10x and an objective of 4x? 40x
b. an eye piece of 10x and an objective of 10x? 100x
c. an eye piece of 10x and an objective of 40x? 400x
3. Complete the Venn Diagram comparing Plant cells and animal cells using these terms. (you
may have to do some outside research)
Many small Vacuoles, Central Large Vacuole, Cell Wall, Cell Membrane, Nucleus, Chloroplast
Type to enter text

Animal Cells only: Many small vacuoles


Plant Cells only, central
large vacuole,
Both: Cell Wall,
chloroplast
Cell Membrane,
.
.
nucleus