Date:

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Practical:

Aim: To prepare and evaluate stable topical suspension of

hydrophobic API with high amount of solid and compare with
marketed formulation.
Formula:
Packaging: plastic bottle
Pack size: 60 ml bottles
Formulation:
Ingredients
Calamine
Zinc oxide
Bentonite
Sodium citrate
Liquid phenol
Glycerin
Rose water

Quantity taken
9g
3g
1.8g
0.3g
0.9g
3ml
60ml

Role of Ingredient
API, Protective agent
Astringent
Suspending agent
Viscosity enhancer
Preservative
Humectant
Vehicle

Marketed formulation:
Calamine suspension(Zincal):
Company Name: Alive Manufacturer
Content: Calamine IP150 gm
Zinc oxide IP50 gm
Bentonite IP30 gm
Glycerin IP50 ml
Sodium citrate IP- 5 gm
Liquid phenol IP- 5 ml
Purified water q.s.-100 ml
Volume: 100 ml

Procedure:
Dissolve Sodium citrate with 1/4th quantity of water.
Mix Calamine, Zinc oxide & Bentonite with Sodium citrate solution.
Dilute above mixture with some quantity of water.

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Observation
1. Sedimentation Volume Ratio(SVR)
Suspension

Calamine
lotion

Time
(min)

Initial
Height
(H0)

Ultimate
Height(Hu)

SVR

LF

LF

LF

MF

MF

0
10
20
30
40
50
60

LF: Laboratory formulation

MF: Marketed formulation

2. Particle size distribution:

Calibration Factor:
No. of divisions of stage micrometer= 10(y)
No. of divisions of eye piece micrometer=7.1(x)
(y/x)*10= 10/7.1*10
= 14.08m

For lab. formulation:

RANGE

MEAN(d)

NO. OF
PARTICLES(f)

f*d

Average
SVR
MF LF

MF

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Add Liquid phenol & Glycerin to above mixture and mix well.
Adjust final volume with water.

Evaluation parameters:
1.
2.
3.
4.
5.
6.

Sedimentation Volume Ratio(SVR)

Particle size distribution
Density
Redispersibility
Viscosity
Appearance

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3. Density:
A. Calamine lotion of LF:
Initial weight of empty bottle W0 =
Bottle + suspension W1 =
Bottle capacity = 10 ml
Density = W1-W0
10

B. Calamine lotion of MF:

Initial weight of empty bottle W0 =
Bottle + suspension W1 =
Bottle capacity = 10 ml
Density = W1-W0
10
4. Redispersibility

5. Viscosity

Result:
PARAMETER LF
SVR
Particle size
distribution
pH value
Density
Redispersibility
Viscosity

MF

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Comment:
1. Topical suspension having high amount of solids should have minimum
sedimentation throughout the shelf-life.
2. In present study, attempt is made to prepare efficient stable suspension of
high amount of solid and compare with marketed formulation we
conclude from observed parameters that marketed formulation were
found to be more stable and efficient.

3.

Reference:
1. Pharmaceutical Dosage Form: Disperse system, Leon Lachman, Herbert
Lieberman, volume 2.