Sc k ion

(Ion Chromatography IC)

Nguyn l

Sc k (tin hnh bi M. Tswett)

ether

chlorophyll

Chromatography
colors

CaCO3

V d so snh
Ct nh
Si nng

Dng nc

y sng

Pha ng / Pha tnh

Dng chy

Pha ng

Lc tng tc

Pha tnh

Tng tc: trng lc ma st gia cc ht ct si v y sng

5

Chromato-graphy / -graph / -gram /

-grapher

Chromato (-graphy)
Chromato (-graph)
Chromato (-gram)
Chromato (-grapher)

: Phng php
: Thit b
: Sc (kt qu)
: Ngi lm sc k

Sc k lng
Pha ng: Cht lng
Pha tnh: Cht lng hoc cht rn
Mu ho tan trong pha ng c th c
tch v xc nh nng .

Sc k ion

Mt ng dng ca sc k lng phn tch

cc ion trc tip trong mu
Pha tnh: Ht nhi (cht mang) c gn cc
nhm trao i ion. V d: nhm amin bc 4
(cho anion), polybutadiene/maleic acid
copolymer (cho cation) v.v
Pha ng: Dung dch m ph hp. V d:
methanesulfonic acid, p-hydroxybenzoic acid
v.v
8

Trao i ion thi gian lu

Nhm trao i ion

Yu

F-

Mnh

NO3-

Sc (Chromatogram)

tR
Signal

Peak

h
A

tR : Thi gian lu
(Retention time)
A : Din tch (Area)
h : Chiu cao (Height)

Time
Injection of sample
10

Phn cng (hardware)

11

S dng chy (Flow-line)

Van bm
mu

L ct
Ct tch

Detector

Bm

Dung mi

Thi
B x l s liu

12

Bm dung mi

Motor & Cam

Pump head

Check valves

Plunger
Plunger seal
13

Thit k bm 2 piston
(Dual plunger)
Check valve

Parallel

Plunger head
14

Thng s chnh cho bm

Ch dng
Isocratic

flow / Binary gradient

Tc dng
Isocratic

flow: A.Flow
Binary gradient: T.Flow, B.Conc

Pressure
P.Max,

P.Min
15

Van bm mu
(Rheodyne 7725 / 7725i)
T bm

n ct

T bm

n ct

LOAD
T bm

n ct

T bm

n ct

INJECT
16

Bm mu vo vng mu

T bm

n ct

Sample Loop

LOAD

T bm

n ct

Sample Loop

INJECT
17

Phng php bm mu

Bm 1 phn vng mu
Th tch mu bm vo van do syringe quyt nh.
Th tch mu bm c gii hn n 1/2 th tch
vng mu

Bm y vng mu
Th tch mu bm vo van do vng mu quyt
nh.
Th tch mu bm vo van bng 2 5 ln th
tch vng mu loi b ht dung mi tn ti trc
trong vng mu.

18

L ct

Bo m nhit ct tch l n nh
Detector dn: dn in ph
thuc nhit cell t trong l ct
Gia nhit tun hon khng kh

19

Thng s chnh cho l ct

Oven temperature
T.Max

20

Detector dn

Detector dn CDD-10A: o dn
in ca dng dung mi pha ng i ra
t ct tch, pht hin cc ion

21

B iu khin h thng
(System controller)

System controller

SCL-10AVP
Giao tip vi phn mm trn PC, v iu
khin cc module ca h thng

22

Phn mm - LCsolution
C tc dng iu khin h thng sc k
Thu tn hiu t h thng sc k.
X l s liu phn tch
Nhiu thng tin v tin ch
Tuy nhin s dng vn n gin: nhp
mu bm mu x l kt qu

23

Phn mm - LCsolution

24

Trc khi phn tch

25

Pha ng

Nc

Ultrapure water
HPLC grade
18 M/cm

Dung mI hu c

HPLC grade
Mt s ng dng i hi
super-high grade.

26

Thay dung dch m

Dung dch m 1 (Buffer1) trong sc k ion

khng c php thay th trc tip bng dung
dch m 2 (Buffer2).
Phi qua trung gian l nc
Buffer 1

Water

Buffer 2
27

Loi kh

Cc vn gy ra bi kh ho tan trong dung

mi
Bm khng tht u
Nhiu ln v tri nn xy ra trong cell ca detector

Dung mi cn c loi kh phng trnh cc

vn ny.

28

Loi kh & lc dung mi

Thot kh

Mng lc
(C: 0.45 um)

Chn khng

My lc siu m

29

Phn tch nh tnh v nh lng

30

Phn tch nh tnh

Bm mu chun
Bm mu th
So snh thi gian lu

31

Phn tch nh lng

So snh din tch peak hoc chiu cao
peak ca mu chun v mu th
Lp ng chun vi mu chun

Ngoi

chun
Ni chun

32

Phng php ng chun

Concentration

Area
A1

Calibration curve

C1

A2
C2

Peak area

A4

A3
A2

A3
C3

A1
A4

C4

C1

C2
C3
Concentration

C4
33

Shimadzu HPLC history (1978 to 1984)

World's 1st
Micro LC System

LC-3A

1984
Technology
Breakthrough
Compact, versatile HPLC
system with modular design
and centralized controller

1978
Technology
Breakthrough
Constant displacement
quick return (CDDR)
solvent delivery system

Shimadzu LC boasted
in International market

LC-6A
1981
Technology
Breakthrough
Totally microprocessor
controlled
ternary gradient HPLC
with autosampler

1982
First
Dedicated HPLC system
with 1 mm micro bore
column

LC-5A

LC-4A

1982
First
High resolution
High sensitivity dual lamp
PDA detector

SPD-M1A
34

Shimadzu HPLC history (1991 to 1996)

1995
Unparalleled
Worldwide
Acceptance
More than 50,000
LC-10A units shipped
since introduction

1991
First
Advanced modular HPLC
system with exclusive
fiber optic interface

LC-10A

1996
Performance
Breakthrough
Patented high stability,
4-partitioned photodiode
technology refractive
index detector

RID-10A

1996
Performance
Breakthrough
High sensitivity PDA
and fluorescence detector

SPD-M10AVP
RF-10AXL

1996
Productivity
Breakthrough
Autosampler options
for combinatorial drug
discovery and complete
system automation

SIL-10AXL
35

Present Shimadzu HPLC Products

CLASS-VP

Dedicated and high-function

HPLC only

Modular LC LC-VP

Research and development

System expandability

LCsolution

pharmaceutical

LC/LC-MS integrated S/W

both LC/LC-MS control

All-in-one LC LC-2010

Quality control
Routine analysis

36