'S7
~ '~N ~ I r- ~ ~~ I A L
INTER-OFFICE MEMORANDU ~W ~ ~.
To : Mr . Edward A . Vassallo
, The procedures for the reuse of the enzyme have been studied using
the column method . An enzyme concentration equivalent to a 700 ml batch
of syrup was bound to DEAE-cellulose . The bound enzyme was inserted in a
jacketed glass column and the column was heated by passing water at 60°C
t hrough the column jacket . In these preliminary experiments, 2M glucose
slightly buff ered at pH 8 .0 and containing a magnesium salt was used as
the substrate, and approximately 700 ml of isomerized syrup were collected
f per day .
~,
After 16 days, the enzyme was still active and approximately 45%
conversions of glucose t.o fr ;uctose were obtained . *The columns will be
run until exhaustion so that an approximate measure of enzyme stability
can be obtained . Although these are preliminary data, the potential of
this procedure is very significant . In comparing these data with a batch
enzymatic isomerization, the partially purified enzyme is being reused 16
times with potential for further reuse . Although the color units and ion
load were not determined, the % :olor of the isomerized syrup was comparable
to the initial syrup . The studies will be repeated using filtered high
DE syrups ; and variables such as pH, temperature and flow rates will be
examined .
Charles G . Pheil
CGP :gof