Blood Typing Lab

BIOLOGY 11
NAME: __________________________________________
Materials Needed per Group:
4 Blood Typing Slides
8 Toothpicks
1 Microscope Slide
Compound Microscope
Marker
Shared Materials:
Unknown Blood Samples from:
!
Mr. Smith
!
Mr. Jones
!
Mr. Green
!
Ms. Brown
Simulated
Anti-A Serum

Simulated Anti-B Serum

PROCEDURE:
1. Label each blood typing slide:
1. Mr. Smith
2. Mr. Jones
3. Mr. Green
4. Ms. Brown
2.
3.
4.
5.
6.

Place approximately 2 drops of Mr. Smiths blood in the A and B wells in Slide 1.
Place approximately 2 drops of Mr. Joness blood in the A and B wells in Slide 2.
Place approximately 2 drops of Mr. Greens blood in the A and B wells in Slide 3.
Place approximately 2 drops of Ms. Browns blood in the A and B wells in Slide 4.
Place approximately 2 drops of the simulated anti-A serum in each A well on the four
slides.
7. Place approximately 2 drops of the simulated anti-B serum in each B well on the
four slides.
8. Using a toothpick, stir each well for 30 seconds. Use a clean toothpick each time.
To avoid splattering the simulated blood, do not press too hard on the typing tray.
Place your slide on a piece of paper. This will make it easier to see the results.
9. Observe each slide and record your observations in Table 1 on the following page.
To confirm agglutination try reading text through the mixed sample. If you cannot
read the text, assume you have a positive agglutination reaction. In the Anti-A and
Anti-B columns of the table indicate either Agglutination or No Agglutination. In the
Blood Type column, predict the blood type of each individual based on the information
and your notes.

10. Clean up your station. After you find your results, you can wash the materials down
the sink using water and make sure you clean out the slide very well. Fill out the
Patient Report below and answer the questions. Please pass in this lab to be
marked.
Figure 1
Blood
Type

Antigens on
Erythrocytes
(Agglutinogens)

Antibodies in Plasma
(Agglutinins)

Can Give Blood

To

Can Receive
Blood From

Anti-B

A, AB

O, A

Anti-A

B, AB

O, B

AB

A and B

Neither Anti-A nor

Anti-B

AB

O, A, B, AB

Neither A nor B

Both Anti-A and Anti-B

O, A, B, AB

Table 1 - Results and Blood Types

Anti-A Serum

Anti-B Serum

Blood Type

Slide 1 - Mr. Smith

Slide 2 - Mr. Jones
Slide 3 - Mr. Green
Slide 4 - Ms.
Brown

Patient Report
1 a. Choose one of the following patients:
Mr. Smith
Mr. Jones
Mr. Green
Ms. Brown
1 b. Using the information shown in Figure 1 and the data recorded in Table 1, what
agglutinogens are present on the patients RBCs?
______________________________________________________________________
______________________________________________________________________

1 c. What ABO agglutinins are found in this patients plasma?

______________________________________________________________________
______________________________________________________________________
1 d. What is this patients blood type? _______________________________________
1 e. If this patient needed a blood transfusion, what blood type(s) could they safely
receive? ______________________________________________________________
1 f. What blood type(s) could this patient donate blood to?
______________________________________________________________________
2. Below is a diagram representing the blood type analysis of a new patient, Patient X.
From the information obtained from the slide, fill out the medical technologists report.

A
B
Medical Technologists Report
Patients Name:
ABO Type:
Med Tech Name (Your name):
3. List 3 situations where blood typing could be used.
______________________________________________________________________
______________________________________________________________________
______________________________________________________________________