Kajian Efektivitas Streptozotocin Dalam Induksi Tikus Putih Diabetik
Kajian Efektivitas Streptozotocin Dalam Induksi Tikus Putih Diabetik
Aldilanita Gelli Nita Ruskar, The Study Effectiveness of Streptozotocin To Induced Diabetes in Rats, This
thesis is guidenced by Dr. Dwi Winarni, Dra., M.Si and Drs. Saikhu Akhmad H , M. Kes; Department of
Biology, Science and Technology Faculty, Airlangga University, Surabaya
Abstract
This research has been purposed to know the effectiveness of streptozotocin to induced diabetes relied on
blood glucose level and diabetes group. To induce experimental diabetes in rat used diabetogenic agent
there are streptozotocin and nicotinamide. This research used 8 week rats with average body weight
110-280 gram as animal model. Introduction experiment had done before this reearch to optimizing doses
of STZ. variouse doses of STZ 60 mg/kg body weight, 75 mg/kg body weight, 100 mg/kg body weight and
125 mg/kg body weight. Each doses has different success level to induce diabetes in rats. From
introduction experiment showed that doses 100 mg/kg body weight STZ effective to induce diabetes in rats.
Type 2 diabetes in rats was induced by a single intra-peritoneal injection of streptozotocin (100 mg/ kg, i.p)
in citrate buffer pH 4,5, 15 min after the intra-peritoneal administration of Nicotinamide (240 mg/ kg, i.p)
in PBS. Fasting blood glucose was measured 7 days after injection STZ and nicotinamide. Rats were fasted
for 10-12 h before the test. Blood samples were taken from the tail then sucked with oxidase-peroxidase
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reactive strips, furthermore set it on glucometer (Accu-Check, Roche Diagnostic, USA) to see blood
glucose levels. Blood glucose level finding with mg/dl. Data in this research analyzed by t independent
sample.
Keywords : diabetes mellitus, streptozotocin, nicotinamide
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