Date

no.

(C)
Aim: To prepare and evaluate stable micro emulsion.
Formula:
Batch size:
Packaging:
Pack size:
Requirements: 50 ml beaker, pipette, dropper, measuring cylinder.
Formulation:
Ingredient

Quantity given Quantity

taken

Role of
ingredients

Oleic acid

8%

1.6 ml

Oil phase

Tween 80

72 %
(1:2)

9.6 ml

Surfactant

4.8 ml

Co-surfactant

20 %

4 ml

Water phase

PEG-400
Dist. Water

Method of preparation:
The given ingredients were taken as per their quantity.
First of all, surfactant (Tween 80) and co-surfactant (PEG-400) were mixed
using propeller stirrer.
Oleic acid was taken in an another 50 ml beaker.
Surfactant co-surfactant mixture was added in to oleic acid with
continuous stirring by propeller stirrer.
Then water was added drop wise in that mixture along with continuous
stirring.

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no.

Observation table :
1. Globule size: 40 nm.
2.Viscosity: 135 cps (20 rpm, 46.2 % torque)
3. pH: 6.12
4. Conductivity: 75 s.
5. Stability testing:

Condition

Time
duration

At 4c

For 24hr

At Room
temperature
By
centrifugation

For 24hr
For 30min
(1000RPM)

Observation

Interference

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no.

The trapped air bubbles were removed by stirring at low speed and keeping
it aside for few minutes.

Method of characterization:
1. Globule size:
2. Viscosity
3. Stability study:
Stability study was done at room temperature, at 4 C and by
centrifugation.
4. Conductivity:
It was measured using conductometer.
H
5. P :
The solution was prepared by taking 1 ml of microemulsion and
diluting it with 9 ml of distilled water.This solution was used to
measure the PH.

Comments:

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no.

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Reference:

no.