TOPO

CCCTT
GGGA

AGGG
TTCCC
TOPO

TK p
A

P CM

pU

i c i l li n

5.4 kb

N e o m y cin

40
SV

Am p

TM

pcDNA 3.3-TOPO

or

4
SV

TM

Comments for pcDNA 3.3-TOPO 5407 nucleotides

CMV promoter:
CMV forward primer binding site:

TOPO cloning site:

TK pA reverse primer binding site:
TK polyadenylation signal:
f1 replication origin:
SV40 early promoter:
Neomycin Resistance gene:
SV40 polyadenylation signal:
pUC origin (c):
Ampicillin (bla) resistance gene (c):
bla promoter (c):
(c) = complementary strand

47-726
584-604
741
787-805
780-1051
1087-1515
1520-1889
1925-2719
2895-3025
3408-4081
4226-5086
5087-5185