Anda di halaman 1dari 4

Stabilization of Enzyme using Transglutaminase

Etsushi CHAYA, Michio KITANO


Owari Textile Research Center, AITEC * 1

pH

TGase

100

2.1
H Bacillus

subtilis 900U/mg*1 H

Aspergillus niger 4U/mg*2N

3U/mgH Rhizopus sp. 8U/mg

TGase Streptoverticillium sp.

A 1U/mg*3*1

301 1g

1U

*2

401 1mol

1U *3

37pH=6.01 1mol

1U
2.2 TGase

pH=3.0, 4.0, 5.0, 6.0, 7.0,

pH

8.0, 9.0 TGase

20, 30, 40, 50, 60, 70

10, 20, 30, 40, 50, 60, 90, 120

2.3 TGase
TGase TGase

TGase SDSPAGE
15Bio-Rad

114.0

50.7

Bio-Rad BioSafe CBB G-250

35.5

30 10 TCA

M
1
210
320
440
560
6120

88.0

J 200V 50
2.4

M123456

(kDa)

28.8
22.0

660nm

40 5

2.5
TGase

TGase
-

TGase

TGase 304050

pH TGase TGase

556065707580 10

60

3.2 TGase

TGase
TGase

3.2.1
TGase
TGase

3.1 TGase
TGase -

TGase

-
(Glu)Lys

100

80


TGase

pH
TGase
SDS-PAGE

60

40

20

TGase SDS-PAGE

0
0

50

250

500

1000 2500

45UmU

TGase

TGase

TGase

6010

55

U TGase 520mU

60 70

TGase 510

pH 6.0

pH 7.0

100

pH 7.0 TGase

4050
TGase

60

80

60
40
20

100

20

40

80

60

80

60

10

40

60 5

20

10 60

0
3

10

2030
5060

TGase 2060
6010

TGase
70 10
1U TGase
15U pH

3.2.2

100
80
60
40
20

2030 pH 7.0

0
0

60

TGase
10
4

40

60

3.3 TGase
3.3.1 TGase

20

60

3.3.2 TGase

pH

TGase

70

60 10

TGase 60

50

TGase

TGase

1), 57, P125 (2001)

2)Usa Sangwatanaroj, Kingkamol Choonukulpong,

TGase

TGase

TGase

Mitsuo Ueda: AATCC REVIEW, 3, 17 (2003)


3)
69(10), 1301 (1995)
4)K. Ikura, T. Kometani, M. Yoshikawa, R. Sasaki
and H. Chiba: Agric. Biol. Chem., 44, 1567 (1980)
5)

6)