Tabel 1 Kurva standar TMP

[TMP] (M)

Absorbansi terukur

Absorbansi terkoreksi

Blanko
2,5
5,0
7,5
10,0
15,0
30,0

0,202
1,018
0,282
1,677
0,463
2,559
1,111

0,816
0,080
1,475
0,261
2,357
0,909

Contoh perhitungan:
Absorbansi terkoreksi = Absorbansi terukur Absobansi blanko
= 1,018 0,202
= 0,816
Gambar 1 Kurva standar TMP
2.5
2
1.5
Absorbansi

1
0.5
0
0

f(x) = 0.02x + 0.75

R = 0.06
5

10

15

20

25

30

35

[TMP] (M)

Tabel 2 Analisis lipid peroksida

Sampel

Absorbansi teruku

Absorbansi terkoreksi

[MDA] (mg/dL)

Blanko
Normal
Kontrol positif1
Kontrol positif2
PTU1
PTU2
PTU3
PTU + Ekstrak

0,000
0,650
0,763
0,899
0,759
0,174
0,135
1,129

0,650
0,763
0,899
0,759
0,174
0,135
1,129

-3.5184
0.4900
5.3901
0.3459
-20.7317
-22.1368
8.6472

Contoh perhitungan (kontrol positif 2):

Absorbansi terkoreksi = Absorbansi terbaca Absorbansi blanko
= 0.899 0,000
= 0.899
y
= 0,02 x + 0,7494
0.889 = 0,02 [MDA] + 0,7494
[MDA]
= 6.98 M

= 6.98 M x

10 M
M

= 6.98 x 10-6 M
6.98 x 10-6 M

6.98 x 10-6 M

-6

6.98 x 10 M
-6

6.98 x 10 M

g
x
Mr MDA !
1 mg 1 L
+ 3+
L
10 10 dL

g
x
72.06 !
1 mg 1 L
+ 3+
L
10 10 dL

mg
72.06 x 104 dL

= 5.3901

mg
dL