0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
6
6.5
7
7.5
8
8.5
9
9.5
10
10.5
11
11.5
12
12.5
13
13.5
14
14.5
15
15.5
16
16.5
17
17.5
18
18.5
19
19.5
20
t (seconds)
0
30
60
90
120
150
180
210
240
270
300
330
360
390
420
450
480
510
540
570
600
630
660
690
720
750
780
810
840
870
900
930
960
990
1020
1050
1080
1110
1140
1170
1200
A
0.588
0.579
0.579
0.579
0.574
0.574
0.574
0.567
0.573
0.573
0.564
0.565
0.566
0.566
0.562
0.562
0.562
0.562
0.562
0.562
0.561
0.561
0.561
0.560
0.560
0.560
0.559
0.559
0.558
0.558
0.557
0.557
0.556
0.555
0.554
0.554
0.553
0.553
0.552
0.552
0.551
ln A
-0.5310
-0.5465
-0.5465
-0.5465
-0.5551
-0.5551
-0.5551
-0.5674
-0.5569
-0.5569
-0.5727
-0.5709
-0.5692
-0.5692
-0.5763
-0.5763
-0.5763
-0.5763
-0.5763
-0.5763
-0.5780
-0.5780
-0.5780
-0.5798
-0.5798
-0.5798
-0.5816
-0.5816
-0.5834
-0.5834
-0.5852
-0.5852
-0.5870
-0.5888
-0.5906
-0.5906
-0.5924
-0.5924
-0.5942
-0.5942
-0.5960
1/A
1.701
1.727
1.727
1.727
1.742
1.742
1.742
1.764
1.745
1.745
1.773
1.770
1.767
1.767
1.779
1.779
1.779
1.779
1.779
1.779
1.783
1.783
1.783
1.786
1.786
1.786
1.789
1.789
1.792
1.792
1.795
1.795
1.799
1.802
1.805
1.805
1.808
1.808
1.812
1.812
1.815
1.840
1.820
1.800
1.780
1.760
1/Absorbance
1.740
1.720
1.700
1.680
1.660
1.640
0
200
400
600
Time (seconds)
800
0.600
0.590
0.580
0.570
Absorbance
0.560
0.550
0.540
0.530
0
200
400
600
800
Time (seconds)
-0.4800
0
200
400
600
-0.5000
-0.5200
-0.5400
ln Absorbance
-0.5600
-0.5800
-0.6000
-0.6200
Time (seconds)
800
s)
-0.6000
-0.6200
Time (seconds)
800
1000
1200
800
1000
1200
800
1000
1200
ds)
s)
s)
t (minutes)
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
6
6.5
7
7.5
8
8.5
9
9.5
10
10.5
11
11.5
12
12.5
13
13.5
14
14.5
15
15.5
16
16.5
17
17.5
18
18.5
19
19.5
20
t (seconds)
0
30
60
90
120
150
180
210
240
270
300
330
360
390
420
450
480
510
540
570
600
630
660
690
720
750
780
810
840
870
900
930
960
990
1020
1050
1080
1110
1140
1170
1200
A
0.292
0.248
0.219
0.197
0.180
0.164
0.151
0.140
0.130
0.122
0.113
0.109
0.103
0.098
0.093
0.088
0.083
0.083
0.077
0.076
0.071
0.071
0.070
0.069
0.065
0.064
0.063
0.057
0.057
0.056
0.055
0.050
0.050
0.049
0.048
0.048
0.047
0.046
0.041
0.041
0.041
ln A
-1.231
-1.394
-1.519
-1.625
-1.715
-1.808
-1.890
-1.966
-2.040
-2.104
-2.180
-2.216
-2.273
-2.323
-2.375
-2.430
-2.489
-2.489
-2.564
-2.577
-2.645
-2.645
-2.659
-2.674
-2.733
-2.749
-2.765
-2.865
-2.865
-2.882
-2.900
-2.996
-2.996
-3.016
-3.037
-3.037
-3.058
-3.079
-3.194
-3.194
-3.194
1/A
3.425
4.032
4.566
5.076
5.556
6.098
6.623
7.143
7.692
8.197
8.850
9.174
9.709
10.204
10.753
11.364
12.048
12.048
12.987
13.158
14.085
14.085
14.286
14.493
15.385
15.625
15.873
17.544
17.544
17.857
18.182
20.000
20.000
20.408
20.833
20.833
21.277
21.739
24.390
24.390
24.390
30.000
25.000
R = 0.9944232963
20.000
1/Absorbance
15.000
10.000
5.000
0.000
0
200
400
600
Time (seconds)
800
0.350
0.300
0.250
0.200
Absorbance
R = 0.7626337846
0.150
0.100
0.050
0.000
0
200
400
600
800
800
Time (seconds)
0.000
0
200
400
600
-0.500
-1.000
-1.500
ln Absorbance
R = 0.9413861525
-2.000
-2.500
-3.000
-3.500
Time (seconds)
s)
-3.000
-3.500
Time (seconds)
800
1000
1200
800
1000
1200
800
1000
1200
ds)
s)
s)
t (minutes)
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
6
6.5
7
7.5
8
8.5
9
9.5
10
10.5
11
11.5
12
12.5
13
13.5
14
14.5
15
15.5
16
16.5
17
17.5
18
18.5
19
19.5
20
t (seconds)
0
30
60
90
120
150
180
210
240
270
300
330
360
390
420
450
480
510
540
570
600
630
660
690
720
750
780
810
840
870
900
930
960
990
1020
1050
1080
1110
1140
1170
1200
A
0.152
0.117
0.094
0.075
0.060
0.048
0.039
0.029
0.022
0.016
0.010
0.009
0.003
-0.002
-0.003
0.562
0.562
0.562
0.562
0.562
0.561
0.561
0.561
0.560
0.560
0.560
0.559
0.559
0.558
0.558
0.557
0.557
0.556
0.555
0.554
0.554
0.553
0.553
0.552
0.552
0.551
ln A
-1.8839
-2.1456
-2.3645
-2.5903
-2.8134
-3.0366
-3.2442
-3.5405
-3.8167
-4.1352
-4.6052
-4.7105
-5.8091
Err:502
Err:502
-0.5763
-0.5763
-0.5763
-0.5763
-0.5763
-0.5780
-0.5780
-0.5780
-0.5798
-0.5798
-0.5798
-0.5816
-0.5816
-0.5834
-0.5834
-0.5852
-0.5852
-0.5870
-0.5888
-0.5906
-0.5906
-0.5924
-0.5924
-0.5942
-0.5942
-0.5960
1/A
6.579
8.547
10.638
13.333
16.667
20.833
25.641
34.483
45.455
62.500
100.000
111.111
333.333
-500.000
-333.333
1.779
1.779
1.779
1.779
1.779
1.783
1.783
1.783
1.786
1.786
1.786
1.789
1.789
1.792
1.792
1.795
1.795
1.799
1.802
1.805
1.805
1.808
1.808
1.812
1.812
1.815
350.000
300.000
250.000
200.000
1/Absorbance
150.000
100.000
50.000
0.000
0
50
100
150
200
Time (seconds)
250
300
0.160
0.140
0.120
0.100
Absorbance
0.080
0.060
0.040
0.020
0.000
0
50
100
150
200
250
300
250
300
Time (seconds)
0.0000
0
50
100
150
200
-1.0000
-2.0000
-3.0000
ln Absorbance
-4.0000
-5.0000
-6.0000
-7.0000
Time (seconds)
s)
-6.0000
-7.0000
Time (seconds)
250
300
350
400
250
300
350
400
250
300
350
400
ds)
s)
s)