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Department of Biochemical Engineering and BioteclmQlogy
Minor I
BioinformaticsBEL418
MM-20
Time 1 Hr

1. The central dogma suggests DNA-R.NA-ProteL>1-r.mct;on.However, when deciphering human genome


information and annotation, many a times this dogma dcesn't work. Give possible reasons for the same.(4)
2. The end of human l3-globin gene has the nucleotide sequence:
~ '
. . . ctg gcc cag aag tat cac taa '~~

Writea)theAnucleotide sequence of single/multiple base change (minimum in case of multiple) producing


silept mutation. ~
b) A missence mutation. t?;7~.
c) Mutation leading to premature chain termination ~'" '&~A
d) Improper chain termination resulting in extended polypeptide chain
e) Change in one amino acid to a similar amino acid ~~ ~f~V}.
t) Minimum change that will possibly help disulphide bond formation.

3. The .following protein


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is human alpha chain haemoglobin


~ttr4
A~~
VLSP ADKTNV KAA WGKVGAH AGEYGAEALE RMFLSFPITK TYFPHFDLSH r'~,
GSAQVKGHGK KV ADAL TNA V AHVDDMPNAL SALSDLAHK LRVDPNKKL
LSHCLL VTLA AHLP AEFTPA VHASLDKFLA SVSTVL TSKY R ~4,~~
a) Identifyany (3) ~.~~
Trypsincleavagesites by indicatingthe resultingpeptideftagments 1<.) l(
Cyanogensbromidec!~a\jage ~~ ht1fl.
Chymotrypsincleavagesites Slt~S -rY' I "'kp IT ~ / (V\4 ~ f2) 'I I 1AJ )
L)tV)

b) Once you ge,t the pep:ides, how will ~.ougo about getting the sequence ofthe fUtire protein.(4)

4. Protein structural features are always fascinating. Based on their classifications write in short about the
following
SCOP, CATH databases
and FSSP (6)
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