Nama

: Herlani Tri Widhiastuti

NIM

: G84120046
Analisis ProtParam Mycobacterium tuberculosis

User-provided sequence:
10
20
30
40
50
60
GSHMTPPHNY LAVIKVVGIG GGGVNAVNRM IEQGLKGVEF IAINTDAQAL LMSDADVKLD
70
80
90
100
110
120
VGRDSTRGLG AGADPEVGRK AAEDAKDEIE ELLRGADMVF VTAGEGGGTG TGGAPVVASI
130
140
150
160
170
180
ARKLGALTVG VVTRPFSFEG KRRSNQAENG IAALRESCDT LIVIPNDRLL QMGDAAVSLM
190
200
210
220
230
240
DAFRSADEVL LNGVQGITDL ITTPGLINVD FADVKGIMSG AGTALMGIGS ARGEGRSLKA
250
260
270
280
290
300
AEIAINSPLL EASMEGAQGV LMSIAGGSDL GLFEINEAAS LVQDAAHPDA NIIFGTVIDD
310
320
330
340
350
360
SLGDEVRVTV IAAGFDVSGP GRKPVMGETG GAHRIESAKA GKLTSTLFEP VDAVSVPLHT
370
380
NGATLSIGGD DDDVDVPPFM RR

Number of amino acids: 382

Molecular weight: 39037.1
Theoretical pI: 4.58
Amino acid composition:
CSV format

Ala
Arg
Asn
Asp
Cys
Gln
Glu
Gly
His
Ile

(A)
(R)
(N)
(D)
(C)
(Q)
(E)
(G)
(H)
(I)

46
19
13
30
1
7
22
53
5
25

12.0%
5.0%
3.4%
7.9%
0.3%
1.8%
5.8%
13.9%
1.3%
6.5%

Leu
Lys
Met
Phe
Pro
Ser
Thr
Trp
Tyr
Val
Pyl
Sec
(B)
(Z)
(X)

(L)
(K)
(M)
(F)
(P)
(S)
(T)
(W)
(Y)
(V)
(O)
(U)

32
12
12
11
15
23
20
0
1
35
0
0

8.4%
3.1%
3.1%
2.9%
3.9%
6.0%
5.2%
0.0%
0.3%
9.2%
0.0%
0.0%

0
0
0

0.0%
0.0%
0.0%

Total number of negatively charged residues (Asp + Glu): 52

Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon
Hydrogen
Nitrogen
Oxygen
Sulfur

C
H
N
O
S

1689
2759
481
551
13

Formula: C1689H2759N481O551S13
Total number of atoms: 5493
Extinction coefficients:
This protein does not contain any Trp residues. Experience
shows that
this could result in more than 10% error in the computed
extinction coefficient.
Extinction coefficients are in units of
measured in water.

M-1 cm-1, at 280 nm

Ext. coefficient
1490
Abs 0.1% (=1 g/l)
0.038, assuming all pairs of Cys
residues form cystines
Ext. coefficient
Abs 0.1% (=1 g/l)
reduced
Estimated half-life:

1490
0.038, assuming all Cys residues are

The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian
reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in
vivo).
Instability index:
The instability index (II) is computed to be 29.12
This classifies the protein as stable.

Aliphatic index: 96.81

Grand average of hydropathicity (GRAVY): 0.107

Simpulan
Berdasarkan analisis ProtParam yang telah dilakukan, Mycobacterium
tuberculosis mengandung asam amino glisin dan alanin paling banyak yaitu
sebesar 13.9% dan 12%.