BOOK
FORENSIC LABORATORY
Presented by:
Dra.Suhartini Apt, SU
Dr. IdhaArfianti, Msc
Forensic Department
Gadjah Mada University
2014
Trace evidence are commonly found in the crime scenes and in the body of the victims or
assailants. Therefore, we can find some biological material such as; blood, semen, saliva, and
urine. In this examination, we can find the semen using Woods Lamp.
B. Equipment
1. Woods Lamp
2. Fabrics with a biologic material
3. Gloves
4. Scissors
5. Envelope
C. Procedure
1. Observe the fabrics with Woods lamp.
2. Determine the suspected semen.
3. Cut the suspected site.
4. Insert to the envelope to further examination.
D. Result
BLOOD IDENTIFICATION
peroxide. Blood contains an enzyme called catalase, which breaks down hydrogen peroxide
into water and oxygen gas:
2H2O2---------- 2H2O + O2
Catalase
When this reaction occurs, the oxygen gas is released as bubbles. These substances that
bubble with the addition of hydrogen peroxide are said to test positive for catalase.
B. Equipment
1. Hydrogen Peroxide Spray
2. Fabrics with suspected blood
3. Gloves
C. Procedure
1. Spray the fabrics with hydrogen peroxide
2. Observe the positive result (Foamy Appearance)
D. Picture
B. Equipment
1. Fabrics with semen like material
2. Sterile cotton tip
3. Object glass and cover glass
4. Pippete
5. Reaction tube
6. Microscope
7. Spermin Stain
8. Wrigth Stain
9. Phosphate buffer
10. Gloves
C. Procedure
1. Wet slide
a. Smear the sample in to the object glass.
b. Add NaCl.
c. Give the cover glass.
d. Observe the sperm under the microscope.
2. Dry slide
a. Smear the sample in to the object glass.
b. Let it dry in the room temperature.
c. Give the cover glass.
d. Observe the sperm under the microscope.
3.Wright Staining
a. Smear the sample in to the object glass.
b. Add Wright Stain until it is soaked.
c. Add Phosphate Buffer until soaked (for about 25 minutes).
d. Wash the stain with water.
e. Give the cover glass.
f. Observe the sperm under the microscope.
4.Spermin Staining
a. Smear the sample in to the object glass.
b. Add Spermin Stain until it is soaked (for about 15 minutes).
d. Discharge the fluid.
e. Give the cover glass.
f. Observe the spermin crystal under the microscope.
D. Picture
(10x Sperma)
(40x Sperma)
(10x Spermin)
(40x Spermin)