Presenter: M. S. Rahman
Moderator: Dr. Varsha A. Singh
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Introduction
History
1975- First identified by Appleton, Higgins,
Madeley and Cosgrove
1982- Caul and Appleton visualized astrovirus
using appropriate immunological reagents
Eight traditional serotypes have been identified
in humans
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Epidemiology
Under 5 years old -9.9% of the 6.9 million
deaths (2011)
Mexico City- 365 diarrhoea episodes among 214
children (18 months old)
Brazil- 43 cases in 2004.
UK - in a primary immunodeficiency unit at
Newcastle General Hospital
China- 335 infants with diarrhoea in Wuhan
Spain- 4.9 % in children.
Korea -10,603 stool samples ( 2004 to 2008) 1.8 %
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Classification
Family- Astroviridae
Two genera-
1.Mamoastrovirus(MAstVs)
2.Avastrovirus(AAstVs)
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Mamalian Ast V
Include
Bovine AstV
Feline AStV
Human
AstV(Genotype 1-8)
Ovine AstV
Mink AstV
Porcine AstV
Avian Astrovirus
include
Duck AstV1
Turky Astv1 &2
Avian Nephritis
Virus(ANV)
Classification based on
the host of origin
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Capsid
Multidomain protein
S Domain
P1 Domain
P2 Domain
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Spikes
Acidic
Domain
Genome Structure
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Viral Genome
ORF1a
Viral nonstructural
proteins & VPg
ORF1b
RNA-dependent
RNA polymerase
ORF2
Capsid
protein
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Mode of Transmission
Fecaloral route
Person-to-person contact.
Vomit and environmental surfaces (such as
hand-mouth contact)
Consumption of shellfish (faecally
contaminated)
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C1q/MBL
Seasonal Variation
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Lab Diagnosis
Sample: Feaces
Electron microscopy
Enzyme immunoassays
Astrovirus Capsid ELISA
Reverse transcription-polymerase chain
reaction (RT-PCR)
Nucleic acid dot-blot hybridization test
One step test
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Lab diagnosis
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NO VACCINE
In the future, immunization may play an
important role for preventing HAstV
infections.
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Thank You
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