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Vierling 1

Sarah Vierling
Mr. Jackson
Biology P1
16 November 2015
Enzyme Lab Report
I.
II.

Purpose: What is the difference between a catalyst and an enzyme?


Hypothesis: If substances containing enzymes are placed in similar environments as a
catalyst, then the differences may be determined.

III.

Experiment/Procedure: See procedure sheet.

IV.

Data & Observations: See Enzyme Lab Data Sheet.

V.

Conclusion:
The purpose of this experiment was to discover the difference between a catalyst and an

enzyme. The class decided that if substances containing enzymes are placed in similar
environments as a catalyst, then the differences may be determined.
In the lab, our group combined H2O2 with liver and potato (enzymes), sand (nonreactive), and MnO2 (catalyst) separately and combinations, also testing the effects of heat and
grinding. The liver, MnO2, and potato all made a reaction. The heat reduced the rate of reaction
for the liver and MnO2. In experiment 7, my group filled a test tube with 2 ml of H2O2 and
placed a small cube of liver and a sprinkle of sand in a mortar. We ground the substances in the
mortar and then poured them into the test tube filled with H2O2. On a scale of 0 - 3 of reaction,
the group rated it a 3. In experiment 6, H2O2 and liver were mixed and created the same
reaction: 3. Therefore, the group concluded the liver contained enzymes which reacted with the
H2O2, while the sand did not act as a catalyst.

Vierling 2
In experiment 4, the group added a small amount of MnO2 to 2 ml of H2O2, observing a
reaction rate of 2. In experiment 9, the group put 2 ml of H2O2 and a small amount of MnO2 in
a test tube. The test tube was placed into a boiling water bath for 5 minutes. The group then
proceeded to remove the tube from the bath and add 2 ml of H2O2. The rate of reaction was 1.
The group decided the MnO2 acted as a catalyst, though the rate of reaction was lower with heat
and lower in both experiments than the rates observed with liver. Experiment 11 added potato to
H202, producing a mild reaction of 1.
The experiments involving enzymes (liver and potato) and catalyst (MnO2) all showed
reactions with H2O2. The liver reacted most strongly, but the potato, also an enzyme was weak.
MnO2 also produced a weak reaction. Errors are possible in measuring the quantities of
materials added to the H2O2, mistakes in timing, and the subjective observation of the rate of
reaction. Our group did not test different amounts of material added to H2O2, which could affect
the rate of reaction.
The control was water, and none of the materials added to water caused a reaction (as
expected). The manipulated variables were the materials that were added to the H2O2 or water
and the addition of heat and mortar grinding. The responding variable was the rate of reaction
(how high the bubbles rose). Based upon the results, both enzymes and catalysts caused reactions
with H2O2. The rate of reaction was also affected by heat. Sand did not react at all with H2O2.
The hypothesis was not confirmed by the Enzyme vs. Catalyst Lab. The reactions of
enzymes and catalysts with H2O2 were similar, and our group could not draw definite
conclusions about the differences between enzymes and catalysts. Instead, the definition of
enzymes and catalysts provides the difference between them. Enzymes are biological catalysts.

Vierling 3
Catalysts though do not have to be enzymes. Catalysts can include non-biological substances
such as chemicals.
Our class learned that enzymes and catalysts behave similarly when combined with reactive
substances.

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