49

LAMPIRAN - LAMPIRAN

50

Lampiran I. Surat Keterangan Serbuk Daun Jarak Pagar (Jatropha curcas Linn).

51

Lampiran II. Surat Persetujuan Etik (Ethical Approval) untuk penelitian jarak pagar

52

Lampiran III. Data Perhitungan Rendeman Berdasarkan Ekstak Kental Etanol Daun
Jarak Pagar

Ekstrak Etanol
Bobot ekstrak etanol
Rendemen

= 53,3 gram
53,3
= 1000 x 100 %

= 5,33 %
Fraksi tidak larut n-heksan
Bobot fraksi
= ekstrak kental etanol fraksi tidak larut n-heksan
= 53,3 gram 7,87 gram
= 45,43 gram
45,43
Rendemen
= 53,3 x 100 %
= 85,23 %

Fraksi larut Kloroform

Bobot fraksi
Rendemen

= 3,35 gram
3,35
= 53,3 x 100 %
= 6,29 %

53

Lampiran IV. Data hasil uji antiinflamasi fraksi klorofrom daun J.curcas pada kaki
tikus wistar yang diinduksi karagenan 1 %

Tabel I. Data volume edema setiap 1 jam selama 6 jam pengamatan

Waktu
(jam)
Vo

1
1
1,5

1
2

1,5
1,7
1,8

3
4
5

1,5
1,25

6
Waktu
(jam)
Vo

1
1

1
2
3

1,175
1,225
1,3

4
1,3
5
1,1
6

Waktu
(jam)
Vo
1
2
3
4
5
6

1
0,975
1,2
1,25
1,35
1,3
1,25
1,1

1,1

Kelompok I (ml)
2
3
0,92
5
0,8
1,1
1,15
1,32
5
1,175
1,4
1,2
1,7
1,225
1,52
5
1,2
1,2
1,1
Kelompok III (ml)
2
3
1,02
5
1
1,17
5
1,15
1,2
1,25
1,22
5
1,3
1,27
5
1,3
1,47
5
1,1
1,35

Kelompok V (ml)
2
3
1,05
1,05
1,2
1,2
1,225 1,325
1,375 1,425
1,45 1,275
1,35 1,175
1,225 1,075

1,05

4
1,175
1,35
1,45
1,6
1,75
1,575
1,45

Kelompok II (ml)
2
3

0,8
1,15

0,65

1,25

1,275
1,25
1,325

1,15

1
1,15

1
1,05

1,25
1,,25
1,2

0,95
0,85
0,85

1,2
1,175
1,1

1,2
1,15
1,05

1,05
1,05

1,15
1

0,75
0,8

1,075
1

1,05
1

1,2

1,1

0,9

1,3
1,55

1,375
1,55

1,3
1,45

1,375
1,4

1,3
1,5

1,65

1,65

1,45

1,35

1,35

1,575

1,55

1,45

1,25

1,425

1,6

1,4

1,25

1,275

1,5

1,3

1,5
1,37
5
1,37
5
1,32
5

Kelompok IV (ml)
2
3

Keterangan :
Kelompok 1 = kontrol negatif
Kelompok 2 = kontrol positif
Kelompok 3 = FKDJP 150 mg
Kelompok 4 = FKDJP 300 mg
Kelompok 5 = FKDJP 600 mg

54

55

Tabel 2. Rata-rata presentase kenaikan volum edema pada kelompok kontrol dan
kelompok perlakuan fraksi kloroform daun J.curcas setiap 1 jam selama 6 jam
pengamatan

Waktu
(Jam ke-)
1
2
3
4
5
6

Rata-rata Kenaikan Volume Edem (%) SE

Kelompok
I
II
III
IV
39,10 6,89
30,48 16
13,87 1,95
34,62 7,24
49,87 3,45
27,79 6,23
23,43 2,53
48,09 5,57
56,90 4,56
22,07 3,59
29,25 3,69
49,20 6,79
70,63 7,02
16,44 5,7
24,22 4,12
49,25 5,89
49,03 6,88
10,72 2,63
17,02 9,06
45,25 3,80
30,87 2,58
5,77 5,76
12,72 6,46
35,20 3,57

V
16,63 2,15
23,62 2,51
35,32 1,58
35,45 5,69
25,68 4,78
13,81 4,39

Tabel 3. Data Area Under Curve/ AUC0-6 (% jam) persentase kenaikan volum edema
terhadap waktu setiap 1 jam selama 6 jam pengamatan

Waktu
(jam)
Vo
1
2
3
4
5
6
kumulati
f

1
0
25
50
60
75
65
37,5
312,5

Purata
SE
Waktu
(jam)
Vo
1
2
3
4
5
6
kumulati
f

Kelompok II (ml)
1
2
3
0
0
0
12,5
38,46
7,5
25
61,54
17,5
25
38,46 18,75
22,5
30,77 13,75
17,5
23,08 8,75
7,5
19,23
3,75
110

280,975 10,93

1
0
8,75
20
30
30
20
10

118,75
Purata

Kelompok I (ml)
2
3
4
0
0
0
9,46
21,87
21,87
31,08 45,31
51,56
47,29 48,43
57,81
67,57 51,56
60,93
74,32 51,56
48,43
47,29 43,75
31,25
277,0
271,87
262,5
2
5

70

50

110,39 35,95

Kelompok III (ml)

2
3
4
0
0
0
7,32
7,5
4,167
34,15
20
18,75
18,29 27,5
33,33
21,95
30
25
34,15
20
8,33
37,80
7,5
4,17

153,66 112,5
119,66 12,51

211,54

4
0
2,5
12,5
17,5
10
5
25

1
0
18,75
46,25
60
61,25
50
35
271,2
5

Kelompok IV (ml)
2
3
0
0
9,09
26,39
25
54,17
31,82
52,78
36,36
55,56
43,18
58,33
40,90
50
186,36

297,22

93,75
243,39 25,05

4
0
15
40
50
43,75
37,5
32,5
218,75

56

SE

Waktu
(jam)
Vo
1
2
3
4
5
6
kumulat
if
Purata
SE

1
0
11,54
28,20
33,33
35,89
30,77
20,51
160,25
6

Kelompok V (ml)
2
3
4
0
0
0
7,14
7,14
7,45
15,48
20,24
19,15
23,81
30,95
29,79
34,52
28,57
42,55
33,33
16,67
41,49
22,62
7,14
28,72
136,90 110,71 169,14
5
4
9
144,256 13,09

Tabel 4. Data daya antiinflamasi (%) fraksi kloroform daun J.curcas dan kontrol positif
(natrium diklofenak 4,5 mg/kg BB)
Tikus
1
2
3
4
Purata SE

Kelompok II
60,85
24,71
75,09
82,20
60,71 12,79

Daya Antiinflamasi (%)

Kelompok III
Kelompok IV
57,74
3,46
45,31
33,67
59,96
-5,78
66,63
22,15
57,41 4,45
13,37 8,92

Kelompok V
42,96
51,28
60,59
39,79
48,65 4,66

57

Lampiran V. Hasil analisis statistik AUC dari hubungan persentase kenaikan volum
edema terhadap waktu pada uji efek antiinflamasi fraksi kloroform
daun J. curcas pada kaki tikus Wistar yang di induksi karagenan 1%

NPar Tests
One-Sample Kolmogorov-Smirnov Test
NILAI AUC
N
Normal Parametersa,b

Most Extreme Differences

20
Mean

179,7350

Std. Deviation

80,89203

Absolute

,147

Positive

,125

Negative

-,147

Kolmogorov-Smirnov Z

,657

Asymp. Sig. (2-tailed)

,781
,732c

Sig.
Monte Carlo Sig. (2-tailed)

95% Confidence Interval

Lower Bound

,723

Upper Bound

,740

a. Test distribution is Normal.

b. Calculated from data.
c. Based on 10000 sampled tables with starting seed 299883525.

58

Oneway

Descriptives
NILAI AUC
N

Mean

KONTROL
KONTROL

FKDJP 150

FKDJP 300

FKDJP 600

Total

95% Confidence Interval

Minimu

Maximu

Deviation

Error

for Mean

21,85976

5
110,384

POSITIF

Std.

280,975

NEGATIF

Std.

71,90126

5
119,665

25,02946

0
243,395

50,11121

0
144,255

26,17431

0
179,735

20

80,89203

10,9298
8
35,9506
3
12,5147
3
25,0556
0
13,0871
6
18,0880
1

Lower

Upper

Bound

Bound

246,1917

315,7593

262,50

312,50

-4,0265

224,7955

50,00

211,54

79,8375

159,4925

93,75

153,66

163,6569

323,1331

186,36

297,22

102,6058

185,9042

110,71

169,15

141,8764

217,5936

50,00

312,50

Test of Homogeneity of Variances

NILAI AUC
Levene Statistic
1,948

df1

df2
4

Sig.
15

,155

ANOVA
NILAI AUC
Sum of Squares

df

Mean Square

Between Groups

95915,852

23978,963

Within Groups

28411,027

15

1894,068

124326,878

19

Total

F
12,660

Sig.
,000

59

Post Hoc Tests

Multiple Comparisons
Dependent Variable: NILAI AUC
LSD
(I) KELOMPOK

(J) KELOMPOK

Mean

Std. Error

Sig.

Difference (I-J)

KONTROL NEGATIF

FKDJP 300

170,59101

30,77392

,000

104,9979

236,1841

FKDJP 150

161,31051*

30,77392

,000

95,7174

226,9036

FKDJP 300

37,58051

30,77392

,241

-28,0126

103,1736

FKDJP 600

136,72051*

30,77392

,000

71,1274

202,3136

-170,59101

30,77392

,000

-236,1841

-104,9979

FKDJP 150

-9,28050

30,77392

,767

-74,8736

56,3126

FKDJP 300

-133,01050*

30,77392

,001

-198,6036

-67,4174

FKDJP 600

-33,87050

30,77392

,288

-99,4636

31,7226

-161,31051

30,77392

,000

-226,9036

-95,7174

9,28050

30,77392

,767

-56,3126

74,8736

FKDJP 300

-123,73000

30,77392

,001

-189,3231

-58,1369

FKDJP 600

-24,59000

30,77392

,437

-90,1831

41,0031

KONTROL NEGATIF

-37,58051

30,77392

,241

-103,1736

28,0126

30,77392

,001

67,4174

198,6036

30,77392

,001

58,1369

189,3231

30,77392

,006

33,5469

164,7331

-136,72051

30,77392

,000

-202,3136

-71,1274

KONTROL POSITIF

33,87050

30,77392

,288

-31,7226

99,4636

FKDJP 150

24,59000

30,77392

,437

-41,0031

90,1831

FKDJP 300

30,77392

,006

-164,7331

-33,5469

KONTROL POSITIF

KONTROL POSITIF
FKDJP 150
FKDJP 600
KONTROL NEGATIF

FKDJP 600

Upper Bound

KONTROL POSITIF

KONTROL NEGATIF
FKDJP 150

Lower Bound

KONTROL NEGATIF
KONTROL POSITIF

95% Confidence Interval

133,01050
123,73000
99,14000

-99,14000

*. The mean difference is significant at the 0.05 level.

60

Lampiran VI. Hasil analisis statistic DAI pada uji efek antiinflamasi fraksi kloroform
daun J. curcas pada kaki tikus wistar yang di induksi karagenan 1%

NPar Tests
One-Sample Kolmogorov-Smirnov Test
PERSENTASE
DAYA
ANTIINFLAMASI
N
Normal Parametersa,b

Most Extreme Differences

16
Mean

45,0381

Std. Deviation

24,59628

Absolute

,135

Positive

,080

Negative

-,135

Kolmogorov-Smirnov Z

,539

Asymp. Sig. (2-tailed)

,934
,902c

Sig.
Monte Carlo Sig. (2-tailed)

95% Confidence Interval

Lower Bound

,896

Upper Bound

,907

a. Test distribution is Normal.

b. Calculated from data.
c. Based on 10000 sampled tables with starting seed 926214481.

61

Oneway
Descriptives
PERSENTASE DAYA ANTIINFLAMASI
N

Mean

Std.

Std.

Deviation

Error

95% Confidence Interval for Minimum Maximum

Mean
Lower Bound Upper Bound

KONTROL

60,7125

FKDJP 150

57,4100

8,90748

FKDJP 300

13,3750

FKDJP 600

4
16

POSITIF

Total

25,59052 12,79526

19,9923

101,4327

24,71

82,20

4,45374

43,2362

71,5838

45,31

66,63

17,83357

8,91679

-15,0022

41,7522

-5,78

33,67

48,6550

9,31587

4,65793

33,8314

63,4786

39,79

60,59

45,0381

24,59628

6,14907

31,9317

58,1446

-5,78

82,20

Test of Homogeneity of Variances

PERSENTASE DAYA ANTIINFLAMASI
Levene Statistic
1,726

df1

df2
3

Sig.
12

,215

ANOVA
PERSENTASE DAYA ANTIINFLAMASI
Sum of Squares

df

Mean Square

Between Groups

5657,538

1885,846

Within Groups

3417,119

12

284,760

Total

9074,657

15

F
6,623

Sig.
,007

62

Post Hoc Tests

Multiple Comparisons
Dependent Variable: PERSENTASE DAYA ANTIINFLAMASI
LSD
(I) KELOMPOK

(J) KELOMPOK

Mean

Std. Error

Sig.

Difference (I-J)

95% Confidence Interval

Lower Bound

Upper Bound

FKDJP 150

3,30250

11,93231

,787

-22,6958

29,3008

KONTROL POSITIF FKDJP 300

47,33750

11,93231

,002

21,3392

73,3358

FKDJP 600

12,05750

11,93231

,332

-13,9408

38,0558

KONTROL POSITIF

-3,30250

11,93231

,787

-29,3008

22,6958

FKDJP 300

44,03500

11,93231

,003

18,0367

70,0333

FKDJP 600

8,75500

11,93231

,477

-17,2433

34,7533

11,93231

,002

-73,3358

-21,3392

11,93231

,003

-70,0333

-18,0367

FKDJP 600

-35,28000

11,93231

,012

-61,2783

-9,2817

KONTROL POSITIF

-12,05750

11,93231

,332

-38,0558

13,9408

FKDJP 150

-8,75500

11,93231

,477

-34,7533

17,2433

FKDJP 300

35,28000*

11,93231

,012

9,2817

61,2783

FKDJP 150

KONTROL POSITIF
FKDJP 300

FKDJP 600

FKDJP 150

-47,33750
-44,03500

*. The mean difference is significant at the 0.05 level.

63

Tikus

Jumlah Neutrofil ( /100 sel leukosit)

Kelompok
I

Kelompok
II

Kelompok
III

Kelompok
IV

Kelompok
V

14

12

II

11

13

III

12

Purata

12,33

2,67

10,67

Lampiran VII. Data hasil pengamatan histopatologi jumlah neutrofil per 100 sel
leukosit pada masing-masing kelompok kontrol dan kelompok perlakuan
fraksi klorofrom daun J. curcas setiap 1 jam selama 6 jam pengamatan

77

Lampiran VIII. Hasil analisis statistik pengamatan histopatologi jumlah neutrofil

per 100 sel leukosit pada kaki tikus Wistar yang di induksi
karagenan 1%

One-Sample Kolmogorov-Smirnov Test

neutrofil
N
Normal Parametersa,b

Most Extreme Differences

15
Mean

7,3333

Std. Deviation

4,18614

Absolute

,178

Positive

,178

Negative

-,143

Kolmogorov-Smirnov Z

,690

Asymp. Sig. (2-tailed)

,729
,661c

Sig.
Monte Carlo Sig. (2-tailed)

95% Confidence Interval

Lower Bound

,652

Upper Bound

,670

a. Test distribution is Normal.

b. Calculated from data.
c. Based on 10000 sampled tables with starting seed 2000000.

78

Oneway

Descriptives
neutrofil
N

Mean

Std.

Std.

95% Confidence Interval for

Deviation

Error

Mean

Minimum Maximum

Lower Bound Upper Bound

kontrol

12,3333

1,52753

,88192

8,5388

16,1279

11,00

14,00

kontrol positif

2,6667

2,30940

1,33333

-3,0702

8,4035

,00

4,00

FKDJP 150

5,0000

2,00000

1,15470

,0317

9,9683

3,00

7,00

FKDJP 300

10,6667

3,21455

1,85592

2,6813

18,6521

7,00

13,00

FKDJP 600

6,0000

1,73205

1,00000

1,6973

10,3027

5,00

8,00

15

7,3333

4,18614

1,08086

5,0151

9,6515

,00

14,00

negatif

Total

Test of Homogeneity of Variances

neutrofil
Levene Statistic
1,027

df1

df2
4

Sig.
10

,439

ANOVA
neutrofil
Sum of Squares
Between Groups
Within Groups
Total

df

Mean Square

195,333

48,833

50,000

10

5,000

245,333

14

F
9,767

Sig.
,002

79

Post Hoc Tests

Multiple Comparisons
Dependent Variable: neutrofil
LSD
(I) kelompok

(J) kelompok

Mean Difference

Std. Error

Sig.

(I-J)

kontrol negatif

kontrol positif

FKDJP 150

FKDJP 300

FKDJP 600

95% Confidence Interval

Lower Bound

Upper Bound

kontrol positif

9,66667*

1,82574

,000

5,5987

13,7347

FKDJP 150

7,33333*

1,82574

,002

3,2653

11,4013

FKDJP 300

1,66667

1,82574

,383

-2,4013

5,7347

FKDJP 600

6,33333*

1,82574

,006

2,2653

10,4013

kontrol negatif

-9,66667*

1,82574

,000

-13,7347

-5,5987

FKDJP 150

-2,33333

1,82574

,230

-6,4013

1,7347

FKDJP 300

-8,00000*

1,82574

,001

-12,0680

-3,9320

FKDJP 600

-3,33333

1,82574

,098

-7,4013

,7347

kontrol negatif

-7,33333*

1,82574

,002

-11,4013

-3,2653

kontrol positif

2,33333

1,82574

,230

-1,7347

6,4013

FKDJP 300

-5,66667*

1,82574

,011

-9,7347

-1,5987

FKDJP 600

-1,00000

1,82574

,596

-5,0680

3,0680

kontrol negatif

-1,66667

1,82574

,383

-5,7347

2,4013

kontrol positif

8,00000*

1,82574

,001

3,9320

12,0680

FKDJP 150

5,66667*

1,82574

,011

1,5987

9,7347

FKDJP 600

4,66667*

1,82574

,029

,5987

8,7347

kontrol negatif

-6,33333*

1,82574

,006

-10,4013

-2,2653

kontrol positif

3,33333

1,82574

,098

-,7347

7,4013

FKDJP 150

1,00000

1,82574

,596

-3,0680

5,0680

FKDJP 300

-4,66667*

1,82574

,029

-8,7347

-,5987

*. The mean difference is significant at the 0.05 level.

80

Lampiran IX. Dokumentasi Penelitian

Proses penguapan

Pemberian Karagenan

Penyaringan filtrat
pemberian sediaan uji
Ekstrak kental fraksi
kloroform
pengukuruan volume edema

Sediaan uji FKDJP berbagai dosis

Kaki tikus untuk preparat HE