Hunter Larson

IDENTIFICATION OF
UNKNOWN BACTERIA
BIO 150 LAB

Students Name: Hunter Larson

Unknown ID Number: 3
Genus & Species: Proteus vulgaris

Grade: _____

Hunter Larson
GUIDELINES
1.

All work will be done by individuals or teams. If working in teams: each

investigating student must turn in a report. You do not have to agree on
the tests interpretation or final result. You can ask each other or teams
for advice, but you will perform all your own work.

2.

All entries must be neat & legible.

3.

Perform only the experiments necessary to identify your unknown

bacterium; do not do confirmation tests without just cause.

4.

Media and test results may be listed by abbreviation.

5.

Dichotomous Key must include names of all bacteria being considered

until theyve been eliminated by appropriate testing; highlight (with
a highlighting marker) the test results which you obtained.

6.

If you did not encounter any difficulties, then you do not have to fill out
the last section of the report.

7.

Unknown report is due _________________ (beginning of Lab 13).

Hunter Larson

GRAM STAIN VERIFICATION

DATE
04/09/2015

COLOR

MORPHOLOGY

DESIGNATION

Pink

Gram Negative- Bacilli

Gram Negative

BIOCHEMICAL TESTS PERFORMED

DATE

TEST

MEDIA

INCUBATION
TEMPERATURE

INCUBATION
PERIOD

04/13/201
5

LF, GF, H2S Prod.

TSI

37oC

48 Hours

04/15/201
5

Production of Urease

Urea Agar

37oC

48 Hours

DATE

BIOCHEMICAL TEST RESULTS

MEDIA
DESCRIPTION OF RESULTS

REAGENTS
ADDED

RESULTS
SUMMARY

04.15.201
5

TSI

Red Slant, Yellow Butt with

Black Precipitate

N/A

LNF, GF+, G+,

H2S Prod.

04.17.201
5

Urea Agar

Media Turned Cerise Pink

N/A

Urease+

Hunter Larson

DICHOTOMOUS KEY
Gram(-) Enterics
Escherichia coli
Enterobacter cloacae
Klebsiella pneumoniae
Proteus vulgaris
Salmonella typhimurium
Shigella flexneri

Lactose Fermentation ( on TSI )

(+) LF

(-) LNF

Escherichia coli
Enterobacter cloacae
Klebsiella pneumonia

Proteus vulgaris
Salmonella typhimurium
Shigella flexneri
H2S Production ( on TSI )
(+)
Proteus vulgaris
Salmonella typhimurium

(-)
Shigella flexneri

Urea Agar

(+)
Proteus vulgaris

(-)
Salmonella typhimurium

Hunter Larson

DIFFICULTIES/PROBLEMS ENCOUNTERED
____N/A_________________________________________________________
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A grade category will first be assigned, based on the overall work
presented.
Outstanding Report:
A
Above Average Report: B
Average Report:
C
Below Average Report: D
Sub-standard Report:
F
Grading criteria used will include:

Neatness & Legibility

Appropriate Tests
Proper Interpretation
Following Instructions
Correct Identification

Hunter Larson
After that, a specific numeric grade will be assigned within the Letter
Grades 10 point range for your final grade for the Unknown Report.