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Transpiration Lab

Name: ______________________________________

Date: ___________

Objectives:

Tounderstandhowwatermovesfromrootstoleavesintermsofthe
physical/chemicalpropertiesofwaterandtheforcesprovidedbydifferencesinwater
potential.
Tounderstandtheroleoftranspirationinthetransportofwaterwithinaplant.
Tounderstandthestructuresusedbyplantstotransportwaterandregulatewater
movement.
Totesttheeffectsofenvironmentalvariablesonratesoftranspirationusinga
controlledexperiment.
Toidentifyxylemandphloemcells,andrelatethefunctionofthesevasculartissues
tothestructuresoftheircells.

Introduction:
SeeCollegeBoardLabManual

Procedure:
1. Wraptheentirerootballinaplasticbag,snugituptothestemwithstring,markwith
yourgroupnameornumber,andweighonMonday.Weigheachsuccessivedayforthe
entireweek.
2. Allgroupsputtheircontrolsinoneplace,thoseinfrontofthefantogether,thosein
brightlighttogether,andthelastaremistedandcoveredwithanotherplasticbagand
areplacedwiththecontrols.Darkareplacedinadrawerintheroom.
3. Ifyourplantblooms,pinchthebloomsoffandbesureanyleavesorbloomsthatfalloff
areputbackinthecenteroftheplanttobeweighedeachdaysoasnottorepresentwater
loss.
4. Writeahypothesisaboutwhatyouthinkwhatwillhappenbasedonyourknowledgeof
transpirationandplants.(1)
__________________________________________________________________________
__________________________________________________________________________

Results:
Fillinthedatatable.Besuretousethecorrectunitsformass.
DAY0

DAY1

DAY2

(5)

DAY3

DAY4

CONTROL
HUMIDITY
LIGHT
FAN
DARK

Determinethe%changeinmassovertheweekandgraph.Besureyourgraphhasallofthe
appropriatetitlesandunits. (8)
TITLE:________________________________________________________________

Analysis:(FromtheCollegeBoardLabManual)

(36pointstotal)

1. Forthisexperiment,whatwastheindependentvariable?Thedependentvariable?What
weretheconstants? (4)

2. Calculatetheaveragerateofwaterlossperminuteforeachofthetreatments.(4)
a. Humidity

b. Light

c. Fan

d. Dark

3. Explainwhyeachoftheconditionscausesanincreaseordecreaseintranspiration
comparedwiththecontrol. (4)
a. Humidity
b. Light
c. Fan
d. Dark
4. Howdideachconditionaffectthegradientofwaterpotentialfromthestemtoleafinthe
experimentalplant? (4)
a. Humidity
b. Light
c. Fan
d. Dark
5. Whatistheadvantagetoaplantofclosedstomatawhenwaterisinshortsupply?What
arethedisadvantages?
(3)

6. Describeseveraladaptationsthatenableplantstoreducewaterlossfromtheirleaves.
Includebothstructuralandphysiologicaladaptations.
(4)

7. Whydidyouneedtocalculatethe%waterlosseachdayinsteadofgraphingthetotal
amountofwaterlosteachday?
(1)

8. CalculatethetotalsurfaceareaofalltheleavesbyusingtheLeafMassMethoddescribed
intheCollegeBoardlab.
(1)

LeafSurfaceArea(m2)=__________________

9. Waterlosspersquaremeter:Tocalculatethewaterlosspersquaremeterofleafsurface,
dividethewaterloss(changeinmassingrams)ateachreadingbytheleafsurfaceyou
calculated.(Rememberthat1gram=1milliliter)
IndividualGroupWaterLossinmL/m2
DAY1

(5)
DAY2

DAY3

CONTROL
HUMIDITY
LIGHT
FAN
DARK

10. Recordtheaveragesoftheclassdataforeachtreatmentinthetablebelow.
ClassAverageCumulativeWaterLossinmL/m2(5)

DAY4

DAY1

DAY2

DAY3

CONTROL
HUMIDITY
LIGHT
FAN
DARK

11.Whydidyouneedtocalculateleafsurfaceareaintabulatingyourresults? (1)

DAY4

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